Labshake search
Citations for Illumina :
201 - 250 of 340 citations for 8 16 Pyranthrenedione since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2020Quote: ... and subsequently another 8-12 PCR cycles with the specific primers (Illumina Nextera XT index kit); samples were cleaned with PCR purification kit (Qiagen ...
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bioRxiv - Genomics 2022Quote: ... DNA was genotyped at the Children’s Hospital of Philadelphia’s Center for Applied Genomics using the Infinium Omni2.5-8 v1.3 BeadChip (Illumina) on an iScan System instrument (Illumina).
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bioRxiv - Neuroscience 2023Quote: ... Biotinylated cDNA (750 ng) was hybridized at 48°C to MouseRef-8 v2 expression beadchips (Illumina) for 16 h before washing and analyzing according to the manufacturer’s directions ...
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bioRxiv - Cell Biology 2023Quote: ... 8 µL of 100 pM pooled libraries were loaded into lanes of a HiSeq 4000 (Illumina). At least 30M reads per library were generated ...
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bioRxiv - Microbiology 2019Quote: Tag-encoded 16S rRNA gene amplicon sequencing was performed on an Illumina NextSeq using v2 MID output 2×150 cycles chemistry (Illumina, CA, USA). DNA extraction and library building for amplicon sequencing was performed in accordance with Krych et al.[19] ...
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bioRxiv - Microbiology 2019Quote: 16S amplicon sequencing targeting the V3 and V4 variable regions of the 16S rRNA (341F: 5’-TCGTCGGCAGCGTCAGATGTGTATAAGAGACAGCCTACGGGNGGCWGCAG-3’ and 805R: 5’- GTCTCGTGGGCTCGGAGATGTGTATAAGAGACAGGACTACHVGGGTATCTAATC C-3’) was performed on the Illumina MiSeq platform (Illumina, California, USA) according to manufacturer’s guidelines and generated paired-end reads of 300bp in each direction ...
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bioRxiv - Microbiology 2022Quote: ... Bacterial 16S rRNA V4 region was amplified during library preparation via Illumina’s Nextera XT Index Kit v2 (Illumina, Inc.; San Diego, CA) (primers ...
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bioRxiv - Microbiology 2020Quote: ... Samples were normalized to 1.67 × 105 16S rRNA gene copies/μL prior to barcoding and Illumina MiSeq sequencing (reagent kit v3; Illumina, San Diego, CA) at the University of Minnesota Genomics Center (Minneapolis ...
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bioRxiv - Microbiology 2022Quote: ... The V4 region of the 16S rRNA gene was amplified from 1 or 7μl DNA and sequenced with a MiSeq (Illumina, San Diego, CA) using the 500 cycle MiSeq Reagent Kit ...
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bioRxiv - Microbiology 2022Quote: ... The library preparation and sequencing was performed by the Host Microbe Systems Biology Core at UC Davis using primer pair 341F and 806R in 300 bp paired-end run for the V3-V4 hypervariable regions of the 16S rRNA on an Illumina MiSeq (Illumina, San Diego). The 16S rRNA Raw FASTQ sequence files were deposited and processed in QIITA (36 ...
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bioRxiv - Genomics 2019Quote: Amplicons of the 16S rRNA gene were sequenced through Illumina MiSeq 2×250 paired-end technology (Illumina®, San Diego, California, USA) at the Unidad de Secuenciación Masiva of the UNAM Biotechnology Institute (Cuernavaca ...
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bioRxiv - Microbiology 2019Quote: Tag-encoded 16S rRNA gene amplicon sequencing was performed on a Illumina NextSeq using v2 MID output 2×150 cycles chemistry (Illumina, CA, USA). DNA extraction and library building for amplicon sequencing was performed in accordance to Krych et al ...
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bioRxiv - Microbiology 2022Quote: The V3–V4 region of the 16S rRNA amplicons was subjected to high-throughput sequencing using a MiSeq system (Illumina, United States) and MiSeq Reagent Kit v2 (500 cycles ...
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Genomic local adaptation of a generalist plant species to pollinator communities and abiotic factorsbioRxiv - Plant Biology 2022Quote: ... with 16 threads of freebayes running in parallel across regions of 100kb in the de novo polished genome assembly (PacBio, Illumina and Bionano). Single nucleotide polymorphisms (SNPs ...
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bioRxiv - Pathology 2023Quote: Genomic DNA from snap-frozen fecal samples and the amplification of hypervariable V3-V4 regions of their 16S rRNA gene were prepared as described previously.[34] Libraries preparation and sequencing (Illumina Miseq cartridge) were performed by the Genotoul facility (Get-Biopuces ...
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bioRxiv - Microbiology 2023Quote: ... The microbial community of each sample was analyzed by 16S rRNA gene amplicon sequencing of the V4-V5 hypervariable region (Illumina MiSeq platform) (Illumina Inc. ...
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bioRxiv - Microbiology 2024Quote: ... The RNA was treated with the QIAseq FastSelect 5S/16S/23S rRNA removal kit prior to library prep with the TruSeq Stranded Total RNA Library Prep Kit (Illumina, CA, USA). The Illumina HISeq 4000 was used for sequencing at 50bp single-end reads.
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bioRxiv - Microbiology 2024Quote: ... The 16S rRNA amplicons and ITS amplicons were pooled separately and sequenced using the Illumina MiSeq instrument (Illumina, San Diego, CA, USA). The raw sequences were quality filtered using the Quantitative Insights into Microbial Ecology pipeline (QIIME v1.9) ...
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bioRxiv - Microbiology 2020Quote: ... was performed with ~8 million reads / sample in single-end mode on the NextSeq 500 platform (Illumina) with the High Output Kit v2.5 (75 Cycles).
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bioRxiv - Microbiology 2021Quote: ... pooled and sequenced using two lanes of HiSeqX (Illumina; 150bp paired-end; read length 28*8*91). Two biological replicates of each L ...
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bioRxiv - Genetics 2022Quote: ... Individual sequencing barcodes were added to each sample by amplifying the 20 μL elution in a 50 μL Q5 NEBNext reaction with 0.5 uM of TruSeq_Universal_Adapter primer and a reverse primer containing a unique 8 bp index (Illumina_Multiplex) for sample demultiplexing post-sequencing ...
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bioRxiv - Cancer Biology 2019Quote: ... gene expression analysis was performed using Illumina’s Mouse Ref 8 Beadchip v1 (Illumina Inc, San Diego, CA). These datasets have been deposited in GEO as accession entry GSE22520 and described in Supplemental Table 1c ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... Samples with a RIN >8 was outsourced to Novogene for library construction and sequencing (Illumina Platform (PE150)) with 20 M raw reads/sample ...
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bioRxiv - Microbiology 2021Quote: ... Each pool of V4-V5 16S rRNA amplicons was sequenced (2×300nt paired end) on one lane of a MiSeq V2 sequencer (Illumina, San Diego, CA) at the Carver Biotechnology Center of the University of Illinois ...
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bioRxiv - Microbiology 2022Quote: The V4 region of the 16S rRNA gene was amplified from extracted fecal DNA and sequenced on a MiSeq (Illumina, San Diego, CA) using 2 x 250 V2 chemistry following the MiSeq Wet Lab SOP [(22) ...
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bioRxiv - Molecular Biology 2020Quote: ... Sequencing of the 16S rRNA gene amplicons was conducted on the Illumina Mi-Seq DNA sequencing platform (Illumina Inc., San Diego, CA, U.S.A.). Amplicon sequence data was analyzed using an internal pipeline known as the Amplicon Sequencing Analysis Pipeline (Zhang et al. ...
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bioRxiv - Microbiology 2022Quote: ... a sequencing library of the V3-V4 region of the 16S rRNA gene was constructed following the manufacturer’s instructions (Part # 15044223Rev.B; Illumina Inc., San Diego, CA, USA) with improvement as previously described [20] ...
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bioRxiv - Microbiology 2022Quote: ... A sequencing library of the V3-V4 region of the 16S rRNA gene was constructed following the manufacturer’s instructions (Part # 15044223Rev.B; Illumina Inc., San Diego, CA, USA) with improvement as previously described [20] ...
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bioRxiv - Microbiology 2022Quote: ... Microbial composition of intestinal biopsies was determined by Illumina MiSeq paired-end sequencing of the V3-V4 hypervariable region of the 16S rRNA gene (MiSeq Benchtop Sequencer, Illumina Inc., San Diego, USA). Amplification of bacterial DNA was performed by PCR using modified 341F and 806R primers with a six-nucleotide barcode on the 806R primer for multiplexing [101 ...
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bioRxiv - Microbiology 2020Quote: ... the V3–V4 region of bacterial 16S rRNA genes was sequenced using primers 341F-806R [25] with MiSeq technology (Illumina, San Diego, CA). Internal company standards worked as expected (Supplementary Methods) ...
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bioRxiv - Microbiology 2022Quote: ... for amplicon sequencing of the V3-4 hypervariable region of the 16S rRNA gene (position 341-806) on a MiSeq desktop sequencing platform (Illumina, San Diego, CA) operated under paired-end mode ...
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bioRxiv - Microbiology 2022Quote: ... China) for PCR amplification of the V4 region of the 16S rRNA gene and sequencing using an Illumina NovaSeq (Illumina, San Diego, CA) in two sequencing runs ...
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bioRxiv - Microbiology 2022Quote: ... for amplicon sequencing of the V3-4 hypervariable region of the 16S rRNA gene (position 341-806) on a MiSeq desktop sequencing platform (Illumina, San Diego, CA) operated under paired-end mode ...
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bioRxiv - Genomics 2023Quote: ... multiplexed at even concentration and sequenced on 600 bp (2 × 300bp) pair-end using Illumina MiSeq platform (Illumina, Inc., USA) (EMP 16S Illumina Amplicon Protocol, n.d.).
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bioRxiv - Microbiology 2023Quote: The V1 and V2 regions of the 16S rRNA gene were amplified using universal bacterial primers 27F and 355R74 with CS1 and CS2 adapters (Illumina, San Diego, CA). PCR was conducted with the following reaction composition ...
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bioRxiv - Microbiology 2024Quote: ... The V4 – V5 region of the 16S rRNA gene was amplified with the 515F/926R primer pair (45,46) and sequenced on the MiSeq platform (Illumina, Inc., San Diego, CA).
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bioRxiv - Genetics 2021Quote: ... 1 µg of high quality total RNA sample (RIN >8) was processed using TruSeq Stranded mRNA kit (Illumina) according to manufacturer instructions ...
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bioRxiv - Molecular Biology 2022Quote: ... Library preparation of high-quality RNA (RIN >8) was performed using the TruSeq RNA sample preparation kit (Illumina). Raw sequencing data was aligned to human genome B38 ...
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bioRxiv - Developmental Biology 2019Quote: ... with RIN values > 8 were subjected to Automated TruSeq stranded total RNA with RiboZero Gold library preparation (Illumina). Single-end 50 bp reads were generated with HiSeq2500 ...
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bioRxiv - Genomics 2020Quote: ... Genome-wide genotyping of 200 ng genomic DNA from the remaining 207 patients was carried out using Infinium HumanOmni2.5-8 v1.2 BeadChip microarrays (Illumina). After completion of the assay ...
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bioRxiv - Genomics 2020Quote: ... Individual libraries for each of the analyzed bovines (N=16) were processed using the TruSeq Stranded mRNA Preparation kit (Illumina, San Diego, CA, USA) according to manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: ... Samples were sequenced following amplification of the V4 hypervariable region of the 16S rRNA gene with modified F515/R806 primers [21] on a MiSeq platform (Illumina, San Diego, CA, USA) in 2016 (Batch 1 ...
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bioRxiv - Microbiology 2020Quote: ... of the 16S rRNA gene and the internal transcribed spacer (ITS) 1 rDNA variable region on the Illumina MiSeq platform (Illumina, San Diego, CA, USA), as detailed previously (Phoma et al. ...
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bioRxiv - Cancer Biology 2020Quote: ... The amplification and sequencing of 16S rRNA gene V3V4 region were done commercially by Omega Bioservices (Georgia, USA) on a MiSeq platform (Illumina, Inc, San Diego, CA) using the 2×300 bp paired-end protocol ...
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bioRxiv - Molecular Biology 2019Quote: ... and 16 PCR positive controls) was sequenced on an Illumina MiSeq® using a MiSeq Reagent Kit v3 (600-cycle) (Illumina, Inc, CA, USA). Raw sequence reads were demultiplexed using a custom Python script ...
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bioRxiv - Plant Biology 2020Quote: ... for amplification and sequencing of the V4-V5 (primers 518f and 926r) region of the 16S rRNA gene (40) on an Illumina MiSeq (Illumina, San Diego, CA, USA).
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bioRxiv - Microbiology 2021Quote: ... High-throughput amplicon sequencing covering the V4-V5 region of the 16S rRNA gene was performed on an Illumina Miseq 2 × 300 paired-end platform (Illumina, San Diego, CA USA) using the universal primers 515F (GTGYCAGCMGCCGCGGTAA ...
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bioRxiv - Plant Biology 2020Quote: ... on Thermo Fisher Scientific’s Quantstudio 5 before multiplex pooling (13-16 samples per lane in equal representation) and sequencing on the NextSeq500 platform (Illumina, San Diego, California, USA) paired-end 2×150 bp High Output.
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bioRxiv - Microbiology 2020Quote: The bacterial compositions of distal ileal mucosa and gut luminal content were determined by 16S rRNA gene (V3-region) amplicon sequencing on a NextSeq using PE150 (Illumina, San Diego, CA, USA). Total DNA was extracted using Bead-Beat Micro AX Gravity Kit (A&A Biotechnology ...
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bioRxiv - Genomics 2021Quote: DNA and RNA extracted from filtered water samples were diluted to a common concentration (1ng/ul) and sent for 16S rRNA amplicon sequencing on an Illumina MiSeq (Illumina, San Diego, CA, USA) according to Earth Microbiome protocols ...