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Citations for New England Biolabs :
6801 - 6850 of 9501 citations for FabFc ZAP rat Antibody Internalization Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2021Quote: ... A plasmid encoding an eGFP-containing EBOV antigenome was generated through assembly of fragments using the NEBuilder HiFi DNA assembly cloning kit (New England Biolabs (NEB)) ...
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bioRxiv - Molecular Biology 2021Quote: ... tropicalis pseudouridine synthase Pus7 was amplified from total RNA using the OneTaq One-step RT-PCR kit (New England Biolabs). XmaI and XhoI restriction sites were added with oligonucleotides and the resulting fragment was cloned into the p415Gal1 vector.
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bioRxiv - Immunology 2021Quote: ... and the RNA-seq libraries were generated with NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (NEB, E7760) according to manufacturer’s instructions.
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bioRxiv - Microbiology 2021Quote: ... Point mutations were introduced in the P and N sequences by site-directed mutagenesis using the Q5 site-directed mutagenesis Kit (New England Biolabs). Sequence analysis was carried out to check the integrity of all constructs ...
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bioRxiv - Microbiology 2020Quote: ... TEV was removed from the original plasmid and mEGFP was inserted in its place using a Gibson reaction via the HiFi DNA Assembly cloning kit (New England Biolabs).
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bioRxiv - Developmental Biology 2021Quote: ... each CBE4max-SpG and CBE4max-SpRY sequence has been inserted into pCS2+ plasmid linearized with EcoRI using the Gibson Assembly Cloning Kit (New England Biolabs). The fragment has been amplified using the primers F-5’-TGCAGGATCCCATCGATTCGGCCACCATGAAACGGACAG -3’ and R-5’-TAGAGGCTCGAGAGGCCTTGTCAGACTTTCCTCTTCTTCTTGG -3’ ...
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bioRxiv - Immunology 2021Quote: ... ii) fragmentation and adapter ligation (NEBNext® UltraTM II FS DNA Library Prep Kit for Illumina, NEB, cat. no. E7805S), and iii ...
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bioRxiv - Cell Biology 2021Quote: ... Stranded mRNA-Seq libraries were prepared from 250 ng of total RNA using the NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (New England Biolabs) implemented on the liquid handling robot Beckman i7 ...
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bioRxiv - Cell Biology 2021Quote: ... 100 ng of total RNA was used for library synthesis with the NEBNext Ultra II Directional RNA Library Preparation kit (New England Biolabs) according to the protocol of the manufacturer ...
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bioRxiv - Genomics 2021Quote: ... Libraries were prepared with the NEB Next® Ultra™ DNA Library Prep Kit (New England Biolabs, Ipswich MA, USA) and sequenced on an Illumina NovoSeq 6000 PE150 platform at Novogene (Hong Kong ...
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bioRxiv - Genomics 2021Quote: ... 25ng of DNA was used to prepare sequencing libraries using NEBNext® Ultra™ DNA Library Prep Kit (NEB, E7370L) according to manufactures manual ...
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bioRxiv - Genomics 2021Quote: ... 1 µg total RNA was subjected to cDNA synthesis applying the ProtoScript® First Strand cDNA Synthesis Kit (NEB, E6300S) with the included Oligo d(T)23 VN primer according to the manufacturer’s instructions ...
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bioRxiv - Genomics 2021Quote: All samples that met the previous criteria were submitted to cDNA synthesis protocol using LunaScript™ RT SuperMix Kit (NEB) following the manufacturer’s instructions ...
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bioRxiv - Genomics 2021Quote: The poly(A) RNA library was obtained using the NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (NEB) including the NEBNext Poly(A ...
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bioRxiv - Genomics 2021Quote: The pCeTyX Ty1hygro-AI carrying plasmid was constructed by assembling four overlapping DNA fragments thanks to the Gibson Assembly Cloning Kit (NEB). The 5,472 bp plasmid backbone was obtained after restriction of p41Neo 1-F GW (https://www.addgene.org/58545/ ...
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bioRxiv - Genomics 2021Quote: ... The isolated mRNA was then used to prepare sequencing libraries using NEBNext Ultra II Non-directional RNA Library Prep Kit for Illumina and NEBNext Multiplex Oligos for Illumina (Index sets 1 and 2) (NEB). Next ...
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bioRxiv - Genomics 2020Quote: ... and 150ng of each sample were fed to library preparation with NEBNext Ultra II DNA Library Preparation kit for Illumina (New England BioLabs) according to manufacturer’s instruction ...
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bioRxiv - Genomics 2021Quote: ... 30 seconds at 65°C and 30 seconds at 72°C using the NEBNext® Multiplex Oligos kit (NEB, UK). The expected library size was ∼200-250bp ...
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bioRxiv - Genomics 2021Quote: ... Sequencing libraries were generated using the NEBNext® UltraTM RNA Library Prep Kit for Illumina® (NEB, Ipswich, MA, USA), following the manufacturer’s recommendations ...
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bioRxiv - Genetics 2020Quote: ... Paired-end libraries were prepared by the NEBNext® Ultra™ DNA Library Prep Kit for Illumina® (NEB, USA) and then sequenced on the Illumina HiSeq2500 platform after quantification ...
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bioRxiv - Genetics 2020Quote: ... Paired-end libraries were prepared by the NEBNext® Ultra™ RNA Library Prep Kit for Illumina® (NEB, USA) and sequenced on the Illumina HiSeq2500 platform after quantification ...
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bioRxiv - Genetics 2020Quote: ... RNA sequencing libraries were prepared using the NEB Next Ultra RNA Library Prep Kit for Illumina using manufacturer’s instructions (NEB, USA). Briefly ...
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bioRxiv - Microbiology 2021Quote: ... 1 kb of upstream and downstream DNA were cloned into the suicide vector pK18mobsacB by Gibson assembly45 using the NEBuilder HiFi DNA Assembly Cloning Kit (New England Biolabs). The vector was introduced into B ...
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bioRxiv - Cell Biology 2021Quote: ... using 35 cycles with an annealing temperature of 60 °C (see table 2 for list of primers) and cloned by Gibson Assembly Cloning Kit (EE5510S, NEB). All primers were designed using SnapGene (GSL Biotech LLC ...
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bioRxiv - Biochemistry 2020Quote: ... Mature tRNA sequences were obtained by deleting the intron sequence using the Q5 Site-Directed Mutagenesis kit (New England Biolabs). All constructs in this study were verified by Sanger sequencing.
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bioRxiv - Genomics 2020Quote: ... we prepared libraries of circle-enriched and whole genomic control samples using NEBNext FS DNA Ultra II Library Prep Kit (New England Biolabs) using protocol modifications outlined in (Sproul and Maddison 2017) ...
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bioRxiv - Molecular Biology 2021Quote: ... The corresponding non-binding mutant YESubB (S31A; Y100F) was engineered from the wt template above using the Q5 Site-Directed Mutagenesis Kit (New England BioLabs) and sequence verified.
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bioRxiv - Molecular Biology 2019Quote: ... This was assembled with PfoI-linearized pRS426 using the NEBuilder® HiFi DNA Assembly Cloning Kit (New England Biolabs, E5520S).
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bioRxiv - Molecular Biology 2022Quote: The pre-crRNA was transcribed in vitro using HiScribe™ T7 Quick High Yield RNA Synthesis Kit (NEW ENGLAND BioLabs). Transcription template (dsDNA ...
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bioRxiv - Microbiology 2022Quote: ... PCRs purified using the Nucleospin gel and PCR clean-up kit were cloned into plasmid backbones digested with restriction enzymes (NEB) using the In-Fusion HD cloning system (Takara) ...
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bioRxiv - Developmental Biology 2022Quote: Libraries were generated using the NEBNext® Ultra™ II Directional RNA Library Prep kit for Illumina® (NEB #7760L) with 2.5 ng RNA input (1/300 adaptor dilution ...
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bioRxiv - Developmental Biology 2022Quote: ... HDR plasmid with mutant me31B genes was generated by mutating the me31B wild type gene in the wildtype HDR donor plasmid by using the Site-Directed Mutagenesis kit (New England Biolabs) according to the manufacturer’s recommended protocols ...
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bioRxiv - Cancer Biology 2022Quote: ... 5ng of DNA was used for library preparation using NEBNext Ultra II DNA Library Prep Kit for Illumina (NEB, E7645S), SPRIselect beads (Beckman Coulter ...
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bioRxiv - Cell Biology 2022Quote: MKLP1(1-711)-3xFLAG-Avi was cloned by stitching four oligonucleotide primer sequences together into a digested MKLP1(1-711)-Avitag plasmid using a HiFi DNA assembly reaction kit (M5520A, New England Biolabs). Ten 10cm plates were seeded with COS-7 cells and each plate was co-transfected 24 hours later with 4.08 μg MKLP1(1-711)-3xFLAG-Avi and 4.08 μg HA-BirA plasmids using TransIT-LT1 transfection reagent (Mirus) ...
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bioRxiv - Cell Biology 2022Quote: ... ribosomal RNA was depleted from 300 ng of the total RNA samples using the NEBNext rRNA Depletion Kit (NEB, E6310L), then processed for RNA-seq library prep with the NEBNext Ultra II Directional RNA Library Prep Kit (NEB ...
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bioRxiv - Microbiology 2022Quote: ... enzymatic shearing was performed on 500ng of genomic bacterial DNA using the NEBNext Fast DNA Library Prep kit (New Englands Biolabs) and following the manufacturer’s recommendations ...
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bioRxiv - Cancer Biology 2022Quote: ... RNAseq libraries were created from 250ng of total RNA using the NEBNext UltraII Directional RNA Library Prep kit (New England Biolabs) using half volume reagents and 14 cycles of PCR ...
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bioRxiv - Molecular Biology 2022Quote: RNA-seq libraries were prepared using the NEBNext Poly(A) mRNA Magnetic Isolation Module and NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (New England Biolabs). Samples were multiplexed using the NEBNext Multiplex Oligos for Illumina (NEB) ...
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bioRxiv - Cell Biology 2022Quote: ... DNA was purified using standard Phenol:chloroform:isoamyl alcohol extraction and ethanol precipitation and used for library preparation with the NEBNext Ultra II DNA Library Prep Kit for Illumina (NEB) with 9 (H3K4me1 ...
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bioRxiv - Microbiology 2022Quote: ... Strand-specific CHIKV RNA primers were chosen from previous work [54] to amplify nucleotides 1-1560 of the CHIKV genome and used in reverse transcription reactions with the LunaScript8482 RT SuperMix Kit (NEB), followed by qPCR using the 2x qPCRBIO SyGreen Blue Mix (PCRBIOSYSTEMS) ...
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bioRxiv - Molecular Biology 2022Quote: ... PolyA + fraction was isolated from 4.5 μg of DNAse-treated total RNA using NEBNext Oligo d(T)25 Magnetic beads kit (NEB, USA), according to manufacturer’s recommendations ...
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bioRxiv - Molecular Biology 2022Quote: ... The Hi-C library for Illumina sequencing was prepared with the NEBNext Ultra II DNA Library Prep Kit for Illumina (NEB) according to the manufacturer’s instructions ...
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Co-stimulatory molecules decide T cell fate through regulations of their invigoration and impairmentbioRxiv - Molecular Biology 2022Quote: ... mRNA sequencing libraries were prepared with 500 ng of total RNA of each sample using the NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (NEB) with NEBNext Poly(A ...
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bioRxiv - Molecular Biology 2022Quote: ... PCR amplicons were verified by agarose gel electrophoresis and purified using the Monarch PCR and DNA Cleanup Kit (New England Biolabs). In vitro transcription was performed using the T7 RiboMAX Express Large Scale RNA Production System (Promega) ...
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bioRxiv - Molecular Biology 2022Quote: ... and BRD4L-MU34 PAS reporters were generated by site-directed mutagenesis of TRIM9S-WT or BRD4L-WT PAS reporters using Q5 Site-Directed Mutagenesis Kit (New England Biolabs).
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bioRxiv - Bioengineering 2022Quote: ... HiScribe T7 RNA polymerase kits and Q5 2x HiFidelity PCR mastermixes were purchased from New England Biolabs (NEB, Ipswitch, MA). Sodium cacodylate solution ...
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bioRxiv - Cancer Biology 2022Quote: ... Total RNA was used to generate barcoded RNA-seq libraries using the NEBNext Ultra II Directional RNA Library preparation kit (New England Biolabs) according to manufacturer’s instructions ...
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bioRxiv - Genomics 2022Quote: ... After 25 cycles of amplification the product was purified with the Monarch PCR&DNA Cleanup kit (New England BioLabs T1030L) and eluted with 20 μl of ddH2O ...
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bioRxiv - Biochemistry 2022Quote: MBOAT7 mutants were generated by site-directed mutagenesis on the pFBM construct expressing MBOAT7 with a C-terminal GFP and strep tag using the Q5 Mutagenesis Kit (New England Biolabs), according to the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2022Quote: ... Total RNA was used to generate barcoded RNA-seq libraries using the NEBNext Ultra RNA Library preparation kit (New England Biolabs). First ...