Labshake search
Citations for Illumina :
3501 - 3550 of 3798 citations for Probable U3 small nucleolar RNA associated protein 11 UTP11 Antibody FITC since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2022Quote: ... first and second strand cDNA synthesis were performed according to the TruSeq RNA Sample Preparation v2 Kit (Illumina RS-122-2001) using Superscript III for reverse transcription instead of Superscript II (incubation time ...
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bioRxiv - Molecular Biology 2022Quote: ... PolyA + fraction (1 ng for each sample) was used for whole-transcriptome library preparation using ScriptSeq v2 RNA-Seq kit (Illumina, USA). Libraries amplified in 14 PCR cycles were purified using Agencourt AMPure XP beads (Beckman-Coulter ...
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bioRxiv - Developmental Biology 2022Quote: ... The libraries prepared from total RNA or RPF for both conditions were pooled and sequenced on a NextSeq® 500 System (Illumina).
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bioRxiv - Developmental Biology 2022Quote: ... Libraries were generated at the Huntsman Cancer Institute Genomic Core facility using TruSeq Stranded Total RNA Library Prep with Ribo-Zero Gold kits (Illumina 20020598) and sequenced using the Illumina NovaSeq 6000 for 100M 2×50bp reads ...
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bioRxiv - Evolutionary Biology 2022Quote: ... RNA-seq analysis was performed by single-end sequencing (66 bp) using a HiSeq 2500 instrument (Illumina, San Diego, CA, USA). Three replicates (i.e. ...
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bioRxiv - Microbiology 2022Quote: ... night RNAseq libraries were produced according to the manufacturer’s protocol (NEBNext UltraII Directional RNA Library Prep Kit, Illumina, NEB, MA, USA) using aroung 1 000 ng of total RNA ...
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bioRxiv - Microbiology 2022Quote: DNAse-treated RNA extracts were processed for library preparation using the Truseq Stranded mRNA sample preparation kit (Illumina, San Diego, California) according to the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2022Quote: ... The RNA-seq libraries were constructed with the Illumina Whole Transcriptome Analysis Kit as the standard protocol described (Illumina, HiSeq system), and were sequenced on the HiSeq 2000 platform ...
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bioRxiv - Plant Biology 2022Quote: ... and was further processed after quality assessment and enrichment for pair-end cDNA library construction using the TruSeq RNA Library Preparation v2 Kit (Illumina, USA) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2023Quote: Non-directional Poly-A library preparations were performed according to the manufacturer’s recommendations (Next® Ultra RNA Library Prep Kit for Illumina®). Briefly ...
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bioRxiv - Cancer Biology 2023Quote: ... pre-treatment and post-treatment/acquired resistant biopsies were obtained from patients receiving various immune checkpoint inhibitor treatments (PD-L1, PD-1, CTLA-4 targeted therapies) for RNA-seq analysis (Illumina HiSeq2500) from formalin fix paraffin embedded samples ...
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bioRxiv - Cell Biology 2022Quote: For the RNA sequencing of samples from FACS sorted mECs incubated under angiogenic conditions for 16h the following protocol was used: The TruSeq stranded RNA kit (Illumina Inc.) was used according to the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2023Quote: ... the first strand cDNA was synthesized using TruSeq stranded total RNA kit and TruSeq stranded mRNA kit (Illumina, San Diego, USA), followed by the second strand cDNA synthesis using dUTP ...
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bioRxiv - Genomics 2022Quote: ... 500 ng of purified RNA with RNA integrity number (RIN) ≥8 was subsequently used for library preparation with the TruSeq Stranded mRNA library Prep (Illumina, #20020594) and sequenced on the Illumina NextSeq500 system using a paired-end 2×75 bp protocol ...
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bioRxiv - Genomics 2022Quote: ... The tagmented fragments were amplified to add index adaptor sequences using single-use Illumina® DNA/RNA UD Indexes (Cat No: 20027213, Illumina). After Clean-up ...
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bioRxiv - Evolutionary Biology 2023Quote: ... 150-bp paired-end RNA-seq libraries were prepared with the TruSeq Stranded mRNA Kit (Illumina, Inc., San Diego, CA, USA) and sequenced on a NovaSeq 6000 instrument to a depth of 100 M reads per library by Psomagen ...
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bioRxiv - Developmental Biology 2023Quote: ... Total RNA of 1μg was used to generate libraries using of Illumina stranded total RNA prep with Ribo-Zero Plus library preparation kit (Illumina, 20040529). The Qubit dsDNA HS (High Sensitivity ...
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bioRxiv - Cancer Biology 2023Quote: ... Samples were additionally quantified by RiboGreen and assessed for quality control using an Agilent BioAnalyzer in the Integrated Genomics Core at MSKCC and 513 ng to 1.0 µg of total RNA with an RNA integrity number ranging 1.3–8.3 underwent ribosomal depletion and library preparation using the TruSeq Stranded Total RNA LT kit (Illumina, RS-122-1202) according to instructions provided by the manufacturer with eight cycles of PCR ...
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bioRxiv - Microbiology 2023Quote: We evaluated whether SEARCH-TB identified the same transcriptional changes as a conventional RNA-seq method without Mtb targeted amplification (Illumina TrueSeq) after 24-hour in vitro isoniazid (INH ...
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bioRxiv - Physiology 2024Quote: ... Stranded libraries were prepared with 100 ng of total RNA with the TruSeq Stranded mRNA Library Prep for NeoPrep Kit (Illumina, Inc.) per the manufacturer’s instructions ...
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bioRxiv - Genetics 2024Quote: Total RNA from mouse glomeruli was sequenced on a HiSeq 4000 following standard workflow using NEBNext Ultra II RNA Library Preparation kit (New Englab Biolabs, MA)(Illumina, CA) and paired-end sequencing (Genewiz ...
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bioRxiv - Developmental Biology 2024Quote: ... at 1/8th reaction volumes (except for Cas13d control and bckdk mRNA KD samples where 1/4th of the reaction volume were used) paired with IDT for Illumina DNA/RNA UD Indexes Set A (Illumina, 20027213), and purified using the Ampure XP bead-based reagent (Beckman Coulter ...
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bioRxiv - Cell Biology 2024Quote: Sequencing libraries were prepared by the University of Chicago Genomics Facility from DNase I treated RNA using Illumina RiboZero (Illumina #MRZY1306) and Illumina TruSeq library prep kits ...
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bioRxiv - Cancer Biology 2024Quote: ... Demultiplexed read sequences were aligned to the Mouse Genome (mm10) reference sequence using TopHat58 splice junction mapper with Bowtie 259 or STAR60 aligners (RNA-seq Alignment app, Illumina Basespace). Assembly was performed and differential expression were estimated using Cufflinks61 and Cuffdiff62 ...
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bioRxiv - Plant Biology 2024Quote: RNA-seq libraries were constructed by the POPS platform (IPS2) using the Illumina Stranded mRNA Prep kit (Illumina®, California, U.S.A.) according to the supplier’s instructions ...
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bioRxiv - Microbiology 2024Quote: ... The cDNA libraries were constructed using the NEBNext® Ultra II Directional RNA Library Prep Kit for Illumina® and sequenced in paired-end (2×150 bp) on a NovaSeq 6000 (Illumina). After the confirmation of multiple virus infections in H ...
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bioRxiv - Neuroscience 2024Quote: ... Half of the obtained dephosphorylated footprint RNA was processed further for rRNA depletion using Ribo-Zero Gold rRNA Removal Kit (Human/Mouse/Rat) (Illumina, MRZG12324). For each reaction ...
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bioRxiv - Plant Biology 2024Quote: ... which subsequently constructed cDNA libraries for RNA-seq according to the TruSeq stranded mRNA library preparation protocol (Illumina, San Diego, USA). Sequencing was performed on a NovaSeq 6000 S4 flow cell machine (Illumina ...
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bioRxiv - Molecular Biology 2024Quote: ... first and second strand cDNA synthesis were performed according to the TruSeq RNA Sample Preparation v2 Kit (Illumina, RS-122-2001) but using Superscript III for reverse transcription instead of Superscript II (50 °C for 50’ incubation time) ...
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bioRxiv - Immunology 2024Quote: ... The bar coded RNA libraries were pooled in equimolar ratios prior to sequencing using the Illumina NovaSeq 6000 (Illumina, CA, USA). Paired-end 102 bp reads were generated using either a NovaSeq 6000 S1 reagent kit v1.5 (200 cycles ...
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bioRxiv - Microbiology 2024Quote: ... and cDNA libraries were generated by SeqCenter using the Stranded Total RNA Prep with Ribo-Zero Plus 563 Microbiome kit (Illumina Inc). Final libraries were sequenced on an Illumina Novaseq platform with 150 bp paired-end reads ...
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bioRxiv - Microbiology 2024Quote: ... Metatranscriptomic libraries were prepared using the Illumina Stranded Total RNA library preparation kit with ribosomal depletion using Ribo-Zero Plus (Illumina Inc.). Short read Illumina NovaSeq 6000 sequencing on an SP flow cell of 2X150 bp was performed at the SNP/SEQ facility of the National Genomics Infrastructure in Uppsala ...
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bioRxiv - Synthetic Biology 2024Quote: ... Barcoded RNA-seq libraries were prepared from 1 μg of total RNA using the TruSeq Stranded mRNA Library Prep kit according to the manufacturer’s instructions (Illumina Inc., USA). RNA-seq libraries were sequenced on an Illumina NovaSeq 6000 System according to the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2024Quote: ... All samples were submitted to the TruSeq total RNA library construction for microbe with Ribo-zero kit previously to be sequenced using the NovaSeq 6000 (Illumina, CA) platform at Macrogen.
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bioRxiv - Molecular Biology 2024Quote: ... 100-500 ng of total RNA was used as input for poly(A) capture and library preparation using the Illumina Stranded mRNA kit (Illumina 20040532) per manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2024Quote: Two hundred and fifty ng of RNA were diluted to 50 uL in nuclease-free water and RNA libraries were prepared according to the TruSeq Stranded mRNA Library Prep protocol (Illumina, #20020594). HighPrep PCR beads were used for cleanup steps (MagBio Genomics ...
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bioRxiv - Cell Biology 2020Quote: ... and samples with an RNA Integrity Number (RIN) greater than 8.0 were used to generate RNA sequencing libraries using the TruSeq Stranded mRNA Sample Prep (Illumina, San Diego, CA). Samples were processed following manufacturer’s instructions ...
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bioRxiv - Neuroscience 2021Quote: ... Hippocampus RNA-seq libraries were prepared in accordance with New England Biolab protocols (NEBNext_ Ultra II RNA library Prep kit for Illumina, NEB, USA). Briefly ...
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bioRxiv - Molecular Biology 2021Quote: ... and ribosomal RNA was removed using the Ribo-Zero™ Magnetic Core Kit and Ribo-Zero™ rRNA Removal kit (Illumina, USA), according to the manufacturers’ protocols ...
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bioRxiv - Molecular Biology 2021Quote: ... Libraries were prepared using an Illumina® TruSeq® Stranded Total RNA kit with ribosomal depletion by Ribo-Zero Gold solution from Illumina® according to the manufacturer’s protocol and sequenced on an Illumina® HiSeq 4000 (2x 75 bp).
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bioRxiv - Genomics 2020Quote: ... One hundred ng of total RNA was used to construct an Illumina sequencing library using the TruSeq RNA-seq Sample Prep kit according to the manufacturer’s recommended protocols (Illumina, San Diego, USA). The libraries were sequenced for 101 or 151 bp paired-ends on an Illumina HiSeq2500 sequencer using the standard protocol (Illumina).
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bioRxiv - Genomics 2021Quote: ... Library preparation was performed with 100 ng of RNA using a TruSeq Stranded mRNA LT Sample Prep Kit (Illumina, San Diego, USA) according to the manufacturer’s instructions (Part #15031047 Rev ...
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bioRxiv - Genomics 2021Quote: ... Separate libraries were prepared from the two RNA pools using the TruSeq Stranded mRNA LT sample prep kit (Illumina, San Diego, CA). Libraries were run in paired-end 150-base format on both lanes of a dual flow cell in Rapid Run mode on an Illumina HiSeq 2500 System ...
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bioRxiv - Microbiology 2021Quote: ... cDNA libraries were prepared using 100 ng of total RNA according to the manufacturer’s instructions for the Illumina TruSeq Stranded Total RNA Sample Prep Kit with Ribo-Zero Gold (Illumina, San Diego, CA). The concentration and size distribution of the completed libraries were determined using an Agilent Bioanalyzer DNA 1000 chip (Santa Clara ...
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bioRxiv - Systems Biology 2021Quote: We evaluated the main sources of variation on the gene expression matrix to identify and adjust technical confounders (RNA-seq, Affymetrix arrays, Illumina arrays, etc.), study ...
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bioRxiv - Microbiology 2020Quote: ... Construction of the libraries and RNA-Seq analysis were performed by Novogene Corporation (Beijing, China) using an Illumina HiSeq 2000 platform (Illumina, Inc., USA). Raw reads were filtered to remove adaptor and low-quality sequences ...
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bioRxiv - Genomics 2020Quote: ... Samples were prepared separately from each of the three biological replicates of each tissue type using the TruSeqTM RNA Sample Preparation Kits (v2) and sequenced using the Illumina HiSeq 2500 instrument (Illumina Inc., USA) at the Center for Genomic Research and Biocomputing ...
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bioRxiv - Microbiology 2020Quote: ... 2.5 µg of confirmed DNA-free total RNA was treated with Ribo-Zero rRNA Removal Kit for Bacteria according to the manufacturer’s instructions (Illumina, San Diego, CA). Following rRNA removal ...
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bioRxiv - Cancer Biology 2020Quote: ... MCF10A libraries from three biological replicates of each cell type were prepared using NEBNext® Ultra(tm) RNA Library Prep Kit (Illumina®) and sequenced on HiSeq X by Annoroad Gene Technology Ltd ...
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bioRxiv - Molecular Biology 2022Quote: ... 500 ng of purified RNA in each replicate and condition was used for strand-specific RNA-seq library preparation using the Illumina Stranded mRNA Prep kit (Illumina, Cat. 20040534) and IDT Illumina RNA UD Indexes Set B (Illumina ...