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Citations for New England Biolabs :
6601 - 6650 of 9501 citations for FabFc ZAP rat Antibody Internalization Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genetics 2019Quote: ... RNA-seq libraries were then prepared from 200 ng of RNA using the NEBNext Ultra II Directional RNA-seq kit (NEB). To quantitate the consistency of spike-in cell mixing for each individual sample ...
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bioRxiv - Genomics 2019Quote: ... extracted RNA without rRNA depletion was directly subjected to library preparation with the NEBNext Small RNA Library Prep Set kit (New England Biolabs) and sequenced on a HiSeq2000 (Illumina ...
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bioRxiv - Synthetic Biology 2020Quote: ... and used as input for library preparation using NEBNext Ultra II DNA Library Prep Kit (New England Biolabs, cat. E7645S), according to manufacturer instructions.
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bioRxiv - Cancer Biology 2020Quote: ... DNA fragments were purified from the reaction using a Zymo DNA Clean & Concentrator-5 kit (in-house) and amplified with NEBNext High Fidelity PCR Mix (NEB). Library quality was assessed using a Fragment Analyzer system (Agilent) ...
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bioRxiv - Molecular Biology 2020Quote: ... prior to preparing cnRNA-seq libraries using the NEBNext Ultra (for Bap1fl/fl and PRC1CPM ESCs) or Ultra II (for PRC1CPM;Bap1fl/fl ESCs) Directional RNA Library Prep kit (NEB). To quantitate the consistency of spike-in cell mixing for each individual sample ...
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Combinations of maternal-specific repressive epigenetic marks in the endosperm control seed dormancybioRxiv - Molecular Biology 2020Quote: ... and cDNA libraries were constructed using the NEBNext® Ultra™ II RNA Library Prep Kit for Illumina® (NEB). Libraries were sequenced on Illumina NovaSeq platforms as paired-end through Novogene mRNA sequencing services ...
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bioRxiv - Molecular Biology 2020Quote: ... The sequence libraries were generated using the NEBNext Ultra II DNA Library Prep Kit for Illumina (E7645, New England Biolabs) following the manufacturer’s instructions ...
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bioRxiv - Synthetic Biology 2020Quote: ... Colony PCR was used to screen surviving transformants for the presence of the 520-522 insert using primers spanning the insert junctions and amplification with Taq 2X Master Mix PCR Kit (New England Biolabs), as described by the supplier ...
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bioRxiv - Genomics 2020Quote: ... following the manufacturer’s instructions for the kit NEBNext® Ultra™ II DNA (New England Biolabs Inc. Ipswich, MA, USA). The libraries were sequenced using an Illumina NextSeq500 system (s/n NB501764).
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bioRxiv - Developmental Biology 2020Quote: ... Sequencing libraries were made with the fragmented DNA using a NEBNext Ultra DNA Library Prep Kit for Illumina according to the manufacturer’s instructions (cat. #E7370, New England Biolabs, USA). Library quality inspection was established via a LabChip GX Touch (PerkinElmer ...
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bioRxiv - Biochemistry 2020Quote: ... lpg1621 and lpg1949 were amplified by PCR from genomic DNA (kind gift from A. Hamprecht, University Hospital of Cologne) using the Phusion High Fidelity Kit (New England Biolabs). JOSD2 and UCHL3 were amplified from HEK293-derived cDNA accordingly ...
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bioRxiv - Systems Biology 2019Quote: ... followed by library preparation using the NEBNext Poly(A) Magnetic Isolation Module and NEBNext Ultra RNA sequencing library prep kit for Illumina (NEB). At the time of RNA isolation for the clones ...
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bioRxiv - Developmental Biology 2020Quote: ... We previously generated a human LRP5-V5 plasmid [32] and we used the Q5 site-directed mutagenesis kit (New England Biolabs) with forward primer (CTCAATGGCACATCCCGG ...
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bioRxiv - Molecular Biology 2019Quote: ... DNase-treated samples were dissolved in 10 μl water and used for sequencing library preparation using the NEBNext Directional Ultra RNA kit and Indexing primers (E7530, and E7335, respectively, both NEB) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2019Quote: ... DNase-treated samples were dissolved in 10 μl water and used for library preparation using the NEBNext Ultra II Directional RNA Library Prep Kit and Indexing primers (E7760, and E7335, respectively, both NEB) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2019Quote: ... Purified ChIP DNA was used for library generation using the NEBNext Ultra II DNA Library Prep Kit for Illumina (NEB) following manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2020Quote: ... The sgRNA was constructed by annealing sense and anti-sense single stranded oligonucleotides containing 5’ Bsa1 restriction overhangs and was inserted into Bsa1 linearized pDR274 with the Quick Ligase Kit (NEB) (Table 1) ...
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bioRxiv - Cell Biology 2020Quote: ... The kinase-inactive mutant was generated by a two-step PCR method using the Phusion High-fidelity kit (New England Biolabs). In the first step ...
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bioRxiv - Cell Biology 2020Quote: ... The AP-3 sorting mutant was regenerated in MigR1-PI4KIIα-GFP using the Q5 site-directed mutagenesis kit (New England Biolabs) following manufacturer’s instructions ...
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bioRxiv - Physiology 2020Quote: ... RNA with RIN>8 was used to prepare transcriptomic libraries using the NEBNext Ultra RNA library prep kit (New England Biolabs). High throughput RNA-Sequencing was performed at the NIDDK Genomic Core Facility (NIH ...
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bioRxiv - Synthetic Biology 2019Quote: ... Mutagenesis was performed using the partial overlapping primer design method or using a Q5 Site-Directed Mutagenesis Kit (New England Biolabs).
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bioRxiv - Genomics 2019Quote: ... We prepared D3839 libraries using the NEBNext Ultra II DNA Library Prep Kit for Illumina (New England Biolabs: E7645S/L) according to the manufacturer’s protocol ...
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bioRxiv - Genomics 2020Quote: ... ethanol purified used to produce barcoded RNA sequencing libraries using the NEBNext Ultra II Directional RNA Library Prep kit (New England Biolabs). Libraries were sequenced on Illumina NextSeq 500.
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bioRxiv - Genomics 2020Quote: ... and used to produce barcoded RNA sequencing libraries using the NEBNext Ultra II Directional RNA Library Prep kit (New England Biolabs). Libraries were sequenced on Illumina NextSeq 500.
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bioRxiv - Molecular Biology 2019Quote: ... and library preparation was completed using NEBNext® Ultra Directional RNA Library Prep Kit for Illumina® (New England Biolabs), both following manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2020Quote: ... ChIP sequencing libraries were prepared using the NEBNext Ultra II DNA Library Prep Kit for Illumina (New England Biolabs E7645S) with dual indexing (New England Biolabs E7600S ...
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bioRxiv - Cancer Biology 2020Quote: ... eluted in 8 μl and in vitro transcribed (with the beads) at 37 °C overnight for linear amplification using the T7 High Yield RNA polymerase IVT kit (NEB). Following IVT ...
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bioRxiv - Developmental Biology 2020Quote: ... Libraries were prepared using the NEBNext® Ultra II DNA Library Prep Kit for Illumina® (New England BioLabs, E7645) with starting input of 500ng of total RNA per sample ...
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bioRxiv - Genetics 2020Quote: ... Isolated mRNA was fragmented and thereafter reverse transcribed to cDNA using NEBNext ultra RNA library preparation kit (New England Biolabs). The cDNA products were purified using AmpureXP beads (Beckman Coulter ...
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bioRxiv - Genetics 2021Quote: ... ChIP-seq DNA libraries were prepared from the purified ChIP DNA and input DNA using the NEBNext ChIP-seq Library Prep Kit (E6200, NEB). Libraries were prepared from 8-15 ng of fragmented ChIP or input DNA ...
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bioRxiv - Plant Biology 2020Quote: ... The RNA-seq libraries were generated using NEBNext® Ultra™ RNA Library Prep Kit for Illumina® (NEB, USA) according to the manufacturer’s instructions and the high-throughput sequencing was performed with the Illumina NovaSeq6000 platform ...
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bioRxiv - Synthetic Biology 2019Quote: ... The synthesized RBS-Bdh-FLAG insert was resuspended in water to a final concentration of 10 ng/µL and was assembled with linearized pBBR1-MCS2 plasmid using NEBuilder High Fidelity DNA assembly kit (New England Biolabs) using 50 ng of pBBR1-MCS2 and 100 ng of RBS-Bdh-FLAG insert ...
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bioRxiv - Biochemistry 2019Quote: ... expression constructs containing WT or mutant full-length human KAP1 preceded by a triple FLAG tag and linker sequence MDYKDHDGDYKDHDIDYKDDDDKGSGG were assembled in pLEXm (35) with the NEBuilder HiFi DNA Assembly Cloning Kit (New England BioLabs). The following plasmids were used ...
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bioRxiv - Plant Biology 2021Quote: ... All other mutations of the constructs used for in vitro and in vivo assays were introduced using a Q5 site-directed mutagenesis kit (NEB). All constructs were verified by DNA sequencing.
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bioRxiv - Molecular Biology 2020Quote: ... PCR mutagenesis to create site-directed mutants of malM 3’-UTR and cspE 3’-UTR was conducted with the Q5 site-directed mutagenesis kit (New England Biolabs) using end-to-end primers designed with NEBaseChanger ...
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bioRxiv - Molecular Biology 2020Quote: ... 20 μl cDNA reaction mix was amplified by adding 25 μl of Long Amp Taq 2x Master Mix (NEB-kit), 1.25 μl SR primer for Illumina (NEB-kit) ...
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bioRxiv - Neuroscience 2021Quote: ... Sequencing adaptors were then added on using NEBNext Ultra II DNA Library Prep Kit for Illumina (New England Biolabs #E7645S) following the manufacturer’s instructions for a 200 bp insert size distribution and using AMpure XP beads (Beckman Coulter ...
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bioRxiv - Molecular Biology 2021Quote: ... Small RNA libraries were generated using the NEBnext small RNA library preparation kit according to instructions (E7580S, New England BioLabs).
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bioRxiv - Microbiology 2020Quote: ... All pcDNA3.1 P deletion mutants in the full-length construct were generated by using the Q5 site-directed mutagenesis kit (New England BioLabs), following the manufacturer recommendations ...
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bioRxiv - Genetics 2020Quote: ... The RNA was then diluted 1:50 and 2 mL were used to perform a one-step qPCR protocol using Luna Universal One-step qPCR kit (NEB). Two primer sets were used ...
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Increased ACTL6A Occupancy Within mSWI/SNF Chromatin Remodelers Drives Human Squamous Cell CarcinomabioRxiv - Molecular Biology 2021Quote: The libraries were generated by using the NEBNext ultraII DNA library prep kit for Illumina coupled with NEBNext multiplex oligos for Illumina (New England Biolabs) with few modifications optimized for small fragments (detailed in dx.doi.org/10.17504/protocols.io.wvgfe3w. ...
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Increased ACTL6A Occupancy Within mSWI/SNF Chromatin Remodelers Drives Human Squamous Cell CarcinomabioRxiv - Molecular Biology 2021Quote: RNA-seq libraries were generated by using NEBNext ultra II directional RNA library prep kit coupled with NEBNext multiplex oligos for Illumina (New England Biolabs) and following the manufacturer’s directions ...
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bioRxiv - Plant Biology 2021Quote: ... or the 2 kb region upstream of the translational start site of CaPR4 were inserted in front of the GUS reporter gene in vector pRB-bar-GUS using the NEBuilder recombinational cloning kit (NEB).
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bioRxiv - Synthetic Biology 2021Quote: ... an equal volume of RNA lysis buffer was added and RNA isolated using Monarch Total RNA Miniprep Kit (New England Biolabs). Each sample was treated by DNase I twice ...
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bioRxiv - Synthetic Biology 2021Quote: ... We ligated the vector with the inserts through Gibson assembly (Gibson et al., 2009) using NEBuilder HiFi DNA assembly kit (New England Biolabs). All primers and oligos used including sequencing primers (Integrated DNA Technologies ...
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bioRxiv - Synthetic Biology 2021Quote: ... These four oligos were then assembled with PCR amplified pUA66 vector using NEBuilder HiFi DNA assembly kit (New England Biolabs). The resulting pMV001 plasmid assembly mix was then used to electroporate Top10 E ...
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bioRxiv - Synthetic Biology 2021Quote: ... cell pellets were washed 1× with PBS and collected in 1X DNA/RNA protection reagent (Monarch Total RNA Miniprep Kit, NEB). Cells were lysed and total RNA was isolated following the mammalian cell protocol inlcuding on-column DNase I treatment ...
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bioRxiv - Physiology 2021Quote: ... The in vitro transcription of ayRhp1 capped mRNA (cRNA) was performed with HiScribe T7 ARCA mRNA kit (New England Biolabs) on SphI linearized pGEM-HE-ayRhp1 vector followed by column purification (RNeasy MiniElute Cleanup Kit ...
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bioRxiv - Evolutionary Biology 2021Quote: Library preparation was done with the NEBNext Ultra II DNA Library Prep Kit for Illumina (New England Biolabs, MA, USA) following the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2021Quote: ... chromatin from both IP and ‘Input’ samples were eluted and de-crosslinked at 65°C for 16 hours followed by DNA isolation by PCR and DNA cleanup kit (NEB). Enrichment of AR and MED1 at specified enhancers were quantified by qPCR using specific primers ...