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Citations for New England Biolabs :
5901 - 5950 of 6874 citations for 7 6 7 dimethoxy 3 4 dihydroisoquinolin 1 yl 2 methyl 3 2 methylphenyl quinazolin 4 one since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genetics 2023Quote: ... Total RNA (1 µg) was reverse transcribed using ProtoScript II First Strand cDNA Synthesis Kit (NEB, E6560S) with primer UP0118 ...
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bioRxiv - Developmental Biology 2023Quote: ... and NEBNext® Multiplex Oligos for Illumina® (New England Biolabs Index Primers Set 1; NEB, E7335S). Samples were pooled and submitted to MSKCC Integrated Genomics Operation core for quality control and sequencing on Illumina HiSeq 4000 platform.
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bioRxiv - Developmental Biology 2023Quote: ... and NEBNext® Multiplex Oligos for Illumina® (New England Biolabs Index Primers Set 1; NEB, E7335S). Samples were pooled and submitted to MSKCC Integrated Genomics Operation core for quality control and sequencing on Illumina HiSeq 4000 platform.
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bioRxiv - Biophysics 2023Quote: ... The clarified and filtered lysate was loaded on 1 mL of IgG Sepharose fast flow resin (NEB). The column was washed with lysis buffer and TEV cleavage buffer (lysis buffer with 75 mM NaCl ...
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bioRxiv - Microbiology 2023Quote: ... pETduet-1::5′-flank_SpecPromoter_kanR_3′-flank and were subsequently digested from the plasmid using BamHI and NotI (NEB). The resultant insert (5′-flank_SpecPromoter_kanR_3′-flank ...
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bioRxiv - Cell Biology 2023Quote: ... 1-cell stage embryos were injected with two guides for each gene with Cas9 protein (NEB, M0646T). Tyrosinase was an injection control to validate the efficacy of the Cas9 protein ...
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bioRxiv - Biochemistry 2023Quote: ... 17 μl of the lysates was treated with 1 μl of Deglycosylation mix II (NEB, cat# P6044S) or distilled water ...
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bioRxiv - Genetics 2022Quote: ... using the NEBNext Multiplex Oligos for Illumina (Dual Index Primers Set 1) (New England Biolabs, Ipswich, MA). Paired-end 300 bp reads were generated on an Illumina MiSeq.
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bioRxiv - Genetics 2023Quote: ... and NEBNext® Multiplex Oligos for Illumina® (New England Biolabs Index Primers Set 1; NEB, E7335S). Samples were pooled and submitted to MSKCC Integrated Genomics Operation core for quality control and sequencing on Illumina HiSeq 4000 platform.
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bioRxiv - Genetics 2023Quote: ... and NEBNext® Multiplex Oligos for Illumina® (New England Biolabs Index Primers Set 1; NEB, E7335S). Samples were pooled and submitted to MSKCC Integrated Genomics Operation core for quality control and sequencing on Illumina HiSeq 4000 platform.
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bioRxiv - Microbiology 2023Quote: ... cDNA was synthesized from 1 µg of total RNA using the LunaScript RT SuperMix kit (NEB, UK) in accordance with the manufacturer’s protocol ...
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bioRxiv - Immunology 2022Quote: ... A first round of PCR was performed using 1× Standard-Taq magnesium free reaction buffer pack (NEB) with 2 mM MgCl2 (NEB) ...
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bioRxiv - Cancer Biology 2023Quote: ... then combined and treated with 1% SDS and 20 µg of proteinase K (New England Biolabs, #P8107S). The reaction was incubated for 1 h at 55°C ...
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bioRxiv - Genomics 2023Quote: ... 1 μl of Hia5 MTase (200 U)19 and 1.5 μl 32 mM S-adenosylmethionine (NEB B9003S) (0.8 mM final concentration ...
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bioRxiv - Cell Biology 2023Quote: ... Receptors were surface labeled by addition of SNAP-Surface Alexa Fluor 546 (1:1000, New England Biolabs) to DMEM F12 without serum and phenol red (21041-025 ...
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bioRxiv - Synthetic Biology 2023Quote: ... equimolar concentrations of the vectors were mixed with 1 µL I-SceI restriction enzyme (New England Biolabs) and 1 µL CutSmart buffer (10x ...
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bioRxiv - Microbiology 2023Quote: ... 20 uL of sheared DNA was then digested with 1 U of DpnI in cutsmart buffer (NEB) for 3 hours at 37°C ...
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bioRxiv - Neuroscience 2023Quote: ... 72°C – 1 min]) with NEBNext® High-Fidelity 2X PCR Master Mix (New England Biolabs, #M0541S) and barcoded Nextera primers (1.25 μM each ...
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bioRxiv - Microbiology 2023Quote: ... cDNA was reverse transcribed from 1 µg of total RNA using LunaScript RT Supermix kit (NEB #E3010) according to manufacturer’s instructions ...
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bioRxiv - Plant Biology 2024Quote: ... RNA (1 µg per sample) was transcribed to cDNA by the Protoscript®II reverse transcriptase (NEB). clpP ...
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bioRxiv - Molecular Biology 2023Quote: ... the GFP RNA was linked to the treated linker with T4 RNA ligase 1 (New England Biolabs). The obtained RNA was purified with the Monarch RNA purification kit (New England Biolabs) ...
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bioRxiv - Genetics 2024Quote: ... PCR samples were combined with 1 μL of 6X gel loading dye (New England Biolabs, Cat. # B7024S) per 5 μL of sample and loaded each the remaining wells ...
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bioRxiv - Biophysics 2024Quote: ... The resulting annealed structure was then combined with 1 µL of DraIII-HF (NEB; 100,000 units/mL) and 1 µL of BaeGI-HF (NEB ...
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bioRxiv - Cancer Biology 2024Quote: ... and libraries prepared using NEBNext Multiplex Oligos for Illumina Index Primer Set 1 (New England Biolabs, E7335S) and NEBNext Ultra II DNA Library Prep Kit for Illumina (New England Biolabs ...
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Cellular sialoglycans are differentially required for endosomal and cell-surface entry of SARS-CoV-2bioRxiv - Microbiology 2024Quote: ... followed by a secondary Alexa Fluor 555 conjugated anti-mouse IgG antibody (NEB #4413S; diluted 1:1000). For flow cytometry ...
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bioRxiv - Microbiology 2024Quote: ... The phages were then treated with 100 μg·ml-1 of Proteinase K (New England Biolabs, Whitby, Canada) with SDS to a final concentration of 2% (v·v-1 ...
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bioRxiv - Plant Biology 2019Quote: ... cDNA synthesis was performed for 1 h at 42°C using M-MuLV reverse transcriptase (200 U, NEB) followed by a heat-inactivation at 80°C for 5 min ...
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bioRxiv - Genetics 2021Quote: ... 1 μg of RNA was reverse transcribed in cDNA using random hexamers and M-MuLV reverse transcriptase (NEB). qPCRs were assembled with Absolute QPCR ROX Mix (Thermo Scientific ...
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bioRxiv - Molecular Biology 2021Quote: ... Backbone and annealed guide template were ligated for 1 h at room temperature using T4 DNA Ligase (NEB), 100 ng backbone ...
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bioRxiv - Evolutionary Biology 2021Quote: Hybridization with the reverse transcription primers was performed by adding 1 uL of SR RT Primer (NEB E7333A) to the above reaction and incubating at 75 C for 5 minutes ...
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bioRxiv - Evolutionary Biology 2021Quote: Both HLS-C and HLS-E protein at 1 mg/mL were incubated with human Furin (EC 3.4.21.75, P09958, obtained from NEB P8077) in digestion buffer (10 mM HEPES (pH 7.5) ...
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bioRxiv - Developmental Biology 2020Quote: ... 2014) by incubating 0.25 μl of plasmid with 1 μl of recombinant Cre recombinase (New England Biolabs M0298S) according to manufacturer’s instructions ...
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bioRxiv - Genomics 2020Quote: ... except that all ligations were supplemented with 1:40 RNAse inhibitor (ThermoFisher Ribolock or NEB Murine RNase Inhibitor) to prevent RNA degradation ...
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bioRxiv - Microbiology 2019Quote: ... and the mixture incubated at 37°C for 1 h prior to the transformation into XL10Gold cells (NEB) (Table S8) ...
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bioRxiv - Cell Biology 2019Quote: ... the cells were stained with DMEM containing 1 µM SNAP-Cell SiR (New England Biolabs, Ipswich, MA, USA) for about 10 minutes ...
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bioRxiv - Genetics 2021Quote: ... we used NEBNext® Multiplex Small RNA Library Prep Set for Illumina®(Set 1) (catalog#E7300S,NEB) (as described13) ...
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bioRxiv - Biochemistry 2020Quote: ... Cas9 RNPs were formed by incubating 1 pmol of sgRNA with 0.5 pmol of Cas9 protein in 1X NEBuffer™ 3.1 or 2.1 (NEB) at room temperature for 10 min ...
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bioRxiv - Microbiology 2021Quote: ... 8 μL of RNA was added per section to step 2.1 through to 2.11.11 of the NEBNext Single Cell/Low Input RNA Library Prep Kit for Illumina (NEB) using NEBNext Multiplex Oligos for Illumina (Index Primers Set 1 ...
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bioRxiv - Biophysics 2020Quote: ... followed by treatment for 1 h at 37 °C with 2.5 units of RNase-free DNase I (NEB). The sample was once again cleaned with RNeasy MinElute Cleanup Kit before applying on a 1% agarose gel for gel extraction and purification with QIAGEN gel extraction kit and elution with RNase free H2O ...
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bioRxiv - Synthetic Biology 2021Quote: ... we used either a DNA ladder (Low Molecular Weight DNA Ladder or 1 kb Plus DNA Ladder, NEB) or an RNA ladder (RiboRuler Low Range or High Range RNA Ladder ...
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bioRxiv - Synthetic Biology 2021Quote: ... The template was eliminated after the PCR reactions by addition of 1 µL DpnI (10,000 U/mL, NEB) to 25 µL PCR reactions and incubation at 37 °C for at least 1 h ...
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bioRxiv - Microbiology 2021Quote: ... the cap-1 structure was added to the 5’ end using the Vaccinia capping enzyme (New England Biolabs) and Vaccinia 2’-O-methyltransferase (New England Biolabs) ...
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bioRxiv - Physiology 2020Quote: ... and analysed by Western blotting using antibodies for TCF4/TCF7L2 (C48H11) (#2569, 1:500, Cell signalling, NEB, UK), phospho-AKT (#9271 ...
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bioRxiv - Plant Biology 2020Quote: ... PCR enrichment of adaptor ligated DNA was conducted using NEBNext Multiplex Oligos for Illumina (Set 1, NEB#E7335). The PCR reaction was purified using Agencourt AMPure XP Beads ...
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bioRxiv - Biophysics 2020Quote: Each reverse transcription (RT) reaction was performed using 50 ng total RNA in 1 mM dNTPs (NEB N0447S), 10% DMSO (Fisher ...
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bioRxiv - Microbiology 2021Quote: ... 100ng of vector was digested for 1 hour at 37°C with 10 U of BamHI-HF (NEB) to create a single double-stranded cut or 10 U Nb.BtsI (NEB ...
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bioRxiv - Cell Biology 2021Quote: SNAP-GCGR was detected with an anti-SNAP-tag rabbit polyclonal antibody (P9310S, New England Biolabs, 1/500) followed by goat anti-rabbit IgG H&L HRP (ab6271 ...
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bioRxiv - Biochemistry 2022Quote: 3’-PUA-DNA was generated by incubation of 1 μM 5’-FAM-labeled AP-DNA (FAM_U_35) with 20 U EndoIII/Nth (NEB) in Buffer B pH 7.0 at 37 °C for 60 m ...
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bioRxiv - Biochemistry 2022Quote: AP-DNA was prepared by incubating 1 μM uracil-containing oligonucleotide with 0.6 U UDG (New England Biolabs) (55 ...
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bioRxiv - Biochemistry 2022Quote: ... and each RV segment was then transcribed using 1 μg of linearized DNA with HiScribe T7 from NEB at 37°C for 3 hours ...