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Citations for New England Biolabs :
6151 - 6200 of 9501 citations for FabFc ZAP rat Antibody Internalization Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2022Quote: ... Second strand cDNA synthesis was performed with 1X SSS buffer and SSS enzyme (NebNext mRNA second strand synthesis kit NEB; thermocycler program ...
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bioRxiv - Evolutionary Biology 2022Quote: ... DNA was extracted with a Sodium Dodecyl Sulfate (SDS) protocol from whole bodies and sequencing libraries were built with NEBNext DNA Library Prep Kits (New England Biolabs) by Novogene (Hong Kong).
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bioRxiv - Evolutionary Biology 2022Quote: All constructs encoding for the protein fragments that were used as the tagless analyte in SPR experiments were produced using the IMPACT protein purification kit (NEB) following the manufacturer’s guidelines for cloning and refolding from denaturing conditions ...
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bioRxiv - Immunology 2022Quote: ... the purification of PolyA containing mRNA molecules using poly-T oligo attached magnetic beads from 100ng total RNA (with the Magnetic mRNA Isolation Kit from NEB), a fragmentation using divalent cations under elevated temperature to obtain approximately 300bp pieces ...
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bioRxiv - Immunology 2022Quote: ... was amplified from the synthetic S gene and cloned into the EcoRI and HindIII digested pXC17.4 CHO expression vector with a secretion signal sequence using NEBuilder HiFi DNA assembly kit (New England Biolabs, E5520). Similarly ...
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bioRxiv - Genomics 2020Quote: Synthetic SARS-CoV-2 RNA templates were serially diluted and amplified by RT-LAMP using the WarmStart LAMP Kit (NEB). LAMP primers were added to a final concentration of 0.2µM for F3 and B3 ...
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bioRxiv - Genomics 2020Quote: Small RNA sequencing libraries were generated using the NEBNext Small RNA library Prep kit and NEBNext multiplex oligos for Illumina according to the manufacturer’s instructions (New England Biolabs, E7300). The final small RNA libraries were purified from 6% PAGE gel ...
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bioRxiv - Genomics 2020Quote: ... In vitro transcription was performed using HiScribe T7 high yield RNA synthesis kit according to the manufacturer’s protocol (NEB, E2040S). In vitro synthesized RNAs was treated with 10U RNAse-free DNAse I (ThermoFisher Scientific ...
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bioRxiv - Genomics 2020Quote: ... using 6 µg input 3C DNA as previously described8 or using NEBNext Ultra II DNA Library Prep Kit for Illumina (New England Biolabs). When using the Ultra II kit 3 µg 3C material was sonicated to 200 bp as previously described8 ...
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bioRxiv - Genomics 2020Quote: The lentiviral vector for expressing TIR1 (Lentiv4-EFsp-Puro-2A-TIR1-9Myc) was constructed using PCR and Gibson Assembly Cloning kit (New England Biolabs). The backbone was modified from lentiCRISPR v259 (a gift from Feng Zhang ...
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bioRxiv - Genomics 2020Quote: PCR3 was performed by subtracting 2-3 cycles from the Ct and using 2.5 uL of a mix of distinct indexed primers from the NEBNext Dual Index Kit for Illumina (New England Biolabs) using 22.5 uL PCR2 product in a 50 uL reaction volume (Q5 UltraII mastermix with EvaGreen (Biotium) ...
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bioRxiv - Genomics 2020Quote: ... High quality RNA was subjected to library preparation using NEBNext Ultra™ RNA Library Prep Kit for Illumina (NEB, USA). The libraries were sequenced on a HiSeq X Ten sequencer (Illumina) ...
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bioRxiv - Microbiology 2020Quote: ... Genes with internal restriction sites for any of these two enzymes were subcloned into the same vector using the HIFI DNA Assembly kit (New England Biolabs) following the manufacturer’s protocol ...
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bioRxiv - Genomics 2020Quote: ... and Illumina-sequencer-compatible libraries were constructed by using an NEBNext Ultra II DNA Library Prep Kit (New England Biolabs).
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bioRxiv - Genomics 2020Quote: The immunoprecipitated sample and the input were subjected to Nextera library preparation as described by the manufacturer (NEBNext Ultra II DNA library Prep Kit for Illumina, NEB). The quality and size of libraries and chromatin shearing fragments were checked by Agilent Bioanalyzer using Agilent DNA High Sensitivity Chips (Agilent Technologies) ...
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bioRxiv - Genomics 2020Quote: ... end-repaired material (quantified by Nanodrop measurement) was ligated to Illumina adaptors supplied in the NEB DNA-seq kit (NEB cat ...
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bioRxiv - Molecular Biology 2021Quote: ... Libraries for cChIPseq and Native cChIPseq were prepared from 5-10ng of ChIP and corresponding input DNA samples using NEBNext Ultra II DNA Library Prep Kit for Illumina (New England Biolabs), following manufacturer’s guidelines ...
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bioRxiv - Molecular Biology 2021Quote: ... Two reactions of 1-5μg DNA each were adaptor-ligated and indexed using NEBNext Ultra II DNA Library Prep Kit for Illumina (New England Biolabs) and NEBNext Multiplex Oligos for Illumina Primer sets 1 and 2 (New England Biolabs) ...
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bioRxiv - Molecular Biology 2021Quote: Sequencing libraries were prepared using the NEBNext® Ultra™ II DNA Library Prep Kit for Illumina (NEB Cat. E7645S). For HA-Rap1 ChIP ...
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bioRxiv - Molecular Biology 2021Quote: Plasmid mutagenesis to create SARS-CoV-2 mutant genes was achieved using the Q5® Site-Directed Mutagenesis (SDM) Kit according to the manufacturer’s instructions (NEB) or by using Gibson assembly with mutations introduced into the complementary overhang regions of the primer sequences ...
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bioRxiv - Molecular Biology 2019Quote: ... Library preparation was done according to the manufacturer’s instructions with NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (NEB, E7760) and analyzed with 2100 Bioanalyzer with DNA 1000 kit (Agilent) ...
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bioRxiv - Genomics 2020Quote: ... and used to produce barcoded RNA sequencing libraries using the NEBNext Ultra II Directional RNA Library Prep kit (New England Biolabs). Libraries were sequenced on Illumina NextSeq 500 ...
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bioRxiv - Genetics 2019Quote: ... Libraries were quantified using a Qubit fluorometer before an 18-cycle PCR amplification on bar-coded fragments with Phusion high-fidelity PCR Kit (New England Biolabs). The PCR products were cleaned using 1X vol of AMPure XP Beads.
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bioRxiv - Cell Biology 2019Quote: ... FLAG-LC3B-K51R and FLAG-BIRC6-C4597A were generated using Q5® Site-Directed Mutagenesis Kit (New England Biolabs, E0552). The pSuper-Birc6-shRNA plasmid was generated by cloning shRNA for rat Birc6 (5’-CACCCCAGTAGTCATACCA-3’ ...
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bioRxiv - Cell Biology 2019Quote: ... A FLAG-tag (DYKDDDDK tag) followed by a stop codon was then inserted using the Q5 Site-Directed Mutagenesis Kit (New England BioLabs) according to manufacturer protocols ...
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bioRxiv - Genetics 2020Quote: ... The concentration and purity of genomic DNA was determined using a Nanodrop OneC (Thermo) and 500 ng of genomic DNA was bisulphite-converted using the EpiMark Bisulfite Conversion Kit (New England BioLabs) as per manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2019Quote: ... where C-terminal Myc-DDK tag was replaced by HIS-V5 tag using a NEBuilder HiFi DNA assembly kit (New England Biolabs). SHIP2 deletion in 293T cells was carried out by CRISPR/Cas9 technology (Ran et al ...
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bioRxiv - Microbiology 2019Quote: ... 5’ UTR reporter constructs were assembled using NEBuilder® HiFi DNA Assembly Cloning Kit (#E5520S New England Biolabs Ipswich, MA). All amplifications were carried out following the manufacturer’s guidelines ...
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bioRxiv - Microbiology 2019Quote: ... Fifty ng of cDNA from each sample were used an input for the NEBNext Ultra DNA Library Prep Kit for Illumina (New England Biolabs) according to the manufacturer’s recommendations ...
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bioRxiv - Genetics 2019Quote: ... The NEBuilder kit was used to fuse the F2 cDNA with linearized vector and transformed into 10beta competent cells (NEB). Site directed mutagenesis was used for missense mutation introduction39 and NEBuilder end homology joining was used to create internal deletions ...
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bioRxiv - Molecular Biology 2019Quote: ... Targeting constructs with appropriate homology arms were generated by Gibson assembly using the Gibson Assembly Master Mix kit (New England Biolabs), or in the case of single LoxP sites with 150 bp homology arms ...
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bioRxiv - Microbiology 2019Quote: ... Paired-end libraries were prepared according to the manufacturer’s recommendations using NEBNext Ultra II DNA Library Prep Kit (New England Biolabs, USA). The libraries were indexed with NEBNext Multiplex Oligos kits for Illumina (96 Index Primers ...
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bioRxiv - Molecular Biology 2019Quote: ... The cDNA was purified with AmpureXP beads and library was prepared with NEBNext Ultra II DNA Library Prep Kit Illumina (NEB) according to the protocol and sequenced on a HiSeq2500 (Illumina ...
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bioRxiv - Molecular Biology 2019Quote: ... ChIP-qPCR was performed to test the efficiency of the ChIP and libraries were prepared with NEBNext Ultra DNA Library Prep Kit Illumina (NEB) according to the protocol and sequenced on a HiSeq2500 (Illumina ...
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bioRxiv - Genomics 2019Quote: ... ChIP-seq libraries were prepared with the NEBNext Ultra II DNA Library Prep Kit for Illumina (E7645S, New England BioLabs) and Agencourt AMPure XP beads were used for size selection to generate the final libraries ...
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bioRxiv - Cancer Biology 2019Quote: ... 1 µg of genomic DNA from the tumor was used as input for library preparation using the NEBNext DNA Library Prep Kit (New England Biolabs). DNA was mechanically fragmented to 350 bp (Covaris ...
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bioRxiv - Neuroscience 2019Quote: ... Second strand synthesis was performed with the NEBNext mRNA Second Strand Synthesis Module kit – E6111S (New England Biolabs, Ipswich, MA). Full length cDNAs for each subunit were PCR amplified (MultiGene 60 OptiMaxTM Thermal Cycler - Labnet International Inc ...
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bioRxiv - Molecular Biology 2019Quote: ... This amplicon was used as a template for synthesis of capped and poly(A)-tailed RNA using the T7 HiScribe in vitro transcription kit (New England Biolabs) according to the manufacturer’s protocol ...
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bioRxiv - Developmental Biology 2019Quote: ... Subsequent pENTR clones were made by excising the original insert and replacing with new candidate enhancer fragments via Gibson Assembly (NEB HiFi Assembly Kit, New England Biolabs). The candidate enhancer fragments were shuttled to the transgenic vector pBPGUw [105] upstream of a minimal synthetic promoter sequence driving Gal4 using standard Gateway cloning techniques (LR Clonase ...
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bioRxiv - Molecular Biology 2019Quote: ... poliovirus-derived replicon plasmid prib(+)Luc-Wt was linearized with Mlu I (71) and in vitro transcribed with HiScribe T7 Quick High Yield RNA Synthesis Kit (NEB) as previously described (52) ...
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bioRxiv - Genetics 2020Quote: ... the sequence of the lead SNP construct was too GC rich to be synthesised and so the corresponding region was PCR amplified from a major allele homozygote and site-directed mutagenesis was used to create the alternate allele constructs (Q5 Site-Directed Mutagenesis Kit, NEB – used according to manufacturer’s instructions) ...
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bioRxiv - Microbiology 2019Quote: ... Three fragments of the viral genome were amplified by PCR using the Phusion® High-Fidelity DNA Polymerase kit (NEB) using primers mainly described previously [25] ...
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bioRxiv - Genomics 2019Quote: Each RNA-sequencing library was prepared from 200 ng of total RNA using the NEBNext Poly(A) mRNA Magnetic Isolation Module and the NEBNext Ultra RNA Library Prep Kit (New England Biolabs), following the manufacturer’s instructions and selecting for ~350 bp size fragments ...
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bioRxiv - Developmental Biology 2019Quote: ... the Cas9 site of the repair template was modified by introducing synonymous mutations either directly into the primers used for fragment amplification or separately by Q5 site directed mutagenesis kit (New England Biolabs). All DNA plasmids used for genome editing were transformed into DH5α competent cells and subsequently purified by miniprep (PureLink Quick Plasmid Miniprep Kit (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2019Quote: ... and family 2 baby 10 month) we performed enrichment with the NEB Next Microbiome Enrichment Kit (New England Biolabs, www.neb.com) and sequenced both the pre-enrichment and post-enrichment samples ...
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bioRxiv - Molecular Biology 2020Quote: ... Plasmid #1129 used to obtain transgenic fish line expressing LifeAct-frFAST fusion under the control of the cmlc2 enhancer28 was constructed using the NEBuilder assembly kit (New England BioLabs) in the pT22i vector for transgenesis previously used29.
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bioRxiv - Microbiology 2020Quote: Reverse transcription and amplification for figure 2 was performed using OneTaq One-Step RT-PCR Kit from NEB (cat. # E5315S). Both the OneTaq One-Step RT-PCR Kit and Luna Universal One-step RT-qPCR Kit (cat ...
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bioRxiv - Molecular Biology 2019Quote: ... and the pSpCas9(BB)-2A-mCherry (generated in house, by replacing the GFP with a mCherry reporter using the NEBuilding HiFi DNA Assembly Cloning Kit [New England Biolabs]). All sgRNAs sequences can be found in table S1.
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bioRxiv - Plant Biology 2019Quote: ... We constructed individually indexed genomic libraries using the NEBNext Ultra DNA Library Prep Kit (New England BioLabs, Ipswich, MA, USA), which were then target enriched8 and sequenced using a 1/3 lane of 150 bp paired ends on a HiSeq 3000 at the Oregon State University Center for Genome Research and Biocomputing Central Services Lab.
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bioRxiv - Evolutionary Biology 2019Quote: ... Libraries were prepared using the NEBnext Ultra Directional RNA Library Prep Kit for Illumina (New England Biolabs, Ipswich, MA, USA) and polyA enriched ...