Labshake search
Citations for New England Biolabs :
5701 - 5750 of 10000+ citations for Mouse Dachshund Homolog 1 DACH1 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2022Quote: Complementary DNA (cDNA) encoding for Human CD36 (RC221976) was cloned using Hifi DNA assembly kit (NEB E5520S) into the pJFRC-MUH vector (Addgene ...
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bioRxiv - Evolutionary Biology 2023Quote: ... The sequencing libraries were prepared using the NEBNext Ultra RNA Library Prep Kit for Illumina (NEB, USA), and the transcriptome libraries were sequenced on an Illumina NovaSeq 6000 system (Novogene Co ...
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bioRxiv - Genomics 2023Quote: ... were added by using NEBNext Ultra II DNA library prep kit for Illumina (New England Biolabs, USA). Ligation products were purified by Ampure XP (Beckman Coulter ...
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bioRxiv - Molecular Biology 2022Quote: ... Libraries were cloned using the NEBNext Ultra Directional II RNA Library Prep Kit for Illumina (NEB, E7760), following the manufacturer’s instruction ...
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bioRxiv - Biochemistry 2023Quote: ... and Golden Gate cloning with the NEBridge® Golden Gate Assembly Kit (New England Biolabs, Ipswich, USA) according to the instructions provided by the manufacturer ...
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bioRxiv - Biochemistry 2023Quote: ... The mutants were generated using the Q5 site-directed mutagenesis kit (New England Biolabs, Ipswich, MA, USA), and plasmids were confirmed by Sanger sequencing (LGC Genomics) ...
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bioRxiv - Biochemistry 2023Quote: ... Sequencing libraries were prepared using the NEBNext Ultra II DNA Library Prep kit for Illumina (NEB E7645S). Library size distribution was monitored by capillary electrophoresis (Agilent 2100 Bioanalyzer ...
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bioRxiv - Biochemistry 2023Quote: ... extraction of genomic DNA with the Monarch® Genomic DNA Purification Kit (New England Biolabs, Ipswich, USA), Gibson assemblies with the NEBuilder® HiFi DNA Assembly Master Mix (New England Biolabs ...
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bioRxiv - Biochemistry 2023Quote: ... Sequencing libraries were generated using NEBNext® UltraTM RNA Library Prep Kit for Illumina® (NEB, USA) following the manufacturer‘s recommendations ...
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bioRxiv - Neuroscience 2023Quote: ... Sequencing libraries were made with the NEBNext Ultra II DNA Library Prep kit (New England Biolabs E7645L) and cleaned-up using SPRIselect beads (Beck-man Coulter B23317) ...
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bioRxiv - Neuroscience 2023Quote: 150 ng of total RNA was applied to the NEBNext rRNA Depletion Kit (New England Biolabs, #E6310L) according to the manufacturer’s protocol ...
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bioRxiv - Genomics 2023Quote: ... Single-end strand-specific libraries were prepared using NEBNext Ultra RNA library Prep Kit for Illumina (NEB) according to the manufacturer’s instructions ...
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bioRxiv - Animal Behavior and Cognition 2023Quote: ... and converted this mRNA to 280-300 bp cDNA fragments using the Ultra II Directional Kit (NEB). Unique sequencing adapters were added to each cDNA library for multiplexing (NEB).
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bioRxiv - Plant Biology 2023Quote: ... Sequencing libraries were generated using NEBNext® UltraTM RNA Library Prep Kit for Illumina® (NEB, USA) following manufacturer’s recommendations and index codes were added to attribute sequences to each sample ...
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bioRxiv - Genetics 2023Quote: ... Libraries were prepared using NEBNext Ultra II library preparation kits for Illumina (New England BioLabs, Ipswich, MA). Each pool was indexed with a unique NEBNext i7 adapter and an i5 adapter containing a degenerate barcode and PCR amplified with 12 cycles ...
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bioRxiv - Microbiology 2023Quote: ... Eluted RNA was depleted from ribosomal RNA using NEBNext rRNA Depletion Kit (Bacteria, New England Biolabs, MA) then used as template to generate cDNA using NEBNext Ultra RNA Library Prep Kit for Illumina (England Biolabs ...
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bioRxiv - Cell Biology 2023Quote: ... and RNA-seq libraries were generated using the Next Ultra RNA Library Prep Kit for Illumina (NEB) as described before (Berneking et al ...
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bioRxiv - Cancer Biology 2023Quote: RNA was isolated from cell lysates using the Monarch Total RNA Miniprep Kit (T2010S, New England BioLabs) according to the manufacturer’s recommendations ...
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bioRxiv - Microbiology 2023Quote: ... The sgRNAs were prepared using the EnGen sgRNA synthesis kit following the manufacturer’s protocol (New England BioLabs), and then associated with EnGen Spy Cas9 NLS protein (New England BioLabs ...
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bioRxiv - Microbiology 2023Quote: ... was constructed by assembling 3 DNA fragments using the NEBuilder HiFi DNA assembly kit (New England Biolabs). All 3 fragments were amplified from pTRL2-His-GrgA 36 using Q5 DNA polymerase (New England Biolabs) ...
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bioRxiv - Synthetic Biology 2023Quote: ... Libraries were prepared using the NEB Next® UltraTM DNA Library Prep Kit (New England Biolabs, U.S.A) with a target insert size of 350 nt and six PCR cycles ...
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bioRxiv - Molecular Biology 2023Quote: ... Tm or EPEC FliCD0 constructs were generated with the Q5 site-directed mutagenesis kit (New England Biolabs) following manufacturer recommendations ...
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bioRxiv - Molecular Biology 2023Quote: ... Sequencing libraries were generated using NEBNext® UltraTM RNA Library Prep Kit for Illumina® (NEB, USA) following manufacturer’s recommendations ...
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bioRxiv - Molecular Biology 2023Quote: In vitro translation was carried out with a custom PURExpress ΔRF-123 ΔRibosome kit (New England Biolabs), using ∼5 pmol of 5’-biotinylated and 3’-polyadenylated mRNA as a template ...
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bioRxiv - Microbiology 2023Quote: ... The metagenomic library was constructed using NEBNext® Ultra DNA Library Prep Kit for Illumina (NEB, USA). The samples were sequenced with the PE150 strategy on Illumina Hiseq Novaseq 6000 platform (commercial service ...
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bioRxiv - Molecular Biology 2023Quote: RT-qPCR experiments were performed with the Luna® Universal One-Step RT-qPCR Kit (NEB, #E3005E) with a final reaction volume of 10 μL per well ...
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bioRxiv - Genomics 2023Quote: ... An NGS library was generated using an NEB Ultra II DNA Library Prep kit (NEB, Ipswich, MA) with an Illumina compatible y-adaptor ...
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bioRxiv - Evolutionary Biology 2023Quote: ... Cloning step was performed using the NEBuilder HiFi DNA Assembly Cloning Kit (New England Biolabs, cat. #E5520S). The cloning was verified by restriction analysis and sequencing.
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bioRxiv - Developmental Biology 2023Quote: ... and resuspended in 20 µL H2O.Preparation of sequencing libraries was performed using NEBNext Ultra II kit (NEB) with 7 PCR cycles for library amplification ...
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bioRxiv - Evolutionary Biology 2023Quote: ... complemented by reagents from the NEBNext Ultra II library prep kit (New England Biolabs, Ipswich, Massachusetts, U.S.A.), and then sequenced at a MinION platform (Oxford Nanopore Technologies ...
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bioRxiv - Bioengineering 2023Quote: ... Then first-strand cDNA was produced using ProtoScript® II First Strand cDNA Synthesis Kit (NEB, E6560s) with random primers ...
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bioRxiv - Cancer Biology 2023Quote: The RNA library was constructed using NEB Next ® Ultra™ RNA Library Prep Kit (NEB, USA), following manufacturer’s recommended protocol ...
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bioRxiv - Microbiology 2023Quote: ... and RpoD control were assessed using the Luna Universal One-Step qRT-PCR kit (New England Biolabs) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: ... and the DNA library was prepared using the NEBNext® Microbiome DNA Enrichment Kit (New England Biolabs). The sequencing run was then performed on the Element AVITI sequencer (Element Biosciences ...
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bioRxiv - Neuroscience 2023Quote: ... . Libraries for Illumina short-read sequencing were prepared using the NEBNext Ultra II RNA library kit (NEB) according to manufacturer instructions ...
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bioRxiv - Biochemistry 2023Quote: ... The SKP1-FBXO22 mutant (C326A) was generated using a Q5 Site-Directed Mutagenesis Kit (NEB, cat. E0552). Both SKP1-FBXO22 WT and C326A were expressed in BL21(DE3 ...
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bioRxiv - Bioengineering 2023Quote: Complementary DNA (cDNA) was synthesized using the First Strand cDNA Synthesis Kit (New England BioLabs, Ipswich, MA) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2023Quote: ... the HiScribe® T7 High Yield RNA Synthesis Kit from New England Biolabs (NEB E2040S, Ipswich, MA) was used with the standard RNA synthesis protocol and incubated for 4 hours ...
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bioRxiv - Evolutionary Biology 2023Quote: ... and lysed using the Tissue Lysis buffer from the Monarch® Genomic DNA Purification Kit (NEB, #T3010S) with double the volume of lysis buffer and a 1 h incubation at 56 C ...
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bioRxiv - Genomics 2023Quote: Strand-specific RNA libraries were generated using the NEBNext Ultra II stranded library kit (New England Biolabs) combined with polyA-coupled beads (New England Biolabs ...
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bioRxiv - Cancer Biology 2023Quote: ... Total RNA was purified for all samples using a Monarch RNA Cleanup Kit (New England Biolabs, T2040L), according to manufacturer’s recommendations ...
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bioRxiv - Synthetic Biology 2023Quote: ... Quick Blunting Kit and the T4 DNA Ligase enzyme were purchased from New England BioLabs (NEB, Germany). The plasmid extraction kit was purchased from Qiagen GmbH (Hilden ...
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bioRxiv - Neuroscience 2023Quote: ... and Cttn-VC155 were generated by NEBuilder® HiFi DNA Assembly Cloning Kit (# E5520S, New England BioLabs), which enables the assembly of multiple inserts within a linearized vector in a one-step PCR reaction ...
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bioRxiv - Molecular Biology 2023Quote: ... RNA seq libraries were constructed from total RNA using the NEBNext Ultra II Directional RNAseq kit (NEB) with ribosomal RNA depletion ...
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bioRxiv - Molecular Biology 2023Quote: ... The resulting gRNAs were purified using the AMPure XP kit and treated with DNase I (NEB, UK). Concentration was evaluated on a Qubit system (Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2023Quote: ... and subsequently prepared for RNA-seq with the NEBNext Ultra Directional RNA Library Prep Kit (NEB #E7420) at GenomeScan ...
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bioRxiv - Microbiology 2023Quote: We performed RT-qPCR on select genes using the Luna Universal One-Step Kit (New England Biolabs) and QuantStudio 5 (Thermo) ...
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bioRxiv - Neuroscience 2023Quote: ... After quantification of individual libraries by q-PCR using the NEBNext library quant kit (New England BioLabs), all the samples were pooled in an equimolar manner ...
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bioRxiv - Genomics 2023Quote: Libraries were built on beads using the NEBNext Ultra II DNA Library Prep kit (NEB, Cat # E7645L). Biotin pull down was initiated py pipetting 20 μL Dynabeads My-One Streptavidin T1 beads (ThermoFisher Scientific ...
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bioRxiv - Genetics 2023Quote: ... RNA library preparation was done using a NEBNext Ultra II DNA Library Prep Kit for Illumina (NEB) on a Bravo WS automation system (Agilent) ...