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Citations for New England Biolabs :
4951 - 5000 of 5199 citations for 4 2 Hydroxy 1 Methoxyethyl 1 2 Benzenediol since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Synthetic Biology 2024Quote: ... approximately 1 µg of plasmid DNA was added to a digestion mixture containing 5 µL of rCutSmart Buffer (New England Biolabs, Cat# B6004S), 10-20 units of each restriction enzyme ...
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bioRxiv - Microbiology 2024Quote: ... 1 μg (final volume 100 μL) of the resulting mRNA was incubated with and without RNA 5’-pyrophosphohydrolase (RppH; NEB, 50 U) at 37° C for 1 hour in the supplied 1X buffer (72) ...
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Transcription factors SlMYB41, SlMYB92 and SlWRKY71 regulate gene expression in the tomato exodermisbioRxiv - Plant Biology 2024Quote: ... except for Solyc02g071130.3.1, directly into the destination vector pDLUC15 (Jo et al., 2020) using the NEBuilder® HiFi DNA Assembly Cloning Kit (NEB, England) after digesting it with the enzyme FastDigest HindIII (Thermo Fisher Scientific) ...
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bioRxiv - Molecular Biology 2024Quote: ... SEGS-1 region N was generated by cleaving the Kpn1 site using the Q5-site directed mutagenesis protocol (New England Biolabs, Ipswich, MA) from S1-1.5a (pNSB1829 ...
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bioRxiv - Molecular Biology 2024Quote: ... and joining SEGS-1F and SEGS-1G using the indicated primers in Supplementary Figure 1 and the Q5 high-fidelity DNA polymerase (New England Biolabs, Ipswich, MA). All the primers except for those for site-directed mutagenesis added NotI restriction sites at the 5’ and 3’ends of PCR products ...
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bioRxiv - Synthetic Biology 2024Quote: The reaction was brought up to 50 uL with the addition of CutSmart (final concentration 1X) and 1 uL DpnI (NEB, Catalog #R0176S) and incubated for 1 hour at 37 °C ...
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bioRxiv - Biophysics 2024Quote: ... Transcription templates were prepared by PCR using primers complementary to the T7 promoter and terminator sequences (see Supplementary Table 1) or by digestion of the plasmid DNA with SphI (NEB, Cat# R3182S). mRNA fragments were prepared by in vitro transcription with T7 RNA polymerase using a ratio of 10:30 AU:GC NTPs ...
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bioRxiv - Synthetic Biology 2024Quote: ... The pR and pM probe pools were assembled at a 1:5 vector-to-insert molar ratio using T4 ligase (New England Biolabs, Cat# M0202L) and BbsI-HF (New England Biolabs ...
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bioRxiv - Genomics 2024Quote: ... 50 ng crude digested pcDNA3.1 vector was combined with a 7-times molar excess of crude digested insert and incubated for 30 min at room temperature after addition of 1 µ L Quick Ligase (New England Biolabs, #M2200). NEB stable competent E ...
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bioRxiv - Genetics 2024Quote: DNA from mNPCs in culture were harvested from Kmt2a+/LSL cell line cell line (1 million cells) using Monarch® HMW DNA Extraction Kit (NEB, T3050). We sheared the high molecular weight (HMW ...
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bioRxiv - Genomics 2020Quote: ... CpG dinucleotides were methylated by incubating 1µg of DNA with S-Adenosyl methionine (SAM) (32µM) with CpG Methyltransferase (M.SssI) (4-25 units) (New England BioLabs) at 37°C for 1h before heating to 65°C for 20mins.
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bioRxiv - Genomics 2020Quote: ... by incubating 1µg of DNA with S-Adenosyl methionine (SAM) (32µM) with CpG Methyltransferase (M.SssI) (4-25 units) (New England BioLabs) at 37°C for 1h before heating to 65°C for 20mins.
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bioRxiv - Systems Biology 2020Quote: Single hematopoietic stem and progenitor cells were index-sorted into 384 well plates containing 0.5 µl of nuclease free water with 0.2% Triton-X 100 and 4 U murine RNase Inhibitor (NEB), spun down and frozen at −80°C ...
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bioRxiv - Systems Biology 2021Quote: ... Then a plasmid library was assembled using 4 independent reactions of NEBuilder HiFi DNA Assembly (New England Biolabs) to avoid biases in assembly that might affect the library’s distribution ...
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bioRxiv - Cell Biology 2022Quote: ... pEXKm5 was digested with HindIII and the 4 fragments were assembled via Gibson cloning (New England Biolabs, E2611S).
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bioRxiv - Microbiology 2022Quote: ... DNA was synthesized from extracted RNA using 4 μL LunaScript RT SuperMix 5X (New England Biolabs, NEB, USA) and 8 μL nuclease free water ...
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bioRxiv - Microbiology 2022Quote: ... DNA was synthesized from extracted RNA using 4 μL LunaScript RT SuperMix 5X (New England Biolabs, NEB, USA) and 8 μL nuclease free water ...
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bioRxiv - Neuroscience 2021Quote: ... nuclei were resuspended in 200uL digestion buffer with 4 uL protease (an equal volume of protease K (NEB) was substituted if the manufacturer-provided protease was exhausted ...
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bioRxiv - Molecular Biology 2020Quote: ... the single cell tagmentation product was mixed well with 4 units of Bst 3.0 DNA Polymerase (NEB, Cat.No.M0374) and indexed common primers (Vazyme ...
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bioRxiv - Genomics 2021Quote: ... We then performed a kinase treatment adding 4 μl of T4 Polynucleotide Kinase (PNK, New England BioLabs Inc.), 5 μl of 10X PNK buffer ...
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bioRxiv - Genomics 2021Quote: The beads were magnetically separated and 4 µl of hot PNK mix (0.2 µl PNK [New England Biolabs] ...
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bioRxiv - Genomics 2021Quote: The beads were magnetically separated and resuspended in 20 µl of 3’ end RNA dephosphorylation mixture (4 µl 5x PNK pH 6.5 buffer, 0.5 µl PNK [New England Biolabs; with 3’ phosphatase activity] ...
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bioRxiv - Synthetic Biology 2021Quote: ... annealed by temperature decrease from 95 °C to 4 °C and phosphorylated using T4 Polynucleotide Kinase (NEB, M0201) according to manufactures’ protocol ...
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bioRxiv - Molecular Biology 2021Quote: ... for 12 h at 4 °C with 10 μg of Factor Xa protease (New England Biolabs, Hitchin, UK) per 1 g of mitochondria ...
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bioRxiv - Genomics 2021Quote: ... Nicks were then ligated for 30 min at 37 °C using 4 units of Taq DNA ligase (NEB) in the presence of 0.5 μl thermopol buffer ...
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bioRxiv - Immunology 2021Quote: ... into 384 well plates containing 0.5 µl of nuclease free water with 0.2% Triton-X 100 and 4 U murine RNase Inhibitor (NEB), spun down and frozen at −80°C ...
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bioRxiv - Biophysics 2020Quote: ... SecA(N95) K797C was overexpressed for 4 hours at 37 °C in E.coli NiCo21 cells (New England Biolabs) grown in 2xYT (Acumedia ...
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bioRxiv - Microbiology 2023Quote: ... cDNAs and primers (listed in Table 4) were mixed with Luna Universal qPCR Master mix (New England Biolabs) and amplification was carried out in duplicate in a CFX96 Real-Time System C1000 Touch Thermal Cycler (Bio-Rad ...
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bioRxiv - Neuroscience 2022Quote: ... 4 and subjected to T7E1 digestion in a 20 ul reaction according to the manufacturer’s protocol (NEB cat.M0302). Digestion products were resolved on 8% acrylamide/bis TBE gel and visualized with EtBr.
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bioRxiv - Systems Biology 2024Quote: ... PLP ligation was performed for 4 hours at room temperature using 0.5 U/µl SplintR Ligase (NEB, M0375), 1x Ampligase ligase buffer ...
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bioRxiv - Biophysics 2024Quote: ... A 4°C AKTA Pure FPLC system (Cytiva) was prepared with a 10 mL amylose column (NEB #E8021S) and 2 mL/min flow rate ...
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bioRxiv - Biochemistry 2023Quote: ... and a final concentration of 1.4 nM of hRNase 4 and 0.15 U/μL of polynucleotide kinase (New England Biolabs). The reactions were incubated at 37°C for 1 h and stopped by addition of murine RNase inhibitor to a final concentration of 2 U/μL and incubation at room temperature for 10 minutes ...
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bioRxiv - Genetics 2023Quote: ... The ints-4 sgRNA was inserted into plasmid pDD162 via the Q5 Site-Directed Mutagenesis Kit (NEB, E0554S). The sgRNA plasmid and repair template were Sanger sequenced to confirm correct construct assembly followed by microinjection into EG9882 strain with integrated Cas9 activity (10 ng µl-1 repair template ...
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bioRxiv - Microbiology 2023Quote: ... U6-del-F and U6-del-R (Supplementary table 4) were annealed and phosphorylated using T4 PNK (NEB) and ligated into the restricted plasmid using T7 DNA ligase ...
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bioRxiv - Biophysics 2023Quote: ... The resulting 40 μl of reaction product was mixed with 4 μl of T5 Exonuclease (New England Biolabs) in NEB Buffer 4 ...
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bioRxiv - Developmental Biology 2024Quote: ... The hsp-4 dsRNA was synthesized in vitro with a HiScribe™ T7 RNA Synthesis Kit (NEB, E2050). Subsequently ...
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bioRxiv - Molecular Biology 2024Quote: ... The removal of N-glycans was performed by adding 4 µL of PNGase F (500,000 units/mL, NEB), followed by incubation at 37 °C overnight ...
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bioRxiv - Molecular Biology 2024Quote: ... Plasmids used for in vitro transcription (Supplementary File 4) were restricted digested with PmlI (New England Biolabs R0532L), AfeI (New England Biolabs R0652L) ...
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bioRxiv - Cell Biology 2024Quote: ... The resulting clarified lysate was incubated with 4 mL slurry Amylose beads (New England Biolabs, Ipswich, US-MA) and kept under constant agitation for 2 hours ...
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bioRxiv - Plant Biology 2024Quote: ... Supernatant was recovered after centrifugation (12000 xg, 4°C, 1h) and incubated with 0.002% equilibrated amylose resin (NEB) for 2h on a rotating wheel at 4°C ...
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bioRxiv - Neuroscience 2024Quote: ... Pellets were resuspended in NEB buffer prior to centrifugation at 11,500 rpm for 20 minutes at 4°C on a sucrose gradient (1.6M sucrose in NEB and 0.8M sucrose in NEB). Nuclei-containing pellets were fixed with 0.3% formaldehyde in NEB and rotated at room temperature for 10 minutes ...
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bioRxiv - Evolutionary Biology 2024Quote: ... PCRs were run in 50μl reactions (4 side-by-side replicates) using Q5 enzyme and buffers (NEB; M0491). Amplification was confirmed through agarose gel electrophoresis ...
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bioRxiv - Genetics 2021Quote: ... We isolated poly(A)+ RNA from 1 ng of total RNA using the NEBNext Poly(A) mRNA Magnetic Isolation Module (NEB, Ipswich, MA, USA) following the manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: ... followed by the addition of 5 μl of UDG reaction solution (New England Biolabs, 0.02 U μl−1 UDG in 1X UDG buffer) and an additional 30 min incubation at 37 °C ...
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bioRxiv - Plant Biology 2022Quote: ... Detection of editing in the target genes was performed via amplifying the target regions (primers in Supplemental Table 1) with high fidelity DNA polymerase Q5 (New England Biolabs, Ipswich, MA, USA), followed by cloning of PCR products and sequencing ...
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bioRxiv - Cancer Biology 2022Quote: ... Samples were blocked with 5% BSA in 1X PBS for 30 minutes at room temperature followed by subsequent incubation with primary antibodies against MBP (NEB E8032L, 1:200) and RAD51 (Proteintech 14961-1-AP ...
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bioRxiv - Molecular Biology 2022Quote: ... The reaction was slowly cooled down to room temperature with a Δ -1°C / second gradient and 0.5 μl of each RNase H (NEB, 5 U/μl), RNase T1 (ThermoFisher Scientific ...
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bioRxiv - Genetics 2022Quote: ... mRNA was purified and enriched from 1 µg of total RNA using the NEBNext Poly(A) mRNA Magnetic Isolation Module (New England Biolabs, catalog no. E7490L). RNA fragmentation ...
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bioRxiv - Cell Biology 2022Quote: ... Halt™ Protease and Phosphatase Inhibitor Cocktail in 1X PBS) and then washed twice with either 11 dephosphorylation buffer (1 mM MnCl2, 1X PMP buffer, New England Biolabs, P0753, protease inhibitors) or CN dephosphorylation buffer (50 mM Tris-HCl pH 8 ...
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bioRxiv - Molecular Biology 2020Quote: ... GAL4-Creb5(1-128)T59/T61A and GAL4-Creb5(1-128)C18/C23S were generated by inducing point mutations in the GAL4-Creb5(1-128) vector using the Q5® Site-Directed Mutagenesis Kit (NEB, Cat#: E0554S). The control vector GAL4(1-147 ...