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Citations for New England Biolabs :
6351 - 6400 of 9547 citations for QuantiChrom Phosphate Assay Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2020Quote: ... Sequencing libraries were constructed with the NEBNext® Ultra™ II DNA Library Prep kit (New England Biolabs, MA, USA), and sequenced by Illumina-MiSeq at the Pittsburgh Bacteriophage Institute to an approximate shotgun coverage of 3538x ...
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bioRxiv - Plant Biology 2020Quote: ... Size-selected DNA fragments were used to prepare eight MNase sequencing libraries with the NEBNext Ultra DNA Library Prep Kit for Illumina (NEB), following manufacturer’s instructions (2 biological replicates x 2 MNase concentrations x 2 tissues) ...
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bioRxiv - Plant Biology 2020Quote: Sequencing libraries were generated from poly(A)-enriched RNA using the NEBNext Ultra II Directional RNA Library Prep kit (New England Biolabs) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: ... Equal amounts of RNA (~50 ng) were used for preparation of cDNA libraries with the NEBNext Multiplex Small RNA Library Prep kit for Illumina (NEB) with some minor changes to the manufacturers’ instructions ...
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bioRxiv - Systems Biology 2021Quote: ... using the NEBNext Poly(A) mRNA Magnetic Isolation Module and NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (New England Biolabs (NEB), Ipswich ...
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bioRxiv - Microbiology 2020Quote: ... these long gene sequences containing enzyme sites firstly was amplified by common PCR by Q5 high-fidelity PCR kit (Cat: #M0491, NEB), using primer F and R pairs of S or N ...
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bioRxiv - Systems Biology 2020Quote: ... Library preparation was performed using the NEBNext®Ultra™ II DNA Library Prep Kit for Illumina (New England Biolabs), and library quantification was performed using the KAPA library quantification kit (Kapa Biosystems) ...
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bioRxiv - Systems Biology 2020Quote: The extracted plasmids were amplified using “lib-seq” primers in the Supplementary Table 6 for 10 cycles using Phusion PCR kit (NEB). Each 50 μL PCR reaction consisted of 6 μL of plasmid library ...
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bioRxiv - Systems Biology 2020Quote: ... We neutralized the tagmentation activity of Enzyme 1 by immediately cleaning the reaction with the Monarch PCR & DNA Cleanup Kit (NEB) according to the manufacturer’s protocol ...
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bioRxiv - Systems Biology 2020Quote: RNA-seq libraries were prepared using 200 ng of total RNA and the NEBNext rRNA Depletion Kit (NEB, Cat: E6310X), per manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: Viral RNA genome for both WT ZIKV and the X1 mutant was in vitro transcribed from ligated plasmid DNA using a HiScribe T7 ARCA mRNA kit (NEB). Approximately 40 μg of the resulting mRNAs were transfected into Vero cells using MessengerMAX lipofectamine transfection reagent (Invitrogen) ...
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bioRxiv - Plant Biology 2021Quote: ... and the purified mRNA was used to build sequencing libraries using the Ultra II Directional RNA library Prep Kit for Illumina (NEB). Single end sequencing of 76 bp were performed on NextSeq instrument (Illumina) ...
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bioRxiv - Microbiology 2020Quote: RNA samples were confirmed to be DNA-free by conducting test PCRs on the 16S rRNA gene and converted to cDNA using the LunaScript RT SuperMix Kit (NEB) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: ... pLenti-puro construct expressing N-terminal HA-tagged human SAMHD1 was described previously8 and was used as template to generate mutants using the Q5® Site-Directed Mutagenesis Kit according to the manufacturer’s instructions (NEB). To obtain the SAMHD1ΔC-SUMO fusion proteins ...
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bioRxiv - Microbiology 2020Quote: ... 1 μl of the diluted cDNA was used in 10 μl reaction mixture of Q5 Hot START DNA Polymerase kit (NEB) (2 μl of 5X buffer ...
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bioRxiv - Neuroscience 2020Quote: ... mutations were inserted into the open reading frame of Vesicular stomatitis virus glycoprotein VSV-G using the Q5 SDM kit (NEB). Myc epitope-tagged SARS-CoV-2 (2019-nCoV ...
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bioRxiv - Microbiology 2020Quote: ... column purified (QIAquick PCR Purification kit) and ligated with restriction enzyme cut-pHH21 using T4-ligase (New England Biolabs, NEB). Ligation products were transformed into DH5a (NEB ...
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bioRxiv - Microbiology 2020Quote: ... column purified (QIAquick PCR Purification kit) and ligated with restriction enzyme cut-pHH21 using T4-ligase (New England Biolabs, NEB). Ligation products were transformed into DH5a (NEB ...
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bioRxiv - Microbiology 2021Quote: ... the codon optimized RBD was PCR amplified and cloned into the bicistronic pDZ rescue plasmid system for A/Puerto Rico/8/1934 using the NEBuider HiFi DNA assembly kit (NEB). Specifically ...
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bioRxiv - Genomics 2020Quote: ... The first step involved incubation of total DNA with proteinA-MBD2-Fc mixture (NEBNext Microbiome DNA Enrichment Kit, New England BioLabs, Microbial enrichment kit ...
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bioRxiv - Genomics 2020Quote: ... Samples were prepared for Illumina sequencing with NEBNext® Ultra™ II RNA Library Prep Kit for Illumina (NEB #E7775) accompanied by NEBNext Poly(A ...
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bioRxiv - Genomics 2021Quote: ... C and 5mC deamination reaction was performed using the APOBEC3A enzyme (NEBNext® Enzymatic Methyl-seq Kit, New England Biolabs) with the following ramping conditions ...
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bioRxiv - Genetics 2021Quote: ... Repair templates were generated by assembling homology arms (450-bp gBlocks synthesized by IDT) and the selection cassette using the NEBuilder Hifi DNA Assembly Kit (New England Biolabs).
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bioRxiv - Microbiology 2021Quote: ... cDNA was amplified and indexed using the PCR Barcoding Kit (Oxford Nanopore Technologies) and LongAmp Taq 2X master mix (New England Biolabs). The resulting cDNA libraries were sequenced on a MinION (MIN101B ...
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bioRxiv - Microbiology 2021Quote: ... and sequencing libraries were generated using the rRNA-depleted RNA samples using NEBNext Ultra Directional RNA Library Prep Kit for Illumina (New England BioLabs). Briefly ...
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bioRxiv - Microbiology 2021Quote: ... pcineo NS1 single site mutants in the full-length construct were generated by using the Q5 site-directed mutagenesis kit (New England BioLabs), following the manufacturer recommendations ...
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Lessons from the meiotic recombination landscape of the ZMM deficient budding yeast Lachancea waltiibioRxiv - Genetics 2021Quote: ... DNA libraries were prepared from 5 ng of total genomic DNA using the NEBNext Ultra II FS DNA Library kit for Illumina (New England Biolabs). All volumes specified in the manufacturer’s protocol were divided by four ...
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bioRxiv - Microbiology 2021Quote: ... was digested with NotI and NheI and the mgrB PCR product was cloned using the NEBuilder HiFi DNA assembly kit (NEB), followed by transformation into the E ...
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bioRxiv - Immunology 2021Quote: ... SARS-CoV-2 RNA levels were quantified with 250 ng of RNA inputs using the Luna Universal Probe One-Step RT-qPCR Kit (New England Biolabs), using real-time RT-PCR primer/probe sets 2019-nCoV_N1 (CDCN1 ...
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bioRxiv - Immunology 2021Quote: ... pSFG-SARS-CoV-2 S D614G was cloned from pSFG-SARS-CoV-2 S plasmid using the Q5 Site-Directed Mutagenesis Kit (New England BioLabs). 293T cells were transfected with 3.75 μg pSFG-SARS-CoV-2 S or pSFG-SARS-CoV-2 S D614G ...
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bioRxiv - Immunology 2021Quote: ... Point mutations and deletions of cGAS were constructed using a Q5® Site-Directed Mutagenesis Kit (New England Biolabs, # E0554S).
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bioRxiv - Immunology 2021Quote: ... The resultant DNA was cloned into a pUC19 plasmid and in vitro transcribed using a HiScribe T7 High Yield RNA synthesis kit (New England Biolabs). After DNAseI digestion and clean-up with a TURBO DNA-free kit (Thermo-Fisher) ...
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bioRxiv - Immunology 2021Quote: ... the pCMV3-SΔ19 insert was initially digested with KpnI and blunt polished using Phusion Taq polymerase followed by a DNA cleanup using the Monarch PCR cleanup kit (NEB) and a second digest was done using NotI ...
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bioRxiv - Neuroscience 2020Quote: ... Sequencing libraries were created using the NEBNext Ultra II DNA Library Prep kit for Illumina sequencing (New England Biolabs E7645L). All cDNA and library clean-up was done using SPRIselect beads (Beckman Coulter B23317).
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bioRxiv - Genomics 2021Quote: ... A reporter containing the alternate allele was generated through SDM using the Q5 Site-Directed Mutagenesis kit (New England Biolabs). The primer sequences used were as follows:
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bioRxiv - Genomics 2021Quote: ... Libraries were prepared using NEBNext Poly(A) mRNA magnetic isolation module and NEB Ultra Directional RNA Library kit for Illumina (New England Biolabs).
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bioRxiv - Microbiology 2020Quote: ... A bacteria-codon optimized gBlock for the truncated protein was produced by IDT DNA Technologies and cloned into a pET28a bacterial expression with a C-terminal 6xHis tag using the NEBuilder Assembly Kit (New England Biolabs). Recombinant protein was expressed in BL21(DE3 ...
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bioRxiv - Microbiology 2020Quote: ... The LPMO and CBH1 amplicons for detection were radio-labelled with [α-32P] dCTP using the NEBlot Kit (NEB, USA) according to the manufacturer’s instructions and used as a probe to determine the copy number of T-DNA integrations in all the transformants.
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bioRxiv - Microbiology 2020Quote: DNA was extracted from high-titer phage lysates and sequencing libraries were prepared using the Ultra II FS Kit (New England Biolabs) or for ARB14 and ARB25 ...
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bioRxiv - Molecular Biology 2020Quote: Many of the reagents used in 5XP-seq are included in the NEBNext Multiplex Small RNA Library prep kit for Illumina (New England Biolabs), and are referred to as ‘NEB-kit’ below ...
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bioRxiv - Microbiology 2021Quote: ... VN173 fused to NiV-M was generated by exchanging Ub from the previously described VN173-Ub (Pentecost et al., 2015) for NiV-M using the Gibson Assembly Cloning Kit (New England BioLabs). MeV-M BiFC constructs were made by exchanging NiV-M fused to VN173 or VC155 for MeV-M using the Gibson Assembly Cloning Kit (New England BioLabs) ...
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bioRxiv - Microbiology 2021Quote: ... All mutant plasmids were generated using the Q5 site-directed mutagenesis kit using PAGE-purified mutagenesis primers designed using the online NEBase Changer primer design tool (New England BioLabs). The mCherry plasmid was created by cloning mCherry into the multiple cloning site of pTriEx-3 (Novagen ...
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bioRxiv - Microbiology 2021Quote: ... MeV-M BiFC constructs were made by exchanging NiV-M fused to VN173 or VC155 for MeV-M using the Gibson Assembly Cloning Kit (New England BioLabs). All mutant plasmids were generated using the Q5 site-directed mutagenesis kit using PAGE-purified mutagenesis primers designed using the online NEBase Changer primer design tool (New England BioLabs) ...
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bioRxiv - Microbiology 2021Quote: ... Genomic libraries were constructed and barcoded using the NEBNext Ultra DNA Library Prep kit (New England Biolabs, Ipswich, MA, USA) per manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: ... were cloned into the pEX18.AP vector (with ampicillin resistance as a selectable marker) using the Gibson Assembly Cloning Kit (New England Biolabs) per the manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: ... PCR was carried out in 384-well plates using the Luna Universal Probe One-Step RT-qPCR Kit (New England Biolabs) with SARS-CoV-2 NP-specific primers (Forward 5’-TAA TCA GAC AAG GAA CTG ATT A-3’ ...
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bioRxiv - Neuroscience 2019Quote: ... The R1320P mutation was created in a wild-type cDNA using the Q5 Site-Directed Mutagenesis Kit (New England Biolabs). Wild-type and R1320P mutant dNf1 were then subcloned into the pUAST-attB vector with an in-frame C-terminal fusion with eGFP cDNA ...
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bioRxiv - Microbiology 2021Quote: ... and barcoded using an Native Barcoding Expansion 96 kit (Oxford Nanopore Technologies) and a Blunt/TA Ligation Master Mix (New England Biolabs). Barcoded samples (96 ...
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bioRxiv - Microbiology 2021Quote: ... 4μL purified RNA were subsequently used for RT-qPCR reaction using the Luna Universal One-Step RT-qPCR kit (New England Biolabs) and 0.4μM of each primer ...
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bioRxiv - Microbiology 2021Quote: ... Brunello and Vervet DNA samples were amplified in ten 100 μl reactions using the NEBNext High-Fidelity 2X Master Mix Kit (New England Biolabs), 0.5 μM of forward and reverse primers and 10 μg of DNA template per reaction with the following program ...