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Citations for New England Biolabs :
5501 - 5550 of 9360 citations for Rat CD325 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genomics 2020Quote: ... The donor vector (mCTCF24-AID-donor-Neo) was constructed using PCR and Gibson Assembly Cloning kit (New England Biolabs). The insert cassette includes sequences that codes for a 5GA linker ...
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bioRxiv - Genomics 2019Quote: ... 12 cycles of PCR were performed as indicated by the NEBNext multiplex oligos for Illumina kit (New England Biolabs). PCR reactions were cleaned using Agencourt AMPure XP beads (Beckman).
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bioRxiv - Genomics 2019Quote: ... and then used for 350-bp paired-end library construction with the Next Ultra DNA library prep kit (NEB) for Illumina sequencing ...
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bioRxiv - Molecular Biology 2021Quote: ... ExRai AMPKAR T/A was generated via Gibson Assembly using NEBuilder HiFi DNA Assembly Kit (New England Biolabs E2621) using primers 1-2 ...
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bioRxiv - Molecular Biology 2021Quote: ... libraries were made using the NEBNext Ultra II Directional RNA Library Prep Kit following the polyA workflow (NEB, E7760). A custom protocol was used at Novogene for libraries preparation ...
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bioRxiv - Molecular Biology 2021Quote: ... the newly transcribed reporter mRNA was isolated using the Monarch RNA Cleanup Kit (New England Biolabs, Cat. No. T2040L), eluted with 1 mM sodium citrate pH 6.4 (Gene Link ...
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bioRxiv - Microbiology 2020Quote: ... RdRP_SARSr-F2 (GTGARATGGTCATGTGTGGCGG) and dRP_SARSr-R1(CARATGTTAAASACACTATTAGCATA) using the Luna Universal One-Step RT-qPCR Kit (New England Biolabs) and a CFX96 Real-Time System ...
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bioRxiv - Microbiology 2020Quote: ... cDNA libraries were prepared with the NEBNext® Ultra™ II Directional RNA Library Prep Kit (New England Biolabs) following manufacturer’s recommendations ...
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bioRxiv - Microbiology 2020Quote: ... Sequencing libraries were constructed using NEBNext Ultra RNA library Prep Kit for Illumina (New England Biolabs, Ipswich, MA, USA) and sequenced on the Illumina HiSeq 4000 platform with paired-end 150-bp reads production ...
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bioRxiv - Microbiology 2021Quote: ... Or reverse transcription and qRT-PCR were conducted in one step using 0.1 μg RNA as template with the Luna Universal Probe One-Step RT-qPCR kit (New England Biolabs) following manufacturer’s protocols ...
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bioRxiv - Microbiology 2020Quote: ... Sequencing libraries were generated using NEBNext UltraTM II DNA Library Prep Kit for Illumina (New England Biolabs, MA, USA), and the library quality was assessed on Qubit 3.0 Fluorometer (Life Technologies ...
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bioRxiv - Molecular Biology 2021Quote: ... DNA templates were removed by DNase I and the transcripts were purified by RNA purification kits (New England Biolabs). Purified RNA was quantified by measurement of OD260 using NANOPHOTOMETER (IMPLEN) ...
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bioRxiv - Molecular Biology 2020Quote: ... Samples were then prepared for sequencing using the NEBNext Ultra II DNA Library Prep Kit for Illumina (NEB, E7645) according to the manufacture’s protocol ...
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bioRxiv - Microbiology 2021Quote: ... Directional cDNA libraries were prepared using the NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (E7760, NEB). The libraries were sequenced using a HiSeq 1500 or 3000 system in single-read mode for 100 or 150 cycles ...
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bioRxiv - Microbiology 2021Quote: BsaI digested fragments were gel purified using Gel Extraction Kit (OMEGA) and ligated with T4 ligase (New England Biolabs) at room temperature for 1 h ...
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bioRxiv - Microbiology 2021Quote: ... Genomic DNA (Toxoplasma RH derivatives) was extracted for PCR using a Monarch Genomic DNA Purification Kit (New England Biolabs). Q5 Polymerase (New England Biolabs ...
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bioRxiv - Microbiology 2021Quote: ... RNA sequencing libraries were prepared using the NEBNext Ultra II RNA Library Prep Kit for Illumina following manufacturer’s instructions (NEB). Briefly ...
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bioRxiv - Cell Biology 2021Quote: ... Purified NLS-mNeptune2-T2A and ccz1 backbone PCR products were assembled together using NEBuilder HiFi Assembly cloning kit (NEB).
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bioRxiv - Developmental Biology 2020Quote: ... we used NEBnext Single Cell/Low RNA Library prep Kit for Illumina to generate sequencing libraries (New England BioLabs). Libraries were sequenced on Illumina NextSeq 500 system (Illumina).
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bioRxiv - Cell Biology 2021Quote: ... from E14 cells genomic DNA and cloned in pUC19 vector using Gibson Assembly Master Mix kit (New England Biolabs). The targeting construct was designed such that the guide RNA sequence used for the specific targeting was interrupted by the tag or contained silent mutations.
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bioRxiv - Genomics 2021Quote: ... RNA sequencing libraries were prepared via polyA selection using the NEBNext Ultra RNA Library Prep Kit for Illumina using manufacturer’s instructions (NEB). Briefly ...
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bioRxiv - Genomics 2021Quote: Libraries were prepared using an NEBNext® Ultra™ II FS DNA Library Prep Kit (New England Biolabs, Inc) with a fragmentation size incubation time of 15 min at 37°C ...
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bioRxiv - Genomics 2020Quote: ... The sequencing library was prepared with a NEBNext Ultra Directional RNA Library Prep Kit for Illumina (New England Biolabs). We purified polyA+ transcripts with the NEBNext Poly(A ...
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bioRxiv - Biochemistry 2020Quote: ... HQ116417) was cloned into a pOPINF vector (24) using the NEBuilder HiFi DNA Assembly Cloning Kit (New England BioLabs). The protein ...
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bioRxiv - Molecular Biology 2021Quote: ... NEBNext Ultra II DNA Library Prep Kit for Illumina was used to make the DNA libraries (NEB, #E7645S/L), which were sequenced on the Illumina NovaSeq (PE-150 ...
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bioRxiv - Genetics 2020Quote: ... Bisulfite conversion and library construction were carried out as before using the EpiMark Bisulfite Conversion Kit (NEB cat# E3318) according to manufacturer’s specifications ...
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bioRxiv - Molecular Biology 2021Quote: PCR products were subject to in vitro transcription using the HiScribe T7 High Yield RNA Synthesis Kit (E2040S, NEB), which relies on the T7 RNA polymerase to initiate transcription from a T7 promoter sequence (present in our fragments) ...
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bioRxiv - Bioengineering 2020Quote: ... followed by library preparation using the NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (New England Biolabs). Sequencing was performed using a NextSeq 500 (Illumina ...
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bioRxiv - Microbiology 2021Quote: ... pCMV-hnCoV-S-H501Y-Δ69/70 was obtained from pCMV-7.1-hnCoV-S-H501Y (forward: 5’-TCCGGCACAAACGGCACA-3’, reverse: 5’-GATGGCGTGGAACCATGTC-3’) via Q5 SiteDirected Mutagenesis Kit (NEB). All plasmids were confirmed by gene sequencing (BGI Beijing) ...
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bioRxiv - Microbiology 2022Quote: ... which was synthesized using the HiScribe T7 High Yield RNA Synthesis Kit according to manufacturer’s guidelines (New England Biolabs). Next ...
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bioRxiv - Microbiology 2022Quote: ... RNA passing quality control was converted to a sequencing ready library using the NEBNext Ultra II Directional RNA library kit with polyA selection as per the manufacturer’s instructions (New England Biolabs). The cDNA libraries were quantitated using qPCR in a Roche LightCycler 480 with the Kapa Biosystems kit for Illumina library quantitation (Kapa Biosystems ...
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bioRxiv - Immunology 2022Quote: ... 5µl of RNA (roughly corresponding to 1µg) was reverse transcribed to cDNA using the LunaScript RT SuperMix kit - dye based qPCR detection (NEB) following manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2022Quote: ... Libraries for RNA-seq were made with the NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (NEB) per the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2022Quote: ... then processed for RNA-seq library prep with the NEBNext Ultra II Directional RNA Library Prep Kit (NEB, E7530L), and finally sequenced on an Illumina NextSeq 500 ...
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bioRxiv - Cell Biology 2022Quote: ... The RNA-Seq library was prepared by using the NEBNext Ultra II RNA Library Prep Kit for Illumina (NEB) and sequenced with the NextSeq 500/550 sequencing platform (performed at the NYUAD Sequencing Center) ...
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bioRxiv - Neuroscience 2022Quote: ... and RNA was isolated according to the manufacturer’s instructions (Monarch® Total RNA Miniprep Kit; #T2010S New England Biolabs). As a quality control ...
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bioRxiv - Molecular Biology 2022Quote: ... RNA fragments were converted to library using NEBNext® Small RNA Library kit (ref#E7330S, New England Biolabs, UK) following the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2022Quote: ... prior to library preparation using the NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (New England Biolabs) with poly(A ...
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bioRxiv - Molecular Biology 2022Quote: ... ChIP samples were analyzed by qPCR or subjected to library preparation according to the manufacturer’s protocol (NEBNext Ultra II DNA Library Prep Kit for Illumina, NEB) using Dual Index Primers (NEBNext Multiplex Oligos for Illumina ...
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bioRxiv - Bioengineering 2022Quote: ... the 5S and Saba PCR products were purified using Monarch® PCR & DNA Cleanup Kit (5 μg) (NEB, # T1030). The purified DNA sequence was then analyzed through Sanger sequencing (GENEWIZ from Azenta ...
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bioRxiv - Bioengineering 2022Quote: ... In vitro transcription was performed using HiScribe™ T7 or SP6 High Yield RNA Synthesis Kit (New England Biolabs). RNA was subsequently purified using Monarch® RNA Cleanup Kit (New England Biolabs).
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bioRxiv - Cancer Biology 2022Quote: ... mRNA was isolated from 1.5 μg of total RNA using the kit NEBNext Poly(A) mRNA Magnetic Isolation Module (New England Biolabs). NGS libraries were prepared from the purified mRNA using the NEBNext Ultra II RNA Library Prep Kit for Illumina (New England Biolabs) ...
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bioRxiv - Biochemistry 2022Quote: ... The product bend was cut and DNA was extracted from the gel by Monarch DNA Gel extraction Kit (NEB). 32P-GTP labelled U1 RNA was prepared by in vitro transcription as described previously with addition of 0.5 μL of α-32P-GTP (3.3 μM ...
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bioRxiv - Biochemistry 2022Quote: ... The lysed cells were directly subjected to qRT-PCR using Luna Universal One-Step RT-qPCR Kit (NEB, #E3005L). The primer sets used in qRT-PCR to detect CI-MPR and CD-MPR mRNA are 5’-CATTCAGTGGGTGACTCTGTT-3’/5’-TGCTCTGGACTCTGTGATTTG-3’ and 5’-GCTCTAGTGAAGAGGCTGAAAC-3’/ 5’-GCACACCCTGAAGATGTAGATG-3’ ...
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bioRxiv - Biophysics 2022Quote: ... The 8C-8A(IL125) chimera cDNA construct was generated using the Phusion High-Fidelity PCR kit (New England BioLabs). All cDNAs were tagged on their carboxy terminus with Myc-DDK epitopes ...
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bioRxiv - Developmental Biology 2022Quote: ... Library preparation was performed with the NEB Next Ultra II DNA Library Prep Kit for Illumina (NEB, Cat #E7645S) using 25 μL of CUT&RUN DNA as input ...
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bioRxiv - Cell Biology 2022Quote: ... The amplicons (200 fmol) were first end-repaired with the NEBNext Ultra II End repair kit (New England Biolabs), then purified using AMPure XP beads (Beckman Coulter) ...
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bioRxiv - Cell Biology 2022Quote: ... ICL3 and S305A mutations were generated using back-to-back primers on pCDNA3.1-HCAR1-Flag vector by Q5 Site-Directed Mutagenesis Kit (NEB). Fluorogen activating peptide fusion to HCAR1 was synthesized with insertion of HCAR1 using BsmI site into pMFAP-β1 vector (Spectragenetics) ...
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bioRxiv - Cell Biology 2022Quote: ... Purified DNA was cloned into pAV0714 (Vještica et al., 2020) using NEBuilder® HiFi DNA assembly kit (NEB #E2621). After being verified by Sanger sequencing ...
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bioRxiv - Genomics 2022Quote: ... ChIP samples were processed for library preparation using the NEBNext Ultra II DNA Library Prep Kit for Illumina (NEB) following the manufacturer’s instruction and sequenced on a Next-Seq 500 Sequencer (Illumina ...