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Citations for New England Biolabs :
5701 - 5750 of 6874 citations for 7 6 7 dimethoxy 3 4 dihydroisoquinolin 1 yl 2 methyl 3 2 methylphenyl quinazolin 4 one since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genomics 2020Quote: ... All ligations were supplemented with 1:40 RNAse inhibitor (ThermoFisher Ribolock or NEB Murine RNase Inhibitor) to prevent RNA degradation.
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bioRxiv - Genomics 2020Quote: ... All ligations were supplemented with 1:40 RNAse inhibitor (ThermoFisher Ribolock or NEB Murine RNase Inhibitor) to prevent RNA degradation.
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bioRxiv - Molecular Biology 2019Quote: ... The Cap 1 25mer RNA was purified using Monarch RNA Cleanup kit (50 μg capacity, NEB). Complete methylation was verified by digestion of RNA with the Nucleoside Digestion Mix (NEB ...
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bioRxiv - Molecular Biology 2020Quote: ... and 1 μg of linearised DNA was then used in a HiScribe T7 synthesis kit (NEB), before DNase treatment and purification using a PureLink RNA mini kit (Thermo Fisher).
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bioRxiv - Bioengineering 2019Quote: ... The inserts were ligated to the phPB52 digested vector in 1× T4 DNA ligation buffer (NEB) with 30 ng of vector DNA with three molar excess of synthetic inserts ...
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bioRxiv - Molecular Biology 2019Quote: ... We used the NEBNext Multiplex Oligos for Illumina (Index Primers #1 - #24) (NEB, E7335S and E7500S) as the unique index primers ...
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bioRxiv - Microbiology 2019Quote: ... 1 μl Exonuclease I and 2.44 μl Antarctic Phosphatase buffer (New England BioLabs GmbH, Frankfurt, Germany) to 20 μl of the pooled PCR product ...
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bioRxiv - Biochemistry 2019Quote: Assays were conducted by incubating 0.1 and 1 μM SidJ in 1X Protein Kinase buffer (NEB), with 10 mM CaCl2 ...
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bioRxiv - Molecular Biology 2020Quote: ... After incubation for min at 55°C 1 μl T4 ligase (400,000 units/ml, NEB, M0202) was added ...
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bioRxiv - Genomics 2019Quote: ... Ligation was then performed in a ratio of 1:50 (vector:insert) with T4 DNA ligase (NEB) at room temperature for one hour and then transformed into NEB10 competent cells (NEB) ...
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bioRxiv - Genetics 2019Quote: ... 1 uL of cDNA or gDNA was amplified using Q5 High Fidelity 2X Master Mix (NEB). An indexing PCR was performed next using Nextera XT index kit primers (Illumina ...
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bioRxiv - Genomics 2021Quote: ... 500 ng of DNA was dissolved in 9 μL of 1 × CutSmart buffer (New England Biolabs) and sheared by pipetting 30 times with a pipette set at 8 μL with a P2/P10 tip ...
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bioRxiv - Microbiology 2021Quote: ... The pGHJ-TgApiAT6-1 and pGHJ-TgApiAT1 [27] plasmids were linearized by digestion with NotI (NEB) for 2 hr ...
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bioRxiv - Bioengineering 2020Quote: A 10x Cas12-gRNA complex mixture was prepared by pre-incubating 1 μM of LbCas12a (NEB) with 1.25 μM gRNA in 1x NEBuffer 2.1 at 37°C for 30 min ...
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bioRxiv - Biochemistry 2021Quote: ... coli cells (in 10.5 μl of nuclease-free water) were combined with 1 mM ATP (NEB), 40 U RNaseOUT ...
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bioRxiv - Biochemistry 2020Quote: ... the amplified DNA and the P4H-TM isoform 1 plasmid were both digested with BlpI (NEB) and XbaI (NEB ...
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bioRxiv - Neuroscience 2020Quote: ... and 7.5 μl of 1:1330 diluted micrococcal nuclease (11.3 gel units, New England Biolabs: NEB) for 5 min at 37 °C ...
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bioRxiv - Neuroscience 2020Quote: ... and 7.5 μl of 1:1330 diluted micrococcal nuclease (11.3 gel units, New England Biolabs: NEB) for 5 min at 37 °C ...
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bioRxiv - Microbiology 2021Quote: ... 1 µg of RNA was used for cDNA synthesis using the LunaScript RT SuperMix Kit (NEB) with and without reverse transcriptase (RT) ...
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bioRxiv - Cancer Biology 2020Quote: ... and ligated into pLKO.1 vectors using T4 DNA ligase and manufacturer protocols (New England Biolabs). DNA was transformed into Stbl3 bacteria for plasmid preparation (Thermo Fisher).
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bioRxiv - Cell Biology 2021Quote: ... Receptors were surface labelled by addition of Snap-Cell 647 SiR (1:1000, New England Biolabs) to the media for 20 min ...
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bioRxiv - Cell Biology 2020Quote: ... Equal amounts of crude mitochondria were mixed with 1/10 volume gel loading dye (7025, NEB) and subjected to Semi-Denaturing Detergent Agarose Gel Electrophoresis (SDD-AGE) ...
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bioRxiv - Microbiology 2020Quote: ... the supernatant was collected and mixed with 1 ml of amylose agarose resin (New England Biolabs) pre-equilibrated with buffer A per 50 ml of cell homogenate ...
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bioRxiv - Plant Biology 2021Quote: ... 1 μL xonuclease I and 2.44 μL Antarctic Phosphatase buffer (New England BioLabs GmbH, Frankfurt, Germany) to 20 μL of the pooled PCR product ...
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bioRxiv - Plant Biology 2021Quote: ... 1 mM ATP and 0.85 U of T4 DNA ligase (67 U/μL) (New England BioLabs). Ligation was performed at 37°C for 2 hours and 16°C for 16 hours ...
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bioRxiv - Genomics 2020Quote: ... each eluted insert was mixed with 50ng pDXinit-PAC in a molar ratio of 1:5 (vector:insert) in 10μl reactions and digested with 0.5μl SapI (NEB) for 1h at 37°C ...
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bioRxiv - Cell Biology 2020Quote: ... Samples were then treated 10 min with 1 U DNase I (New England BioLabs, Ipswich, MA) at 37°C ...
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bioRxiv - Microbiology 2021Quote: ... The ATP hydrolysis reaction was performed by incubating 0.3 µM HelR with 1 mM ATP (NEB), in a buffer containing 40 mM Tris ...
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bioRxiv - Biochemistry 2020Quote: ... In case of any potential overlap we treated our PCR reactions with 1 µL DpnI (NEB) to eliminate the donor plasmid ...
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bioRxiv - Biochemistry 2022Quote: ... Annealed circles were ligated with the addition of 1 μL of T4 DNA ligase (NEB M0202S) at room temperature for ∼4 hours ...
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bioRxiv - Cell Biology 2022Quote: ... for 30 min at room temperature and sequentially incubated with 1 µM Alexa647-SNAP-dye (NEB) and 1 mM DTT in PBS for 1 hour at room temperature ...
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bioRxiv - Cancer Biology 2022Quote: ... Reaction was supplemented with 1 mM dATP and 0.1 mM dCTP and 0.1 mM dGTP (NEB) and performed as follows in a thermocycler ...
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bioRxiv - Bioengineering 2022Quote: ... 1x RNA polymerase buffer and ∼1 U/µl of T7 RNA polymerase (New England Biolabs, Inc.). The samples were incubated at 37 °C for 6 hours ...
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bioRxiv - Cell Biology 2022Quote: ... cleared lysate was incubated without or with PNGase F (NEB, 1 μl per 40 μl lysate) for 30 min at 37 °C ...
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bioRxiv - Cell Biology 2022Quote: ... PV stock solution (1 mM) was prepared by adding 10 μl of 100 mM vanadate (NEB) and 50 μl of 100 mM hydrogen peroxide (diluted from a 30 % stock in 20 mM HEPES ...
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Adaptive translational pausing is a hallmark of the cellular response to severe environmental stressbioRxiv - Molecular Biology 2020Quote: ... Recovered footprints were dephosphorylated in a 15 µL reaction mixture (1 µL T4 PNK (NEB, M0201S), 10X T4 PNK buffer ...
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bioRxiv - Microbiology 2021Quote: ... immediately rescued in 1 ml of 37°C SOC outgrowth medium (New England Biolabs, cat#B9020S) and incubated with shaking at 37°C for 1 hour ...
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bioRxiv - Microbiology 2021Quote: ... 1 μL Exonuclease I and 2.44 μL Antarctic Phosphatase buffer (New England BioLabs GmbH, Frankfurt, Germany) to 20 μl of the pooled PCR product ...
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bioRxiv - Molecular Biology 2022Quote: ... 1 uL of cDNA was used in a 20 uL reaction with Phusion polymerase (NEB, M03530). The following cycling conditions were used for PCR prior to agarose gel electrophoresis ...
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bioRxiv - Systems Biology 2022Quote: ... for 48 hours and MtTether-SNAP was stained with 1:2000 SNAP-647 SiR (NEB, s9102) for 30 minutes ...
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bioRxiv - Microbiology 2019Quote: ... cells were suspended in 1 ml of super optimal broth with catabolite repression (S.O.C. medium) (NEB) and incubated at 37°C for 1 h ...
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bioRxiv - Molecular Biology 2020Quote: ... and indexed common primers (Vazyme, Cat.No.TD202) in 1 x Q5 High-Fidelity Master Mix (NEB, Cat.No.M0492). Then index PCR was performed as follow ...
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bioRxiv - Cell Biology 2019Quote: ... 40 nM down primer and 5 μM dNTP in 17 μl 1 x CutSmart buffer (NEB). The RNA and primers were incubated at a temperature gradient ...
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bioRxiv - Cell Biology 2020Quote: ... gel purified and ligated with pENTR (w158-1)-backbone by Quick Ligation kit (New England Biolabs) to create pENTR-GRET ...
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bioRxiv - Synthetic Biology 2020Quote: ... and immediately adding 1 U of β-agarase I per 100 ul of molten gel (NEB). The mixture was incubated at 42°C for 60 min to release DNA bound in the agarose matrix ...
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bioRxiv - Cancer Biology 2020Quote: shRNA was cloned into pLKO.1 vector using restriction enzyme sites AgeI (NEB, Cat. No. R3552) and EcoRI (NEB ...
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bioRxiv - Synthetic Biology 2021Quote: ... plasmid backbone DNA (1 μg) was digested using EcoRI-HF (20 units; R3101S, New England Biolabs) and SpeI-HF (20 units ...
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bioRxiv - Molecular Biology 2021Quote: ... This was followed by the addition of 1 µl 10x T4 RNA ligase reaction buffer (NEB), 1 µl NEB T4 RNA Ligase2 ...
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bioRxiv - Microbiology 2020Quote: ... Biotin-labeled RNP lysates were supplemented with streptavidin beads (1 mL per replicate, New England Biolabs) and captured by rotating at room temperature overnight ...
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bioRxiv - Cell Biology 2020Quote: β1-tubulin wild type construct was generated by the gibson assembly (HiFi Kit, New England Biolabs) of a TubB1 sequence fragment synthesised as a gBlock by Integrated DNA Technologies (IDT ...