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Citations for New England Biolabs :
5801 - 5850 of 9501 citations for FabFc ZAP rat Antibody Internalization Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genomics 2024Quote: ... Sequencing libraries were prepared with the NEBNext Ultra II DNA Library Prep Kit (New England Biolabs, Ipswich, Massachusetts, USA) according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2024Quote: ... primers oAA033-034 were used to remove mprF using the Q5 site-directed mutagenesis kit (NEB Cat. No. E0554S) following the manufacturer’s instructions ...
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bioRxiv - Microbiology 2024Quote: ... and prepared with the NEBNext Ultra II DNA Library Prep Kit for Illumina (New England Biolabs, Ipswich, MA, USA) according to the manual ...
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bioRxiv - Microbiology 2024Quote: ... Library preparation was performed using the NEBNext Ultra Directional RNA Library Prep Kit for Illumina (New England Biolabs; USA) with 1 μg total RNA per sample ...
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bioRxiv - Genomics 2024Quote: ... Library preparation was performed using the NEBNext Ultra II DNA Library Prep Kit for Illumina (New England BioLabs, E7645S) with the manufacturer’s instruction ...
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bioRxiv - Microbiology 2024Quote: ... double-stranded cDNA libraries were generated by using the NEBNext Ultra II RNA Library Prep Kit for Illumina (NEB) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2024Quote: ... Libraries were prepared by on-bead reactions using the NEB Next Ultra II DNA Library Preparation Kit (NEB, E7645S). The beads were separated on a magnetic stand ...
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bioRxiv - Bioengineering 2024Quote: ... and A-tailed using the NEB Next Ultra II FS DNA Library Prep Kit for Illumina (New England Biolabs). Linkers were then ligated to the DNA using the NEB Next Ultra II Ligation Master Mix and Ligation Enhancer (New England Biolabs) ...
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bioRxiv - Bioengineering 2024Quote: ... Nluc mRNA was synthesized using the HiScribe T7 high yield RNA synthesis kit (New England Biolabs Inc., MA, USA) with CleanCap Reagent AG (TriLink Biotechnologies ...
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bioRxiv - Cell Biology 2024Quote: ... the pellet was air dried and then resuspended in DNA Elution Buffer (Monarch DNA Gel Extraction Kit, NEB, USA). Since the RPS27AP5 sequence ...
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bioRxiv - Biochemistry 2024Quote: ... C-terminal Cx36 mutants were generated using the Q5 site directed mutagenesis kit (E0554S; New England Biolabs, Ipswitch, MA) and corresponding primer pairs introducing gaps into the Cx36 coding sequence ...
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bioRxiv - Biochemistry 2024Quote: ... All point mutations in PLD3 and PLD4 were generated using Q5-site directed mutagenesis kit (New England Biolabs #E0554S).
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bioRxiv - Cell Biology 2024Quote: ... Library sizes was measured using Bioanalyzer and pooled libraries concentration was calculated using NEBNext Lib Quant Kit (NEB Cat#). Libraries were pooled and sequenced on an Illumina NextSeq 550 using paired-end sequencing of 42 bases per read.
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bioRxiv - Cell Biology 2024Quote: MHC-ftz-Δi mRNA was synthesized in vitro using HiScribe T7 High Yield RNA Synthesis Kit (New England Biolabs) with MHC-ftz-Δi in pcDNA3.0 (30 ...
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bioRxiv - Neuroscience 2023Quote: ... The region of interest was amplified using Phusion® High-Fidelity PCR Kit (New England Biolabs, Cat. No. E0553S). The PCR product was purified with QIAquick PCR Purification Kit (Qiagen ...
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bioRxiv - Cell Biology 2023Quote: ... The RNA-Seq library was prepared by using the NEBNext Ultra II RNA Library Prep Kit for Illumina (NEB) and sequenced with the NextSeq 500/550 sequencing platform (performed at the NYUAD Sequencing Center) ...
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bioRxiv - Developmental Biology 2024Quote: ... NEBNext UltraTM II Directional RNA Library Prep Kit for Illumina and NEBNext UltraTM II DNA Library Prep Kit for Illumina according to the manufacturer’s instructions (New England Biolabs). Libraries were sequenced on a NextSeq2K (Illumina ...
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bioRxiv - Microbiology 2023Quote: ... xanthus cells grown on 1.5% agar supplemented with 1% CTT broth was extracted using the Monarch Total RNA Miniprep Kit (New England Biolabs). Briefly ...
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bioRxiv - Microbiology 2023Quote: ... Sequencing libraries were prepared using the NEB Next Ultra II DNA library prep kit for Illumina (New England Biolabs) following the manufacturer’s protocol ...
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bioRxiv - Microbiology 2023Quote: ... and cloned into pTM1 (a pDONR/Zeo based plasmid) downstream of the ACT1 promoter using Gibson cloning kit (NEB), and then introduced into pDG33 using Gateway cloning (Thermo Fisher Scientific) ...
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Deletion of MEC1 suppresses replicative senescence of the cdc13-2 mutant in Saccharomyces cerevisiaebioRxiv - Molecular Biology 2023Quote: ... 2017) using primers designed by NEBaseChanger and the Q5 Site-Directed Mutagenesis Kit (New England Biolabs, cat. no.: E0554S). The mutations were confirmed by DNA sequencing ...
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bioRxiv - Cancer Biology 2023Quote: ... Gαi2R179C and GαoAR243H mutants were constructed using pcDNA3.1 wild-type human ee-tagged-Gαi2- and ee-tagged-GαoA plasmids as template (cDNA Resource Center) and Q5 Site-directed mutagenesis kit (New England BioLabs). The lentiviral vector for tetracycline-inducible expression of the Gαi2R179C and GαoAR243H mutants was constructed by first cloning Gαi2R179C and GαoAR243H from pcDNA3.1 to pENTR vector (Thermofisher Scientific ...
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bioRxiv - Synthetic Biology 2023Quote: ... Reactions were purified according to the ssDNA cleanup protocol from the Monarch PCR cleanup kit (New England Biolabs; T1030L).
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bioRxiv - Neuroscience 2023Quote: ... Complementary DNA was synthesized from 20-60 ng of RNA using the ProtoScriptII First Strand cDNA Synthesis Kit (NEB).
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bioRxiv - Molecular Biology 2023Quote: Libraries were prepared for high-throughput sequencing according to manufacturer’s recommendations using the Ultra II DNA Library Prep kit (NEB). Samples were multiplexed and paired-end sequencing was performed using either a MiniSeq or NovaSeq 6000 (Illumina).
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bioRxiv - Neuroscience 2023Quote: ... Libraries were prepared from 10 ng of RNA using the NEBNext Ultra kit (New England BioLabs, Ipswich, MA, USA) and sequenced on an Illumina HiSeq 4000 with a 75 bp paired-end read ...
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bioRxiv - Evolutionary Biology 2022Quote: ... Sheared DNA was then adapter-ligated (AL) with NEBnext Ultra ii Library Preparation kit (New England Biolabs, Ipswich, MA). AL-DNA quality was again confirmed by Bioanalyzer high sensitivity DNA kit (Agilent ...
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bioRxiv - Cancer Biology 2023Quote: ... The cDNA libraries were prepared and indexed using the NEBNext Ultra RNA Library Prep Kit for Illumina (NEB, E7530L). Libraries were sequenced with the NovaSeq 6000 at a depth of 20 million paired-end 150 base pair reads per sample ...
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bioRxiv - Immunology 2022Quote: ... quantitative reverse transcription PCR (qRT-PCR) was conducted using Luna universal probe one-step RT-PCR kit (NEB, #E3006) on CFX96 Touch Real-Time Detection System on 96-well plates (Bio-Rad) ...
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bioRxiv - Microbiology 2022Quote: ... The pRSLepB–CutF(SM10) and pRSLepB–CutF(SM28) were constructed by using the Q5 mutagenesis Kit (NEB Lab, MA) with cutF(AP 10&28)-F/ cutF(AP-10aa)-R and cutF(AP 10&28)-F/cutF(AP-28aa)-R mutagenic primer pairs carrying the 10 and 28 aa C-terminal proline-rich segment of CutF respectively (Fig ...
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bioRxiv - Molecular Biology 2022Quote: ... Sequencing libraries were prepared using NEBNext® Ultra™ II Directional RNA Library Prep Kit for Illumina (NEB #E7760) coupled with AMPure XP bead purification (Beckman Coulter ...
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bioRxiv - Cell Biology 2023Quote: ... The library was generated using a NEBNext® Single Cell/Low Input RNA Library Prep Kit (New England Biolabs). RNAseq was performed using an Illumina NovaSeq 6000 ...
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bioRxiv - Biochemistry 2023Quote: ... Histone H2A chimeric mutant mimicking H2A.Z acidic patch (N94D/K95S) was generated using the Q5 mutagenesis kit (New England Biolabs). Monomethylated histone H4 lysine 20 (H4K20me1 ...
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bioRxiv - Microbiology 2023Quote: ... All mutations in pBAD T3 SAMase were introduced using Q5 Site-Directed Mutagenesis Kit or KLD Master Mix (NEB). Plasmids were transformed into chemically competent XL1-Blue cells (Evrogen) ...
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bioRxiv - Microbiology 2023Quote: ... P13-P19) (73) were carried out using Luna Universal One-Step RT-qPCR Kit (New England Biolabs; Frankfurt (Main), Germany ...
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bioRxiv - Molecular Biology 2023Quote: ... and RNA-seq libraries were prepared using a NEBNext Ultra Directional RNA Library Prep Kit for Illumina (NEB, E7420L). RNA-seq libraries were sequenced on an Illumina NextSeq500 Instrument with at least 10 million reads per library ...
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bioRxiv - Molecular Biology 2023Quote: ... Libraries were prepared with the NEBNext Ultra Directional RNA library Prep kit for Illumina (New England Biolabs, Ma, USA) and sequenced by high-throughput sequencing of pair-end 50 (PE50 ...
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bioRxiv - Microbiology 2023Quote: ... Samples were pooled into a library using the NEBNext Ultra II Directional RNA Library Prep Kit (New England Biolabs) and subsequently sequenced on Illumina NextSeq500 platform (75 bp ...
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bioRxiv - Microbiology 2023Quote: ... and PacBio sequencing were prepared using the NEB Monarch HMW DNA Extraction Kit for Cells and Blood (NEB #T3050) using the manufacturer’s protocol for fresh blood with slight modifications as we have previously described (28) ...
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bioRxiv - Genomics 2023Quote: ... DNA was extracted from 4.3×10^6 cells using the Monarch HMW DNA Extraction Kit for Cells and Blood (New England Biolabs) with 1400 rpm lysis speed ...
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bioRxiv - Cancer Biology 2023Quote: ... Resulting amplicons from the second PCR were column purified using Monarch PCR & DNA Cleanup Kit (New England Biolabs; NEB) to remove genomic DNA and first round PCR product ...
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bioRxiv - Cancer Biology 2023Quote: ... Resulting amplicons from the second PCR were column purified using Monarch PCR & DNA Cleanup Kit (New England Biolabs; NEB) to remove genomic DNA and first round PCR product ...
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bioRxiv - Biochemistry 2023Quote: ... Hairpin insertion (for IRES reporters) and all mutations were introduced using the Q5 Site-Directed Mutagenesis Kit (NEB # E0554S). We have previously published the use of the four HP-containing viral IRES nLuc reporters (32).
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bioRxiv - Developmental Biology 2023Quote: ... Genomic DNA from the spleen was extracted using the Monarch® HMW DNA Extraction Kit for Tissue (NEB #T3060) as per manufacturer’s protocol ...
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bioRxiv - Biochemistry 2023Quote: ... Single or multiple amino acid mutations in full-length GPR161 were generated using Q5 site-directed mutagenesis kit (NEB).
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bioRxiv - Developmental Biology 2023Quote: ... mRNA Magnetic Isolation Module and the NEBNext UltraTM II Directional RNA Library Prep Kit for Illumina according to the manufacturer’s instructions (New England Biolabs). Libraries were QC-checked on a Bioanalyzer 2100 (Agilent ...
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bioRxiv - Microbiology 2023Quote: ... The pools were cloned into SmaI-linearized AWP-029 with the NEBuilder® HiFi DNA Assembly kit (NEB, E2621) in two separate reactions as described above for the generation of single-gRNA mutants.
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bioRxiv - Microbiology 2023Quote: ... nLuc plasmids were linearized using SpeI and subjected to in-vitro transcription using HiScribe T7 kit (New England Biolabs), according to the manufacturer’s instructions ...
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bioRxiv - Evolutionary Biology 2023Quote: ... beads were directly used for off-bead PCR amplification using the Phusion High-Fidelity PCR kit (New England Biolabs) for 30 cycles with primers from library amplification (Rd1 ...
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bioRxiv - Microbiology 2023Quote: ... library preparation was done with the NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (New England Biolabs), library preparation included 11 cycles of PCR amplification ...