Labshake search
Citations for Illumina :
3701 - 3750 of 3798 citations for Probable U3 small nucleolar RNA associated protein 11 UTP11 Antibody FITC since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genomics 2024Quote: Illumina short-read sequencing libraries were prepared with 1 μg of high-quality RNA and the TruSeq Stranded mRNA Library Prep Kit (Illumina, San Diego, CA) according to manufacturer’s protocol ...
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bioRxiv - Genomics 2024Quote: ... then reverse transcribed to cDNA and prepared for next-generation sequencing according to the manufacturer’s protocol (Illumina TruSeq RNA Sample Preparation v2 Guide). The cDNA libraries were validated on the Agilent 2100 Bioanalyzer using DNA-1000 chip ...
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bioRxiv - Pathology 2024Quote: ... 200 ng RNA was used to prepare sequencing libraries using the TruSeq Stranded Total RNA with RiboZero following the low sample protocol as per manufacturer’s recommendations (Illumina, San Diego, CA, USA), as previously described43 ...
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bioRxiv - Neuroscience 2024Quote: ... RNA samples were sent to Admera Health for library construction (SMARTseq V4 + Nextera XT) and sequencing (Illumina 2x 150bp reads, 40M read depth).
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bioRxiv - Neuroscience 2024Quote: ... cDNA was synthesized from each individual sample using random hexamer primers and libraries were prepared by TruSeq RNA sample preparation kit (Illumina, San Diego, USA). RNA-seq analysis (50 bp ...
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bioRxiv - Molecular Biology 2024Quote: 500 ng of total RNA served as the input material for library preparation using the TruSeq Stranded mRNA Library Prep kit (Illumina, cat. no. 20020595), along with the IDT for Illumina – TruSeq RNA UD Indexes v2 (Illumina ...
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bioRxiv - Microbiology 2024Quote: All bacterial strains to be sequenced were grown overnight in LB in 15 ml falcon tubes and DNA samples were obtained using the Epicentre MasterPure Complete DNA and RNA Purification Kit (Illumina, San Diego, USA). DNA libraries were prepared using the Illumina DNA Prep kit according to the manufacturer’s guidelines ...
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bioRxiv - Cell Biology 2024Quote: ... The libraries for RNA sequencing were sequenced with 2 × 150-bp paired-end reads on a NovaSeq6000 (Illumina Inc., San Diego, CA, USA). The obtained sequencing data were analyzed using RaNA-seq (61) ...
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bioRxiv - Genomics 2024Quote: ... mRNA enrichment and preparation of barcoded cDNA libraries were done using the TruSeq RNA Sample Prep Kit (Illumina, Inc., San Diego, CA, USA). F ...
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bioRxiv - Biochemistry 2024Quote: ... Subsequent next-generation RNA sequencing was outsourced and conducted at Kyushu Pro Search LLP (Fukuoka, Japan) using Nextseq 500 (Illumina, San Diego, CA). Lead count data were analyzed using integrated Differential Expression & Pathway Analysis 2.01 (iDEP2.01 ...
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bioRxiv - Genomics 2024Quote: RNA sequencing libraries were produced using the Illumina Truseq Stranded mRNA process and sequenced on an Illumina HiSeq 2500 (Illumina, San Diego, CA) by the Otago Genomics Facility ...
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bioRxiv - Cell Biology 2024Quote: ... and samples with an RNA Integrity Number (RIN) greater than 8.0 were used to generate RNA sequencing libraries using the Illumina® Stranded mRNA Prep (Illumina, San Diego, CA). Samples were processed following manufacturer’s instructions ...
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bioRxiv - Evolutionary Biology 2021Quote: ... Library preparation for Illumina sequencing was performed at the Transcriptome and Genome Analysis Laboratory Goettingen (TAL) using the TruSeq RNA Library Prep Kit v2 (Illumina, San Diego, CA, USA). Pooled libraries were run on an Illumina HiSeq 4000 to produce 50bp single end reads ...
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bioRxiv - Evolutionary Biology 2022Quote: Genomic DNA from all smut species was isolated using a MasterPure™ Complete DNA&RNA Purification Kit (Epicentre®, Illumina®, Madison, Wisconsin, USA) according to the manufacturer’s instructions ...
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bioRxiv - Genomics 2022Quote: RNA-Seq library preparations were carried out from 500ng to 2000ng of total RNA using the TruSeq Stranded mRNA kit (Illumina, San Diego, CA, USA), which allows mRNA strand orientation (sequence reads occur in the same orientation as antisense RNA) ...
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bioRxiv - Genomics 2020Quote: Fragmented cDNA Sequencing libraries were generated from 100ng of Total RNA using NEBNext Ultra II with polyA isolation module (Illumina, San Diego, California, USA) according to manufacturer’s protocol ...
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bioRxiv - Genomics 2020Quote: Total RNA was extracted from Epstein–Barr-virus-transformed lymphoblastoid cell lines (LCLs) by following the Illumina TruSeq v2 RNA Sample Preparation protocol (Illumina, Inc., San Diego, CA) by the Department of Genetic Medicine and Development at the University of Geneva ...
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bioRxiv - Genomics 2020Quote: RNA libraries from all samples were prepared for shotgun transcriptomic sequencing using the TruSeq Stranded Total RNA Library kit from Illumina (San Diego, CA, USA), following manufacturer’s instructions ...
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bioRxiv - Genomics 2020Quote: ... was prepared from total RNA from a testicular tissue sample of one BSW bull homozygous for the mutant T-allele at Chr6:58373887 using the Illumina TruSeq RNA Sample Preparation Kit (Illumina, San Diego, CA, USA). The library was sequenced using an Illumina NovaSeq6000 instrument ...
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bioRxiv - Cancer Biology 2021Quote: ... 200 ng of total RNA was used to construct a TruSeq strand-specific library with the Ribo Zero protocol (Illumina, Cat. #RS-122-2203). The libraries were sequenced on a HiSeq 2000 to a minimal target of 180 million ...
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bioRxiv - Immunology 2021Quote: ... libraries were prepared using the NEXTflex®Rapid Directional RNA-Seq Kit (Bio Scientific) and samples were sequenced 75 base pair single-end on Illumina NextSeq500 (Illumina, Utrecht DNA Sequencing Facility) and processed as described above ...
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bioRxiv - Zoology 2021Quote: ... Meta-transcriptome library preparation of each pool was carried out using the TruSeq™ Stranded Total RNA Sample Preparation Kit from Illumina (San Diego, CA) after removal of host ribosomal RNA using Illumina Ribo-Zero™ rRNA Removal Kits (San Diego ...
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bioRxiv - Evolutionary Biology 2020Quote: ... Sequencing libraries were prepared using NEBNext Ultra II directional RNA library kit and paired-end sequenced (150bp) in an Illumina HiSeq3000 (Illumina Inc., San Diego, USA) instrument ...
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bioRxiv - Cancer Biology 2021Quote: ... 356-500ng of total RNA with DV200% varying from 88-93 underwent ribosomal depletion and library preparation using the TruSeq Stranded Total RNA LT Kit (Illumina catalog # RS-122-1202) according to instructions provided by the manufacturer with 8 cycles of PCR ...
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bioRxiv - Microbiology 2020Quote: ... The RNA extracts were enriched for bacteria by depleting coral host rRNA using a Ribo-Zero Gold Yeast kit (Illumina, San Diego, CA, USA) following the manufacturer’s protocols.
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bioRxiv - Cancer Biology 2020Quote: ... RNA (1 μg) was then converted into cDNA libraries according to the Illumina TruSeq Stranded mRNA Sample Preparation Guide (Illumina, San Diego, CA, USA) following manufacturer’s instructions ...
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bioRxiv - Cell Biology 2021Quote: ... with RNA integrity number higher than 7 was used for library preparation using the TruSeq Stranded mRNA Library Preparation Kit (Illumina, San Diego, CA, USA) and sequenced on a NextSEq500 (Illumina) ...
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bioRxiv - Cancer Biology 2020Quote: ... and poly-A sequencing libraries (HE2.1 and TIVE cells) were generated using the TruSeq RNA Sample Prep Kit (Illumina, catalog no. FC-122-1001).
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bioRxiv - Neuroscience 2022Quote: ... and libraries were constructed by using 600 ng of total RNA from each sample and utilizing a TruSeqV2 kit from Illumina (Illumina, San Diego, CA) following the manufacturer’s suggested protocol ...
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bioRxiv - Immunology 2022Quote: ... A total of 250ng total RNA was used for each sample as input to the TruSeq Stranded Total RNA library prep kit with unique-dual indexing (catalog #s 20020598 & 2002371; Illumina, San Diego, CA, USA). Libraries were assessed for mass using the Qubit 1X dsDNA HS Assay kit (catalog # Q33231 ...
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bioRxiv - Microbiology 2022Quote: ... sequencing libraries were prepared using Stranded Total RNA Prep Ligation with Ribo-Zero Plus kit per manufacturer’s protocol (Illumina Inc., San Diego, CA, USA) and sequenced on an Illumina MiSeq at 2 x 150 base pair reads ...
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bioRxiv - Neuroscience 2022Quote: ... the mRNA from each brain sample was pooled for RNA-Seq library construction using TruSeq Stranded mRNA LT Sample Prep Kit (Illumina, San Diego, CA, USA). The mRNA library was subjected to paired-end transcriptome sequencing (Illumina platform) ...
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bioRxiv - Cancer Biology 2020Quote: ... 534-850ng of total RNA with DV200% varying from 38-74% was used for ribosomal depletion and library preparation using the TruSeq Stranded Total RNA LT Kit (Illumina catalog # RS-122-1202) according to manufacturer’s instructions with 8 cycles of PCR ...
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bioRxiv - Molecular Biology 2020Quote: ... Total RNA was extracted from the seedlings and libraries were constructed from three independent biological replicates following polyA selection using instructions for TruSeq RNA library preparation (Illumina protocol 15026495 Rev. B). Libraries were sequenced on the Illumina HiSeq2500 platform generating 100 bp paired-end reads ...
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bioRxiv - Evolutionary Biology 2020Quote: ... we used 2 μg total RNA for library preparation following the protocol in the TruSeq Stranded mRNA Library Prep Kit (Illumina, San Diego, CA, USA). The samples were sequenced by single-end reads (75 bp ...
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bioRxiv - Genetics 2021Quote: ... The thirty-seven sequencing libraries were created by reverse-transcription from 2 µg of RNA from each sample using the TruSeq Stranded mRNA Library Prep kit (Illumina, San Diego, CA, USA). Index adapters were added to identify sequences for each sample in the final data ...
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bioRxiv - Genomics 2021Quote: ... Pacific Biosciences Iso-Seq and Illumina RNA-Seq transcriptome libraries contributing to annotation, and three-tissue (bud, catkin, and young leaf) whole-genome bisulfite libraries (Illumina SE, ≈18x – 19x coverage) for the DNA methylomes ...
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bioRxiv - Genomics 2020Quote: ... RNA libraries from all samples were then prepared for shotgun transcriptomic sequencing using the TruSeq Stranded Total RNA Library kit from Illumina (San Diego, CA, USA), following manufacturer’s instructions ...
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bioRxiv - Genomics 2021Quote: ... For long read sequencing (Oxford Nanopore Technologies) total RNA was extracted as described above and depleted using the Ribo-Zero rRNA Removal Kit Plant Leaves (Illumina, San Diego, CA, USA). RNA was then purified and concentrated on a RNA Clean Concentrator™-5 column (Zymo Research ...
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bioRxiv - Developmental Biology 2021Quote: ... and sequencing libraries of poly(A)+ RNAs were then generated using the VAHTSTM mRNA-seq v2 Library Prep Kit from Illumina (Vazyme Biotech, NR601-01) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2020Quote: RNA concentration and quality was assessed with Ribogreen and QC length profiling by the facility before library generation (TruSeq® Illumina mRNA (Poly(A) enrichment ...
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bioRxiv - Immunology 2020Quote: ... was adjusted to 1 μg and used for poly (A) mRNA enrichment and stranded specific RNA-Seq library preparation with TruSeq Stranded mRNA kit (Illumina, San Diego, CA, USA). Quality control of prepared libraries were performed using Agilent Bioanalyzer DNA 1000 kit and Qubit DNA HS fluorescence kit ...
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bioRxiv - Immunology 2021Quote: ... Library preparation was done using a NEBNext Ultra II Directional RNA Library Prep Kit for Illumina with poly-A selection (Illumina, San Diego, CA, USA) following the manufacturer’s instructions ...
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bioRxiv - Evolutionary Biology 2022Quote: ... was used for cDNA synthesis and purification using a low-throughput protocol with a TruSeq Stranded RNA LT Kit (Illumina, San Diego, CA, USA). A half-scale reaction of the standard protocol was used for library preparation ...
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bioRxiv - Plant Biology 2022Quote: ... We prepare 12 cDNA libraries (3 individuals □ 2 sampling times (dawn and dusk) □ 2 localities) using the TruSeq RNA-seq library prep kit from Illumina (Illumina, Inc., CA, USA) according to manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2022Quote: ... Libraries were prepared from purified RNA (∼1 μg) using Illumina TruSeq standard mRNA library preparation kit as per manufacturer protocol (Illumina Inc., San Diego, CA). Briefly ...
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bioRxiv - Microbiology 2022Quote: ... All samples showed a high RNA quality (RNQ>8) and were sequenced using an Illumina® HiSeq 3000 sequencer (Illumina, San Diego, CA, USA). Reads were mapped to the Sscrofa11.1 assembly of the Swine Genome Sequencing Consortium (SGSC ...
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bioRxiv - Neuroscience 2023Quote: ... for 16p11.2 del/+ mice were prepared using the Illumina TruSeq Stranded Total RNA with Ribo-Zero gold sample preparation kit (Illumina, Inc., San Diego, CA). Pooled libraries were sequenced on Illumina NovaSeq 6000 sequencers with 100-bp Paired-End chemistry (Illumina) ...
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bioRxiv - Microbiology 2022Quote: ... the 16S and ITS amplicon PCRs were subjected to amplification with IDT for Illumina Nextera DNA Unique Dsual Indexes (Set A, now called IDT for Illumina DNA/RNA UD Indexes) (produced by ...
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bioRxiv - Neuroscience 2024Quote: Libraries of BMP2-stimulated naïve cortical cultures were prepared from 200 ng total RNA by using the TruSeq Stranded mRNA Library Kit (Cat# 20020595, Illumina, San Diego, CA, USA) and the TruSeq RNA UD Indexes (Cat# 20022371 ...