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Citations for New England Biolabs :
6051 - 6100 of 9501 citations for FabFc ZAP rat Antibody Internalization Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2021Quote: ... phosphorylated and bead-purified in preparation for A-tailing using a custom NEB Paired-End Sample Prep Premix Kit (New England Biolabs). Illumina sequencing adapters were ligated for 15 minutes at 20°C and adapter-ligated products were then bead-purified and enriched with 10 cycles of PCR using primers containing a hexamer index that enables library pooling.
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bioRxiv - Cancer Biology 2021Quote: ... The PCR products were resolved by agarose gel electrophoresis and purified using the Monarch Gel Extraction kit (New England Biolabs). Purified PCR products were Sanger sequenced by Genewiz using the BPNT1_F_Seq primer ...
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bioRxiv - Molecular Biology 2019Quote: Total RNA was isolated as described above and sample preparation was performed using NEBNext Ultra Directional RNA Library Prep Kit for Illumina (E7420S/L; NEB) according to the protocol ...
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ISL2 is an epigenetically silenced tumor suppressor and regulator of metabolism in pancreatic cancerbioRxiv - Molecular Biology 2020Quote: ... RNA-Seq libraries were prepared using the NEBNext Ultra Directional RNA Library Prep Kit for Illumina (New England Biolabs # E7420S) by following the company's protocol ...
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bioRxiv - Cancer Biology 2021Quote: ... Libraries were constructed at the Center for Health Genomics and Informatics using the NEBNext Ultra II Directional RNA Library prep kit (New England Biolabs) with ribosomal RNA depletion ...
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bioRxiv - Cell Biology 2020Quote: ... Full-length 265 nt 5’ UTR of SARS-CoV-2 was subcloned to replace the 5’ UTR of human CMV in the pLV-mScarlet vector using a Hifi one-step kit (Gibson Assembly, NEB). Full-length ...
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bioRxiv - Genomics 2020Quote: ... The DNA was sheared to 650 bp using Covaris S220 and subsequently used for DNA-seq library preparation with NEBNext Ultra DNA Library Prep Kit (New England Biolabs). The paired-end sequencing datasets with 101 nt read length (PE101 ...
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bioRxiv - Cell Biology 2019Quote: ... One μg of purified RNA was reverse transcribed into cDNA using the Protoscript II Reverse Transcriptase kit (New England Biolabs). Quantitative real time polymerase chain reactions (qRT-PCR ...
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bioRxiv - Cell Biology 2020Quote: ... The PCR fragments were cloned into the linearized pUC19 vector using the Gibson Assembly Cloning Kit (E5510S; New England Biolabs).
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bioRxiv - Cell Biology 2020Quote: Site-directed mutagenesis of NES and hydrophobic-stretch coding sequences was performed using the Q5 site-directed mutagenesis kit (New England Biolabs) with either pCPLJJ50 ...
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bioRxiv - Cell Biology 2020Quote: ... the rRNA-depleted RNA was used to build the RNA-seq library with NEBNext Ultra II Directional RNA Library Prep Kit (New England Biolabs) according to the manufacturer’s manual ...
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bioRxiv - Cell Biology 2020Quote: ... 100 ng of total RNA from each sample was subjected to rRNA depletion with NEBNext rRNA Depletion Kit (New England Biolabs) according to the manufacturer’s manual ...
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bioRxiv - Cell Biology 2020Quote: ... The remaining steps of library preparation were done using the NEBNext Ultra II DNA Library Prep Kit for Illumina (New England BioLabs). Adapters and PCR primers were purchased from New England BioLabs ...
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bioRxiv - Cell Biology 2019Quote: ... and subsequently directional RNA-seq libraries were prepared using the NEBNext Ultra Directional RNA Library Prep Kit for Illumina (E7420, New England Biolabs). 75 bases paired end sequencing was performed on the Illumina HiSeq 2500 system ...
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bioRxiv - Cell Biology 2019Quote: Libraries were prepared from 10ng ChIP DNA using the NEBNext Ultra II DNA Library Prep kit for Illumina (E7645, New England Biolabs). 3 biological replicates were obtained for FLAG-Zbtb11 ChIP-seq in Zbtb11FLAG/FLAG E14 cells ...
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bioRxiv - Cell Biology 2020Quote: ... was then added between the MBP and FLOE1 coding sequences using the Q5 Site-Directed Mutagenesis Kit (New England Biolabs) with primers priTEV-FWD/REV (Table S3) ...
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bioRxiv - Cell Biology 2020Quote: ... The amplicons were cloned into PCR-linearized pPR3-N prey plasmid (Dualsystems Biotech) at the SfiI sites using the NEBuilder HiFi DNA Assembly Kit (New England Biolabs), and were transformed into DH5α competent cells and plated onto 48-well Bioassay Qtrays (Molecular Devices) ...
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bioRxiv - Cell Biology 2020Quote: ... Beads were resuspended in H2O used for library preparation with the NEBNext Ultra II Library Prep kit for Illumina (NEB). Beads were then washed 4 times using Tween wash buffer (5 mM Tris-HCl ...
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bioRxiv - Developmental Biology 2021Quote: ... 2011) was inserted into this p5E-βactin vector linearized with XhoI using the Gibson Assembly Cloning Kit (New England Biolabs). The optimised Gal4 KalT4 (Distel et al. ...
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bioRxiv - Developmental Biology 2021Quote: ... It was inserted into the pT1UciMP plasmid containing 14xUAS-ubc and Tol1 sites (Horstick et al., 2015) linearized with Xho1 using the Gibson Assembly Cloning Kit (New England Biolabs).
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bioRxiv - Genetics 2021Quote: ... ChIP-Seq libraries were prepared from 1-40 ng of DNA using the NEBNext Ultra II DNA Library Prep Kit for Illumina (NEB). Adaptors were diluted 10-fold prior to ligation ...
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bioRxiv - Developmental Biology 2021Quote: ... 250 ng equimolar PCR amplicons were combined for each biological sample and indexed using NEBNext Ultra II DNA Library Prep Kit for Illumina (NEB) with 6 PCR cycles ...
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bioRxiv - Genetics 2021Quote: ... cerevisiae strain ESM356-1 for in vivo recombinational cloning of the DNA constructs or the NEBuilder HiFi DNA Assembly Cloning kit (New England Biolabs) for their in vitro assembly ...
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bioRxiv - Genetics 2021Quote: DNA libraries were prepared from 5 ng of total genomic DNA using the NEBNext Ultra II FS DNA Library kit for Illumina (New England Biolabs). All volumes specified in the manufacturer’s protocol were divided by four ...
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bioRxiv - Genetics 2021Quote: ... high molecular weight genomic (HMW) gDNA was extracted from homozygous 1619P15 pupae using the Monarch HMW DNA extraction kit for tissue (NEB). The Cas9 Sequencing Kit (Oxford Nanopore Technology ...
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bioRxiv - Genomics 2020Quote: ... ligation and enrichment using the NEBNext® Ultra™II DNA Library Preparation kit (New England Biolabs Inc, Ipswich, MA). Furthermore ...
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bioRxiv - Genomics 2020Quote: ... Illumina adaptors for paired-end sequencing were ligated using the NEB Next DNA Library Prep Master Mix for Illumina kit (New England Biolabs). After each step ...
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bioRxiv - Microbiology 2021Quote: ... Total RNAs were used as templates for SYBR green-based one-step reverse-transcriptase quantitative PCR (RT-qPCR) using the NEB Luna One-Step RT-qPCR kit (New England Biolabs). All primers were validated by standard curve analysis and had PCR efficiencies ranging from 90-110% ...
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bioRxiv - Genomics 2021Quote: The subsequent fragmented gDNA was used as the starting input for the NEBNext Ultra II library prep kit for Illumina (E7645, New England Biolabs) following the manufacturer’s recommendations until the USER treatment step ...
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bioRxiv - Genomics 2021Quote: ... All the other gDNA from the bacteria presented in Table 1 were isolated using the Monarch genomic DNA purification kit (T3010S, New England Biolabs). Xp12 phage genomic DNA was obtained from Peter Weigele and Yian-Jiun Lee at New England Biolabs.
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bioRxiv - Genomics 2021Quote: The subsequent fragmented gDNA was used as the starting input for the NEBNext Ultra II library prep kit for Illumina (E7645, New England Biolabs) following the manufacturer’s recommendations until the USER treatment step ...
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bioRxiv - Genomics 2021Quote: PCR amplification of the samples was done following NEBNext Ultra II library prep kit for Illumina protocol (ER7645, New England Biolabs) and the NEBNext® Multiplex Oligos for Illumina®(E7337A ...
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bioRxiv - Microbiology 2021Quote: ... was added to in vitro transcription reactions according to the HiScribe T7 Quick High Yield RNA Synthesis Kit protocol (New England Biolabs). RNAs were included in phase separation assays at a final concentration of 16 nM (500:1 protein:RNA ratio).
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bioRxiv - Genetics 2021Quote: ... NEB #E7490) followed by library generation using NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (NEB E7760/E7765) per manufacturer protocols ...
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bioRxiv - Genetics 2020Quote: ... The eluted samples were treated with Proteinase K and DNA was purified using the Monarch PCR and DNA Cleanup Kit (NEB).
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bioRxiv - Microbiology 2020Quote: ... The NEBNext Directional RNA Library Prep Kit (Purified mRNA or rRNA Depleted RNA protocol; New England BioLabs, Beverly, MA, USA) and TruSeq Index PCR Primer barcodes (Illumina ...
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bioRxiv - Molecular Biology 2021Quote: ... Library preparation from mRNA and from miRISC-isolated RNA was performed using a NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (NEB) and Universal i5 and i7 primers following manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2021Quote: ... Library preparation from human islets total RNA (200ng) was performed using a NEBNext Low Input RNA Library Prep kit (NEB). Sequencing was performed on a HiSeq4000 using 75 bp paired end reads according to Illumina specifications ...
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bioRxiv - Microbiology 2020Quote: ... (88) by Gibson assembly (89) using the commercially available NEBuilder HiFi DNA Assembly Cloning Kit (New England Biolabs, Ipswich, MA). We introduced the lasR complementation construct into BB8 by electroporation (90 ...
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bioRxiv - Microbiology 2019Quote: ... PCR products were quantified and ∼80 ng of dsDNA was carried forward to library preparation using the NEBNext Ultra DNA Library Prep Kit for Illumina and NEBNext Multiplex Oligos for Illumina (New England Biolabs). Following twelve cycles of PCR enrichment ...
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bioRxiv - Microbiology 2019Quote: ... cDNA libraries with unique barcodes were generated from 100 ng enriched mRNA using the NEB Next UltraII Directional RNA Library Prep kit for Illumina (New England Biolabs). The individual cDNA libraries were assessed for quality and quantity by Qubit ...
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bioRxiv - Microbiology 2019Quote: In vitro transcribed 3X-FLAG tagged manY transcripts – wild-type or Δenh (1 pmol) were translated in vitro using the PURExpress translation kit (NEB). Translation reactions were stopped by adding Laemmli sample buffer (Bio-Rad) ...
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bioRxiv - Molecular Biology 2021Quote: ... Preparation of ChIP-seq library and ChIP sequencing was prepared with the NEBNext Ultra II DNA Library Prep Kit for Illumina (NEB), according to the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2021Quote: ... The 3xHA epitope tag in the knock-in construct was replaced by a 3xFlag epitope tag using the Q5 site-directed mutagenesis kit (NEB). The newly generated knock in sequence was amplified in a multi-step PCR reaction adding the terminator sequence from the pEv200 plasmid and BssHI and HindIII restriction site ...
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bioRxiv - Molecular Biology 2021Quote: ... DNA was sheared to an average fragment size of 350-400 pb using the Covaris S220 and sequencing libraries were prepared on beads using the NEB Next Ultra II DNA Library Prep Kit for Illumina and NEBNext Multiplex Oligos for Illumina (New England Biolabs) following instructions from the Arima Hi-C kit.
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bioRxiv - Molecular Biology 2021Quote: ... Full-length 265 nt 5′ UTR of SARS-CoV-2 was subcloned to replace the 5’ UTR of human CMV in the pLV-mScarlet vector using a Hifi one-step kit (Gibson Assembly, NEB). Full-length ...
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bioRxiv - Molecular Biology 2021Quote: RNA sequencing library was performed using the NEBNext® Ultra II Directional RNA Library Prep Kit for Illumina (NEB #E7760L), following manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2021Quote: ... several hundred cells exhibiting highest fluorescent intensity in the corresponding channel were collected and subjected to whole genome amplification using a commercially available whole genome amplification kit (WGA, New England BioLabs) followed by PCR amplification ...
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bioRxiv - Developmental Biology 2021Quote: ... PCR products were gel purified and transcribed in vitro using the HiScribe™ T7 High Yield RNA Synthesis Kit (NEB). Cas9 mRNA was synthesized from the pX330 plasmid using the mMESSAGE mMACHINE® T7 Ultra (Thermofisher ...
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bioRxiv - Developmental Biology 2021Quote: A plasmid encoding mouse ADAMTS6 with a C-terminal myc/his tag was generated previously (31) and used for site-directed mutagenesis (Q5 Site-Directed Mutagenesis Kit; E0554; New England BioLabs) to introduce Ala at Glu404 ...