Labshake search
Citations for New England Biolabs :
451 - 500 of 653 citations for Brucella Blood Agar with Hemin and Vitamin K 15x100mm plate 26ml fill since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Genomics 2024Quote: ... Spat tissue was then placed into a digestive solution containing lysis buffer and proteinase K (NEB). Samples were homogenized using a sterile pestle and digested at 56 °C for 2 hours ...
-
bioRxiv - Biochemistry 2020Quote: ... Diatom exconjugants were selected on 1/2L1 1% agar medium supplemented with 20 μg mL−1 phleomycin (Gold Biolabs) at a diel cycle of 14:10 at ~50 μE ms−1 light intensity.
-
bioRxiv - Microbiology 2022Quote: ... xanthus cells grown on 1.5% agar supplemented with 1% CTwas extracted using the Monarch Total RNA Miniprep Kit (New England Biolabs (NEB)) ...
-
bioRxiv - Microbiology 2023Quote: ... xanthus cells grown on 1.5% agar supplemented with 1% CTT broth was extracted using the Monarch Total RNA Miniprep Kit (New England Biolabs). Briefly ...
-
bioRxiv - Microbiology 2021Quote: ... 60 μL of each fraction was treated with 1 μL of thermolabile proteinase K (New England Biolabs) for 1 h at 37°C followed by inactivation for 10 min at 55°C.
-
bioRxiv - Molecular Biology 2021Quote: ... The pellet was resuspended using 100μL resuspension buffer (10mM Tris pH 7.0, 1% SDS and 0.01U/μL proteinase K (NEB)) for 30 minutes at 37°C ...
-
bioRxiv - Molecular Biology 2021Quote: ... Samples were then incubated at 45 °C for 2 hr with 5 μL of Proteinase K (NEB) in the presence of 40 mM EDTA (pH 8.0 ...
-
bioRxiv - Molecular Biology 2021Quote: ... the resin was resuspended in 100 μL of NLS Buffer and 10 μL of Proteinase K (NEB) and the sample was incubated at 50°C for 30 minutes while shaking at 1200 rpm ...
-
bioRxiv - Evolutionary Biology 2021Quote: ... Commercial competent cells were used for the K-12 DH10B strain (NEB® 10-beta high efficiency). Transformation of B REL606 and B REL607 was achieved via the chemical competence method ...
-
bioRxiv - Biochemistry 2020Quote: ... Reactions were quenched by adding SDS to 0.8% (v/v) and 80 mU Proteinase K (NEB P8107S). Cleavage products were diluted 4X in nuclease-free water and subjected to capillary electrophoresis (CE ...
-
bioRxiv - Molecular Biology 2020Quote: ... an additional 32 µL of MyK buffer and 8 µL of Proteinase K (NEB, #P8107S, Ispwich, MA) were added to each tube ...
-
bioRxiv - Neuroscience 2022Quote: ... Gelated samples were digested in 4 U/ml proteinase K buffer (New England Biolabs, Ipswitch, MA, USA) with 50 mM Tris pH 8.0 (Serva ...
-
bioRxiv - Biochemistry 2022Quote: ... mixing with 5 μL of stop buffer (50 mM EDTA and 2 mg/ml Proteinase K (NEB)) ...
-
bioRxiv - Cancer Biology 2021Quote: ... The beads were drained of all liquids before 2 mg/ml of Proteinase K (New England Biolabs) diluted in PK buffer (50 mM Tris-HCl pH 7.5 ...
-
bioRxiv - Genetics 2020Quote: ... IP and input samples were incubated with 10 μL of 800 U/mL Proteinase K (NEB P8107S) at 37° for 2 hours ...
-
bioRxiv - Genetics 2020Quote: ... 10 mL of a 10% BSA solution was incubated with 2.5 μL of Proteinase K (NEB P8107S) for 30 minutes at room temperature (∼22°C) ...
-
bioRxiv - Neuroscience 2023Quote: ... 1 mM EDTA pH 8.0 (Roth) and 1:100 Proteinase K (New England Biolabs 20 mg/ml)) in a 2 ml Eppendorf tube for at least 24 hours at RT in the dark ...
-
bioRxiv - Microbiology 2023Quote: ... The lysates (50 μl) were then incubated at 55°C with 1.25 μl proteinase K (NEB, P8107S). After 1h of incubation ...
-
TAD border deletion at the Kit locus causes tissue-specific ectopic activation of a neighboring genebioRxiv - Genetics 2022Quote: ... The ligation products were reverse crosslinked at 65°C overnight with proteinase K (New England Biolabs, P8107S) and DNA was extracted with phenol-chloroform ...
-
bioRxiv - Cancer Biology 2023Quote: ... then combined and treated with 1% SDS and 20 µg of proteinase K (New England Biolabs, #P8107S). The reaction was incubated for 1 h at 55°C ...
-
bioRxiv - Cancer Biology 2023Quote: ... and the samples were incubated overnight at 47℃ in clearing solution comprised of Protease K (NEB, P8107S) and Clearing Premix (Vizgen ...
-
bioRxiv - Microbiology 2024Quote: ... The phages were then treated with 100 μg·ml-1 of Proteinase K (New England Biolabs, Whitby, Canada) with SDS to a final concentration of 2% (v·v-1 ...
-
bioRxiv - Neuroscience 2024Quote: ... tube 2: 20 µL VLP suspension + 6 µL water + 1 µL Proteinase K (200 µg/mL, NEB), and tube 3 ...
-
bioRxiv - Neuroscience 2024Quote: ... the samples were cleared overnight at 37°C with a Clearing Solution containing Proteinase K (NEB P8107S) and Clearing Premix (Vizgen 20300003) ...
-
bioRxiv - Cell Biology 2024Quote: ... 0.8 M guanidine HCl) containing 8 units mL−1 of Proteinase K (P8107S, New England Biolabs Inc.). Gels were digested at room temperature overnight ...
-
bioRxiv - Microbiology 2022Quote: ... xanthus cells grown on 1.5% agar supplemented with 1% CTwas extracted using the Monarch Total RNA Miniprep Kit (New England Biolabs (NEB)) ...
-
bioRxiv - Genomics 2021Quote: ... phage particles were lysed at 56 °C for 2 h in 550 μL of lysis buffer (100 mM Tris-HCl at pH 8.0, 27.3 mM EDTA, 2% SDS, ~1.6 U Proteinase K [NEB #P8107]). After lysis ...
-
bioRxiv - Neuroscience 2021Quote: ... nuclei were resuspended in 200uL digestion buffer with 4 uL protease (an equal volume of protease K (NEB) was substituted if the manufacturer-provided protease was exhausted ...
-
bioRxiv - Molecular Biology 2020Quote: ... and crosslinks were reversed with 10% SDS and 0.6 mg/ml Proteinase K (New England Biolabs, Ipswich, MA) overnight with shaking at 65 °C ...
-
bioRxiv - Genetics 2024Quote: ... then resuspended and incubated in 20uL digestion buffer (1.1mL 1M Tris-HCl of pH6.8 in 50mL deionized water) with 2uL proteinase K (New England Biolabs) at 37°C for 15min ...
-
bioRxiv - Biochemistry 2024Quote: ... cells were harvested 3 days after electroporation and genomic DNA was extracted with 100 μL lysis buffer (10 mM Tris-HCl, pH 7.5, 0.05% SDS, 25 μg/mL proteinase K (NEB)) at 37 °C with incubation for 1 hour ...
-
bioRxiv - Cell Biology 2023Quote: ... treating each well with 100 μl of lysis buffer (0.45% NP40, 0.45% Tween20, 0.5x NEB Q5 PCR buffer, 0.1 mg/mL NEB Proteinase K) were incubated for 10 min at 55°C in and subsequently heated to 95°C for 10 min to inactivate the proteinase K ...
-
bioRxiv - Bioengineering 2022Quote: ... the IP buffer was adjusted to 1% SDS and 0.8 mg Proteinase K (New England Biolabs, Ipswich, MA) was added ...
-
bioRxiv - Genomics 2023Quote: ... This was performed with additional RNase (Astral Scientific, Taren Point, Australia) and proteinase K (NEB, Ipswich, MA, USA) treatment following manufacturer’s instructions ...
-
bioRxiv - Neuroscience 2024Quote: ... dried K-ε-GG peptides were resuspended in 20 µl of 1x CutSmart buffer (New England Biolabs, B7204S) and incubated with 2.5 u of QuickCIP phosphatase (New England Biolabs ...
-
bioRxiv - Molecular Biology 2024Quote: ... 5% glycerol) and one time with molecular grade water followed by incubation with proteinase K (New England Biolabs) at 55 °C for 30 minutes with constant shaking ...
-
bioRxiv - Molecular Biology 2024Quote: ... 5% glycerol) and one time with molecular grade water followed by incubation with proteinase K (New England Biolabs) at 55 °C for 30 minutes ...
-
bioRxiv - Plant Biology 2024Quote: ... This was incubated for 40 minutes at 65°C before 10µl of proteinase K (New England Biolabs; P8107S) was added and incubated for a further 20 minutes ...
-
bioRxiv - Cell Biology 2024Quote: ... samples were subjected to Proteinase K treatment and purified again using the Monarch®RNA Cleanup Columns (NEB) according to the manufacturer’s protocol ...
-
bioRxiv - Molecular Biology 2022Quote: ... The reactions were stopped by adding EDTA and the proteins were digested by adding Proteinase K (New England Biolabs) at 37℃ for 20min ...
-
bioRxiv - Molecular Biology 2020Quote: ... The un-crosslinked suspension was treated with 1 μg of RNase A at 37°C for 30 min and with 0.8 units of Proteinase K (NEB) at 45°C for 45 min ...
-
bioRxiv - Cell Biology 2022Quote: ... mixed with 10 μl of lysis buffer (10 mM Tris-HCl [pH 8.8], 1 mM EDTA, 25 mM NaCl, 1% SDS and 8U/ml Proteinase K, NEB) for 3 min at room temperature ...
-
bioRxiv - Biophysics 2020Quote: ... samples were incubated in digestion enzyme buffer consisting of digestion buffer supplemented with (0.5%) proteinase K (P8107S, New England Biolabs) and 5% Pronase (11459643001 ...
-
bioRxiv - Molecular Biology 2022Quote: ... The membrane was cut into pieces and PK buffer (100 mM Tris pH 7.4, 50 mM NaCl, 10 mM EDTA) was added together with Proteinase K (NEB). After 90 min of incubation at 37°C ...
-
bioRxiv - Evolutionary Biology 2021Quote: DNA was isolated from single fins by placing them in lysis buffer (10mM Tris pH 8, 100mM NaCl, 10 mM EDTA, 0.5% SDS) with Proteinase K (333µg/mL, NEB P8107S) at 55°C for between four hours and overnight ...
-
bioRxiv - Microbiology 2020Quote: ... Beads were resuspended in 48 μL elution buffer (50 mM Tris pH 8, 1 mM EDTA, 1% SDS) and 1.6 U Proteinase K (NEB), incubated at 55 °C for one hour and then 65 °C overnight ...
-
bioRxiv - Microbiology 2020Quote: ... The supernatant was diluted with one volume of water and treated with 0.5 μL of 20 mg/mL proteinase K (New England Biolabs) at 50°C for 2 hours ...
-
bioRxiv - Genomics 2021Quote: ... DNA was treated with RNase A for 1 hr at 37°C and Proteinase K (New England Biolabs, 8107) for 1 hr at 55°C ...
-
bioRxiv - Genetics 2020Quote: ... 200,000 of cells sorted in 400 ul of PBS were treated with 40 μl of proteinase K (NEB, P8107S) and 40 μl of 20% SDS and incubated at 65°C for 16 h in order to reverse the DNA crosslinking ...
-
bioRxiv - Biochemistry 2022Quote: ... The samples were treated with 25 µg Proteinase K (1.25 µL of 20 mg/mL stock solution, New England Biolabs) and incubated at 50 °C for 20 minutes ...