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Citations for Illumina :
401 - 450 of 1129 citations for 1 Benzyl 3 Tert Butyldimethylsilyl Oxy Methyl Piperidin 4 One since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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THE OLFACTORY RECEPTOR Olfr78 REGULATES DIFFERENTIATION OF ENTEROCHROMAFFIN CELLS IN THE MOUSE COLONbioRxiv - Cell Biology 2023Quote: ... before processing through the Chromium Next GEM Single Cell 3’ Reagent Kits V3.1 (10X Genomics) and sequenced on a Novaseq 6000 (Illumina).
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bioRxiv - Cancer Biology 2023Quote: ... was constructed using the QuantSeq 3’ mRNA-seq FWD kit (Lexogen) and the UMI Second Strand Synthesis Module (Illumina) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2023Quote: ... Gel-in-beads and libraries were generated using Chromium Next GEM Single Cell 3’ Reagent Kits v3.1 (10x Genomics) and sequenced using NovaSeq (Illumina). Cell Ranger 7.0.1 ...
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bioRxiv - Developmental Biology 2024Quote: ... Cells were submitted for 10x library preparation with v3.0 chemistry for 3’ single-cell sequencing on a NovaSeq 6000 (Illumina) at the Cancer Research UK Cambridge Institute.
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bioRxiv - Cancer Biology 2024Quote: ... Single-cell libraries were generated using 10X Genomics Chromium Single-cell 3’ Library RNA-Seq Assays protocols targeting 8,000 cells from each fraction were sequenced on the NovaSeq sequencer (Illumina). The scRNA-seq data were analyzed with the Partek Flow software (Partek Inc) ...
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bioRxiv - Developmental Biology 2024Quote: ... Cells were submitted for 10x library preparation with v3.0 chemistry for 3’ single-cell sequencing on a NovaSeq 6000 (Illumina) at the Cancer Research UK Cambridge Institute.
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bioRxiv - Genetics 2024Quote: ... cDNA libraries were constructed using the QuantSeq 3′ mRNA-Seq Library Prep Kit FWD (Illumina, Lexogen GmbH, Vienna, Austria) following manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2024Quote: ... cDNA sequencing was performed by the High Throughput Sequencing Core (CHOP) using a standard 3’ assay with the NovaSeq 6000 platform and S1 Reagent Kit v1.5 (Illumina).
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bioRxiv - Molecular Biology 2024Quote: ... ScRNA-seq libraries were prepared using the 10x Genomics Chromium Next GEM Single Cell 3′ Library Construction Kit V3.1 (CG000204. Generated libraries were sequenced on an Illumina HiSeq4000 for 150bp paired end reads ...
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bioRxiv - Cell Biology 2024Quote: Generation of 3’ mRNA sequencing libraries was done according to manufacturer’s recommendations (Lexogen, 015-QuantSeq 3′ mRNA-Seq Library Prep Kit FWD for Illumina, 081-UMI Second Strand Synthesis Module for QuantSeq FWD ...
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bioRxiv - Cell Biology 2024Quote: ... with the Chromium Single Cell 3′ v.3.1 kit(10x Genomics, no. PN- 1000121) and sequenced on the NovaSeq 6000 platform (Illumina) to obtain around 50,000 reads per cell.
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bioRxiv - Cancer Biology 2024Quote: Gene analysis was performed thanks to the GenoBird platform by 3’RNA-sequencing profiling using a NovaSeq 6000 (Illumina). The quality of raw sequence reads was assessed by FastQC ...
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bioRxiv - Cell Biology 2024Quote: ... Libraries were prepared using the 10x genomics chromium 3’ gene expression solution v3.1 and sequenced on a NovaSeq6000 (Illumina). Read counts were analysed using Seurat (v5) ...
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bioRxiv - Cell Biology 2024Quote: ... After 2-3 passages in complete DMEM with 10% FBS gDNA was extracted to confirm the genotype by Illumina amplicon sequencing and live cell microscopy was performed.
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bioRxiv - Genomics 2020Quote: ... denatured and diluted to 4 pM before loading onto the MiSeq flow cell (Illumina Inc., United States). According to Illumina protocol ...
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bioRxiv - Molecular Biology 2020Quote: ... Nuclei were then pelleted (1,000xg, 10min, 4°C) and transposed in 2.5µl Tn5 (Nextera Tn5 transposase, Illumina), 12.5µl TD reaction buffer and 10.5µl water for 40min at 37°C with rotation ...
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bioRxiv - Genomics 2020Quote: ... The library was sequenced on 4 lanes of an Illumina HiSeq 1500 (Illumina Inc.; San Diego, CA), generating 450,132,548 reads which were subsequently trimmed to remove adapters and filtered for length and quality using FASTX-Toolkit v0.0.14 (available from http://hannonlab.cshl.edu/fastx_toolkit/) ...
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bioRxiv - Genomics 2021Quote: ... Libraries were diluted to 4 nM and sequenced on a NextSeq 500 Sequencing System (Illumina, California, USA) using NextSeq 500/550 High Output Kit v2 (150 cycles ...
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bioRxiv - Genomics 2021Quote: ... The library pool was sequenced on all 4 lanes of an NovaSeq 6000 S4 flow cell (Illumina) for 150 bp paired end reads ...
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bioRxiv - Molecular Biology 2020Quote: ... Small RNA-seq libraries for 2–4 biological samples were sequenced together using a NextSeq 500 (Illumina) to obtain 75 nt ...
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bioRxiv - Genomics 2021Quote: ... Nuclear isolation of 5×10^4 cells was followed by treatment with Nextera Tn5 enzyme (Illumina, 20034198) for 45 minutes at 37°C ...
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bioRxiv - Immunology 2023Quote: ... We observed less than 4% barcode swapping between the two barcodes (more than 96% of the Illumina sequencing reads contained correct endodomain and barcode pairs) ...
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bioRxiv - Cancer Biology 2021Quote: ... libraries were prepared using a Chromium Single Cell 3’ Library & Gel Bead kit v2 (10x Genomics, PN-120237) and sequenced using a NextSeq 500 (Illumina). The mean number of reads per cell was approximately 25,000 and the median number of genes detected per cell was approximately 2,000.
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bioRxiv - Cell Biology 2020Quote: ... Indexed sequencing libraries were constructed using the Chromium Single-Cell 3’ library kit (10X Genomics) and sequenced on NovaSeq 6000 (Illumina) with the following parameters ...
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bioRxiv - Genetics 2021Quote: ... 3) SpliceAI (SAI)11 scores were retrieved using a script adapted from the SAI github repository (https://github.com/Illumina/SpliceAI) which allows spliceAI to score custom sequences ...
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bioRxiv - Microbiology 2022Quote: ... Total RNAs samples (3 μg) were depleted from ribosomal RNA using the bacteria Ribo-Zero® rRNA Removal kit (Illumina) according to the manufacturer’s protocol ...
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bioRxiv - Genomics 2020Quote: ... Multiple mate-paired libraries (3, 8, 12 and 16 kb) were also constructed using the Nextera Mate-Paired Library Construction kit (Illumina). Libraries were sequenced on the Illumina HiSeq 2500 sequencer using the Illumina TruSeq PE Cluster kit v3 and TruSeq SBS kit v3 (101 ...
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bioRxiv - Immunology 2020Quote: ... cDNA was harvested and diluted to 0.1–0.3 ng/μl and libraries were prepared in 96-well plates using a Nextera XT DNA Sample Preparation kit (Illumina) according to the protocol supplied by Fluidigm ...
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bioRxiv - Neuroscience 2020Quote: Tagmentation was performed per pool by adding 3 μL double-stranded cDNA sample to 5.5 μL Nextera TD buffer (Illumina, 15027866), 2.5 μL NF H2O and 1.0 μL TDE1 Enzyme (Illumina ...
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bioRxiv - Systems Biology 2020Quote: ... 8 pools of 24 randomized libraries were sequenced in 3 lanes at 100 bp single-end on the HiSeq 2500 (Illumina) using TruSeq SBS Kit v4 reagents (Illumina).
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bioRxiv - Neuroscience 2020Quote: ... The cDNA was then fragmented and amplified for 3’ prime end sequencing with the Nextera XT DNA sample prep kit (Illumina). The libraries were purified ...
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bioRxiv - Genomics 2020Quote: ... sequencing libraries were prepared with the QuantSeq 3’ mRNA-Seq Library Prep Kit FWD (Lexogen) and sequenced on a HiSeq 4000 Sequencing System (Illumina) using the HiSeq3000/4000 SR Cluster and SBS Kit (Illumina).
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bioRxiv - Microbiology 2021Quote: ... the sgRNA coding sequences integrated in the genomic DNA from the initial and 3-times selected populations were amplified by touch-down PCR and sequenced by Illumina deep sequencing ...
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bioRxiv - Molecular Biology 2021Quote: ... 150 ng of RNA was used for library preparation with the Lexogen QuantSeq 3’ mRNA-Seq Library Prep Kit (FWD) from Illumina. Quality control of the sequencing libraries was performed with both Qubit™ (DNA HS Assay kit ...
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bioRxiv - Cancer Biology 2020Quote: The RNA-seq libraries (n=3 per experimental group) were prepared using the NEBNext Ultra II RNA library prep kit from Illumina (New England Biolabs Inc. ...
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bioRxiv - Cancer Biology 2020Quote: ... and the analysis was run using sequencing reads of 3 or higher from 454 data and 10 or greater reads from Illumina. Single cell sequencing was performed as described previously (Mann et al. ...
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bioRxiv - Neuroscience 2022Quote: ... cells were harvested and prepared with 10X Chromium Single Cell 3’ Reagents V3 and sequenced on a NovaSeq 6000 (Illumina) using a S2 flow cell at 2 × 100bp ...
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bioRxiv - Cell Biology 2022Quote: ... RNA library was constructed with 10x-Genomics Single Cell 3’ RNA sequencing kit v3 as previously described(18) sequenced using a NextSeq500 (Illumina). Cell clusters were characterized using an automated annotation tool(19 ...
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Patient-associated mutations in Drosophila Alk perturb neuronal differentiation and promote survivalbioRxiv - Cancer Biology 2022Quote: ... and libraries prepared using the normal workflow for Single Cell 3’ v3 libraries (10X Genomics) and sequenced on the NextSeq 500 platform (Illumina). Initial preprocessing of scRNA-seq datasets began with demultiplexing ...
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bioRxiv - Cancer Biology 2022Quote: ... using primers DCF01 5’-CTTGTGGAAAGGACGAAACACCG-3’ and DCR03 5’-CCTAGGAACAGCGGTTTAAAAAAGC-3’ and subjected to single-end 75 bp (SE75) high-throughput sequencing using a NextSeq550 (Illumina).
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bioRxiv - Developmental Biology 2021Quote: ... and cDNA synthesis and library construction were performed as per the manufacturer’s protocol for the Chromium Single Cell 3′ v2 protocol (10x Genomics; PN-120233) and sequenced on an Hiseq4000 (Illumina). 10x CellRanger (version 3.0.2 ...
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bioRxiv - Neuroscience 2022Quote: ... scRNA-seq libraries were prepared with the Chromium Single Cell 3′ Library & Gel Bead Kit v.3 (10x Genomics, PN-1000075).Ready 10X libraries were sequenced paired end (R1:28, R2: 89 cycles) on NovaSeq (Illumina).
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bioRxiv - Cancer Biology 2022Quote: ... performed the library preparation for WGS of parental and resistant cells of cell lines #3 and #9 as well as a control tail samples corresponding to line #3 with the TruSeq DNA PCR-free Kit (Illumina) and the 150 bp paired-end sequencing on a HiSeq X (Illumina).
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bioRxiv - Cancer Biology 2020Quote: ... 500 ng RNA was prepared with a commercially available kit according to the manufacturer’s instruction (QuantSeq 3′ mRNA-Seq Library Prep Kit FWD for Illumina and PCR Add-on Kit for Illumina, Lexogen). Sequencing was performed on an Illumina HiSeq2500 in 50bp single-read mode.
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bioRxiv - Cancer Biology 2020Quote: ... 500 ng alkylated RNA was used and prepared with a commercially available kit (QuantSeq 3′ mRNA-Seq Library Prep Kit FWD for Illumina and PCR Add-on Kit for Illumina ...
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bioRxiv - Developmental Biology 2020Quote: ... and two different mate-pair libraries (3 kb and 5 kb) were prepared with a Nextera Mate Pair Library Prep Kit (Illumina). Sequencing libraries were run on the Illumina Hiseq 2500 sequencer with a read length of 150 bp ...
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bioRxiv - Developmental Biology 2020Quote: ... Libraries were constructed with version 2 (stage 16a) or version 3 (stage15 a/b) chemistry and sequenced on a single HiSeqX (Illumina) lane to generate 400-450 million paired-end 150 bp reads (Supplementary Table 4).
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bioRxiv - Cancer Biology 2020Quote: ... libraries for mRNA sequencing were prepared using the QuantSeq 3’ mRNA FWD kit (Lexogen) prior to sequencing on the Nextseq500 (High output, SR75, Illumina). On average 7.0 ± 1.9M high-quality reads were generated per samples ...
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bioRxiv - Immunology 2020Quote: ... Single cell RNA libraries were prepared according to the 10x Genomics Chromium Single Cell 3’ Reagent Kits v2 User Guide and sequenced on a HiSeq4000 (Illumina).
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bioRxiv - Genomics 2021Quote: ... cells and CD14+ monocytes were generated using the SureCell WTA 3′ Library Prep Kit for the ddSEQ System (20014280, Illumina) with the following modifications ...