Labshake search
Citations for New England Biolabs :
501 - 550 of 653 citations for Brucella Blood Agar with Hemin and Vitamin K 15x100mm plate 26ml fill since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Molecular Biology 2023Quote: ... that can base pair to the 3’ nucleotide of the cDNA products as described.75,76 The reaction was stopped by adding proteinase K (1 unit; New England Biolabs) and 25 mM EDTA followed by clean-up with a Monarch PCR & DNA Cleanup Kit (New England Biolabs) ...
-
bioRxiv - Molecular Biology 2023Quote: ... and S2) or EDTA (25 mM final concentration) plus 1.8-units Protease K (all other Figures, New England Biolabs), and incubating at 25°C for 15 min ...
-
bioRxiv - Cell Biology 2024Quote: ... and TE buffer, DNA was reverse crosslinked by incubating beads in Elution buffer (1% SDS, 0.1M NaHCO3 and Proteinase K (NEB)) ...
-
bioRxiv - Biophysics 2023Quote: ... Reaction was terminated with 3 μL of stop buffer (5% sarkosyl, 0.025% bromophenol blue, 50% glycerol) and incubated with 1.6 units of Proteinase K (NEB, P8107S) for 15 minutes at 37 degrees before resolving on 1% agarose TAE gel stained with SybrSafe (Invitrogen ...
-
bioRxiv - Biochemistry 2022Quote: ... The samples were treated with 25 µg Proteinase K (1.25 µL of 20 mg/mL stock solution, New England Biolabs) and incubated at 50 °C for 20 minutes ...
-
bioRxiv - Cell Biology 2024Quote: ... Ligation reactions were quenched by addition of EDTA to 25 mM and proteins were digested by incubation at 37°C for 20 min with 0.1% SDS and 20 units/ml proteinase K (NEB). Unligated oligonucleotides were removed by gel filtration through a 50 cm x 0.7 cm Sepharose 4B column (Sigma ...
-
bioRxiv - Microbiology 2024Quote: ... treated or not for one hour at 56°C with 0.4 mg/mL proteinase K (P8107S, New England Biolabs) and then heated or not for one hour at 85°C.
-
bioRxiv - Biochemistry 2024Quote: ... The reaction mix was incubated at 37°C and 15 ul aliquots were removed at indicated time points, quenched into stop buffer (1.8% SDS, 10 mM EDTA) followed by Proteinase-K (2 units total) (NEB) treatment for 45 min at 50°C ...
-
bioRxiv - Genomics 2024Quote: ... samples were digested by incubation in reverse-crosslinking buffer (50 mM Tris pH 8.0, 50 mM NaCl and 0.2% SDS) with 1:50 proteinase K (NEB P8107S) for 30 min at 55 °C in the dark ...
-
bioRxiv - Biochemistry 2024Quote: ... The eluted protein:RNA complex was then de-crosslinked overnight at 65°C with constant shaking at 1000 rpm in the presence of 2.7 U/ml proteinase K (NEB) to digest the proteins ...
-
bioRxiv - Genetics 2020Quote: ... The eluted samples were treated with Proteinase K and DNA was purified using the Monarch PCR and DNA Cleanup Kit (NEB).
-
bioRxiv - Molecular Biology 2021Quote: ... after incubating the samples with 2 μl of RNase A (Thermo) at 37 °C for 2h and then with 8 μl of proteinase K (NEB) at 55 °C for 4h ...
-
bioRxiv - Molecular Biology 2021Quote: ... Genomic DNA was isolated with Proteinase K lysis buffer and 15 µg of DNA were digested overnight with 40 units of MspI (New England Biolabs). Digested DNA was separated on a 0.8% agarose gel ...
-
bioRxiv - Molecular Biology 2021Quote: ... 10 mM EDTA) at 65°C for 15 minutes and later the proteins were digested by 10U Proteinase K (NEB) at 37°C for 30 minutes ...
-
bioRxiv - Biochemistry 2021Quote: ... 150 μl of the remaining sample was mixed with 6 μl (1 μl per 25 μl sample volume) of Thermolabile Proteinase K (NEB), incubated at 37 °C for 30 minutes ...
-
bioRxiv - Biochemistry 2021Quote: ... The reaction was stopped with the addition of 0.2% SDS and 3 units of Proteinase K (New England Biolabs, #P8107S), and incubated for 1 hr at 37°C ...
-
bioRxiv - Microbiology 2022Quote: ... The ravA and viaA genes were PCR amplified from Escherichia coli K-12 MG1655 genomic DNA using Phusion polymerase (Biolabs). All PCR products were purified by DNA cleanup kit (Qiagen) ...
-
bioRxiv - Biochemistry 2022Quote: ... D-loops were deproteinized by adding 2 μL of 5% lithium dodecyl sulfate and 1 μL of 20 mg/mL proteinase K (New England Biolabs), and incubating the mixture at 37°C for 15 min ...
-
bioRxiv - Biochemistry 2022Quote: ... Reactions were performed at 80 °C for 60 minutes and terminated by the addition of 1 μL of Proteinase K (NEB). Cleavage fragments from pre-linearized substrates were purified using paramagnetic beads and quantified and analyzed as described above ...
-
bioRxiv - Cell Biology 2022Quote: Cellularized blastoderms were individually squashed and then lysed in 10 µL buffer containing Proteinase K and ThermoPol reaction buffer (New England BioLabs) for 45 min at 60°C then 10 min at 95°C ...
-
bioRxiv - Immunology 2022Quote: ... 2018” tagmentation mix were either sorted into plates containing RCB buffer for condition “hiSDSprotK-TWEEN” (2 x RCB: 100 mM Tris-HCl pH 8, 100 mM NaCl, 40 µg/mL Proteinase K (NEB), 0.4 % SDS (Sigma ...
-
bioRxiv - Developmental Biology 2020Quote: ... Cross-linking was reversed overnight by treating samples with 200 mM NaCl and 250 μg/mL Proteinase K (New England Biolabs) and incubating overnight at 65°C ...
-
bioRxiv - Microbiology 2021Quote: ... Bacterial lysis was completed by incubating for 3 h at 55°C in the presence of 1% SDS and 100 μg/ml proteinase K (NEB). Lysates from both methods were extracted twice with equal volumes of phenol– chloroform–isoamyl alcohol (25:24:1) ...
-
bioRxiv - Molecular Biology 2020Quote: ... 5 uL aliquots were removed at each time point and added to 2 uL 5 mg/mL Proteinase K (NEB) in 5% SDS ...
-
bioRxiv - Genomics 2021Quote: ... fixed and permeabilized cells (as described above) were suspended in a combination of Drop-seq lysis buffer spiked in with various concentrations of proteinase K (NEB), and PBS at equal volume ratio ...
-
bioRxiv - Microbiology 2023Quote: ... Samples were boiled for 10 min and 2 μL of 20 mg/ml proteinase K (New England Biolabs, Cat. #P8107S) were added ...
-
bioRxiv - Molecular Biology 2024Quote: ... adding nuclease-free water to a final volume of 1 L) with 1:1000 (vol/vol) proteinase K (New England Biolabs) at 37°C for 1 h ...
-
bioRxiv - Molecular Biology 2024Quote: ... 5 μL of the DNAse digestion product was then treated with 1 μL of Proteinase K (New England Biolabs, P8107S) in UltraPure water for a total reaction volume of 20 μL ...
-
bioRxiv - Neuroscience 2024Quote: ... the tissue was incubated in 5 mL of a clearing solution comprising Clearing Premix (Vizgen, PN 20300003) and 50 μL Proteinase K (New England BioLabs) at 37°C in a humidified benchtop incubator until the tissue was cleared.
-
bioRxiv - Neuroscience 2024Quote: ... and 0.5% (vol/vol) Triton X-100 in nuclease-free water and 1% (vol/vol) proteinase K (New England Biolabs, P8107S). The sample was digested in this buffer for ≥36h in a humidified ...
-
bioRxiv - Genomics 2024Quote: ... Genomic DNA was harvested by discarding culture media and adding lysis buffer consisting of 20 mM Tris pH 8 and 0.1% Triton X-100 (MilliporeSigma T9284) with 60 ng/mL of Proteinase K (New England Biolabs P8107S) added immediately prior to use ...
-
bioRxiv - Neuroscience 2023Quote: RNA was released from the beads through protein K lysis (Monarch® Total RNA Miniprep Kit; New England Biolabs, #T2010S). After lysis ...
-
bioRxiv - Cancer Biology 2024Quote: ... 1% sodium dodecyl sulfate) and reverse crosslinked with proteinase K overnight at 65C and subsequently treated with RNAse A (NEB) for 1 hour at 37°C ...
-
bioRxiv - Biochemistry 2023Quote: ... Reactions were quenched with the addition of an equal volume of 2X RNA loading dye buffer and 0.8 units of proteinase K from Tritirachium album (New England Biolabs Inc.) were added ...
-
Bio-orthogonal Glycan Imaging of Culture Cells and Whole Animal C. elegans with Expansion MicroscopybioRxiv - Bioengineering 2024Quote: ... Recombinant Muc1 or lubricin mixed with one of the following enzymes: 8 unit/mL of proteinase K (P8107S, New England Biolabs), 500 μg/mL of pronase (10165921001 ...
-
bioRxiv - Genomics 2022Quote: Samples were digested by incubation in reverse-crosslinking buffer (50 mM Tris pH 8, 50 mM NaCl, 0.2% SDS) with 1:50 proteinase K (NEB P8107S) for 8-16 hours at 55°C ...
-
bioRxiv - Immunology 2022Quote: ... DNA bound to STAT1 complexes was extracted using 1% SDS and 0.6 mg/ml of Proteinase K (New England Biolabs #P81075) at 60°C overnight ...
-
bioRxiv - Microbiology 2022Quote: ... washed with and resuspended into 1 ml PBS and incubated with 33 nM human neutrophil elastase (hNE, Elastin Products) or proteinase K at 20 μg/ml (NEB) at 37 °C ...
-
bioRxiv - Microbiology 2022Quote: ... Cells were then reverse crosslinked and lysed by adding 10μL of 10X Lysis-T (250mM EDTA, 2M NaCl, 10% Triton X-100) and 4μL of proteinase K (NEB, P8107S) and incubating at 55°C for 1 hour ...
-
bioRxiv - Synthetic Biology 2024Quote: ... Media was aspirated and cells were resuspended in 50 uL Cell Lysis Buffer (10X) and 0.5 uL of Proteinase K (New England Biolabs P8107S) per 96-well ...
-
bioRxiv - Cell Biology 2024Quote: ... Reactions were quenched by addition of EDTA to 50 mM and proteins were removed by treatment with proteinase K (20 units/ml) (NEB) and SDS (0.25% ...
-
bioRxiv - Cell Biology 2024Quote: ... Digest were quenched by addition of EDTA to 25 mM and proteins were digested by incubation at 37°C for 40 min following addition of SDS and Proteinase K (NEB) to 0.25% and 20 units/ml respectively ...
-
bioRxiv - Zoology 2024Quote: ... the worms were rehydrated with increasing concentration of PBS+0.1%Tween-20 and then subjected to the proteinase K (NEB, P8107S) treatment used at 10 μg/ml concentration for 2 min at room temperature ...
-
bioRxiv - Biophysics 2024Quote: ... Chromosome-bound proteins were degraded by flushing hypotonic solution supplemented with proteinase K (PKA, stock concentration of 800 units mL-1, NEB). The mix was prepared by adding 0.75 μL PKA to 20 μL hypotonic solution.
-
bioRxiv - Neuroscience 2024Quote: ... after removing the Gel Coverslip, slides were incubated in 5 ml warmed Clearing Solution (ref. 20300003, Vizgen) with Proteinase K (ref. P8107S, NEB) in a sealed petri dish for 24 hours at 37°C.
-
bioRxiv - Cell Biology 2024Quote: ... Reactions were quenched by addition of EDTA to 50 mM and proteins were removed by treatment with proteinase K (20 units/ml) (NEB) and SDS (0.25% ...
-
bioRxiv - Neuroscience 2024Quote: ... and nuclease-free water in a 5:10:1:34 ratio) supplemented with 0.8 U/µl Proteinase K (NEB P8107S) for 48-72 hours in a humidified 37 °C incubator.
-
bioRxiv - Cell Biology 2024Quote: ... Coverslips were gently removed from the gel with a spatula and the buffer was supplemented with proteinase K (New England Biolabs) at 8 U / mL at the last moment ...
-
bioRxiv - Molecular Biology 2023Quote: ... Genomic DNA was extracted with Monarch® HMW DNA Extraction Kit for Cells & Blood (NEB), digested with DpnI overnight at 37°C ...
-
bioRxiv - Developmental Biology 2024Quote: ... DNA was extracted using Monarch® HMW DNA Extraction Kit for Cells & Blood (NEB, T3050), according to the manufacturer’s instructions ...