Labshake search
Citations for New England Biolabs :
6501 - 6550 of 9425 citations for Mouse Plectin PLEC ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2019Quote: ... The remaining 50 μL was used as input for one round of end repair and adapter ligation with NEBNext Ultra II DNA Library Preparation kit (NEB), according to manufacturer’s instructions ...
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bioRxiv - Genetics 2020Quote: ... and wobble mutations to make constructs resistant to shOTUD5#5 were introduced in this vector using the Q5 site-directed mutagenesis kit (E0554, NEB) following the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2019Quote: ... Most point mutants and wild-type variants were generated from this vector following the Q5 Site-Directed Mutagenesis Kit protocol (E0554, New England Biolabs) and primers 1-8 (Sup ...
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bioRxiv - Genomics 2019Quote: ... polyA+ RNA and polyA-RNA were then made into a strand specific library using the NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (NEB) according to the manufacturers protocol (utilizing a 15-minute fragmentation step ...
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bioRxiv - Molecular Biology 2019Quote: ChIP-seq libraries for both ChIP and input samples were prepared using the NEBNext Ultra DNA Library Prep Kit for Illumina (NEB), following the manufacturer’s guidelines and using NEBNext Multiplex Oligos ...
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bioRxiv - Physiology 2019Quote: ... The suicide delivery vector pEMG was isolated using the NEB Monarch Plasmid Miniprep Kit (New England Biolabs, Ipswich, MA, USA). The isolated plasmid was digested with restriction enzymes purchased from New England Biolabs (Ipswich ...
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bioRxiv - Synthetic Biology 2019Quote: ... Reagents and competent DH5a cells used for cloning were from the NEBuilder HiFi DNA Cloning Kit (Product E5520S New England Biolabs). Q5® High-Fidelity 2X Master Mix was used for long PCRs (Product M0492SNew England Biolabs 240 County Road Ipswich ...
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bioRxiv - Cell Biology 2019Quote: ... the pGL4.10-P plasmid was mutated by PCR using mutant primers and Q5 site-directed mutagenesis kit (New England Biolabs, E0554) according to the manufacturer’s instructions ...
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bioRxiv - Genomics 2019Quote: ... The assembly reaction of 1:5 vector:insert ratio was carried out for 1 hour at 50C using NEBuilder HIFI DNA assembly kit (New England Biolabs, NEB). Assembly products were electroporated into NEB Turbo Competent E.Coli (NEB ...
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bioRxiv - Genomics 2019Quote: ... The assembly reaction of 1:5 vector:insert ratio was carried out for 1 hour at 50C using NEBuilder HIFI DNA assembly kit (New England Biolabs, NEB). Assembly products were electroporated into NEB Turbo Competent E.Coli (NEB ...
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bioRxiv - Biophysics 2019Quote: ... v/v) and then prepared for Illumina sequencing with the NEBNext Ultra II DNA Library Prep Kit (New England BioLabs). The adaptor-ligated DNA was amplified for 10 cycles following the manufacturer’s protocol ...
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bioRxiv - Evolutionary Biology 2019Quote: ... We treated DNA extractions with 2 μl of RNAase A (ThermoScientific) and purified them with Monarch PCR & DNA Cleanup Kit (New England Biolabs). We assessed DNA quantity and quality with Qubit 3.0 (Thermofisher) ...
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bioRxiv - Developmental Biology 2019Quote: ... Library preparation was carried out using 0.5 μg of RNA with NEBNext Ultra RNA library Prep Kit for Illumina (New England BioLabs, E7530L). For RNA-seq from E7.5 embryos ...
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bioRxiv - Developmental Biology 2019Quote: ... and was used to prepare sequencing libraries with NEB Next Ultra RNA Library Prep Kit for Illumina (New England Biolabs). Briefly ...
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bioRxiv - Plant Biology 2020Quote: RNA-seq libraries were generated from 1µg of input RNA using NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (NEB, E7490) according to the manufacturer’s protocols ...
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bioRxiv - Microbiology 2019Quote: RNA was reverse-transcribed with random priming using the NEBNext Ultra Directional RNA Library Prep Kit for Illumina (New England Biolabs) with 5ul sample input ...
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bioRxiv - Synthetic Biology 2019Quote: ... The amplicon corresponding to a genomic fragment of 1240 bp was run on a gel and the DNA was excised and purified with Monarch DNA Gel Extraction Kit (NEB). DNA concentration and absorbance ratio were measured with a NanoDrop Spectrophotometer (ThermoScientific) ...
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Replication Timing and Transcription Identifies a Novel Fragility Signature Under Replication StressbioRxiv - Genetics 2019Quote: ... Sequencing libraries of BrdU-substituted DNA from early and late fractions were prepared by NEBNext Ultra DNA Library Prep Kit for Illumina (E7370; New England Biolabs). Sequencing was performed on an Illumina-HiSeq 2500 sequencing system by 50-bp single-end reads ...
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bioRxiv - Cell Biology 2019Quote: ... were derived from pSS393 by using the Q5 site-directed mutagenesis procedure (pSS396) or the NEBuilder assembly kit (New England Biolabs) (pSS397) ...
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bioRxiv - Microbiology 2019Quote: ... The first-round PCR products were converted into an Illumina-compatible deep sequencing library using the NEBNext Ultra DNA Library Prep Kit (NEB), and deep sequencing was carried out using Illumina’s NextSeq platform ...
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bioRxiv - Molecular Biology 2019Quote: ... rRNA-depleted RNA was used as a starting material for library construction using the NEBNext Ultra Directional RNA Library Prep Kit for Illumina (New England Biolabs). Two biological replicates per condition were sequenced on Illumina HiSeq 2500 instrument at Millard and Muriel Jacobs Genetics and Genomics Laboratory at Caltech yielding 15-20 million single-end 50-bp reads ...
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bioRxiv - Genomics 2019Quote: ... designed to be directly cloned into the BbsI or BsaI-digested and dephosphorylated AIO-GFP(Cas9) vector using the Quick Ligation Kit (NEB). The first and second sgRNA was cloned into the BbsI and BsaI sites ...
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bioRxiv - Biophysics 2019Quote: ... final products were separated from non-ligated fragments by electrophoresis using a 0.8-1.5% (m/V) agarose gel and extracted from the gel with the Monarch® DNA Gel Extraction Kit (NEB).
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bioRxiv - Genomics 2019Quote: ... These regions were mutagenized in order to introduce the de novo mutations of interest using the Q5 Site-Directed Mutagenesis kit (NEB) using non overlapping primers ...
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bioRxiv - Cancer Biology 2019Quote: ... All RNA-seq libraries were prepared using the NEBNext Poly(A) mRNA Magnetic Isolation Module followed by NEBNext Ultra Directional RNA Library Prep Kit for Illumina (both from NEB). Library quality was analyzed using Agilent BioAnalyzer 2100 (Agilent ...
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bioRxiv - Genomics 2019Quote: Illumina TruSeq Stranded mRNA and TruSeq Small RNA kits were used for mRNA-seq and small RNA-seq library preparations and the NEB Next Ultra ChIPseq Library Prep kit (New England Biolabs) was used for ChIP-seq library preparations ...
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bioRxiv - Genetics 2019Quote: ... RNA-seq libraries were then prepared from 200 ng of RNA using the NEBNext Ultra II Directional RNA-seq kit (NEB). To quantitate the consistency of spike-in cell mixing for each individual sample ...
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bioRxiv - Genomics 2019Quote: ... extracted RNA without rRNA depletion was directly subjected to library preparation with the NEBNext Small RNA Library Prep Set kit (New England Biolabs) and sequenced on a HiSeq2000 (Illumina ...
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bioRxiv - Synthetic Biology 2020Quote: ... and used as input for library preparation using NEBNext Ultra II DNA Library Prep Kit (New England Biolabs, cat. E7645S), according to manufacturer instructions.
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bioRxiv - Cancer Biology 2020Quote: ... DNA fragments were purified from the reaction using a Zymo DNA Clean & Concentrator-5 kit (in-house) and amplified with NEBNext High Fidelity PCR Mix (NEB). Library quality was assessed using a Fragment Analyzer system (Agilent) ...
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bioRxiv - Molecular Biology 2020Quote: ... prior to preparing cnRNA-seq libraries using the NEBNext Ultra (for Bap1fl/fl and PRC1CPM ESCs) or Ultra II (for PRC1CPM;Bap1fl/fl ESCs) Directional RNA Library Prep kit (NEB). To quantitate the consistency of spike-in cell mixing for each individual sample ...
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Combinations of maternal-specific repressive epigenetic marks in the endosperm control seed dormancybioRxiv - Molecular Biology 2020Quote: ... and cDNA libraries were constructed using the NEBNext® Ultra™ II RNA Library Prep Kit for Illumina® (NEB). Libraries were sequenced on Illumina NovaSeq platforms as paired-end through Novogene mRNA sequencing services ...
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bioRxiv - Molecular Biology 2020Quote: ... The sequence libraries were generated using the NEBNext Ultra II DNA Library Prep Kit for Illumina (E7645, New England Biolabs) following the manufacturer’s instructions ...
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bioRxiv - Synthetic Biology 2020Quote: ... Colony PCR was used to screen surviving transformants for the presence of the 520-522 insert using primers spanning the insert junctions and amplification with Taq 2X Master Mix PCR Kit (New England Biolabs), as described by the supplier ...
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bioRxiv - Genomics 2020Quote: ... following the manufacturer’s instructions for the kit NEBNext® Ultra™ II DNA (New England Biolabs Inc. Ipswich, MA, USA). The libraries were sequenced using an Illumina NextSeq500 system (s/n NB501764).
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bioRxiv - Developmental Biology 2020Quote: ... Sequencing libraries were made with the fragmented DNA using a NEBNext Ultra DNA Library Prep Kit for Illumina according to the manufacturer’s instructions (cat. #E7370, New England Biolabs, USA). Library quality inspection was established via a LabChip GX Touch (PerkinElmer ...
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bioRxiv - Biochemistry 2020Quote: ... lpg1621 and lpg1949 were amplified by PCR from genomic DNA (kind gift from A. Hamprecht, University Hospital of Cologne) using the Phusion High Fidelity Kit (New England Biolabs). JOSD2 and UCHL3 were amplified from HEK293-derived cDNA accordingly ...
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bioRxiv - Systems Biology 2019Quote: ... followed by library preparation using the NEBNext Poly(A) Magnetic Isolation Module and NEBNext Ultra RNA sequencing library prep kit for Illumina (NEB). At the time of RNA isolation for the clones ...
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bioRxiv - Developmental Biology 2020Quote: ... We previously generated a human LRP5-V5 plasmid [32] and we used the Q5 site-directed mutagenesis kit (New England Biolabs) with forward primer (CTCAATGGCACATCCCGG ...
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bioRxiv - Molecular Biology 2019Quote: ... DNase-treated samples were dissolved in 10 μl water and used for sequencing library preparation using the NEBNext Directional Ultra RNA kit and Indexing primers (E7530, and E7335, respectively, both NEB) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2019Quote: ... DNase-treated samples were dissolved in 10 μl water and used for library preparation using the NEBNext Ultra II Directional RNA Library Prep Kit and Indexing primers (E7760, and E7335, respectively, both NEB) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2019Quote: ... Purified ChIP DNA was used for library generation using the NEBNext Ultra II DNA Library Prep Kit for Illumina (NEB) following manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2020Quote: ... The sgRNA was constructed by annealing sense and anti-sense single stranded oligonucleotides containing 5’ Bsa1 restriction overhangs and was inserted into Bsa1 linearized pDR274 with the Quick Ligase Kit (NEB) (Table 1) ...
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bioRxiv - Cell Biology 2020Quote: ... The kinase-inactive mutant was generated by a two-step PCR method using the Phusion High-fidelity kit (New England Biolabs). In the first step ...
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bioRxiv - Cell Biology 2020Quote: ... The AP-3 sorting mutant was regenerated in MigR1-PI4KIIα-GFP using the Q5 site-directed mutagenesis kit (New England Biolabs) following manufacturer’s instructions ...
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bioRxiv - Physiology 2020Quote: ... RNA with RIN>8 was used to prepare transcriptomic libraries using the NEBNext Ultra RNA library prep kit (New England Biolabs). High throughput RNA-Sequencing was performed at the NIDDK Genomic Core Facility (NIH ...
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bioRxiv - Synthetic Biology 2019Quote: ... Mutagenesis was performed using the partial overlapping primer design method or using a Q5 Site-Directed Mutagenesis Kit (New England Biolabs).
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bioRxiv - Genomics 2019Quote: ... We prepared D3839 libraries using the NEBNext Ultra II DNA Library Prep Kit for Illumina (New England Biolabs: E7645S/L) according to the manufacturer’s protocol ...
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bioRxiv - Genomics 2020Quote: ... ethanol purified used to produce barcoded RNA sequencing libraries using the NEBNext Ultra II Directional RNA Library Prep kit (New England Biolabs). Libraries were sequenced on Illumina NextSeq 500.
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bioRxiv - Genomics 2020Quote: ... and used to produce barcoded RNA sequencing libraries using the NEBNext Ultra II Directional RNA Library Prep kit (New England Biolabs). Libraries were sequenced on Illumina NextSeq 500.