Labshake search
Citations for Illumina :
451 - 500 of 752 citations for IL 1 Alpha Human since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genetics 2021Quote: ... Sequences were demultiplexed using QIIME2 (core distribution 2018.6)42 for dataset 1 and BCL2FASTQ (Illumina, San Deigo, CA) for dataset 2 ...
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bioRxiv - Microbiology 2021Quote: ... each 1 μL of 10 μM forward and reverse primers from Nextera XT Index Kit v2 (Illumina, USA), 8 μL of nuclease-free water ...
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bioRxiv - Molecular Biology 2022Quote: ... Each library was sequenced in 1 × 75 bp of single read mode using a NextSeq 500 platform (Illumina). Adapter sequences are removed from sequencing reads using Trim Galore (version 0.6.7 ...
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bioRxiv - Microbiology 2022Quote: ... 2.5 µL primer P5 (5 µM) and 2.5 µL Index 1 primer (N7**, Illumina, CN FC-131-2001) was used for the PCR enrichment ...
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bioRxiv - Systems Biology 2022Quote: ... 1 ng of cDNA was “tagmented” and bar coded using a Nextera XT DNA Sample Preparation Kit (Illumina). The final libraries were purified using AmPure XP magnetic beads (Beckman Coulter ...
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bioRxiv - Molecular Biology 2023Quote: ... A 2 µl aliquot of the denatured library pool is then added to 1 ml of HT1 (Illumina), mixed and 120 µl is added to the ‘sample library’ cBot strip tube ...
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bioRxiv - Neuroscience 2023Quote: ... 1 μg of DNA was used to generate sequencing library using the mRNA-Seq Sample Preparation Kit (Illumina) and sequenced on an Illumina Hiseq platform (Novagene ...
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bioRxiv - Developmental Biology 2023Quote: ... Isolated nuclei were mixed with 50 µl of transposition reaction mix containing Tagment DNA Enzyme 1 (Illumina, 20034197). Samples were incubated using a thermo shaker at 37°C ...
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bioRxiv - Molecular Biology 2023Quote: ... minus (Table 1) was used for whole-genome sequencing using Illumina short-read sequencing technology (Illumina, California, USA). Paired-end libraries with insert sizes of 327–353 bp were sequenced on a NovaSeq 6000 system (Illumina).
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bioRxiv - Genomics 2023Quote: ... indexed using IDT for Illumina Nextera UD indexes Sets 1–4 (384 Indexes, Cat no: 20043137, Illumina, USA) and products were amplified ...
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bioRxiv - Evolutionary Biology 2023Quote: ... We generated Illumina cDNA libraries from 1 μg of purified RNA using KAPA Stranded mRNA-Seq Kit (Illumina), and uniquely indexed libraries using unique dual indexes (Illumina) ...
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bioRxiv - Plant Biology 2024Quote: ... prior to generating individual indexed libraries each from 1 μg RNA using the Stranded mRNA Prep kit (Illumina), as recommended ...
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bioRxiv - Neuroscience 2023Quote: ... 1 ng of amplified RNA was used to prepare cDNA libraries (Nextera XT DNA library preparation kit; Illumina). cDNA libraries for 4 biological replicates for both control (THD’GAL4/+ ...
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bioRxiv - Cell Biology 2020Quote: ... Whole genome sequencing was performed with 1 μg of the digested DNA using a HiSeq X Ten Sequencer (Illumina) at Macrogen (South Korea).
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bioRxiv - Developmental Biology 2021Quote: ... Sequencing libraries were prepared from 1 μg total RNA using the TruSeq Standard mRNA LT Sample Preparation Kit (Illumina) and sequenced by Illumina NextSeq500 (Illumina ...
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bioRxiv - Evolutionary Biology 2021Quote: ... eurysternus samples (complete metadata including barcodes are available in Supplementary data 1. The purified library was sequenced on Illumina Miseq using V2 chemistry with 500 cycles (Norwegian High Throughput Sequencing Centre ...
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bioRxiv - Cancer Biology 2019Quote: ... Sequencing was performed on an Illumina NovaSeq platform (paired-end, Read 1: 26 cycles and Read 2: 98 cycles) according to the manufacturer’s instructions (Illumina).
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bioRxiv - Physiology 2020Quote: Sequencing of the library was performed at a final concentration of 1.8 pM and with a 1 % PhiX v3 control library spike-in (Illumina) on the NextSeq500 sequencing system (Illumina) ...
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bioRxiv - Genetics 2019Quote: ... We tagmented 5 ng of cDNA using 1 uL Nextera Tagment DNA Tn5 transposase (Illumina, San Diego, CA, 15027916) in a 10 uL tagmentation mix for 10 minutes at 55 °C.
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bioRxiv - Microbiology 2019Quote: ... 1 µg purified total RNA was used as input for the Truseq stranded total RNA Gold library prep (Illumina). Ribosomal RNA was depleted using Ribo-Zero H/M/R Gold and depleted RNA fragmented for 8min at 94C ...
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Enterobacter sp. SA187 mediates plant thermotolerance by chromatin modification of heat stress genesbioRxiv - Plant Biology 2020Quote: We performed mRNA libraries with 1 µg of total plant RNA using a stranded mRNA Library Prep kit (Illumina). Pooled libraries were sequenced using Illumina HiSeq 4000 platform which resulted in paired-end reads of length 151 bps ...
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bioRxiv - Molecular Biology 2019Quote: ... Initial RNA-seq libraries were made using the TruSeq RNA prep kit by Illumina (shW, shSv210-1, shSv210-2). A second set of libraries from shW ...
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bioRxiv - Genomics 2019Quote: ... and the libraries were prepared from 1 µg of RNA using TruSeq RNA Library Prep (Illumina, San Diego, CA). These samples were sequenced on a Illumina HiSeq 2000 lane as 75 bp single ends ...
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bioRxiv - Genetics 2020Quote: ... were incubated in a 50 µl reaction with 0.8 µl of Tn5 transposase at 1× TD buffer from the Nextera library preparation kit (Illumina) for 5 min at 55°C ...
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bioRxiv - Genomics 2021Quote: ... Complementary DNA (cDNA) libraries were prepared from 1 μg RNA using TrueSeq RNA Sample Prep Kit (Illumina, California, USA), according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2021Quote: ... adding Illumina sequencing adapters and dual-index barcodes (index 1(i7) and index 2(i5)) (Illumina, San Diego, CA) to the amplicon target ...
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bioRxiv - Bioengineering 2022Quote: ... and 1 ×107 cells from each gate were barcoded and the sequences for each cell were determined by Illumina next-generation sequencing15 ...
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bioRxiv - Genomics 2022Quote: Combined with four types of libraries constructed: (1) the short-read sequencing library with 350-bp insertions from Illumina HiSeq X Ten instrument platforms ...
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bioRxiv - Immunology 2020Quote: ... Libraries were sequenced on the iSeq (cases 1 - 6 and controls) or NovaSeq 6000 (case 7 and controls) (Illumina) using 150nt paired-end reads.
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bioRxiv - Cell Biology 2021Quote: ... Samples were sequenced according to the Illumina user manual with 80 cycles of read 1 (forward) using the NextSeq 500/550 High Output Kit v2.5 (75 Cycles) (Illumina) with the 20% PhiX spike in Illumina PhiX control kit (Illumina).
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bioRxiv - Microbiology 2021Quote: ... All cDNA libraries were sequenced on an Illumina NextSeq 500 instrument at the Boston University microarray and sequencing core according to Illumina and 10x Genomics guidelines with 1.4-1.8pM input and 1% PhiX control library spike-in (Illumina, USA).
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bioRxiv - Molecular Biology 2022Quote: ... The chromatin was then tagmented by resuspending beads in 29 µl Tagmentation Buffer (10 mM Tris-HCl pH 8.0, 5 mM MgCl2, 10% dimethylformamide) and adding 1 µl of transposase (Illumina). Samples were incubated at 37 °C for 10 min and the reaction was terminated by adding 150 µl RIPA buffer ...
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bioRxiv - Genetics 2022Quote: ... sequence reads were mapped to the mouse reference genome GRCm38/mm10 (iGenomes, Illumina; chromosomes 1-19, X, Y, M) using the Rsubread v1.28.1 package in R v3.4.4 ...
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bioRxiv - Pathology 2019Quote: ... NGS libraries were prepared from 1 ng of extracted DNA using Nextera®XT DNA Sample Preparation Kit (Illumina), and sequencing was performed on an Illumina MiSeq sequencer using MiSeq Reagent Kit v2 (500-cycles ...
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bioRxiv - Cell Biology 2019Quote: ... cDNA libraries were prepared using 1 µg of total RNA using the TruSeq RNA Sample Preparation Kit v2 (Illumina). RNA quality was assessed by an Agilent Bioanalyzer 2100 ...
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bioRxiv - Systems Biology 2019Quote: ... Washed AMPure XP beads attached to double-stranded cDNAs were directly eluted using 3.75 μL of 1× diluted Tagment DNA Buffer (Illumina) and mixed well using a vortex mixer and pipetting ...
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bioRxiv - Genetics 2020Quote: ... for PPI (see supplementary methods).14 The 148 additionally selected SNV markers (supplementary table 1) were genotyped in 1,012 F2 mice by Illumina BeadArray genotyping (Illumina Golden Gate assay ...
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bioRxiv - Synthetic Biology 2021Quote: ... cDNA was diluted to an average of 200 pg/µl and 100 pg cDNA from each cell was tagmented by adding 1 µl TD and 0.5 µl ATM from a Nextera XT DNA Library Preparation Kit (Illumina) to 0.5 µl diluted cDNA in each well of a fresh 384-well plate ...
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bioRxiv - Immunology 2020Quote: ... RNA-Seq libraries were prepared using 1 μg of end-repaired cDNA using the TruSeq Stranded RNA Kit (Illumina), however due to the method of amplification the libraries were not stranded ...
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bioRxiv - Cancer Biology 2019Quote: ... Paired-end sequencing libraries were prepared using 1 μg of purified RNA following the mRNA-Seq Sample Prep Kit according to the manufacturer’s instructions (Illumina). RNA-Seq libraries were sequenced on two lanes each of an Illumina GAIIx Genome Analyser to a minimum depth of 49 million reads ...
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bioRxiv - Genomics 2021Quote: ... 30 μL of NEBNext Ultra II Ligation Master Mix, 1 μL of NEBNext Ligation Enhancer, 2.5 μL of NEBNext Adapter for Illumina) at 20 °C for 15 min. ...
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bioRxiv - Developmental Biology 2022Quote: ... The PCR product was purified in a 1 % agarose gel and sequenced using a NextSeq 550 and NovaSeq (Illumina) to obtain 79+79-nt ...
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bioRxiv - Plant Biology 2022Quote: The triplicate total RNA samples used for single-end 1×75bp RNA-Seq on a NextSeq500 sequencing platform (Illumina). After examining the data quality using FastQC (v0.11.5) ...
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bioRxiv - Genomics 2022Quote: ... 5 μl of Index primer (both provided in NEBNext Multiplex Oligos for Illumina Index Primers Sets 1 and 2), and 25 μl NEBNext Ultra II Q5 Master Mix (PCR cycling conditions ...
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bioRxiv - Plant Biology 2023Quote: ... The library was diluted to 0.5 nM with EB in 18 μL and spiked with 1% PhiX Control v3 (Illumina) before being prepared for loading using a NovaSeq XP 2 lane kit v1.5 (20043130 ...
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bioRxiv - Microbiology 2022Quote: ... RNA-Seq libraries were prepared using 1 ug of total RNA with TruSeq Stranded mRNA Library Prep kit (Illumina) following the manufacturer’s instructions ...
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bioRxiv - Pathology 2023Quote: ... All cDNA libraries were sequenced on an Illumina NextSeq 500 instrument according to Illumina and 10X Genomics guidelines with 1.4–1.8 pM input and 1% PhiX control library spike-in (Illumina, USA). Sequencing data were processed using 10X Genomics’ Cell Ranger pipeline to generate feature/barcode matrices from raw count data.
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bioRxiv - Developmental Biology 2023Quote: ... bead-bound complexes were resuspended in tagmentation mixture (25µL reaction containing 1 µL of enzyme in 1X buffer, Illumina) and incubated at 37°C for 25minutes ...
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bioRxiv - Developmental Biology 2023Quote: ... Sequencing libraries were prepared using 1 μg of total RNA with the TruSeq Stranded mRNA Sample Prep Kit (Illumina) and the TruSeq single-index adaptor (Illumina) ...
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bioRxiv - Pathology 2023Quote: ... Barcoded libraries from each experimental sample were combined in equimolar concentrations of 1.5 pM prior to sequencing at 75bp x 1 (single-end) read metric on a NextSeq 550 (Illumina) system.