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Citations for New England Biolabs :
5801 - 5850 of 7781 citations for 7 Amino 1 3 dimethyl 1H 8H pyrido 2 3 d pyrimidine 2 4 5 trione since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2024Quote: ... 1 U/µl T4 PNK enzyme (NEB #M0201S) and 5 mM DTT (freshly added) ...
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bioRxiv - Plant Biology 2024Quote: ... and 1 µL of CutSmart Buffer (NEB #B6004S) and incubated for one hour at 37°C prior to a 20- minute heat inactivation at 80°C ...
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bioRxiv - Genomics 2024Quote: ... 1% v/v proteinase K (New England Biolabs), 50 mM Tris pH 8 (Ambion) ...
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bioRxiv - Biochemistry 2024Quote: ... and 1 U/µl Murine RNAse inhibitor (NEB)) ...
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bioRxiv - Cell Biology 2024Quote: ... 1 mL PNGase F (P0708L, New England Biolabs), and H2O as necessary to a final volume of 20 mL ...
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bioRxiv - Bioengineering 2024Quote: ... 1 μL dATP at 10 mM (NEB, N0440S), and 1 μL Taq polymerase (NEB ...
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bioRxiv - Biochemistry 2024Quote: ... anti-MBP (mouse, 1:30,000, New England Biolabs), anti-FLAG (mouse ...
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bioRxiv - Developmental Biology 2024Quote: ... and 1 μL USER Enzyme (NEB, Cat. M5505) to nick ...
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bioRxiv - Genetics 2024Quote: ... and 1 µL Taq DNA Polymerase (NEB, M0273) were added ...
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bioRxiv - Microbiology 2020Quote: ... 3 μL of in vitro transcribed 300 nM sgRNA or trL (produced as described in in vitro transcription protocol above) and 1 μL of 1 μM Cas9 nuclease (NEB, M0386S) were mixed and incubated for 10 minutes at 25°C to allow for RNP formation ...
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bioRxiv - Cell Biology 2021Quote: ... 1% NP-40, 1 mM DTT, 0.1 mg/ml cycloheximide, 1.25 mg/ml heparin, 400 U/ml NEB murine RNase inhibitor) and dounced ...
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bioRxiv - Genomics 2022Quote: ... Illumina libraries and DNA splint were mixed at a 1:1 ng ratio using NEBuilder HiFi DNA assembly Master mix (NEB #E2621). Any non-circularized DNA was digested overnight using ExoI ...
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bioRxiv - Microbiology 2020Quote: ... The flies were homogenised in 100 μl of TE-buffer pH 8 containing 1% Triton X-100 and 1% Proteinase K (NEB, P8107S). Homogenates were incubated for 3 h at 55°C followed by a 10 min incubation step at 95°C ...
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bioRxiv - Immunology 2022Quote: ... cleavage rates were transformed into standardized catalytic activities (1 “eqU” = equivalent to the catalytic activity of 1 U RNase HII (NEB ®) under standard conditions) ...
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bioRxiv - Molecular Biology 2024Quote: For proximity ligation, the pellet was resuspended into 500 μL of ligation mix (1× T4 DNA ligase buffer, 1× BSA (NEB, B9000S), 5000 CEU T4 DNA ligase (NEB ...
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A randomized multiplex CRISPRi-Seq approach for the identification of critical combinations of genesbioRxiv - Genetics 2023Quote: ... top and bottom oligonucleotides for a single R-S-R or dead end oligonucleotide pairs were combined 1 µL:1 µL and were phosphorylated in a 50 µL reaction using T4 Polynucleotide Kinase (NEB M0201L) for 2 h at 37 °C ...
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bioRxiv - Cell Biology 2023Quote: ... cells were live labeled with 1 μM ATTO 590-chloroalkane and 1 μM of SNAP-Cell 647-SiR (New England Biolabs; S9102S) (final concentrations) ...
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bioRxiv - Genomics 2023Quote: ... cells were finally resuspended in 400ul PSB-DTT at the concentration of ∼2e6 cells/100ul (PBS, 1% SUPERase In, 1% BSA (NEB B90000S), 1mM DTT) ...
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bioRxiv - Genomics 2024Quote: ... of 0.1 μM polyT-rootV2 oligo diluted in hybridization buffer (2x SSC, 1 mg/mL yeast RNA, 10% dextran sulfate, 1:1000 Murine RNase inhibitor NEB M0314) on a parafilm sheet placed on a glass plate ...
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bioRxiv - Molecular Biology 2024Quote: ... ssSynth primer was extended by adding 1 μL 10mM dNTP’s (Fisher cat#FERR0192) and 1 μL Klenow polymerase (NEB cat#M0210S) then incubating at 37°C for 1hr ...
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bioRxiv - Neuroscience 2024Quote: ... and end repair was performed by incubating chromatin-bound beads for 1 h at room temperature in 1× T4 ligase buffer with ATP (NEB, B0202), 0.25 mM dNTPs (Thermo Fisher Scientific ...
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bioRxiv - Synthetic Biology 2024Quote: ... The genes were assembled into the expression vector pMC-1-1-1 cut with NotI (Clark et al., 2022) through HiFi DNA assembly (NEBuilder HiFi DNA Assembly Master Mix NEB#E2621) into E ...
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bioRxiv - Bioengineering 2024Quote: ... To generate the proper overhangs for insertion into the Effector 1 position, clonal effectors were predigested with BsaI (600 ng clonal fragment, 1 μL BsaI-HFv2 (NEB, R3733L), 1 μL Quick CIP (NEB ...
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bioRxiv - Cancer Biology 2020Quote: ... and murine lung slices were incubated overnight with primary antibodies in 0.1% BSA as follows: anti-CDH1 (clone EP700Y, Biozol, 1:250) or anti-CDH1-Alexa-488 (clone 24E10, New England Biolabs, 1:50), anti-Twist1 (clone Twist2C1a ...
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bioRxiv - Microbiology 2021Quote: ... the purified cDNA was PCR amplified and barcoded using ONT’s PCR Barcoding Expansion 1-12 (EXP-PBC001) or PCR Barcoding Expansion 1-96 (EXP-PBC096) kits with LongAmp Taq 2x Mastermix (NEB, Ipswich, MA).
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bioRxiv - Microbiology 2020Quote: ... 50µl of lysed cells were transferred to a tube containing 450µL of digestion mix (1X NEB 1 buffer, 1% triton X-100). This process was repeated 11 times (total 12 tubes with 500 µl) ...
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bioRxiv - Biochemistry 2021Quote: ... the receptor was truncated at C-terminus at two positions (i.e. 1-342 and 1-351) by inserting a STOP codon by site-directed mutagenesis (NEB, Cat. no. E0554). The surface expression of WT and truncated receptor constructs were normalized to similar levels by DNA titration in HEK-293 cells ...
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bioRxiv - Molecular Biology 2021Quote: ... PCR products were visualized on a 1% agarose gel using the Quick-load Purple 1-Kb DNA ladder (New England Biolabs, Ipswich, MA). Primer3 Plus (Untergasser et al. ...
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bioRxiv - Cancer Biology 2020Quote: ... Cells were lysed in 1 mL ice-cold RNase-free cell lysis buffer supplemented with 1 μL RNase inhibitor (M0307S, NEB; Ipswich, MA). After storage in −80°C refrigerator for about 40 mins ...
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bioRxiv - Neuroscience 2023Quote: ... then incubated with mouse anti-CaMKIIα (6G9) (1:1000, Pierce Biotechnology, Catalog #MA1-048) and rabbit anti-Gaussia luciferase (1:1000, New England BioLabs, Catalog #E8023S) overnight at 4°C ...
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bioRxiv - Microbiology 2023Quote: ... μL supernatant were incubated with biotinylated DNA that was immobilized to streptavidin magnetic beads (1 pmol DNA to 1 μL beads; New England Biolabs, Ipswich, MA), as described in Meinhardt et al.79 ...
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bioRxiv - Molecular Biology 2021Quote: ... and 1 U of T4-ligase (New England Biolabs). After ligation ...
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bioRxiv - Evolutionary Biology 2020Quote: ... and 1 μl DeepVentR exo-polymerase (New England Biolabs). The PCR thermal regime consisted of 22 rounds of 94 °C for 20 sec ...
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bioRxiv - Genomics 2020Quote: ... and 1 μl (10 U/μl) of APE1 (NEB) in a final volume of 40 μl at 37°C for 20 min ...
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bioRxiv - Molecular Biology 2021Quote: ... and 1 μl RtcB RNA ligase (New England Biolabs) to a total volume of 20 μl ...
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bioRxiv - Molecular Biology 2020Quote: ... HRP conjugated (New England Biolabs, E8038S, dilution 1:10000); IRDye 680RD goat anti-mouse IgG (LI-COR ...
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bioRxiv - Molecular Biology 2020Quote: ... using 8 units of T4 RNA ligase 1 (NEB) and 8 nmol of ATP in a final volume of 8 μl for 1 h at 37°C ...
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bioRxiv - Molecular Biology 2020Quote: ... Cells were washed twice in 1× NEBuffer3.1 (NEB, B7003S) and resuspended in 342 μl of 1× NEBuffer3.1 ...
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bioRxiv - Genomics 2022Quote: ... and Quick-Load 1 kb Extend DNA Ladder (NEB) to aid size determination ...
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bioRxiv - Genomics 2020Quote: ... 1 μL of 5U/μL Klenow (New England Biolabs). The beads were incubated for 30 minutes at room temperature ...
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bioRxiv - Microbiology 2020Quote: ... 1 unit/μL of DpnI (NEB; catalogue number R0176S) was added in 5 μL of purified msDNA and digested overnight at 37°C ...
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bioRxiv - Genomics 2020Quote: ... 1 U/μl USER enzyme (both from NEB, USA), 6% v/v lymphoprep (STEMCELL Technologies ...
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bioRxiv - Synthetic Biology 2020Quote: ... and 1 µL of T4 DNA ligase buffer (NEB), and underwent 50 cycles of golden gate assembly (2 minutes at 42 °C ...
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bioRxiv - Synthetic Biology 2020Quote: ... 0.5 µL 1 mg/mL bovine serum albumin (NEB), 0.25 µL T4 DNA ligase at 400 U/µL (NEB) ...
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bioRxiv - Neuroscience 2020Quote: ... mouse anti-myc (NEB/Cell Signaling, 2276; 1:1500) o/n at 4 °C ...
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bioRxiv - Neuroscience 2020Quote: ... and 0.038 U μL−1 Lambda Exonuclease (NEB, M0262L)] to each well using the Mantis liquid handler and preamplified the cDNA using the following protocol ...
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bioRxiv - Bioengineering 2020Quote: ... 1 μL of Lambda Exonuclease (M0262S, New England Biolabs) and 2 μL of 10 X Lambda Exonuclease Buffer ...
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bioRxiv - Biochemistry 2021Quote: ... 1 μl/sample volume of T4 DNA ligase (NEB) was added to the master mix ...
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bioRxiv - Biophysics 2021Quote: ... re-suspended in 800 µL 1 × DpnII buffer (NEB) with 1.6% Triton X-100 ...
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bioRxiv - Neuroscience 2021Quote: ... NEBNext Multiple Oligos for Illumina Set 1 (NEB #E7335), 2 (NEB #E7500 ...