Labshake search
Citations for Illumina :
1501 - 1550 of 1653 citations for Recombinant Human Carboxylesterase 2 His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2022Quote: ... Sequencing was performed on the Illumina HiSeq 4000 paired end at 2 X126 bp or single end 126 bp using the TruSeq SBS Kit v4-HS (Illumina, Inc, California, USA).
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bioRxiv - Genomics 2019Quote: ... and 800 bp) and four mate-pair libraries (2, 6, 10, and 20 Kb) following the standard protocols provided by Illumina (San Diego, USA). Subsequently ...
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bioRxiv - Genomics 2022Quote: ... Libraries depleted of ribosomal RNA (figure 2 and 3A-B) were constructed with the TruSeq Total RNA with Ribo Zero plant Kit (Illumina, San Diego, USA), according to the manufacturer’s instructions ...
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bioRxiv - Genetics 2022Quote: ... Illumina compatible P5 P7 sequences were added during post capture amplification and libraries were submitted for 2 × 150bp paired-end next generation sequencing (Illumina, San Diego, CA), targeting 15 million clusters per sample ...
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bioRxiv - Molecular Biology 2019Quote: ... Biomark Access Array system followed by sequencing with 2×225 base pair reads on a MiSeq instrument using version 3 chemistry (Illumina, San Diego, CA). FASTQ files were processed through a custom bioinformatics pipeline (ScanIndel ...
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bioRxiv - Synthetic Biology 2019Quote: ... Kmers shortlisted for testing were selected based on the following cutoffs: abundance in the genome (coverage in combined male and female Illumina data >log10(2), coverage in PacBio data >log10(1) ...
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bioRxiv - Microbiology 2021Quote: Illumina libraries were prepared for paired-end 250-bp sequencing (2 × 250 bp) using the Nextera DNA Flex Library kit (Illumina, San Diego, CA). The libraries were sequenced at the UC Davis Genome Center (Davis ...
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bioRxiv - Microbiology 2020Quote: ... The pool was sequenced on an Illumina MiSeq using the 2 × 250 bp v2 kit with a 10% PhiX control following the manufacturer’s protocol (Illumina, Inc., San Diego, CA) and using custom primers developed from (55) ...
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bioRxiv - Microbiology 2021Quote: Metagenomic libraries (2×150 paired-end) were prepared and sequenced on an Illumina NextSeq 500 sequencing platform (Illumina Inc., San Diego, CA, USA). Quality of the reads and presence of adaptor sequences were checked using FastQC v.0.11.5 (Andrews ...
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bioRxiv - Genomics 2021Quote: ... ~100X sequencing coverage with Illumina short reads was generated on Illumina MiSeq (2 × 300 bp mode) instrument in a high output mode (Illumina, San Diego, CA) to support PacBio read correction and assembly polishing as needed.
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bioRxiv - Evolutionary Biology 2020Quote: ... Barcoded DNA paired end libraries with insert size of approximately 150bp were prepared using Illumina Truseq HT library prep and sequenced on 2 lanes using Illumina NovaSeq (Illumina Inc., California, USA). Raw paired-end reads were checked for quality using FastQC ...
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bioRxiv - Immunology 2021Quote: ... Sequencing was performed in the Human Immunology Core Facility at the University of Pennsylvania using an Illumina 2× 300-bp paired-end kit (Illumina MiSeq Reagent Kit v3, 600-cycle, Illumina MS-102-3003).
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bioRxiv - Genomics 2020Quote: ... The samples were sequenced on the NextSeq 550 platform using paired-end 2×75 bp Reagent Kit from Illumina (San Diego, CA, USA).
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bioRxiv - Microbiology 2021Quote: ... Paired-end (2 × 250 bp) sequencing was performed using MiSeq Reagent Nano kit v2 (500 cycles) on an Illumina MiSeq (Illumina Inc., CA, USA). DNA extraction controls and PCR no template controls were carried through library preparation and sequencing.
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bioRxiv - Genomics 2021Quote: ... Paired-end sequencing (2×75 bases) using MiSeq reaget v2 kit was carried out on Illumina MiSeq (Illumina Inc., San Diego, CA, USA).
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bioRxiv - Genetics 2020Quote: ... and the nuclei was resuspended with Tn5 transposase reaction mix (25◻μl 2◻×◻TD buffer, 2.5◻μl Tn5 transposase and 22.5◻μl nuclease-free water) (Illumina) at 37◻°C for 30 min ...
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bioRxiv - Genomics 2022Quote: ... 2 µL of each P7 and P5 of Nextera XT Index Kit v2 index primers (Illumina Catalogue No. FC-131-2001 to 2004) were added to each well ...
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bioRxiv - Genomics 2022Quote: Libraries were sent to Novogene (Sacramento, CA) for sequencing on a single 2×150 lane of a HiSeq X Ten (Illumina, San Diego CA) and the raw sequencing reads were uploaded to the NCBI Sequence Short Read Archive (SRA ...
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bioRxiv - Genomics 2022Quote: ... spun at 300G for 12 min at 4 ºC and resuspended in 20 uL transposition mix: 1X TD buffer and 2 uL of TDE1 (Illumina #FC-121-1030), 0.01% Digitonin in DMSO ...
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bioRxiv - Genomics 2022Quote: ... and DRB3, 4, 5 (exon 2) genes with Fluidigm Access Array (Fluidigm, Singapore) and sequenced on an Illumina MiSeq sequencer (Illumina, San Diego, USA). HLA alleles and genotypes are called using the Omixon HLA Explore (version 2.0.0 ...
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bioRxiv - Neuroscience 2024Quote: ... Individually barcoded libraries were pooled and sequenced on a NextSeq mid-output paired-end sequencing run at 2×75 using NextSeq 500/550 Mid-Output v2.5 kit (Illumina, San Diego, CA, USA) according to the manufacturer’s instructions ...
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bioRxiv - Evolutionary Biology 2024Quote: ... Samples were 2 × 150 bp paired-end sequenced on one lane each of a HiSeq X Ten instrument (Illumina, Inc., San Diego, CA). Samples for Luke and Nababiep were prepared by HudsonAlpha Institute for Biotechnology (Huntsville ...
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bioRxiv - Evolutionary Biology 2023Quote: ... according to the manufacturer’s instructions and stored in 10 mM Tris buffer at pH 8 and -80 ⁰C until paired-end reads of 2 x 150 bp were sequenced on a NextSeq 500/550 (Illumina, San Diego, CA) at the University of Bristol Genomics Facility ...
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bioRxiv - Physiology 2022Quote: ... The hypervariable regions V3-V4 of the 16S rRNA coding gene were amplified using Illumina primers (fw: CCTACGGGNGGCWGCAG, rev: GACTACHVGGGTATCTAATCC) and sequencing (2 × 300 bp paired-end) was carried out on the MiSeq platform (Illumina, San Diego, California) with a coverage of 50k reads ...
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bioRxiv - Genomics 2023Quote: ... multiplexed at even concentration and sequenced on 600 bp (2 × 300bp) pair-end using Illumina MiSeq platform (Illumina, Inc., USA) (EMP 16S Illumina Amplicon Protocol, n.d.).
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bioRxiv - Molecular Biology 2023Quote: ... The pooled library was sequenced on a MiSeq Illumina platform via 2×150 nt paired-end sequencing using the 300 cycle v2 kit (Illumina, #MS-102-2002).
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bioRxiv - Microbiology 2023Quote: Extracted DNA for all samples was sent to The Center for Applied Genomics (Toronto, Canada) for shotgun metagenome sequencing using an Illumina HiSeq platform with paired 2 × 150 bp reads (Illumina, San Diego, CA). Metagenomic reads were quality trimmed using bbduk in the BBTools suite (https://sourceforge.net/projects/bbmap/ ...
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bioRxiv - Developmental Biology 2023Quote: ... pooled and sequenced on a single lane of an Illumina HiSeq 4000 instrument using a 2×100 paired-end sequencing protocol (Illumina, San Diego, CA) to validate quality and to adjust library pooling according to actual cell capture rates ...
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bioRxiv - Microbiology 2023Quote: ... 2 μl of the plasmid was used as a template and amplified using the Nextera DNA Indexes (Illumina Inc., San Diego, CA, USA) index primers ...
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bioRxiv - Genetics 2023Quote: ... 2 μg of RNA was used as starting material for library construction with TruSeq RNA Sample Prep Kit V2 (Illumina, RS-122-2002) following the manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: ... 16S rRNA gene amplicon libraries targeting the V1-V3 or V4 region were constructed using a dual-indexing method and sequenced on a MiSeq with a 2×300 bp run format (Illumina, San Diego, CA). Reagent-only negative controls were carried through the DNA extraction and sequencing process.
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bioRxiv - Cancer Biology 2023Quote: ... up to 12 samples were pooled equimolar and paired-end reads 2×150bp sequencing on an Illumina NextSeq 500 instrument using the 300 cycle Mid Output v2.5 Reagent Kit (Illumina, San Diego, CA, USA) according to Illumina guidelines ...
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bioRxiv - Immunology 2023Quote: ... which was performed on a MiSeq instrument with paired-end 2×300 bp reads using the NovaSeq 6000 SP Reagent Kit v1.5 (500 cycles) (Illumina, San Diego, California, USA). The protocol was approved by the ethics committee of Liège University Hospital (approval numbers 2021-54 and 2020/107).
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bioRxiv - Genomics 2023Quote: ... Libraries were quantified and sequenced (paired-end, 2 x 111 cycles) using the Illumina NovaSeq 6000 device (Illumina Inc., San Diego, CA, USA) at IPK Gatersleben54 ...
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bioRxiv - Genomics 2024Quote: ... 11 μL Nuclease-free water and 2 μL RP1 and RPIx primers each (10 mM, for details see Illumina smallRNA indexing primer sequences) to the 10 μL single-stranded DNA product ...
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bioRxiv - Microbiology 2024Quote: ... 2 µl of each P7 and P5 of Nextera XT Index Kit v2 index primers (Illumina Catalogue No. FC-131-2001 to 2004) were added to each well ...
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bioRxiv - Microbiology 2024Quote: ... Metagenome sequencing was performed in two lanes of a NovaSeq S4 flow cell in 2×150bp paired-end format on a NovaSeq 6000 (Illumina, San Diego, USA). Raw sequencing data is available on the NCBI SRA (BioProject PRJNA974462).
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bioRxiv - Evolutionary Biology 2021Quote: ... Subpools were then pooled in equimolar conditions and sequenced at Vienna BioCenter Core Facilities (Vienna, Austria) on Illumina® NextSeqTM 550 (2×150bp, Illumina, San Diego, CA). Sequencing was conducted independently for either bait kit.
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bioRxiv - Molecular Biology 2021Quote: Equimolar ratios of the purified amplicons were pooled and subjected to 2 × 250 bp paired-end sequencing on the MiSeq platform (Illumina, San Diego, CA, USA). To increase the genetic diversity ...
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bioRxiv - Genomics 2020Quote: ... Indexed libraries from multiple patients were pooled and sequenced (2 × 150 cycles) using the MiSeq or the NovaSeq systems (Illumina, San Diego, CA, USA). A step-by-step SARS-CoV-2 target enrichment and sequencing protocol is provided in Appendix I.
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bioRxiv - Genomics 2020Quote: ... according to the instructions for qChIP-Seq and paired-end sequencing (2×75nt) was performed on a NextSeq 500/550 using a HighOutput v2 Kit for 150 cycles (Illumina #FC-404-2002, discontinued).
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bioRxiv - Genomics 2020Quote: ... according to the manufacturer’s instructions and paired-end sequencing (2 × 75 bp) was performed on a NextSeq 500/550 using a HighOutput v2 Kit for 150 cycles (Illumina #FC-404-2002, discontinued).
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bioRxiv - Microbiology 2019Quote: ... denatured using 0.1N NaOH followed by a 2min incubation @96C followed by 5min in an ice-water bath just prior to sequencing as per the Illumina protocol for a 2×301 MiSeq run (Illumina, Inc., San Diego, CA). DNA sequence reads from the Illumina MiSeq were demultiplexed and classified in the following manner ...
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bioRxiv - Microbiology 2020Quote: ... Sequencing was performed at the UW-Madison Biotechnology Center using the 2 × 250 bp paired-end method on an Illumina MiSeq following manufacturer’s guidelines (Illumina, Inc., San Diego, CA, USA). Detailed methods about and the library preparation and sequencing can be found in Skarlupka et al ...
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bioRxiv - Systems Biology 2019Quote: ... Samples were equimolar pooled and sequenced by the University of Wisconsin – Madison Biotechnology Center with the MiSeq 2×250 v2 kit (Illumina, San Diego, CA, USA) using custom sequencing primers.
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bioRxiv - Evolutionary Biology 2021Quote: ... Subsequent equimolar pooling of individual libraries from each plate was performed prior to performing large-scale paired-end sequencing (2 × 146 bp) run on the Illumina NovaSeq sequencing system (Illumina, San Diego, CA, USA). The pipeline used to separate the sequencing output of the individual libraries into FASTQ files of 146bp paired-end reads is available on GitHub at https://github.com/czbiohub/utilities.
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bioRxiv - Evolutionary Biology 2021Quote: ... These samples were sequenced (2 × 150 or paired end 150 nt read length) on the Novaseq 6000 platform (Illumina, Inc, San Diego, CA, USA) by the Roy J ...
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bioRxiv - Evolutionary Biology 2020Quote: ... We sequenced paired-end DNASeq and RNASeq reads (2 x 300) at the University of Iowa using the Illumina MiSeq platform (Illumina Inc., San Diego, CA). After inspecting raw read data ...
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bioRxiv - Microbiology 2020Quote: ... The sample was loaded at a concentration of 10 pM and sequenced using 2×250 paired-end strategy on the Miseq (Illumina San Diego, CA, USA) platform for 251 cycles.
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bioRxiv - Plant Biology 2020Quote: ... Quantification of libraries and sequencing (rapid run, paired-end sequencing, 2 × 100 cycles, on-board clustering) using the Illumina HiSeq2500 device (Illumina, San Diego, California, USA) were as described previously 44.