Labshake search
Citations for Illumina :
701 - 750 of 796 citations for Peripheral Blood Mononuclear Cell since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2023Quote: ... A 1.4 nM pool of each QC passed RNA-seq library was sequenced by running a Single Read (SR) 100bp NovaSeq 6000 S2 flow cell on the NovaSeq 6000 Sequencing System (Illumina, Inc.). The fastq files were obtained by demultiplexing the bcl basecall files using the 1.8.4 version bcl2fastq script (Illumina ...
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bioRxiv - Cell Biology 2024Quote: ... libraries were sequenced on a High-output flow cell (400M clusters) using the NextSeq® 500/550 High Output 150 cycles kit (Illumina), in paired- end 76/76 nt mode ...
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bioRxiv - Genomics 2023Quote: ... The pool was sequenced on an entire lane of a NextSeq 500 HO flow cell to generate paired-end 151 bp reads using the NextSeq 500 High Output Kit v2.5 (300 cycles) (Illumina, Cat # 20024908).
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bioRxiv - Microbiology 2023Quote: ... transcriptomic libraries were generated and sequenced on an Illumina NovaSeq 6000 (S4 flow cell, 100bp paired-end; Illumina, San Diego, CA). Raw data is available in the NCBI SRA under the accession number PRJNA857920 ...
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bioRxiv - Genomics 2023Quote: 1 ng of psoralen pull-down DNA (or corresponding Input DNA from the same cells without pull-down) was used to prepare sequencing libraries using a Nextera XT library prep kit (Illumina, Inc) according to the manufacturer protocol ...
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bioRxiv - Genomics 2023Quote: ... Each library was sequenced using 151 base-length read chemistry on a paired-end flow cell on the Illumina NovaSeq6000 (Illumina, USA). After Illumina sequencing ...
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bioRxiv - Neuroscience 2024Quote: ... Sequencing of the 10x Genomics single-cell RNA-seq libraries was performed on an Illumina NovaSeq 6000 (Illumina, San Diego, CA) at the sequencing core facility of the Max Planck Institute for Molecular Genetics (Berlin ...
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bioRxiv - Neuroscience 2024Quote: ... Sequencing of the 10x Genomics single-cell ATAC-seq libraries was performed on an Illumina NovaSeq 6000 (Illumina, San Diego, CA) at by the sequencing core facility at the Max Planck Institute for Molecular Genetics (Berlin ...
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bioRxiv - Microbiology 2024Quote: ... Library preparation (using Ribozero plus) and paired end 50 bp sequencing (on an SP 100 Cycle Flow Cell v1.5 using an Illumina NovaSeq 6000) was conducted by the NYU Langone Genome Technology Center (available for download at) ...
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bioRxiv - Cancer Biology 2024Quote: ... Then the scRNA-seq libraries were constructed by using the Chromium Next GEM Single Cell 3’ Reagent Kits v3 (10X Genomics, USA) and sequenced by using the sequencer Novaseq6000 (Illumina, USA). All procedures were following the manufacturer’s protocol.
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bioRxiv - Genomics 2024Quote: ... The final barcoded library was pooled on a quarter S4 flow cell in 2x150 bp paired-end mode on an Illumina NovaSeq sequencer (Illumina, USA). Hi-C library preparation and sequencing were done at the Norwegian Sequencing Centre (Oslo ...
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bioRxiv - Cancer Biology 2021Quote: ... cells were lysed for 5 min followed by transposase reaction and library amplification using Nextera DNA Library Prep Kit (Illumina, California, USA). Libraries were then size-selected (240-360 bp ...
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bioRxiv - Molecular Biology 2021Quote: ... Barcoded treatment and control replicate libraries were hybridized across four lanes of two S4 flow cells for cBot (Illumina, San Diego, CA) cluster generation and sequencing (paired-end ...
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bioRxiv - Systems Biology 2019Quote: ... denatured in NaOH and combined with HT1 plus a 1% PhiX spike at a final running concentration of 10pM.The flow-cell was clustered using HiSeq PE Cluster Kit v3 (Illumina, PE-401-3001) utilising the Illumina PE HiSeq Cluster Kit V3 cBot recipe V8.0 method on the Illumina cBot ...
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bioRxiv - Cancer Biology 2020Quote: ... MCF10A libraries from three biological replicates of each cell type were prepared using NEBNext® Ultra(tm) RNA Library Prep Kit (Illumina®) and sequenced on HiSeq X by Annoroad Gene Technology Ltd ...
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bioRxiv - Cancer Biology 2020Quote: ... A total of 12 libraries from persistently infected cells and control were generated and sequenced using HiSeq2000 Illumina platform following manufacturer’s instruction (Illumina Inc., San Diego).
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bioRxiv - Molecular Biology 2022Quote: ... The supernatant was discarded and the pellet (cell nuclei) was resuspended in 50 uL transposition mix (50% v/v 2X Tagment DNA (TD) Buffer (Illumina, Cambridge, UK), 32% v/v PBS ...
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bioRxiv - Genomics 2022Quote: ... Samples were then mixed and sequenced to target >20,000 reads per cell for gene expression libraries and >5,000 reads per cell for TCR libraries using NovaSeq S4 or HiSeq 4000 (Illumina. San Diego, CA) with at least 2×100 BP coverage (Extended Data Table 1).
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bioRxiv - Molecular Biology 2020Quote: Next-generation sequencing datasets measured in RNA-Seq experiments with lung cells (GSE147507: Illumina NextSeq 500; GSE146482: Illumina NovaSeq 6000; GSE85121: Illumina HiSeq 2500) were downloaded from NCBI GEO (Table 1 ...
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bioRxiv - Cell Biology 2022Quote: ... All four samples underwent 150 base pair (bp) paired-end read sequencing on one lane of an S4 flow cell (Illumina NovaSeq 6000) to a minimum depth of 500 million reads per sample (Source BioScience ...
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bioRxiv - Immunology 2020Quote: ... 0.1 ng of cDNA from each cell on the plate was individually tagmented using the Nextera XT kit (Illumina, San Diego, CA) in a final volume of 5 µl ...
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bioRxiv - Genetics 2021Quote: ... and prepared bar-coded cDNA libraries for sequencing on an S1 flow cell on the NovaSeq 6000 platform (Illumina, San Diego, CA) as described previously21.
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bioRxiv - Immunology 2020Quote: ... The cell pellets were resuspended in 50 μl transposition mix (25 μl 2X TD buffer, 2.5 μl transposase (Illumina, FC-121-1030), 16.5 μl PBS ...
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bioRxiv - Cell Biology 2021Quote: ... Libraries were diluted to a final concentration of 2.8 pM in HT1 buffer (supplemented with the kit) and loaded on 75-cycle high-output flow cells (Illumina, FC-404-2005) and sequenced on a NextSeq 550 (Illumina) ...
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bioRxiv - Molecular Biology 2020Quote: ... The cell pellet was resuspended in 50 μl transposition mix (25 μl 2X TD buffer, 2.5 μl transposase (Illumina, FC-121-1030), 16.5 μl PBS ...
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bioRxiv - Genetics 2022Quote: ... gDNA of 293T cells with or without transfection was tested by WGS using the Illumina NovaSeq platform (Illumina, San Diego, CA, USA).
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bioRxiv - Physiology 2022Quote: ... Quantified libraries were pooled and sequenced on an Illumina NextSeq using a high capacity 75 read flow cell (Illumina, San Diego, CA) following the manufacturer’s instructions ...
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bioRxiv - Genetics 2022Quote: Libraries are sequenced at between 40,000 and 50,000 fragment reads per cell following Illumina’s standard protocol using the Illumina cBot and HiSeq 3000/4000 PE Cluster Kit (Illumina, San Diego, CA). The flow cells are sequenced as 100 × 2 paired end reads on an Illumina HiSeq 4000 HD using HiSeq 3000 / 4000 sequencing kit and HCS v3.4.0.38 collection software ...
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bioRxiv - Genomics 2023Quote: ... Two biological replicates of 7.5 × 104 cells for each condition were then isolated for direct processing with Nextera Tn5 enzyme (Illumina, FC-131-1096). Samples were treated as previously described (77 ...
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bioRxiv - Bioengineering 2023Quote: ... Libraries were pooled and paired-end sequenced at 100bp per end with an additional 10bp of index reads on a NovaSeq S4 flow cell (Illumina, Novaseq 6000).
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bioRxiv - Genomics 2022Quote: ... we generated a combination of noisy ONT sequencing (MinION R.9.4.1 flow cells with the LSK-109 ligation library prep kit) and Illumina sequencing (PE 150 bp, ∼49x coverage; Illumina NovaSeq 6000). Based on published ONT benchmarking (https://nanoporetech.com/accuracy) ...
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bioRxiv - Microbiology 2023Quote: ... These products were pooled into equal molar proportions and sequenced on Illumina MiSeq V2 standard flow cell (Illumina, San Diego, CA, USA) for 2*300 bp pair-end chemistry according to the manufacturer’s instructions ...
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bioRxiv - Bioengineering 2024Quote: ... The resulting cell nuclei were subjected to incubation for 30 min with Tagment DNA TDE1 Enzyme and Buffer (Illumina, #FC-121-1030). This step fragmented the chromatin and inserted sequencing adapters ...
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bioRxiv - Neuroscience 2024Quote: The Total RNA Library was prepared using the Illumina NovaSeq (S4) 300 cycle flow cell and reagent kit (Illumina, San Diego, CA). The high-throughput sequencing strategy included 300 cycles and a read length of paired-end 150 base pairs ...
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bioRxiv - Cancer Biology 2024Quote: ... Prepared libraries were subjected to high throughput sequencing using Illumina NovaSeq 6000 system using S4 flow cell (Illumina, San Diego, CA, USA) and v1.5 chemistry ...
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bioRxiv - Genomics 2023Quote: HMW DNA from 1.5 x 106 cells was used to generate Illumina libraries with the Nextera XT DNA Library Preparation kit (Illumina FC-131-1024) and Illumina DNA PCR-Free Library Prep ...
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bioRxiv - Cell Biology 2020Quote: ... were prepared from HepG2 cell samples using 200–500 ng of total RNA following the manufacturer’s instructions for the TruSeq RNA kit (Illumina, San Diego, CA, USA). With this kit ...
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bioRxiv - Physiology 2019Quote: ... DNA was amplified using fusion primers designed against the surrounding conserved regions that are tailed with sequences to incorporate flow cell adapters and indexing barcodes (Illumina, San Diego, CA). Each sample was PCR amplified with two differently barcoded V4-V5 fusion primers and were advanced for pooling and sequencing ...
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bioRxiv - Immunology 2021Quote: ... Cells were washed once with PBS before DNA transposition was performed with the Nextera DNA Library Prep Kit (Illumina, Cat. #FC-121-1031). Per sample ...
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bioRxiv - Neuroscience 2020Quote: ... 50bp RNA-seq of IP fractions and total homogenate from 5 replicates per cell type (20 samples total) was performed on a single lane of a HiSeq 3000 (Illumina, San Diego, California), yielding ∼350M reads ...
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bioRxiv - Microbiology 2020Quote: ... The library was then sequenced by Illumina Miseq at Michigan State University (Michigan, USA) using a standard flow cell and 500 cycle v2 reagent cartridge (Illumina Inc., Hayward, California, USA).
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bioRxiv - Systems Biology 2021Quote: Single nuclear 3’ RNA-sequencing was performed at the Indiana University Center for Medical Genomics Core using the Chromium single cell system version 3 (10x Genomics, San Francisco, CA) and the NovaSeq6000 sequencer (Illumina, San Diego, CA). Cell Ranger 4.0 was utilized to generate sample-specific FASTQ files and reads were aligned to the mm10 reference genome using STAR ...
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bioRxiv - Cancer Biology 2020Quote: ... All the cell line mRNA libraries for sequencing were prepared according to the TruSeq Stranded Total RNA Library Prep Kit (Illumina, #RS-122-2201). Sequencing (75 bp ...
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bioRxiv - Developmental Biology 2022Quote: ... Cells were used for the preparation of single-cell libraries using the 10X Genomics Chromium system (10X Genomics, Pleasanton, CA) and sequenced with an Illumina NovaSeq 6000 (Illumina, San Diego, CA) by the KUMC Genome Sequencing Facility (Kansas City ...
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bioRxiv - Developmental Biology 2021Quote: ... Next generation sequencing of equimolar pools of cDNA libraries was performed by loading a paired-end 75bp rapid flow cell to generate a minimum of 25M raw reads on a HiSeq 2500 sequencing platform (Illumina, San Diego, CA). Raw sequencing data (submitted to Gene Expression Omnibus ...
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bioRxiv - Molecular Biology 2022Quote: ... RIP-seq libraries were pooled and sequenced on a MiSeq 50 base pair single-read flow cell using v2 reagents (Illumina, San Diego, CA). SIR libraries were also pooled and sequenced on either a HiSeq2500 high output 100 base pair paired-end flow cell using TruSeq SBS v3 reagents or on a NextSeq500 high output 75 base pair single-read flow cell using v2 reagents (Illumina ...
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bioRxiv - Molecular Biology 2022Quote: ... SIR libraries were also pooled and sequenced on either a HiSeq2500 high output 100 base pair paired-end flow cell using TruSeq SBS v3 reagents or on a NextSeq500 high output 75 base pair single-read flow cell using v2 reagents (Illumina, San Diego, CA).
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bioRxiv - Neuroscience 2020Quote: ... Libraries from 1000-4000 cells (depending on the sample) were sequenced in paired end mode on a HiSeq 4000 instrument (Illumina, San Diego, CA) at an average depth of 174,446 reads per cell (for V2/V3) ...
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bioRxiv - Cancer Biology 2021Quote: ... scRNA-libraries were sequenced on a NextSeq 500/550 High Output Flow Cell v2.5 (150 Cycles) on an Illumina NextSeq 550 (Illumina, San Diego, CA, USA). The bcl2fastq function and the cell ranger (v3.0 ...
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bioRxiv - Immunology 2020Quote: ... University of Edinburgh using the NextSeq 500/550 High-Output v2 (150 cycle) Kit (# FC-404-2002) with a High Out v2.5 Flow Cell on the NextSeq 550 platform (Illumina Inc, #SY-415-1002). 8 libraries were combined in an equimolar pool based on the library quantification results and run across one High-Output Flow Cell ...