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Citations for New England Biolabs :
6801 - 6850 of 9358 citations for Rat Leptin RIA kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genomics 2022Quote: ... adapters and the Sau3AI overhang adapters (375 pM) in a 40 μL solution using 50 units of Quick Ligase (Quick Ligation Kit; NEB) that was 1X reaction buffer ...
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bioRxiv - Genomics 2022Quote: ... and 100 ng of each PCR product was used to generate the final libraries using the NEBNext Ultra II DNA Library Prep Kit for Illumina (New England Biolabs). The libraries were indexed for multiplexing using NEBNext multiplex oligos kit for Illumina (New England Biolabs) ...
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bioRxiv - Microbiology 2022Quote: Detection of viral genomes from heat-inactivated samples was performed by RT-qPCR using the Luna Universal One-Step RT-qPCR Kit (New England Biolabs) with SARS-CoV-2 specific primers targeting the N gene region (5′-TAATCAGACAAGGAACTGATTA-3′ and 5′-CGAAGGTGTGACTTCCATG-3′ ...
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bioRxiv - Microbiology 2022Quote: ... FastDigest restriction enzymes (Thermo-Fisher) were used for plasmid digestion and Gibson assembly was performed using the NEBuilder HiFi DNA assembly kit (NEB). All constructs were verified by DNA sequencing.
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bioRxiv - Neuroscience 2022Quote: ... 250 ng of the purified PCR product was processed with the NEBNext Ultra II FS DNA Library Prep Kit for Illumina (NEB) according to manufacturer protocols ...
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bioRxiv - Neuroscience 2022Quote: ... was amplified by PCR from the same vector and its DNA was extracted using the Monarch DNA Gel Extraction Kit (New England Biolabs). DNA segments for Fc regions ...
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bioRxiv - Neuroscience 2022Quote: ... Harvested RNA was prepared for sequencing with the NEBNext® Ultra™ II RNA Library Prep Kit for Illumina (NEB). Two biological replicates were performed for each condition ...
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bioRxiv - Neuroscience 2022Quote: ... Strand-specific RNA libraries were prepared using the NEBNext Ultra II Directional RNA Library Prep Kit for Illumina with NEBNEXT poly(A) mRNA Isolation Module (New England Biolabs) according to manufacturer recommendations ...
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bioRxiv - Neuroscience 2022Quote: ... Library preparation was performed using NEBNext® Ultra II Directional RNA Library Prep Kits for Illumina (New England Biolabs, USA), according to the manufacturer’s protocol with 8 PCR cycles ...
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bioRxiv - Molecular Biology 2022Quote: The DNase-treated RNA was used for cDNA synthesis utilizing the ProtoScript First Strand cDNA Synthesis Kit (NEB, Ipswich, MA) according to manufacturer’s protocols ...
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bioRxiv - Molecular Biology 2022Quote: In vitro transcription reactions were performed according to standard protocols provided with the high-yield in vitro transcription kits (E2040 and E2070, New England Biolabs), consisting of 40 mM rNTP (pH buffered with sodium phosphate ...
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bioRxiv - Molecular Biology 2022Quote: ... the corresponding promoter sequences were inserted in the DNA templates using Q5 Site-Directed Mutagenesis Kit (E0554, New England Biolabs). DNA templates encoding for functional mRNAs ...
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bioRxiv - Molecular Biology 2022Quote: ... Preparation of the sequencing libraries was performed using the NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (New England Biolabs). 150-bp paired-end sequencing was carried out using Illumina Novaseq 6000 ...
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bioRxiv - Molecular Biology 2022Quote: The cDNA synthesis was performed as described before with a modified cleanup step using Monarch PCR & DNA Cleanup Kit (T1030, New England Biolabs) [26] ...
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bioRxiv - Molecular Biology 2022Quote: ... The eluted DNA fragments were then amplified by PCR with Nextera compatible indexed sequencing i5 and i7 adapters using NEBNext 2x PCR Master Mix PCR kit (M0541, NEB). The amplified DNA library was fragment size selected from 200bp to 800bp using Ampure XP beads (A63880 ...
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bioRxiv - Neuroscience 2022Quote: ... The N533H and L534I mutations in the pEGFP-SFPQ and pGEX6P1-SFPQ-276–598 constructs were generated with the Q5 site-directed mutagenesis kit (New England Biolabs). All constructs were verified by DNA sequencing ...
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bioRxiv - Plant Biology 2022Quote: ... and 100 ng of bending site RNA samples from five-day-old Col-0 and myb93 plants using the NEBnext Ultra II RNA Library Prep kit (New England Biolabs) following manufacturer protocols ...
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Dual targeting factors are required for LXG toxin export by the bacterial type VIIb secretion systembioRxiv - Microbiology 2022Quote: ... Plasmids were then digested with BamHI and NotI and the deletion fragment was gel extracted (Monarch DNA gel extraction kit, NEB). 100ng of purified deletion fragment was then added to competent S ...
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bioRxiv - Microbiology 2022Quote: ... Libraries were prepared from sheared gDNA using the NEBNext Ultra II DNA Library Prep Kit for Illumina (New England Biolabs). Briefly ...
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bioRxiv - Microbiology 2022Quote: ... the concentration was measured by qPCR using a NEBNext Library Quant Kit for Illumina (New England BioLabs GmbH, Hessen, Germany), and the size was determined by Bioanalyzer 2100 using Agilent High Sensitivity DNA Kit (Agilent Technologies GmbH ...
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bioRxiv - Microbiology 2022Quote: ... human NINL isoform 2 (NCBI accession NM_001318226.2) and the NINL isoform 2 mutant (Q231R) were mutagenized using the Q5 Site-Directed Mutagenesis Kit (New England BioLabs). The plasmids encoding 3C proteases (coxsackievirus B3 (CVB3 ...
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bioRxiv - Microbiology 2022Quote: ... RNA was subjected to OneStep qRT-PCR analysis using the Luna Universal One-Step RT-qPCR Kit (New England Biolabs) or Luna Universal Probe One-Step RT-qPCR Kit (New England Biolabs ...
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bioRxiv - Molecular Biology 2022Quote: The library for RNA-seq was prepared with 200 ng RNA input and the NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (New England BioLabs (NEB), E7760L ...
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bioRxiv - Molecular Biology 2022Quote: ... or 0.1x TE and was used for library preparation using NEBNext Ultra II DNA library prep kit for Illumina (NEB, E7645) following manufacturer instructions ...
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bioRxiv - Molecular Biology 2022Quote: ... The RNA samples were directly proceeded for cDNA library preparation using NEBNext Ultra II directional RNA library kit (NEB, E7760) following the library preparation protocol ...
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bioRxiv - Molecular Biology 2022Quote: ... We added Illumina TruSeq adaptors using the NEBNext Ultra II DNA Library Prep Kit for Illumina (New England Biolabs: E7645) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2022Quote: ... were generated by deletion of the unwanted DNA from the parental plasmid using the Q5 Site-Directed Mutagenesis Kit (NEB BioLabs). The R763G point mutation was introduced into D1-A1 by PCR mutagenesis ...
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Characterization of novel Actinobacteriophage Giantsbane reveals unexpected cluster AU relationshipsbioRxiv - Genomics 2022Quote: ... Sequencing libraries were made using a NEBNext® Ultra™ II DNA Library Prep kit (New England Biolabs, MA, USA) and were sequenced using the Illumina MiSeq platform to 2197x coverage ...
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bioRxiv - Genomics 2022Quote: ... Ribosomal depleted RNA was used to make the RNAseq libraries using NEBNext Ultra Directional RNA Library Prep Kit for Illumina (NEB). All the libraries were checked through a Bio-analyzer for quality control purpose ...
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bioRxiv - Evolutionary Biology 2022Quote: ... A donor DNA containing two homology arms flanking the DsRed sequence for homology repair were cloned into the pHD-ScarlessDsRed plasmid (https://flycrispr.org/scarless-gene-editing/) using the Q5® High-Fidelity DNA Polymerase kit (New England Biolabs). Left homology arm contained the sequence in 2R:9870299-9871095 (FlyBase Release 6 ...
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bioRxiv - Immunology 2022Quote: ... and Illumina linker addition to B cell heavy chain transcripts were performed using the human NEBNext Immune Sequencing Kit (New England Biolabs) according to the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2022Quote: ... PolyA-selected libraries were made using NEBNext Poly(A) mRNA Magnetic Isolation Module and NEBNext Ultra Directional RNA Library Prep Kit (New England Biolabs) according to the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2022Quote: ... The ribosomal-depleted RNA was used to make the RNA-seq libraries using NEBNext Ultra Directional RNA Library Prep Kit for Illumina (E7760, NEB). These libraries were analysed through a Bioanalyzer for quality control ...
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bioRxiv - Synthetic Biology 2022Quote: ... NNN spacer to increase library diversity during sequencing (preadenylated oligos were prepared by 5’ DNA adenylation kit (E2610L) using thermostable 5’ App DNA/RNA ligase (M0319L, both from New England Biolabs) following the manufacturer’s protocol ...
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bioRxiv - Plant Biology 2022Quote: ... The resulting PCR product was cloned into pBUE411 vector at the PmeI cut site by Gibson Assembly using NEBuilder HiFi DNA Assembly Cloning Kit (New England BioLabs) and this produced the pBUE411_GRF-GIF construct (Figure 1a).
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bioRxiv - Plant Biology 2022Quote: ... was commercially synthesized (Tsingke) and ligated into the pCA2300-LbCpf1 vector through HindIII site using the T4 DNA ligase kit (NEB).
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bioRxiv - Zoology 2022Quote: ... Stranded cDNA libraries were created with the NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (New England Biolabs). Fish were sequenced for 100 base pair reads on one lane of a NovaSeq 6000 (Illumina) ...
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bioRxiv - Zoology 2022Quote: ... The T7 reverse-complement sequence was annealed to the probe sets to allow transcription into RNA and biotinylation in a single reaction using HiScribe T7 High Yield RNA Synthesis Kit (New England Biolabs) followed by a Dnase treatment to avoid sample contamination by probe DNA during the capture ...
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bioRxiv - Zoology 2022Quote: ... DNA libraries were prepared using a NEBNext Ultra II DNA Library Prep Kit and NEBNext multiplex Oligos for Illumina (New England Biolabs). BLV-specific probes were used for the enrichment of DNA libraries with BLV sequence and the enriched libraries were PCR amplified using the primer set P5-P7 ...
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bioRxiv - Physiology 2022Quote: cDNA synthesis was completed using a NEB First Strand cDNA synthesis kit (M0368; New England Biolabs Canada, Whitby, ON, Canada). Briefly ...
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bioRxiv - Physiology 2022Quote: RNA-sequencing libraries were prepared by depleting eukaryotic ribosomal RNA with the NEBNext rRNA Depletion Kit prior to library synthesis with the NEBNext Ultra II Directional RNA Library Prep Kit for Illumina and addition of multiplex oligos using the Unique Dual Index Primer Pairs Set 3 (NEB). Library quality control was performed using Qubit and Bioanalyzer 2100 ...
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bioRxiv - Physiology 2022Quote: ... RNA-seq libraries were generated with the NEBNext Ultra II Directional RNA Library Prep Kit coupled with Poly(A) mRNA Magnetic Isolation Module and NEBNext multiplex oligos for Illumina (New England Biolabs). Deep sequencing was performed on the NextSeq 500 sequencing system (Illumina ...
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bioRxiv - Microbiology 2022Quote: ... The samples were sheared to around 450-bp fragments and the library constructed using the NEBNext UltraII DNA library kit (NEB), followed by qPCR for sample pooling and normalisation for the Illumina sequencing platform ...
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bioRxiv - Microbiology 2022Quote: ... Site-directed mutagenesis was used to introduce deletions and substitutions into expression vectors using the Q5 site directed mutagenesis kit (New England Biolabs) and according to manufacturer’s instructions ...
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bioRxiv - Bioengineering 2022Quote: ... 500 ng of total RNA was used to synthesize cDNA using the LunaScript RT SuperMix Kit (New England Biolabs, E3010L). The Luna Universal qPCR Master Mix (New England Biolabs ...
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bioRxiv - Cell Biology 2022Quote: ... RNA was converted to double stranded cDNA using random hexamer with the NEBNext Ultra RNA First Strand and NEBNext Ultra RNA 2nd Strand Synthesis Kits (NEB). 1D library was prepared with the SQK-LSK108 kit (Oxford Nanopore ...
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bioRxiv - Microbiology 2022Quote: ... 12 µl template DNA was supplemented with the required reagents from the NEBNext Ultra II End Repair/dA Tailing kit (E7546S, NEB) and was first incubated at 20°C for 5 minutes and then at 65°C for 5 minutes ...
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bioRxiv - Molecular Biology 2022Quote: ... Libraries were prepared and sequenced by the Edinburgh Clinical Research Facility using NEBNEXT Ultra II Directional RNA Library Prep kit (NEB) and Poly-A mRNA magnetic isolation module (NEB) ...
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bioRxiv - Cancer Biology 2022Quote: ... and the eluates were treated with proteinase K for 16 h at 65°C before library synthesis using NEBNext Ultra II DNA library preparation kits (New England Biolabs). The samples were sequenced on HiSeq 2000 (Illumina) ...
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bioRxiv - Cell Biology 2022Quote: ... A biotin pull down was then performed on the size-selected fragments using streptavidin-coated beads before library preparation on the beads using the standard NEBNext Ultra II DNA library prep kit for Illumina (NEB) protocol ...