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Citations for Illumina :
351 - 400 of 9545 citations for Mouse T cell receptor alpha chain C region TCRA ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2020Quote: ... Libraries were generated using Kapa Biosystems library preparation kit (#KK8201) and multiplexed libraries were sequenced on a 1×75 High output flow cell on the NextSeq550 platform (Illumina). Reads were filtered and trimmed to remove adapter-derived or low-quality bases using Trimmomatic and checked again with FASTQC ...
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bioRxiv - Developmental Biology 2021Quote: ... Chromium Single Cell 3’ v2 single cell RNA-Seq of poly A selected mRNA kit (10X Genomics) and Sequencing was processed on NextSeq 500 (Illumina). Bioinformatics base call by bcl2fastq v ...
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bioRxiv - Immunology 2019Quote: VH and VL libraries from sorted B cell were subjected to NGS on the MiSeq platform with the reagent kit V3 2×300 bp paired-end (Illumina), using an input concentration of 16pM with 5% PhiX.
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bioRxiv - Developmental Biology 2022Quote: 25.000 FACS-sorted cells were collected and used for ATAC Library preparation using Tn5 Transposase from Nextera DNA Sample Preparation Kit (Illumina). The cell pellet was resuspended in 50 µl Lysis/Transposition reaction (12.5 µl THS-TD-Buffer ...
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bioRxiv - Systems Biology 2019Quote: ... The libraries were hybridized to the flow-cell using TruSeq Rapid Duo cBot Sample Loading Kit (Illumina, CT-403-2001), utilising the Illumina RR_TemplateHyb_FirstExt_VR method on the Illumina cBot ...
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bioRxiv - Biochemistry 2019Quote: ... The two cDNA libraries from RNAs of Cfdp1-K1 or dvR2-4 cells were prepared using the TruSeq Stranded mRNA LT Sample Prep Kit (Illumina). Their sequences were obtained using NovaSeq 6000 S4 Reagent Kit by a Nova Sequencing system (S1 Appendix ...
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bioRxiv - Neuroscience 2020Quote: ... cDNA was diluted to an average of 200 pg/µl and 100 pg cDNA from each cell and was tagmented using Nextera XT DNA Library Preparation Kit (Illumina). The tagmentation reaction was incubated at 55 °C for 8 min before removing Tn5 from the DNA by adding 0.5 µl NT buffer per well ...
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bioRxiv - Cell Biology 2021Quote: RNA isolated from colonic epithelial cells was prepared for next-generation sequencing using the stranded RNA library preparation kit with rRNA depletion (Illumina) and sequenced using an Illumina HiSeq 2500 with 100bp single reads ...
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bioRxiv - Cell Biology 2020Quote: ... 50,000 nuclei were purified from WT or Sun dKO cells and DNA was tagmented using the Nextera Library Prep Kit (Illumina). Tagmented DNA was purified using the Qiagen MinElute PCR Purification kit ...
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bioRxiv - Cell Biology 2022Quote: ... The resulting cDNAs from individual cells were used for the construction of NGS libraries with the Nextera XT DNA sample preparation kit (Illumina). Libraries were pooled ...
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bioRxiv - Cell Biology 2022Quote: ... RNA library was constructed with 10x-Genomics Single Cell 3’ RNA sequencing kit v3 as previously described(18) sequenced using a NextSeq500 (Illumina). Cell clusters were characterized using an automated annotation tool(19 ...
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bioRxiv - Developmental Biology 2021Quote: ... Libraries were generated using Kapa Biosystems library preparation kit (#KK8201) and multiplexed libraries were sequenced on a 1×75 flow cell on the HiSeq2000 platform (Illumina). Reads were filtered and trimmed to remove adapter-derived or low-quality bases using Trimmomatic and checked again with FASTQC ...
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bioRxiv - Cancer Biology 2021Quote: Barcoded RNA libraries were prepared starting with 1ug for MCF-10A and 500ng for HCC1806 cell lines of total RNA using the TrueSeq stranded mRNA kit with polyA selection (Illumina), and quantified using a Bioanalyzer DNA 1000 chip (Agilent) ...
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bioRxiv - Molecular Biology 2020Quote: Small RNAs from Aag2 cells (input) or PIWI immunoprecipitations were cloned with the TruSeq small RNA sample preparation kit (Illumina) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2019Quote: ... nuclei were extracted from 50,000 cells per sample and chromatin was tagmented using the TDE1 transposase provided in the Nextera DNA Library Preparation Kit (Illumina). While the original protocol recommended 2.5µL of enzyme ...
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bioRxiv - Genomics 2019Quote: ... Control and NF-YA KD cells were used as input material for the TruSeq Ribo Profile Mammalian Library Prep Kit (Illumina) following the manufacture’s protocol.
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bioRxiv - Genomics 2019Quote: Single cell calling card library preparation was performed using the Nextera Mate Pair Sample Prep Kit (Illumina #FC-132-1001) with modifications to the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2020Quote: ... the post-PCR whole transcriptome amplification (WTA) products from each single cell were transformed into sequencing libraries using a Nextera XT kit (Illumina) and unique 8-bp DNA barcodes ...
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bioRxiv - Immunology 2021Quote: ... The cDNA was enriched for V(D)J cDNA using the Human B cell Chromium Single Cell V(D)J Enrichment Kit (Cat#1000016) followed by library construction to add the priming sites used by Illumina sequencers ...
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bioRxiv - Immunology 2020Quote: ... Single cell RNA libraries were prepared according to the 10x Genomics Chromium Single Cell 3’ Reagent Kits v2 User Guide and sequenced on a HiSeq4000 (Illumina).
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bioRxiv - Genomics 2021Quote: ... cells and CD14+ monocytes were generated using the SureCell WTA 3′ Library Prep Kit for the ddSEQ System (20014280, Illumina) with the following modifications ...
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bioRxiv - Physiology 2020Quote: ... single-cell barcoded cDNA libraries were generated with 10X Chromium preparation system using the Chromium Single Cell 3’ Library & Gel Bead Kit v3 and sequenced by Illumina HiSeq ...
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Enhancer Remodeling Promotes Tumor-Initiating Activity in NRF2-Activated Non-Small Cell Lung CancersbioRxiv - Cancer Biology 2020Quote: ... 4 μg of total RNA from A549 cells was subjected to rRNA removal using the Ribo-Zero Gold kit (Illumina). cDNA sequencing libraries were then prepared using the SureSelect Strand-Specific RNA library preparation kit (Agilent Technologies ...
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bioRxiv - Immunology 2020Quote: ... Libraries were sequenced to obtain more than 20 million 100 × 100 bp paired-end reads (S4 P200 flow cell and sequencing Kit; Illumina) mapping uniquely to mouse mm10 mRNA reference.
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bioRxiv - Immunology 2021Quote: VH libraries from sorted B cells were subjected to NGS on the MiSeq platform with the reagent kit V3 2 × 300 bp paired-end (Illumina), using an input concentration of 16 pM with 5% PhiX ...
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bioRxiv - Immunology 2020Quote: ... Single cell libraries from sorted samples were generated using a UMI-based droplet-partitioning platform with the Chromium single cell 3’ library and gel bead kit v2 and v3 (10X Genomics) and then sequenced using a NovaSeq S2 (Illumina). Technical information relevant to the scRNA-Seq samples is found in Table S3.
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bioRxiv - Microbiology 2021Quote: ... The library pool was then loaded into flow cell of MiSeq Reagent Kit V2 (2 × 150 cycles) and sequenced in a MiSeq platform (Illumina), according to the manufacturer’s instructions ...
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bioRxiv - Genomics 2021Quote: Single-cell libraries were prepared from 12,000 cells per time point (Figure 1A) using the SureCell WTA 3’ Library Prep Kit for the ddSEQ System (Illumina). Cells with viability >90% were collected by detaching cells from plates using Trypsin-EDTA 0.25% ...
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bioRxiv - Genetics 2020Quote: ... Single-cell library was pooled with equal volumes and sequenced using Nextseq500/550 High Output Kit v2.5 (Illumina, cat# 20024906) on Nextseq500/550 sequencer (Illumina ...
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bioRxiv - Cancer Biology 2022Quote: ... equal amount of RNA from each sample or cell types were used to generate the library using the TruSeq RNA Sample Prep Kit v.2 (Illumina). Paired-end sequencing was performed using TruSeq 3000 4000 SBS Kit v.3 (Illumina ...
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bioRxiv - Neuroscience 2022Quote: ... was used to process cell lysates to reverse transcription and library preparation was performed using the Nextera DNA Sample Preparation kit (Illumina) following the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2022Quote: ... RNA from the barcoded cells was subsequently reverse-transcribed and sequencing libraries constructed with reagents from a Chromium Single Cell 3’ v3 reagent kit (10x Genomics) and sequenced with the NovaSeq system (Illumina).
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bioRxiv - Evolutionary Biology 2022Quote: ... Each pool was sequenced 100 bp single-end on two flow cells of a HiSeq2500 sequencer using the HiSeq SBS sequencing kit version 4 (Illumina). Sequencing was conducted in two separate HiSeq runs to yield enough coverage per sample and later combined ...
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bioRxiv - Developmental Biology 2022Quote: ... Barcoded cDNA libraries were multiplexed onto a TruSeq paired-end flow cell and sequenced (100-bp paired-end reads) with a TruSeq 200-cycle SBS kit (Illumina). Samples were run on an Illumina NovaSeq 6000 sequencer at the KUMC Genome Sequencing Facility ...
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bioRxiv - Microbiology 2022Quote: ... ATAC-seq libraries were prepared from 50,000 cells per replicate using Illumina Tagment DNA Enzyme and Buffer Kit (Illumina 20034197) according to the Omi-ATAC-seq protocol (Corces et al. ...
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bioRxiv - Systems Biology 2022Quote: We sequenced all our single-cell libraries using a NextSeq 500 with the High Output Kit v2.5 (75 cycles, Illumina, 20024906). For samples sequenced with the Single Cell 3’ Reagent Kit V3 (single index) ...
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bioRxiv - Developmental Biology 2022Quote: ... Indexed libraries were prepared using the Chromium Single Cell 3’ Library & Gel Bead Kit v2 (10X Genomics; PN-120237) and sequenced on a NovaSeq 6000 (Illumina) to obtain a sequencing depth of 1.50E+08 paired-end reads per library ...
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bioRxiv - Molecular Biology 2023Quote: The ChIP libraries were sequenced on a High-output flow cell (400M clusters) using the NextSeq® 500/550 High Output v2.5 150 cycles kit (Illumina), in paired-end 75/75nt mode ...
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bioRxiv - Microbiology 2023Quote: ... Eighteen pM libraries were clustered in a high-output flow cell using HiSeq Cluster Kit v4 on a cBot (Illumina). After cluster generation ...
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bioRxiv - Cancer Biology 2023Quote: ... with library preparation using the Chromium Next GEM Single Cell 3’ Kit (10x Genomics, PN-1000268) and sequencing was performed on the NovaSeq 6000 (Illumina) by the WSU GSC ...
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bioRxiv - Physiology 2023Quote: ... The pooled libraries were diluted to 380 pM prior to onboard clonal clustering of the patterned flow cell using the NovaSeq 6000 S1 Reagent Kit v1.5 (200 cycle) (Illumina 20028318). A 2×101 cycle sequencing profile with dual index reads is completed using the following sequence profile ...
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bioRxiv - Cell Biology 2022Quote: Libraries from primary cardiac macrophages and total myocardium as well as Schlafenlnc-deficient and control cell lines were generated using the TruSeq Stranded RNA Kit (Illumina) according to the manufacturer’s protocol ...
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bioRxiv - Developmental Biology 2023Quote: ... 100 cycle sequencing kit (v1.5) to a minimum depth of 50K reads per cell (Illumina Inc., San Diego, CA, USA). Base calling was done by Illumina RTA3 and output was demultiplexed and converted to FastQ format with Cell Ranger (10X Genomics ...
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bioRxiv - Physiology 2023Quote: ... Eighteen pM libraries were clustered in a high-output flow cell using HiSeq Cluster Kit v4 on a cBot (Illumina). After cluster generation ...
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bioRxiv - Cell Biology 2023Quote: ... Illumina sequencing libraries were indexed with unique 5’ and 3’ barcode combinations (up to 384 cells) using the Nextera XT DNA library preparation kit (Illumina). Libraries were pooled and size-selected with 0.9X AmpPure XP beads ...
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bioRxiv - Cancer Biology 2023Quote: ... NEBNext Low Input RNA libraries were prepared manually following the manufacturer’s protocol (NEBNext® Single Cell/Low Input RNA Library Prep Kit for Illumina® Instruction Manual Version 5.0_5/20 ...
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bioRxiv - Microbiology 2023Quote: ... Cells were processed using the Chromium Next GEM Single Cell 3’ Reagent Kits (10x genomics) and sequenced on a NovaSeq 6000 (Illumina) at the Harvard Medical School Biopolymers Core Facility as 28 (read 1 ...
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bioRxiv - Microbiology 2023Quote: ... then diluted to 150 pM in elution buffer supplied in the library prep kit and loaded to the iSeq100 flow cell for sequencing on iSeq100 (Illumina). After the NGS run ...
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bioRxiv - Neuroscience 2023Quote: ... after which onboard clonal clustering of the patterned flow cell was performed using a NovaSeq 6000 S1 Reagent Kit v1.5 (200 cycle) (Illumina 20028318). A 2x101 cycle sequencing profile with dual index reads was completed using the following sequence profile ...
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bioRxiv - Neuroscience 2023Quote: ... Eighteen pM libraries were clustered in a high-output flow cell using HiSeq Cluster Kit v4 on a cBot (Illumina). After cluster generation ...