Labshake search
Citations for New England Biolabs :
5201 - 5250 of 9547 citations for QuantiChrom Phosphate Assay Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biophysics 2023Quote: ... Following mutagenesis for fluorescent labeling and/or creating RTT mutations using the Q5 mutagenesis kit (New England BioLabs), plasmids were transformed into E ...
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bioRxiv - Developmental Biology 2023Quote: ... Libraries were prepared using the NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (New England Biolabs) together with the NEBNext Poly(A ...
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bioRxiv - Cancer Biology 2023Quote: ... Mutagenesis of the HA-AHR plasmid was performed using Q5 Site-Directed Mutagenesis Kit (New England Biolabs, E0554S).
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bioRxiv - Genetics 2023Quote: N-terminal GST-fusion TF DBD proteins were expressed using the PURExpress in vitro transcription/translation kit (NEB) following the manufacturer’s recommendations ...
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bioRxiv - Molecular Biology 2023Quote: ... and 25 mM EDTA followed by clean-up with a Monarch PCR & DNA Cleanup Kit (New England Biolabs). Second-strand DNA synthesis was done by ligating 10 µM of a blunt-end duplex comprised of a 32-bp Illumina R1-3’SpC3 and 5’-phosphorylated R1R-3’SpC3 DNA (pre-annealed by incubating at 95°C for 3 min and slowly cooling to 25°C ...
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bioRxiv - Molecular Biology 2023Quote: Ribosomal RNA was depleted from total RNA preparations with the NEBNext rRNA Depletion Kit (Bacteria) (New England Biolabs). Total RNA libraries were prepared with NEBNext Ultra II Directional RNA Library Prep Kit for Illumina ...
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bioRxiv - Microbiology 2023Quote: ... Amplified gene inserts were assembled with amplified pEVS143 by Gibson assembly using NEB Gibson Assembly Cloning Kit (NEB) or NEBuilder HiFi DNA Assembly Master Mix (NEB) ...
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bioRxiv - Molecular Biology 2023Quote: The amplicons from the Q5 PCR step were cleaned up using Monarch® PCR & DNA Cleanup Kit (NEB). The purity and size of the DNA bands were visualized by running a 1% agarose gel ...
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bioRxiv - Molecular Biology 2023Quote: The dsRNAs were transcribed from these templates using HiScribe T7 high yield RNA synthesis kit (New England Biolabs). The dsRNAs were then treated with DNase and purified using RNA Clean & Concentrator-25 (Zymo Research).
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bioRxiv - Genomics 2023Quote: Genomic DNA was extracted from mESCs and LCLs using the Monarch Genomic DNA Purification Kit (New England Biolabs) according to the manufacturer’s instructions ...
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bioRxiv - Genomics 2023Quote: ... the products of PCR1 were purified using the Monarch PCR & DNA Cleanup Kit (New England Biolabs, no. T1030L) and deep sequenced at the MGH DNA Core ...
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bioRxiv - Genomics 2023Quote: ... WGBS dual indexed libraries were generated using NEBNext Ultra DNA library Prep kit for Illumina (New England BioLabs) according to the manufacturer’s instructions with modifications ...
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bioRxiv - Immunology 2023Quote: The NEBNext Ultra II directional RNA library prep kit for Illumina (New Englands Biolabs Inc., Ipswich, MA, USA) was used to prepare mRNA sequencing libraries ...
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bioRxiv - Microbiology 2023Quote: ... Libraries were constructed using the NEBNext Ultra II FS DNA Library Prep Kit for Illumina (New England BioLabs) in combination with NEBNext Multiplex Oligos for Illumina (96 Unique Dual Index Primer Pairs ...
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bioRxiv - Genomics 2023Quote: ... Linearized CROP-seq-optiI and amplified oligonucleotides were assembled using the NEBuilder HiFi DNA assembly cloning kit (NEB) with the inserts at 2 fold molar excess followed by multiple transformations into NEB stable competent E.Coli (NEB ...
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bioRxiv - Bioengineering 2023Quote: ... Stranded mRNA libraries were prepared using the NEBNext II Ultra Directional RNA Library Prep Kit (NEB, Cat# E7760L) and NEBNext Poly(A ...
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bioRxiv - Bioengineering 2023Quote: ... and RNA-seq libraries were constructed using NEBNext Ultra II RNA Library Prep Kit for Illumina (NEB #E7770) following manufacturer’s instructions ...
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bioRxiv - Bioengineering 2023Quote: DNA oligos of sgRNAs and x-gRNAs were designed according to the EnGen sgRNA Synthesis Kit (NEB #E3322) to add 5’-T7 RNA polymerase promoter sequence and 3’-Cas9 crRNA sequence and were purchased from Integrated DNA Technologies IDT then resuspended to a stock concentration of 100 μM ...
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bioRxiv - Biochemistry 2022Quote: ... -R98E/E127A) were generated by introducing point mutations using the Q5 site-directed mutagenesis kit (New England BioLabs), following manufacturer’s instructions ...
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bioRxiv - Biochemistry 2023Quote: ... All DNA fragments were fused in pCR2.1-TOPO (genome integration cassette) or pGRB2.0 (rescue plasmids) vectors using a Gibson assembly kit (New England Biolabs) and validated by sequencing ...
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bioRxiv - Microbiology 2023Quote: ... Subsequently the NEBNext® Ultra™ II Directional RNA Library Prep Kit for Illumina® (New England Biolabs) was used to generate the RNA libraries ...
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bioRxiv - Cell Biology 2023Quote: ... ChIP libraries were prepared using NEBNext Ultra II DNA Library Prep Kit for Illumina per manufacturer’s instructions (NEB). Sequencing was carried out on Illumina’s NextSeq 500 platform to produce 75 bp single-end reads at a minimum of 50 million reads per sample ...
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bioRxiv - Cancer Biology 2022Quote: ... Sequencing libraries were generated using NEBNext® Single Cell/Low Input RNA Library Prep Kit for Illumina (NEB) following the manufacturer’s recommendations ...
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bioRxiv - Cancer Biology 2023Quote: ... RNA-seq library was constructed with NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (NEB; E7765) through the polyA mRNA workflow ...
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bioRxiv - Cell Biology 2023Quote: ... cDNA or genomic sequence of each gene was amplified by Phusion High-Fidelity PCR kit (New England BioLabs), cloned to pDonor 221 ...
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bioRxiv - Cell Biology 2023Quote: ... HEK293 libraries were generated using NEBNext Ultra RNA Library Prep Kit for Illumina (New England Biolabs, Cat. #E7770) or NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (#E7760) ...
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bioRxiv - Genomics 2022Quote: ... Three library construction were performed with the NEBNext DNA Library Prep Kit (New England Biolabs, Ipswich, Massachusetts, USA). The sequencing was carried out by Illumina HiSeq 2000 platform (Illumina ...
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bioRxiv - Genomics 2022Quote: ... Metagenomic libraries of three species were prepared with NEBNext Ultra II DNA Library Prep Kit (BioLabs, New England) with a target insert length of 350 bp and sequenced on Illumina NovaSeq 6000 S4 (2×150bp reads) ...
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bioRxiv - Genomics 2022Quote: ... The BxbI attP site was then added using the Q5 Site-Directed Mutagenesis Kit (Q5 SDM, NEB #E0554). To add a library of diverse random barcodes ...
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bioRxiv - Genomics 2022Quote: ... DNA libraries were prepared from validated ChIP material using NEBNext Ultra II DNA library kit (New England Biolabs) protocol without size selection ...
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bioRxiv - Genomics 2023Quote: Genomic DNA from a RCC bull was extracted using the Monarch Genomic DNA Purification Kit (New England Biolabs) according to the manufacturer’s guidelines ...
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bioRxiv - Cell Biology 2023Quote: ... RNAseq libraries were prepared for Illumina using the NEBNext Ultra-Directional RNA library preparation kit (New England Biolabs). Library quality was confirmed using an Agilent BioAnalyzer 2100 and quantified using the NEBNext Library Quant Kit for Illumina (New England Biolabs) ...
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bioRxiv - Evolutionary Biology 2023Quote: ... DNA libraries were constructed using the NEBNext Ultra II DNA Library Prep Kit by New England Biolabs (NEB) and the NEBNext Multiplex Oligos for Illumina (Index Primers Set 1 ...
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bioRxiv - Genetics 2022Quote: ... The sequencing library was prepared according to the NEBNext Ultra II DNA Library Prep Kit for Illumina (NEB). Specifically ...
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bioRxiv - Genetics 2023Quote: ... RNA was converted to cDNA using a ProtoScript® II First Strand cDNA Synthesis Kit (NEB Cat # E6560L). RT-qPCR was performed using a QuantStudio 3 96-well 0.2 ml block instrument and PowerUp SYBR Green Master Mix (ThermoFisher Cat # A25741) ...
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bioRxiv - Immunology 2023Quote: ... The pEGIP-derived packaging vector pEGIP-hACE2 was generated by homologous recombination using the NEBuilder kit (NEB, E5520). The vector backbone was amplified using oligonucleotides with appropriate hACE2 sequence overhangs ...
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bioRxiv - Molecular Biology 2023Quote: ... CUT&RUN libraries were made using the NEB Ultra II DNA Library Prep Kit for Illumina (NEB #E7645L), and NEBNext Multiplex Oligos for Illumina (96 Unique Dual Index Primer Pairs ...
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bioRxiv - Genomics 2023Quote: Libraries were built on beads using the NEBNext Ul-tra II DNA Library Prep kit (NEB, Cat # E7645L). Biotin pull down was initiated py pipetting 20 µL Dynabeads My-One Streptavidin T1 beads (ThermoFisher Scientific ...
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bioRxiv - Cancer Biology 2022Quote: ... Libraries were prepared using the NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (New England Biolabs). Concentrations of libraries were measured with Qubit dsDNA HS Assay Kit (Thermo Fisher Scientific ...
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bioRxiv - Systems Biology 2023Quote: ... Library preparation was completed using NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (New England Biolabs) and sequenced using MiSeq v3 Paired End 150 bp (Illumina) ...
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bioRxiv - Immunology 2023Quote: Site-directed mutagenesis of Unc93b1 was performed with the Q5 Site Directed Mutagenesis Kit (E0552S, New England Biolabs) according to the manufacturer’s instructions.
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bioRxiv - Genomics 2023Quote: ... Sequencing libraries were prepared using the NEBNext Ultra II RNA Library Preparation Kit for Illumina (New England BioLabs), according to the manufacturer’s instructions ...
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bioRxiv - Genomics 2023Quote: ... cahirinus animal using a Monarch HMW DNA Extraction Kit for Cells & Blood (T3050, New England Biolabs, Ipswich MA) following the manufacturer’s recommended protocol ...
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bioRxiv - Plant Biology 2023Quote: ... IP protocol described in (11) was followed thereafter and Epimark® N6-Methyladenosine Enrichment Kit (New England Biolabs) was used ...
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bioRxiv - Bioengineering 2023Quote: ... GLuc was inserted into the digested backbone through Gibson Assembly Hi-Fi kit (New England Biolabs, Ipswich, MA). AAV-hSyn-hM3Dq-RAM-d2tTA was constructed as previously described19 ...
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bioRxiv - Cell Biology 2023Quote: ... 1ng pcDNA3.3-OCT4 was added for the amplification using the Q5 High-Fidelity DNA Polymerase kit (NEB, M0491). pcDNA3-EGFP (addgene ...
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bioRxiv - Genomics 2023Quote: Purified cDNA was used as an input for the Nextera XT DNA library preparation kit (New England Biolabs). Library quality and size was determined using a TapeStation (Agilent Technologies ...
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bioRxiv - Developmental Biology 2023Quote: ... A library was prepared using the NEBNext Ultra II Directional RNA Library Kit for Illumina (New England Biolabs) following the manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: ... 50 µg of lysostaphin and 100 µl of the tissue lysis buffer (Monarch Genomic DNA purification kit, NEB). Cells were lysed at 37°C for 30 min and then processed following the manufactures instructions.
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bioRxiv - Plant Biology 2023Quote: ... Amplified PCR products with appropriate expected sizes were purified with the Monarch® DNA Gel Extraction Kit (NEB). Purified PCR products were cloned into the pMiniT 2.0 vector (NEB ...