Labshake search
Citations for Illumina :
651 - 700 of 752 citations for IL 1 Alpha Human since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2021Quote: ... 1 ug of treated RNA was then input into the Illumina TruSeq Small RNA Library Preparation Kit (Illumina; Cat #: RS-200-0012) with 11 cycles of PCR amplification ...
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bioRxiv - Genomics 2020Quote: ... Samples were prepared for sequencing using 1 ug of genomic DNA following the TruSeq PCR free kit protocol (Illumina, FC-121-3001). Resulting libraries were quality checked on an Agilent DNA 1000 bioanalyzer (Agilent Technologies ...
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bioRxiv - Genomics 2019Quote: ... 500bp and 800bp) and long insert mate-pair reads (4-6Kb, 8-10Kb and 1-20Kb) were sequenced using HiSeq 2500 (Illumina Inc., USA) at SciGenom Labs ...
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bioRxiv - Genomics 2019Quote: TruSeq libraries were prepared with 1 µg of total RNA using the TruSeq Stranded mRNA Library Prep Kit (Illumina, #RS-122-2101) and following the manufacturer's instructions ...
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bioRxiv - Plant Biology 2020Quote: 42 RNA samples from experiment 1 were used for mRNA-Seq library preparation using the TruSeq Stranded mRNA Library Prep Kit (Illumina, CA, USA). Libraries were multiplexed and single-end 100 nt RNA-Seq was performed on a Novaseq 6000 at the GIGA Center (University of Liege ...
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bioRxiv - Genomics 2022Quote: ... a library was prepared from 1 μg of genomic DNA and a TruSeq DNA PCR-free Sample Preparation Kit (Illumina, CA, USA). Paired-end (151 bp per read ...
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bioRxiv - Genomics 2022Quote: ... Libraries were prepared with 1 μg of total RNA for each sample using a TruSeq Stranded mRNA Sample Prep Kit (Illumina, CA, USA). Paired-end sequencing (151 bp ...
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bioRxiv - Microbiology 2022Quote: ... The V4 region of the 16S rRNA gene was amplified from 1 or 7μl DNA and sequenced with a MiSeq (Illumina, San Diego, CA) using the 500 cycle MiSeq Reagent Kit ...
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bioRxiv - Cell Biology 2020Quote: ... cDNA was diluted 1:15 and subsequently tagmented and amplified for 12 cycles using the Nextera XT DNA Library Preparation Kit (Illumina FC-131). Individual libraries were QC’d by Qubit and Agilent Bioanalyzer ...
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bioRxiv - Genomics 2019Quote: ... libraries were prepared according to the manufacturer’s instructions with a 1% spike-in of the ϕX174 control library (Illumina #FC-110-3002) and sequenced on an Illumina NextSeq 500 instrument with a High Output v2 reagent kit (Illumina #FC-404-2005) ...
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bioRxiv - Genetics 2020Quote: ... One sequencing experiment (Experiment 1) was performed according to the manufacturer’s directions for the TruSeq Small RNA Sample Preparation Kit (Illumina, RS-200-0012). The protocol was adapted to incorporate a 2S blocking DNA oligo for removal of prevalent Drosophila small ribosomal RNA from the sequencing library [67] ...
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bioRxiv - Genomics 2020Quote: ... The cDNA library was generated from 1 µg of total RNA using TruSeq RNA Sample Prep Kit (Illumina, San Diego, CA, USA) following manufacturer’s protocols ...
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bioRxiv - Immunology 2020Quote: ... The Illumina RNA-seq cDNA library was prepared using 1 μg of RNA from each sample and TruSeq V2 kits (Illumina, CA, USA), with reduced RNA fragmentation time (3 minutes ...
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bioRxiv - Plant Biology 2021Quote: ... Libraries for RNA-Seq were prepared from 1 µg of total RNAs with the TruSeq Stranded mRNA Library Prep Kit (Illumina, CA, USA), multiplexed and sequenced in two runs with an Illumina NextSeq500 device (high throughput mode ...
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bioRxiv - Immunology 2022Quote: ... Amplified (1 ng) cDNA was used to generate barcoded libraries using the Nextera XT DNA library preparation kit (Illumina, San Diego, CA). The size of the libraries was checked using the Agilent 2100 Bioanalyzer High Sensitivity DNA chip and their concentration was determined using the Qubit® fluorometer (ThermoFisher Scientific ...
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bioRxiv - Molecular Biology 2022Quote: ... 1 µg of total RNA was used as input for the TruSeq RNA Library Prep Kit v2 (Illumina, Inc., San Diego, CA) and the manufacturer’s protocol was followed ...
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bioRxiv - Genomics 2023Quote: 1 ng of genomic DNA from each sample was used with Illumina MiSeq Nextera XT DNA Library Preparation Kit (Illumina, Inc., USA), the paired-end library was constructed with an insert (500 bp ...
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bioRxiv - Systems Biology 2023Quote: ... 0.5 µL of universal primer and 0.5 µL of index primer (each at 0.5µM concentration; NEBNext multiplex oligos for Illumina index primers set 1 E7335). This mix has been amplified for 15 cycles (same amplification conditions as before) ...
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bioRxiv - Genomics 2023Quote: ... Libraries were prepared using the QuantSeq 3’ mRNA-Seq Library Prep Kit FWD for Illumina and UMI Second Strand Synthesis Module for QuantSeq FWD (Illumina, Read 1) from Lexogen (015.96 and 081.96 ...
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bioRxiv - Immunology 2023Quote: ... and sequencing was performed in a 1×100 bp single-read format using a NovaSeq 6000 v1.5 100-cycle reagent kit (Illumina, Cat no. 20028316). Base calling was performed with Illumina Real Time Analysis (RTA ...
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bioRxiv - Evolutionary Biology 2023Quote: ... and g2997a) at the forward primer binding site used in the ‘round 1 PCR’ step below (the step attaching partial Illumina sequencing adaptors), thus excluding PCR amplification of the competitor viruses during sequencing library preparation such that only the library viruses ...
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bioRxiv - Genomics 2022Quote: ... we generated a combination of noisy ONT sequencing (MinION R.9.4.1 flow cells with the LSK-109 ligation library prep kit) and Illumina sequencing (PE 150 bp, ∼49x coverage; Illumina NovaSeq 6000). Based on published ONT benchmarking (https://nanoporetech.com/accuracy) ...
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Lysyl oxidase regulates epithelial differentiation and barrier integrity in eosinophilic esophagitisbioRxiv - Cell Biology 2023Quote: ... Sequencing libraries were constructed from total RNA (1 μg) using a TruSeq Stranded mRNA Library Prep (Illumina Inc., San Diego, CA, USA). RNA sequencing was performed on Illumina HiSeq2000 platform ...
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bioRxiv - Neuroscience 2023Quote: ... Female and male samples after MR1 were sequenced (single-end reads, 1×75 nt) with Illumina NextSeq 500 (Illumina, San Diego, USA). Female samples after MR2 were sequenced (single-end reads ...
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bioRxiv - Genetics 2023Quote: ... 10 ng of amplicon DNA from PCR reaction 1 was combined with 10 µL of Nextera adapter index primers (Illumina Cat#20027213) and 25 µL Kapa HiFi Hotstart ReadyMix ...
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bioRxiv - Genomics 2024Quote: DNA concentration was normalized to 1 ng/μl and subjected to library preparation using Nextera XT DNA Library Preparation Kit (Illumina, CA, USA) according to the manufacturer’s instructions and sequenced with NextSeq550 using Mid Output Kit v2.5 (300 cycles ...
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bioRxiv - Neuroscience 2024Quote: ... Illumina Sequencing libraries were prepared using the TruSeq low sample protocol from 1 µg of total RNA (Illumina, San Diego, CA, USA). The resulting libraries were sequenced in paired-end mode ...
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bioRxiv - Evolutionary Biology 2021Quote: ... A paired-end library was constructed from 1 µg of the DNA using TruSeq DNA PCR-Free LT Sample Prep kits (Illumina K.K., Tokyo, Japan) following the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2020Quote: ... libraries were prepared from 1 ng of cDNA using the Nextera® XT DNA Library Preparation Kit (Illumina Inc., San Diego, CA USA) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2021Quote: ... and 2.5 µl of each Nextera Index 1 (N7XX) and Nextera Index 2 (N5XX) primers from a Nextera DNA Sample Preparation Index Kit (Illumina, FC-121-1011). PCR was performed according to the following protocol ...
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bioRxiv - Genetics 2020Quote: ... 5 males and 1 female) were used to perform library preparation and sequencing (100 bp single-end) on the HiSeq 4000 sequencer (Illumina, San Diego, CA) following the manufacturer’s sequencing protocols ...
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bioRxiv - Systems Biology 2019Quote: ... six-base indexes distinguish samples and allow multiplexed sequencing and analysis using 48 unique indexes ((Set A: indexes 1-12 (Illumina, RS-200-0012), Set B ...
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bioRxiv - Evolutionary Biology 2019Quote: ... Sequencing was carried out in a 1×50bp single end format using Illumina HiSeq 4000 SBS reagents (Illumina Inc., San Diego, California, USA). Base calling was done by Illumina Real Time Analysis (RTA ...
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bioRxiv - Molecular Biology 2019Quote: ... A RIP-seq complementary DNA library was prepared from 1 µg of total RNA using the TruSeq RNA Library Prep Kit v2 (Illumina, San Diego, CA), according to the manufacturer’s protocol ...
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bioRxiv - Immunology 2021Quote: ... Chromatin fragments bound to magnetic beads were resuspended in 30 μl Tagmentation mix (14 μl H2O, 15 μl Tagment DNA Buffer [Illumina, order no. 15027866], 1 μl Tagment DNA Enzyme [Illumina, order no. 15027865]) and incubated for 10 minutes at 37°C ...
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bioRxiv - Genomics 2021Quote: RNA-Seq library preparation was carried out from 1 µg total RNA using the TruSeq Stranded mRNA kit (Illumina, San Diego, CA, USA), which allows mRNA strand orientation ...
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bioRxiv - Cell Biology 2022Quote: ... the sequencing was performed under the setting of single read 1×51 bp to generate ∼30 million reads per sample on HiSeq 1000 sequencer (Illumina, San Diego, CA). Data analysis was performed as described before (56).
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bioRxiv - Neuroscience 2021Quote: ... ensuring that all sequencing adapters were removed and that the average quality score for each read was above Q20 (representing 1 in 100 Illumina base error rate). Reads which were aligned by HISAT2 v2.0.6 (Kim et al. ...
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bioRxiv - Cancer Biology 2020Quote: ... Libraries were pooled and single end-sequenced (1×75) on the Illumina NextSeq 500 using the High output V2 kit (Illumina, San Diego, CA). Quality control was performed using Fastqc (v0.11.5 ...
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bioRxiv - Neuroscience 2022Quote: ... of the three offspring and their parents was performed at the University of Washington with 1 μg of DNA on a HiSeq 2000 Sequencing System (Illumina, San Diego, CA). The WGS data were aligned using a standard BWA pipeline to the Budgerigar (Melopsittacus undulatus ...
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bioRxiv - Evolutionary Biology 2022Quote: ... We sent 100 ng of DNA from 289 sapsuckers (Supplemental Materials Table 1) to Genome Quebec for sequencing on either an Illumina HiSeq4000 PE150 or the NovaSeqSP 6000 PE150 (Table S1) (Illumina, San Diego, CA).
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bioRxiv - Genomics 2019Quote: ... libraries were prepared according to the manufacturer’s instructions with a 1% ϕX174 spike-in and sequenced on an Illumina MiSeq instrument with a Reagent Kit v3 (Illumina #MS-102-3001), reading 169 nt for read 1 and 6 nt for the P7 index read.
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bioRxiv - Genomics 2019Quote: ... 114 experimental lines selected form the University of Nebraska soybean breeding program (Supplementary Table 1) were sequenced to a depth of 1X or greater on an Illumina NextSeq 500 (Illumina Hayward, Hayward, CA) using the manufacturer’s protocol and 150 base pair paired end reads ...
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bioRxiv - Genomics 2021Quote: RNA-Seq library preparation was carried out from 1 µg total RNA using the TruSeq Stranded mRNA kit (Illumina, San Diego, CA, USA), which allows mRNA strand orientation (sequence reads occur in the same orientation as anti-sense RNA) ...
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bioRxiv - Evolutionary Biology 2021Quote: ... A paired-end library was constructed from 1 µg of the DNA using TruSeq DNA PCR-Free LT Sample Prep kits (Illumina K.K., Tokyo, Japan) following the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2021Quote: ... RNA-seq libraries were generated with 1 μg of RNA as input using the TruSeq RNA Sample Prep Kit (Illumina, San Diego, CA) with poly(A)-enrichment according to the TruSeq protocol ...
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bioRxiv - Genomics 2020Quote: ... 1 nmol of Nimblegen HE universal blocking oligonucleotides and 1 nmol of Nimblegen HE index-specific blocking oligonucleotides (corresponding to the Illumina TS index used) in a 1.5 μl microcentrifuge tube ...
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bioRxiv - Genomics 2020Quote: ... We spiked 12 pM library with 1% PhiX control (PhiX Control v3) and sequenced on an Illumina MiSeq platform (Illumina, San Diego, USA) using a MiSeq Nano Reagent Kit v2 (500 cycles) ...
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bioRxiv - Genomics 2020Quote: ... We spiked the 12pM library with 1% PhiX control (PhiX Control v3) and sequenced on an Illumina MiSeq platform (Illumina, San Diego, USA) using a MiSeq Nano Reagent Kit v2 (500 cycles).
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bioRxiv - Microbiology 2022Quote: Sequencing libraries were prepared from 1 ng of each VSG PCR product using the Nextera XT DNA Library Preparation Kit (Illumina, FC-131-1096) following the manufacturer’s protocol except for the final cleanup step ...