Labshake search
Citations for New England Biolabs :
6551 - 6600 of 9358 citations for Rat Leptin RIA kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Genetics 2021Quote: Plasmid pNR127.9 was constructed by changing the first rfk-1 stop codon in pNR9.1 from TAG to TGG with primer set 1727/1728 and the Q5 Site-Directed Mutagenesis Kit (New England Biolabs). Similarly ...
-
bioRxiv - Genetics 2020Quote: ... The 5’ and 3’ sequences were amplified from WT adult gDNA extracted using the NucleoSpin Tissue Kit (Machery-Nagel) by PCR using Phusion High Fidelity PCR Master Mix (NEB) and primers 5’-ttttgcggccgcTCTGTTTGAATATGTTTCCGAGAA −3’ and 5’-ttttctcgagTTTCCGCGACAAAAACACAGA-3’ which add restriction enzyme recognition sites NotI and XhoI ...
-
bioRxiv - Evolutionary Biology 2021Quote: Libraries were prepared using the NEBNext Ultra II DNA Library Prep Kit (New England Biolabs, Ipswich, MA, USA; Fig. 2). Size selection was not employed for samples with highly degraded DNA ...
-
bioRxiv - Cell Biology 2021Quote: ... and 1 μg of DNase treated RNA was then taken for cDNA synthesis using the Protoscript I first strand cDNA synthesis kit (New England Biolabs). Selected genes were amplified by quantitative real time PCR (RT-qPCR ...
-
bioRxiv - Cell Biology 2021Quote: Plate-based scRNA-seq was performed with the NEBNext Single Cell/Low Input RNA Library Prep Kit for Illumina (catalog no. E6420L; New England Biolabs). Total cell fractions from dissociated gut sections of donors BRC2033-2034 ...
-
bioRxiv - Cell Biology 2021Quote: RNA used for polyA selected library generation for mRNA sequencing was isolated with the Monarch Total RNA isolation miniprep kit (New England Biolabs). Libraries were constructed using the Nugen Universal Plus mRNA-SEQ library construction kit (Nugen 0508 ...
-
bioRxiv - Genomics 2021Quote: ... and the PCR products were inserted into the SmaI site of the pPK6 vector (78) using a Gibson assembly cloning kit (New England Biolabs). This allows the expression of cloned genes under the control of the GAL1 promoter ...
-
bioRxiv - Genomics 2021Quote: ... The PCR products were inserted into the XbaI site of the pBP7 vector (78) using a Gibson assembly cloning kit (New England Biolabs). The cloned genes are placed downstream of the GAL1 promoter in the resulting plasmid constructs ...
-
bioRxiv - Plant Biology 2021Quote: ... and paired-end sequencing libraries were constructed by the NEBNext Ultra DNA Library Prep Kit II for Illumina (E7370S, NEB). DNA libraries were validated again on the Agilent TapeStation 4150 ...
-
bioRxiv - Immunology 2021Quote: For RNA-seq library preparation 2ug of total RNA was used to isolate mRNA using magnetic beads with mRNA isolation kit (PolyA mRNA isolation module, NEB). Later mRNA library preparation kit ...
-
bioRxiv - Physiology 2021Quote: ... RNA (0.5-1 μg) was reverse-trancribed to cDNA using LunaScript RT SuperMix Kit (New England Biolabs, Ipswich, MA, USA). TaqMan gene expression assays with FAM-labeled probes were purchased from ThermoFisher Scientific (Carlsbad ...
-
bioRxiv - Biochemistry 2021Quote: ... Input and RPF RNA samples were used to prepare libraries for sequencing using the NEBNext Small RNA Library Prep Kit (NEB) per manufacturer’s instructions ...
-
bioRxiv - Evolutionary Biology 2021Quote: ... RAD fragments were enriched with a streptavidin bead pull-down and prepared as a sequencing library using a NEBNext Ultra DNA Library Prep Kit (New England Biolabs). Final libraries were sequenced using paired-end 150 bp sequencing on an Illumina HiSeq 4000 at Duke University's Center for Genomic sand Computational Biology sequencing facility.
-
bioRxiv - Cell Biology 2021Quote: Genotyping PCR was performed on genomic DNA (isolated using a PureLink™ Genomic DNA Mini Kit according to manufacturer’s protocol) with Phusion (NEB) or taq (Invitrogen ...
-
bioRxiv - Molecular Biology 2020Quote: ... DNA libraries were generated by the Skoltech Genomics Core Facility using NEBNext Ultra II DNA Library Prep Kit for Illumina (NEB) following the manufacturer’s instructions and sequenced on Miseq (Illumina ...
-
bioRxiv - Molecular Biology 2019Quote: ... tRNA containing plasmids were linearized using restriction digestion and tRNAs were in vitro transcribed overnight using the HiScribe T7 transcription kit (NEB) per the manufacturer’s protocol ...
-
bioRxiv - Microbiology 2021Quote: ... The Y-adaptor was ligated to the ends of the fragments using the NEBNext Ultra II DNA Library Prep Kit for Illumina (New England BioLabs) according to the manufacturer’s instructions ...
-
bioRxiv - Genomics 2020Quote: ... The pbp2x 5’-end and 3’-end amplicons were spliced with the linearized pBBL740 amplicon using NEBuilder HiFi kit (New England Biolabs). The resultant spliced plasmid was transformed into parental strain MGAS27213-L601P and single cross-over transformants were selected by plating on THY agar with chloramphenicol 10ug/ml ...
-
bioRxiv - Physiology 2021Quote: ... ChIP-seq libraries were prepared from 1 to 5 ng eluted DNA using NEBNext Ultra II DNA library Prep Kit (New England BioLabs), with 12 cycles of library amplification ...
-
bioRxiv - Pharmacology and Toxicology 2020Quote: ... 1B) driving intestinal Pun-PGP-9 expression were assembled using the NEB HIFI DNA Assembly Kit (New England Biolabs Inc.) according to the manufacturer’s instructions into the supplied pUC19 vector linearized by SmaI (ThermoFisher ...
-
Regulation of skeletal muscle metabolism and contraction performance via teneurin-latrophilin actionbioRxiv - Physiology 2021Quote: ... The LPHN-1 and -3 genes were amplified by PCR and digested by T7 endonuclease using the EnGen Mutation Detection Kit (New England Biolabs) according to directions in combination with the custom primers that flank the appropriate CRISPR-targeting regions (Fig ...
-
bioRxiv - Pharmacology and Toxicology 2020Quote: ... RNA was subjected to OneStep qRT-PCR analysis using the Luna Universal One-Step RT-qPCR Kit (New England Biolabs) and a CFX96 Real-Time System ...
-
bioRxiv - Biochemistry 2021Quote: ... pET-28a(+)::aprt2-Ntag and pET-28a(+)::aprt2-Ctag were linearized by PCR (S2 Appendix) and cloned into the pPICZ vectors (pPICZ-Ntag and pPICZ-Ctag) using a HiFi DNA assembly kit (NEB). Assembled products were subsequently transformed into NEB 5-alpha competent Escherichia coli cells using heat-shock and incubated overnight at 37°C on LB agar plates containing kanamycin 50 μg mL-1 ...
-
bioRxiv - Developmental Biology 2021Quote: ... Synthesis of cDNA from the RNA subsamples was carried out with First Strand cDNA Synthesis Kit (ProtoScript® II; NEB) according to the manufacturer’s instructions ...
-
bioRxiv - Biochemistry 2021Quote: ... and used here as a template to obtain mutants C204S and W195D using the Q5 mutagenesis kit (New England Biolabs) and to subclone construct Arpin_CA (residues 194-226) ...
-
bioRxiv - Biochemistry 2021Quote: The Saccharomyces cerevisiae Mtr4ΔF was constructed by removing residues 667-813 and inserting a three-glycine linker using the Q5 Site-Directed Mutagenesis Kit (NEB). All Mtr4 proteins were recombinantly expressed in Escherichia coli BL21-CodonPlus (DE3)-RIPL (Stratagene ...
-
bioRxiv - Biochemistry 2021Quote: Site directed mutagenesis for Ser247Thr mutation on hMAT2A plasmid and Thr227Ser mutation on eMAT plasmid was carried out using Q5 Site-Directed Mutagenesis Kit (NEB) by following kit protocol and expressed ...
-
bioRxiv - Microbiology 2021Quote: ... PCR products were equally mixed and purified with Qiagen Gel Extraction kit before libraries for paired-end 250bp Illumina sequencing were prepared with NEBNext Ultra DNA Library Prep Kit (New England Biolabs).
-
bioRxiv - Microbiology 2021Quote: ... The PCR products were cloned into the plasmid backbones using the NEBuilder HiFi DNA Assembly Cloning Kit (New England Biolabs) following manufacturer’s instructions ...
-
bioRxiv - Molecular Biology 2021Quote: ... Plasmid #90097) after the plasmid digestion by BamHI and NotI with NEBuilder HiFi DNA Assembly Cloning Kit (New England Biolabs). The expression constructs were transformed into BL21 (DE3 ...
-
bioRxiv - Cell Biology 2022Quote: ... EIF2AK2 and EIF2S1 sequences were inserted into pET-His 1a vector using the Gibson Assembly Cloning Kit (New England Biolabs) as described by the manufacturer.
-
bioRxiv - Cell Biology 2021Quote: ... Approximately 50-300 ng of total RNA was used for cDNA synthesis with random primers (LunaScript SuperMix Kit, New England Biolabs), depending on the cells used ...
-
bioRxiv - Biochemistry 2021Quote: ... These DNA constructs were used as a template to in vitro transcribed RNA using T7 high yield RNA kit (New England Biolabs). The synthesized RNA was DNase treated (TURBODNase) ...
-
bioRxiv - Biochemistry 2021Quote: ... The NEBNext Ultra or Ultra II Directional RNA Library Prep Kit for Illumina with the mRNA magnetic isolation module (New England Biolabs) was used to prepare sequencing libraries from 1 μg of total RNA.
-
bioRxiv - Biochemistry 2021Quote: ... Purified DNA was resuspended in 10 mM Tris pH 8 and prepared for sequencing using the NEBNext Ultra II DNA library kit for Illumina (New England Biolabs). Paired-end sequencing with 75 cycles and a 6-cycle index read was performed on the Illumina NextSeq500 system.
-
bioRxiv - Synthetic Biology 2021Quote: ... The libraries for next generation sequencing (NGS) were prepared from 100 ng DNA samples using the NEBNext Ultra II DNA Library Prep kit (New England Biolabs) with AMPure XP purification beads (Beckman Coulter) ...
-
bioRxiv - Immunology 2021Quote: ... cDNA libraries were generated using NEBNext® Ultra™ II Directional (stranded) RNA Library Prep Kit for Illumina (NEB #E7760L). Ribosomal RNA was removed using NEBNext rRNA Depletion Kit (human ...
-
bioRxiv - Cancer Biology 2020Quote: ... 200ng of total RNA with RIN >9.5 were used to prepare library using NEBNext poly(A) mRNA Magnetic Isolation Module and the NEB Next Ultra II RNA library prep kits for Illumina (New Engand Biolabs). Qubit High Sensitivity DNA kit (Life Technologies ...
-
bioRxiv - Cell Biology 2019Quote: A construct expressing SURF4 and the Katushka2S fluorescent marker (PGK-SURF4-p2A-Katushka2S) was assembled with the NEBuilder HiFi DNA assembly cloning kit (NEB) using vector sequence derived from LV1-5 (Addgene #68411 ...
-
Satb2 acts as a gatekeeper for major developmental transitions during early vertebrate embryogenesisbioRxiv - Developmental Biology 2020Quote: ... was used as an input for library preparation and libraries were prepared using NEB ultra II DNA library prep kit (NEB). Sequencing reads (76 bp PE ...
-
bioRxiv - Evolutionary Biology 2021Quote: ... mRNA libraries were constructed using the kits NEBNext Poly(A) mRNA Magnetic Isolation Module and NEBNext UltraTM II Directional RNA Library Prep Kit for Illumina (New England BioLabs) following the manufacturer protocol ...
-
bioRxiv - Systems Biology 2021Quote: ... Fragment end repair and adaptor ligation was performed using an NEBNext Ultra II DNA Library Prep Kit (New England Biolabs) following the manufacturer’s protocol ...
-
bioRxiv - Molecular Biology 2021Quote: ... 16 μl of SSS reaction products were combined with 24 μl HiScribe T7 High Yield RNA Synthesis Kit (NEB, E2040S) reagent mix containing 4 μl T7 Buffer ...
-
bioRxiv - Genomics 2021Quote: Poly(A)+ sequencing libraries were generated from total RNA using the NEBNext Ultra II Directional RNA Library Prep Kit (New England Biolabs) according to the manufacturer’s instruction ...
-
bioRxiv - Biophysics 2020Quote: ... A Pol I construct containing an additional L361A mutation was generated from the WT construct by site-directed mutagenesis using a Q5 site-directed mutagenesis kit (NEB) according to manufacturer’s protocol ...
-
α-catenin links integrin adhesions to F-actin to regulate ECM mechanosensing and rigidity-dependencebioRxiv - Cell Biology 2021Quote: ... V796A mutations were inserted into the GFP-α-catenin plasmid using the Q5 Site-Directed Mutagenesis Kit (New England Biolabs).
-
bioRxiv - Pathology 2021Quote: Reverse-transcription polymerase chain reaction (RT-PCR) was performed using the Luna Universal Probe One-Step RT-qPCR kit (NEB). Two gene targets were used for SARS-CoV-2 RNA detection ...
-
bioRxiv - Neuroscience 2022Quote: ... Sheared DNA fragments was Subjected to Illumina paired-end DNA library using NEBNext ultra II DNA Library Prep Kit (E7645L, NEB). Preparation and PCR-amplified for three cycles and libraries size were selected with 0.55-1X magnetic beads (Ampure XP ...
-
bioRxiv - Biophysics 2021Quote: ... In vitro transcription on the DNA substrates was performed by using HiScribe T7 High Yield RNA synthesis kit (E2040S, New England BioLabs) for 120 min at 37 °C in the presence of 1 mM GTP ...
-
bioRxiv - Developmental Biology 2020Quote: ... cDNA synthesis was carried out using AMV First Strand cDNA Synthesis Kit following manufacturer’s instructions (New England BioLabs. Ipswich, MA). PCR amplification was performed using Expand High Fidelity PCR System (Roche ...