Labshake search
Citations for New England Biolabs :
6451 - 6500 of 9425 citations for Mouse Plectin PLEC ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2022Quote: Samples included 1) cDNA generated from >200nt RNA extracted previously from the 12h embryo sample (LunaScript RT Mastermix Kit, New England Biolabs). 2 ...
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bioRxiv - Developmental Biology 2022Quote: ... Linearized DNA was used as a template and RNA synthesis was performed using the HiScribe SP6 RNA Synthesis Kit (NEB) according to manufacturer’s instructions ...
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bioRxiv - Cell Biology 2022Quote: ... The purified DNA template was subjected to in vitro transcription by T7 RNA polymerase using the HiScribe T7 High Yield RNA Synthesis Kit (New England Biolabs). After being treated with DNase I (Takara Bio) ...
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bioRxiv - Microbiology 2022Quote: Plasmids were constructed by amplifying the sequence of interest using the MG1655 genomic DNA as template and inserting it into the desired vector using either the Gibson Assembly Cloning Kit (New England Biolabs) or by digestion with the indicated restriction enzymes followed by ligation using T4 DNA ligase (New England Biolabs) ...
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bioRxiv - Microbiology 2022Quote: ... The coding sequences of uvGFP and mCherry were then PCR amplified and cloned between the KpnI site using the NEBuilder kit (New England Biolabs). The sequences of LM4 ...
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bioRxiv - Microbiology 2022Quote: ... DNA libraries were prepared from extracted gDNA using the NEBNext Ultra II FS DNA Library Prep Kit for Illumina (NEB). DNA was purified and size selected using Ampure XP beads (Beckman Coulter ...
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bioRxiv - Microbiology 2022Quote: ... Designed fragments were PCR-amplified from purified phage A006 or synthetic DNA to yield a total of six DNA fragments per phage genome followed by Gibson assembly at 50°C for 1 h in a total reaction volume of 20 µl (NEBuilder HiFi DNA Assembly Cloning Kit, New England Biolabs). Assembly reactions were carried out with purified DNA fragments to yield synthetic genomes ...
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bioRxiv - Genomics 2022Quote: ... The pooled oligos were next assembled into the NotI-HF-digested plasmid pSAC200 using NEBuilder HiFi DNA Assembly Cloning Kit (New England Biolabs) and transformed via electroporation into Endura Electrocompetent E ...
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bioRxiv - Microbiology 2022Quote: The copy number of viral RNAs were titrated by qRT-PCR using Luna Universal Probe One-Step RT-qPCR Kit (NEB) and LightCycler 96 instrument (Roche) ...
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bioRxiv - Genomics 2022Quote: Real-time PCR (RT-PCR) amplification of RNA was performed following the Luna Universal One-Step RT-qPCR kit (New England Biolabs). A CFX 96 Touch Real-Time PCR Thermocycler/ Detection System (Bio-Rad ...
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bioRxiv - Biochemistry 2022Quote: Site-directed mutagenesis to generate alanine or tryptophan substitutions at H2 positions in NIP constructs was done by using the mutagenesis primers listed in Table SI and the Q5 Site-Directed Mutagenesis Kit (NEB) as described previously (50) ...
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bioRxiv - Genomics 2022Quote: ... DNA libraries were prepared from 5 ng of total genomic DNA using the NEBNext Ultra II FS DNA Library Kit for Illumina (New England Biolabs) following manufacturers’ protocols ...
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bioRxiv - Evolutionary Biology 2022Quote: ... Dual-indexed mNGS library preparations for the samples were miniaturized and performed in 384-well format with NEBNext Ultra II RNAseq library preparation kit (New England Biolabs) reagents ...
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bioRxiv - Developmental Biology 2022Quote: miRNAs and total mRNA were extracted from a pool of 10 zebrafish hindbrains using the miRVana miRNA Isolation kit and gDNA removed using DNase1 (NEB). Reverse transcription was performed with either TaqMan MicroRNA Reverse Transcription kit (Applied Biosystems ...
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bioRxiv - Immunology 2022Quote: ... K986P and V987P mutations were introduced into the wildtype backbone using Q5 Site-Directed Mutagenesis Kit (NEB, catalog no. E0554S). Transiently transfected cells were stained the following day with mAbs conjugated using DyLight® 650 Conjugation Kit (Fast ...
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bioRxiv - Immunology 2022Quote: ... and nCoV_IP4-14084Probe(+) TCATACAAACCACGCCAGG [5’]Hex [3’]BHQ-1 19) and the Luna Universal Probe One-Step RT-qPCR Kit (NEB). Serial dilutions of a titrated viral stock were analyzed simultaneously to express viral loads as eqPFU per gram of tissue.
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bioRxiv - Microbiology 2022Quote: ... Mutations were introduced into the phCMV-Spike plasmids using the mutagenic primers and the Q5 site directed mutagenesis kit (NEB). In addition ...
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bioRxiv - Cancer Biology 2022Quote: ... Sequencing libraries were generated by the Genome Analysis and Technology Core at the University of Virginia using oligo dT-purified mRNA from 500ng of total RNA and the NEB Next Ultra RNA library preparation kit (New England Biolabs), and 50-bp single-end sequencing was performed on the Illumina NextSeq500 platform (Illumina) ...
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bioRxiv - Cell Biology 2022Quote: ... an 887bp portion of mtDNA containing nd-1 was amplified by PCR and TA-cloned into pMiniT2.0 using the NEB PCR cloning kit (NEB E1202S). Purified plasmid was linearized with BamHI-HF (NEB 3136) ...
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bioRxiv - Cancer Biology 2022Quote: ... RNA was converted to a sequencing ready library using the NEBNext Ultra II Directional RNA library kit (New England Biolabs). The cDNA libraries were quantitated using qPCR in a Roche LightCycler 480 with the Kapa Biosystems kit for Illumina library quantitation (Kapa Biosystems) ...
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bioRxiv - Developmental Biology 2022Quote: ... 30 ng of total RNA was used for next-generation library preparation under the guidelines of the NEBNext Ultra DNA Library Prep Kit for Illumina (E7370; NEB). The libraries were loaded for 2 × 150 bp pair-end sequencing using Illumina Hiseq 2500 ...
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bioRxiv - Genetics 2022Quote: ... The resulting gDNA fragments were prepared for sequencing using NEBNext Ultra II DNA Library Prep Kit for Illumina (New England Biolabs). Bead-based size selection was used to enrich for 200 bp fragments prior to a 21-cycle splinkerette PCR utilizing a custom Tn5-enriching forward primer (oAM055 ...
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bioRxiv - Cell Biology 2022Quote: ... Ravikant Ranjan) was digested using BglII and EcoRV and TgVPS15 cDNA was ligated in it using Gibson assembly kit (NEB) to obtain pCT-TgVPS15-iCWT ...
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bioRxiv - Cell Biology 2022Quote: ... pIREShyg2-HA-hAdipoR2-cMYC(H348A) construct was generated using PCR-based mutagenesis (Q5-site-directed mutagenesis kit, New England Biolabs) with the following primers ...
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bioRxiv - Biochemistry 2022Quote: ... Purified RNAs were used to make the RNA-seq libraries using NEBNext Ultra Directional RNA Library Prep Kit for Illumina (E7760, NEB), followed by RNA-seq ...
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bioRxiv - Genomics 2022Quote: ... we prepared libraries for adaptive nanopore sequencing using the Ligation Sequencing Kit (Oxford Nanopore Technologies, ONT) and the NEBNext Companion Module for ONT Ligation Sequencing (New England Biolabs). 3 μg gDNA was used as input for these libraries ...
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bioRxiv - Genomics 2022Quote: ... After 25 cycles of amplification the product was purified with the Monarch PCR&DNA Cleanup kit (New England BioLabs T1030), and eluted with 20 μl of ddH2O ...
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bioRxiv - Molecular Biology 2022Quote: ... 40 μL of each digest product were then ran on a 3% agarose gel and the appropriate band was gel extracted using a kit (NEB) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2022Quote: ... 40 μL of digest product was then ran on a 3% agarose gel and the appropriate band was gel extracted using a kit (NEB) according to the manufacturer’s instructions ...
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bioRxiv - Systems Biology 2022Quote: Paired-end libraries were prepared according to the manufacturer’s recommendations using NEBNext Ultra II DNA Library Prep Kit (New England Biolabs, USA). The libraries were indexed with NEBNext Multiplex Oligos kit for Illumina (96 Index Primers ...
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bioRxiv - Developmental Biology 2022Quote: ... DNA libraries were prepared for each individual titration point using the NEBNext Ultra II DNA Library Prep Kit for Illumina (E7370, New England Biolabs) and barcoded using NEBNext Multiplex Oligos for Illumina (Index Primers Set 1 & 2 ...
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bioRxiv - Developmental Biology 2022Quote: ... at 4°C for up to one month until used for purification of total RNA with the Monarch Total RNA Miniprep Kit (NEB). Five larvae were pooled for each RNA purification and homogenized in DNA/RNA protection buffer with a glass homogenizer prior to proteinase K digestion ...
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bioRxiv - Developmental Biology 2022Quote: ... and the reaction was purified with the Monarch RNA Cleanup Kit (500 micrograms) with elution in 50 µl nuclease-free water (NEB). The quality of the gRNA prep was confirmed by agarose gel electrophoresis and quantified using a NanoDrop One (Thermo Fisher).
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bioRxiv - Molecular Biology 2019Quote: ... quantified by qPCR using the NEBNext® Library Quant Kit for Illumina® (New England Biolabs® Inc., MA, USA), and fragment size (330 bp ...
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bioRxiv - Cell Biology 2019Quote: ... we introduced flanking FRT sites 104bp upstream of the transcription start site and 99bp downstream of the end of the 3’UTR using a Q5 Site-Directed Mutagenesis kit (New England Biolabs) and the following primers:
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bioRxiv - Molecular Biology 2019Quote: Sequencing libraries were prepared using NEBNext® Fast DNA Library Prep Set for Ion Torrent(tm) Kit (New England Biolabs) according to the manufacturer’s instructions ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... Fragmentation of RNA followed by reverse transcription and second strand cDNA synthesis was performed using NEBNext Ultra RNA Library Prep Kit for Illumina (cat. No. E7530, NEB). The resulting double-stranded cDNA’s were further processed to DNA sequencing libraries using ThruPLEX DNA-seq 12S Kit (Rubicon Genomics ...
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bioRxiv - Physiology 2019Quote: ... RNA library was prepared using the NEBNext® Ultra II Directional RNA Library Prep Kit for Illumina (New England Biolabs). Sequencing is performed on Illumina NextSeq 550 System ...
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bioRxiv - Biochemistry 2019Quote: ... TruSeq-barcoded RNAseq libraries were generated from half of the rRNA-subtracted samples with the NEBNext Ultra II Directional RNA Library Prep Kit (New England Biolabs). Each library was quantified with a Qubit 2.0 (dsDNA HS kit ...
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bioRxiv - Biophysics 2019Quote: ... Site directed mutagenesis of the HEV510-696 was performed using primers detailed in Table 1 and the Q5 Site-directed Mutagenesis Kit (NEB).
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bioRxiv - Molecular Biology 2019Quote: ... Half of the eluted RNA was used for strand-specific RIP-Seq library preparation using the NEBNext Ultra II Directional RNA Library Prep Kit (New England Biolabs).
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bioRxiv - Genetics 2019Quote: Illumina libraries with TruSeq barcode adapters were prepared using NEBNext Ultra II FS DNA Library Prep Kit for Illumina (NEB) on the 1/4 scale of the manufacturer-suggested protocol ...
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bioRxiv - Animal Behavior and Cognition 2020Quote: ... Sequencing libraries were generated using NEBNext® Ultra™ RNA Library Prep Kit for Illumina® (NEB, Ipswich, MA, USA) following manufacturer’s recommendations and index codes were added to attribute sequences to each sample.
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bioRxiv - Microbiology 2019Quote: ... end prepped with dA tailing Kit (NEBNext® Ultra™ End Repair/dA-Tailing Module, New England Biolabs, MA, USA), bead purified (1:1 ...
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bioRxiv - Molecular Biology 2019Quote: Illumina RNA sequencing libraries from purified mRNA were prepared and sequenced by the Centre for Genomic Research at University of Liverpool using the NEBNext Ultra Directional RNA Library Prep Kit for Illumina (New England Biolabs). Paired-end sequencing with a read length of 150 bp was carried out on an Illumina HiSeq 4000 ...
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bioRxiv - Genetics 2019Quote: ... The donor sequence was generated by subcloning 596 bp upstream of the hrpk-1 stop codon and 600 bp downstream of hrpk-1 stop codon into the pDD282 vector [45] using Hi Fi assembly kit (NEB). The hrpk-1 stop codon was eliminated from the donor sequence to allow in frame GFP tag addition ...
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bioRxiv - Plant Biology 2019Quote: Approximately 1 ug of RNA was used as input to the NEB Ultra II RNA sequencing library kit for illumina (New England Biolabs, NEB catalogue number ...
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bioRxiv - Plant Biology 2019Quote: Approximately 1 ug of RNA was used as input to the NEB Ultra II RNA sequencing library kit for illumina (New England Biolabs, NEB catalogue number ...
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bioRxiv - Molecular Biology 2019Quote: ... Size selection was performed by running samples on a 1.8% LMT agarose gel at 80 V for 40 minutes at room temperature and gel extracting the mononucleosome band with a Monarch Gel Extraction Kit (T1020S, New England Biolabs). Note that of the samples digested with 10 ...
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bioRxiv - Genetics 2019Quote: ... The amplicons were gel-purified on a 4% agarose gel and assembled with the cut Lenti-gRNA-Puro plasmid described above using an NEBuilder HiFi DNA Assembly kit (NEB). After incubation at 50 °C for 1 h ...