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Citations for New England Biolabs :
6101 - 6150 of 9425 citations for Mouse Plectin PLEC ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2019Quote: ... A FLAG-tag (DYKDDDDK tag) followed by a stop codon was then inserted using the Q5 Site-Directed Mutagenesis Kit (New England BioLabs) according to manufacturer protocols ...
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bioRxiv - Genetics 2020Quote: ... The concentration and purity of genomic DNA was determined using a Nanodrop OneC (Thermo) and 500 ng of genomic DNA was bisulphite-converted using the EpiMark Bisulfite Conversion Kit (New England BioLabs) as per manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2019Quote: ... where C-terminal Myc-DDK tag was replaced by HIS-V5 tag using a NEBuilder HiFi DNA assembly kit (New England Biolabs). SHIP2 deletion in 293T cells was carried out by CRISPR/Cas9 technology (Ran et al ...
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bioRxiv - Microbiology 2019Quote: ... 5’ UTR reporter constructs were assembled using NEBuilder® HiFi DNA Assembly Cloning Kit (#E5520S New England Biolabs Ipswich, MA). All amplifications were carried out following the manufacturer’s guidelines ...
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bioRxiv - Microbiology 2019Quote: ... Fifty ng of cDNA from each sample were used an input for the NEBNext Ultra DNA Library Prep Kit for Illumina (New England Biolabs) according to the manufacturer’s recommendations ...
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bioRxiv - Genetics 2019Quote: ... The NEBuilder kit was used to fuse the F2 cDNA with linearized vector and transformed into 10beta competent cells (NEB). Site directed mutagenesis was used for missense mutation introduction39 and NEBuilder end homology joining was used to create internal deletions ...
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bioRxiv - Molecular Biology 2019Quote: ... Targeting constructs with appropriate homology arms were generated by Gibson assembly using the Gibson Assembly Master Mix kit (New England Biolabs), or in the case of single LoxP sites with 150 bp homology arms ...
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bioRxiv - Microbiology 2019Quote: ... Paired-end libraries were prepared according to the manufacturer’s recommendations using NEBNext Ultra II DNA Library Prep Kit (New England Biolabs, USA). The libraries were indexed with NEBNext Multiplex Oligos kits for Illumina (96 Index Primers ...
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bioRxiv - Molecular Biology 2019Quote: ... The cDNA was purified with AmpureXP beads and library was prepared with NEBNext Ultra II DNA Library Prep Kit Illumina (NEB) according to the protocol and sequenced on a HiSeq2500 (Illumina ...
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bioRxiv - Molecular Biology 2019Quote: ... ChIP-qPCR was performed to test the efficiency of the ChIP and libraries were prepared with NEBNext Ultra DNA Library Prep Kit Illumina (NEB) according to the protocol and sequenced on a HiSeq2500 (Illumina ...
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bioRxiv - Genomics 2019Quote: ... ChIP-seq libraries were prepared with the NEBNext Ultra II DNA Library Prep Kit for Illumina (E7645S, New England BioLabs) and Agencourt AMPure XP beads were used for size selection to generate the final libraries ...
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bioRxiv - Cancer Biology 2019Quote: ... 1 µg of genomic DNA from the tumor was used as input for library preparation using the NEBNext DNA Library Prep Kit (New England Biolabs). DNA was mechanically fragmented to 350 bp (Covaris ...
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bioRxiv - Neuroscience 2019Quote: ... Second strand synthesis was performed with the NEBNext mRNA Second Strand Synthesis Module kit – E6111S (New England Biolabs, Ipswich, MA). Full length cDNAs for each subunit were PCR amplified (MultiGene 60 OptiMaxTM Thermal Cycler - Labnet International Inc ...
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bioRxiv - Molecular Biology 2019Quote: ... This amplicon was used as a template for synthesis of capped and poly(A)-tailed RNA using the T7 HiScribe in vitro transcription kit (New England Biolabs) according to the manufacturer’s protocol ...
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bioRxiv - Developmental Biology 2019Quote: ... Subsequent pENTR clones were made by excising the original insert and replacing with new candidate enhancer fragments via Gibson Assembly (NEB HiFi Assembly Kit, New England Biolabs). The candidate enhancer fragments were shuttled to the transgenic vector pBPGUw [105] upstream of a minimal synthetic promoter sequence driving Gal4 using standard Gateway cloning techniques (LR Clonase ...
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bioRxiv - Molecular Biology 2019Quote: ... poliovirus-derived replicon plasmid prib(+)Luc-Wt was linearized with Mlu I (71) and in vitro transcribed with HiScribe T7 Quick High Yield RNA Synthesis Kit (NEB) as previously described (52) ...
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bioRxiv - Genetics 2020Quote: ... the sequence of the lead SNP construct was too GC rich to be synthesised and so the corresponding region was PCR amplified from a major allele homozygote and site-directed mutagenesis was used to create the alternate allele constructs (Q5 Site-Directed Mutagenesis Kit, NEB – used according to manufacturer’s instructions) ...
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bioRxiv - Microbiology 2019Quote: ... Three fragments of the viral genome were amplified by PCR using the Phusion® High-Fidelity DNA Polymerase kit (NEB) using primers mainly described previously [25] ...
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bioRxiv - Genomics 2019Quote: Each RNA-sequencing library was prepared from 200 ng of total RNA using the NEBNext Poly(A) mRNA Magnetic Isolation Module and the NEBNext Ultra RNA Library Prep Kit (New England Biolabs), following the manufacturer’s instructions and selecting for ~350 bp size fragments ...
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bioRxiv - Developmental Biology 2019Quote: ... the Cas9 site of the repair template was modified by introducing synonymous mutations either directly into the primers used for fragment amplification or separately by Q5 site directed mutagenesis kit (New England Biolabs). All DNA plasmids used for genome editing were transformed into DH5α competent cells and subsequently purified by miniprep (PureLink Quick Plasmid Miniprep Kit (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2019Quote: ... and family 2 baby 10 month) we performed enrichment with the NEB Next Microbiome Enrichment Kit (New England Biolabs, www.neb.com) and sequenced both the pre-enrichment and post-enrichment samples ...
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bioRxiv - Molecular Biology 2020Quote: ... Plasmid #1129 used to obtain transgenic fish line expressing LifeAct-frFAST fusion under the control of the cmlc2 enhancer28 was constructed using the NEBuilder assembly kit (New England BioLabs) in the pT22i vector for transgenesis previously used29.
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bioRxiv - Microbiology 2020Quote: Reverse transcription and amplification for figure 2 was performed using OneTaq One-Step RT-PCR Kit from NEB (cat. # E5315S). Both the OneTaq One-Step RT-PCR Kit and Luna Universal One-step RT-qPCR Kit (cat ...
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bioRxiv - Molecular Biology 2019Quote: ... and the pSpCas9(BB)-2A-mCherry (generated in house, by replacing the GFP with a mCherry reporter using the NEBuilding HiFi DNA Assembly Cloning Kit [New England Biolabs]). All sgRNAs sequences can be found in table S1.
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bioRxiv - Plant Biology 2019Quote: ... We constructed individually indexed genomic libraries using the NEBNext Ultra DNA Library Prep Kit (New England BioLabs, Ipswich, MA, USA), which were then target enriched8 and sequenced using a 1/3 lane of 150 bp paired ends on a HiSeq 3000 at the Oregon State University Center for Genome Research and Biocomputing Central Services Lab.
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bioRxiv - Evolutionary Biology 2019Quote: ... Libraries were prepared using the NEBnext Ultra Directional RNA Library Prep Kit for Illumina (New England Biolabs, Ipswich, MA, USA) and polyA enriched ...
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bioRxiv - Genetics 2019Quote: ... in accordance with the manufacturer’s recommendations and were validated using the Agilent High Sensitivity DNA assay on the Agilent Bioanalyzer 2100 system and quantified by NEBNext Library Quant Kit for Illumina (New England Biolabs). After clustering of the index-coded samples ...
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bioRxiv - Microbiology 2019Quote: ... The PCR products were cleaned up using the Monarch® PCR and DNA clean-up kit (New England Biolabs, USA). The PCR samples were mixed with 125 μl of the DNA Clean-Up Binding Buffer ...
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bioRxiv - Microbiology 2019Quote: ... samples were diluted to 5ng/uL and used for qRT-PCR with Luna One-Step Universal qPCR kit (NEB E3005). Gene specific primers for target transcripts were used at a final concentration of 400uM with 15ng of RNA ...
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bioRxiv - Genomics 2019Quote: ... Sequencing adapters and oligonucleotides used for PCR barcoding were from the NEBNext Multiplex Oligos for Illumina Kit (New England Biolabs, NEB). Prior to PCR amplification of the library ...
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bioRxiv - Developmental Biology 2020Quote: ... a total amount of 2µg of RNA per sample was used to prepare poly(A+)-enriched cDNA libraries using the NEBNext Ultra RNA library Prep Kit for Illumina (New England BioLabs) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2019Quote: ... Strand-specific RNA-Seq libraries were constructed using the NEBNext Ultra Directional RNA Library Prep Kit (NEB, catalogue number: E7420). The resulting library concentrations were quantified using the Nanodrop ...
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bioRxiv - Genetics 2019Quote: ... with a KAPA Hyper Prep Kit (KAPA, kk8502) and then processed by 3 μl of USER™ Enzyme (NEB, M5505L) at 37°C for 15 min to open up the loop ...
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bioRxiv - Genomics 2019Quote: ... followed by purification with Ampure beads and mRNA synthesis with the HiScribe™ T7 ARCA mRNA Kit (Cat. E2065S, NEB). Then the in vitro transcribed mRNAs were treated with DNase I (NEB ...
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bioRxiv - Genomics 2020Quote: ... was transcribed from a sgRNA-coding PCR product with a 5′ T7 promoter sequence using HiScibe T7 Quick High yield RNA Synthesis kit (NEB). The transcription was performed at 37 °C overnight and then purified by phenol ...
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bioRxiv - Neuroscience 2019Quote: ... RNA-seq libraries were prepared from the mRNA using the NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (New England Biolabs). Cluster generation was performed with the resulting libraries using the Illumina TruSeq PE Cluster Kit v4 reagents and sequenced on the Illumina HiSeq 2500 using TruSeq SBS Kit v4 reagents (Illumina) ...
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bioRxiv - Developmental Biology 2020Quote: ... mRNA-seq libraries were prepared with the NEBNext Ultra RNA library preparation kit with poly(A) mRNA magnetic isolation module (NEB) essentially according to manufacturer protocols ...
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bioRxiv - Plant Biology 2019Quote: ... DNA fragments were ligated to the Barcode Adapter from the barcode Kit 1D (EXP-PBC001, Nanopore) using Blunt/TA Ligase Master Mix (M0367L, NEB). Following purification with AMPure XP beads ...
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bioRxiv - Genetics 2020Quote: ... ChIP enrichment was determined by RT-qPCR (Supplementary Table 5) prior to addition of sequencing adaptors using NEBNext Ultra II DNA Library Prep kit for Illumina (New England Biolabs). ATAC-seq was performed as previously described76 ...
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bioRxiv - Genetics 2020Quote: ... all wells from a single plate were pooled and prepared for sequencing with the NEBNext Ultra II DNA Library Prep kit for Illumina (New England Biolabs). Plates were multiplexed and sequenced on the MiSeq platform (Illumina ...
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bioRxiv - Cancer Biology 2019Quote: ... The RNF114 C8A mutant was generated with Q5 site-directed mutagenesis kit according to manufacturer’s instructions (New England Biolabs, E0554S). Expression and purification conditions were optimized as reported previously79 ...
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bioRxiv - Genomics 2019Quote: ... or 100 ng of gDNA was assembled into paired-end libraries using the NEBNext Ultra II DNA Library Prep Kit (New England BioLabs). Approximately 60 libraries were pooled per lane prior to sequencing on a HiSeq 4000 machine to a target depth of 0.5×.
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bioRxiv - Systems Biology 2019Quote: ... RNA-Seq libraries were prepared from 1 μg of purified RNA using the NEBNext Ultra RNA Library Prep Kit for Illumina with NEBNext Poly(A) mRNA Magnetic Isolation Module (New England BioLabs). Sequencing was performed on an Illumina NextSeq 500 (75 cycles) ...
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bioRxiv - Molecular Biology 2019Quote: ... and set up in an overnight in vitro transcription reaction (HiScribe T7 Quick High Yield RNA Synthesis kit, New England BioLabs). The resulting RNA was purified using Silane beads (Life Technologies ...
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bioRxiv - Microbiology 2019Quote: ... Insert and dual indexed libraries were prepared using a modified NEBNext Ultra DNA II dual indexing kit (New England Biolabs) without size selection ...
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bioRxiv - Microbiology 2019Quote: ... Second-strand cDNA was synthesized in a reaction mixture containing Second-Strand Synthesis Kit (New England Biolabs; Ipswich, MA, USA). cDNA was purified using the Qiagen purification kit ...
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bioRxiv - Microbiology 2019Quote: Site-directed mutagenesis of low copy number vector p3187 harbouring siiA::HA under control of PsiiA was performed by using the Q5 SDM kit according to the manufacturer instructions (NEB) (Table S9) ...
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bioRxiv - Cell Biology 2019Quote: ... The CLN6ΔL2 construct was obtained by removing the codons for amino acids 155-222 using the Q5 Site-Directed Mutagenesis kit (New England Biolabs).
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bioRxiv - Genomics 2019Quote: ... Immunoprecipitated and input material was phenol-chloroform purified and ChIP-seq libraries were prepared using the NEBNext Ultra II DNA library kit (NEB) according to the manufacturer’s instructions with 35ng of DNA of each sample ...
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bioRxiv - Bioengineering 2019Quote: ... The pgRNAMPN142-400 plasmid is constructed by removing the gRNAMPN372 from the pgRNA-triple-target plasmid using the Q5 Site-Directed Mutagenesis Kit (NEB).