Labshake search
Citations for Illumina :
1451 - 1500 of 1653 citations for Recombinant Human Carboxylesterase 2 His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genomics 2020Quote: ... 2 µl each of P7 and P5 Nextera XT Index Kit v2 index primers (Illumina Catalogue numbers FC-131-2001 to 2004) were added to each well ...
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bioRxiv - Pathology 2019Quote: ... For whole genome sequence analysis isolates were sequenced (2 × 150 bp paired-end) on a MiSeq system (Illumina, San Diego, CA, USA) as previously described (18) ...
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bioRxiv - Microbiology 2019Quote: Tag-encoded 16S rRNA gene amplicon sequencing was performed on a Illumina NextSeq using v2 MID output 2×150 cycles chemistry (Illumina, CA, USA). DNA extraction and library building for amplicon sequencing was performed in accordance to Krych et al ...
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bioRxiv - Plant Biology 2019Quote: ... and submitted for DNA sequencing using the MiSeq Reagent kit v3 with the 2× 300 bp paired-end sequencing protocol (Illumina Inc., USA). A detailed description of the library preparation procedure is included in the Supplemental Methods.
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bioRxiv - Immunology 2019Quote: Single-cell RNA-sequencing libraries were generated using the 10x Genomics Single Cell 3’ Solution (version 2) kit and sequenced to an average depth of > 200k reads/cell (Illumina HiSeq 4000). Data analysis was performed using Python3 pipelines (https://github.com/sansomlab/tenx ...
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Exploring the microbial diversity in oil-contaminated mangrove sediments using 16S rRNA metagenomicsbioRxiv - Microbiology 2019Quote: ... Paired-end sequencing libraries (2 × 300 bp) were prepared using the Illumina Nextera XT Library Preparation Kit (Illumina Inc., San Diego, CA) and sequenced on the Illumina MiSeq platform using V3 chemistry according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2021Quote: ... and PCR and sequencing were performed as described previously (2 x 250bp paired-end reads, on an Illumina Miseq (Lemont, IL, USA)) [68–70] ...
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bioRxiv - Cancer Biology 2021Quote: ... Strand-specific libraries were generated using the TruSeq Stranded mRNA samples preparation kit (illumine Inc., San Diego, RS-122-2101/2) according to manufacturer’s instructions (Illumina, part #15031047 Rev.E). Briefly ...
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bioRxiv - Microbiology 2021Quote: ... The sequencing data were generated with paired-end sequencing (2 × 300 nucleotides) on the MiSeq platform (Illumina, Inc., San Diego, CA, USA).
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bioRxiv - Genomics 2020Quote: ... Up to 96 AOIs were pooled and sequenced on a NextSeq High Output v2.5 (75 cycles, 2×38 bp; Illumina, cat. no. 20024906).
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bioRxiv - Genomics 2020Quote: ... we ran the library on a 2×100 cycle sequencing run on an Illumina MiSeq v2 (Illumina, Inc., San Diego, CA, USA). The qualified library was sequenced using the Illumina NovaSeq6000 (Illumina ...
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bioRxiv - Microbiology 2022Quote: ... and variant calling as described by the software manufacturer (https://help.geneious.com/hc/en-us/articles/360045070991-Assembly-of-SARS-CoV-2-genomes-from-tiled-amplicon-Illumina-sequencing-using-Geneious-Prime). For each sample ...
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bioRxiv - Microbiology 2022Quote: ... in a 100 µl reaction with 2 µl gDNA (∼100 ng) and primers oJMP697 and oJMP698 (nested primers with adapters for index PCR with Illumina TruSeq adapter) according to the manufacturer’s protocol using a BioRad C1000 thermalcycler with the following program ...
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bioRxiv - Microbiology 2022Quote: ... the constructed DNA libraries were sequenced with a 2 × 150 bp read length using the NextSeq 500 Sequencing System (Illumina, CA, USA).
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bioRxiv - Plant Biology 2023Quote: ... The nuclei pellet was then immediately resuspended in 25 μ L of 2x tagmentation buffer containing 2 μ L of TDE1 (Illumina). The reaction was placed at 37C for 30 minutes with gentle mixing three times ...
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bioRxiv - Evolutionary Biology 2023Quote: ... insert size 550 nt) were constructed from 1.8-2 µg gDNA with the TruSeq DNA PCR-Free kit (Illumina, San Diego, CA) and sequenced on the Illumina HiSeq 2500 by the Genomics Core Facility at Pennsylvania State University ...
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bioRxiv - Microbiology 2024Quote: ... Equal amounts of DNA from each library (except xGen-prepared) were pooled and sequenced using 2×150 bp paired-end settings on an Illumina NextSeq550 platform (Illumina, CA, USA). The xGen-prepared libraries were sequenced separately on an iSeq100 System (Illumina ...
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bioRxiv - Microbiology 2023Quote: ... The prepared library was sequenced using a MiSeq sequencing system with a V3 reagent kit (300 × 2 bp; Illumina, 4–6 samples). Lake Biwa viral contigs/genomes (LBVs ...
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bioRxiv - Microbiology 2023Quote: ... and finally sequenced with phiX as control using paired-end 2×250-bp chemistry on the Illumina MiSeq 2000 platform (Illumina Inc., USA)
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bioRxiv - Microbiology 2023Quote: ... The quantified and denatured libraries were sequenced using paired-end 2×250-bp chemistry with 5% PhiX as an internal control on the Illumina MiSeq 2000 platform (Illumina Inc., USA). The quality-filtered reads were de-novo assembled using SPAdes (version 3.11.1) ...
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bioRxiv - Cell Biology 2023Quote: The libraries are sequenced in paired-end (2 x 150 cycles) through the SBS (sequencing by synthesis) technology using NovaSeq® 6000 (ILLUMINA®).
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bioRxiv - Genomics 2023Quote: ... 6.3 Gbp of data in 41.8 million reads (2 × 150 cycles) were produced using the MiniSeq System (Illumina, San Diego, Ca, USA). Illumina short reads were quality checked using FastQC v0.11 (http://www.bioinformatics.babraham.ac.uk/projects/fastqc) ...
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bioRxiv - Systems Biology 2023Quote: ... in a 100 µl reaction with 2 µl gDNA (∼100 ng) and primers oJMP697 and oJMP698 (nested primers with adapters for index PCR with Illumina TruSeq adapter) according to the manufacturer’s protocol using a BioRad C1000 thermocycler with the following program ...
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bioRxiv - Genetics 2023Quote: ... in a 100 µl reaction with 2 µl gDNA (∼100 ng) and primers oJMP697 and oJMP698 (nested primers with partial adapters for index PCR with Illumina TruSeq adapter) according to the manufacturer’s protocol using a BioRad C1000 thermal cycler with the following program ...
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bioRxiv - Microbiology 2023Quote: ... Sequencing was conducted on an Illumina MiSeq using a paired-end 2 x 300 base-pair method (Illumina, San Diego, CA, USA).
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bioRxiv - Microbiology 2023Quote: ... The resulted pool of normalized DNA was cleaned one last time using magnetic beads at a ratio of 0.65:1 and thereafter sequenced at a commercial company (Baseclear, Leiden, The Netherlands) on the Illumina MiSeq platform using 2 × 300 bp paired-end sequencing (Illumina MiSeq PE300). Three negative control samples were included to detect possible contamination during library preparation and sequencing ...
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bioRxiv - Genomics 2023Quote: ... These five tissue-specific samples were subjected to Illumina’s TruSeq Stranded mRNA library preparation kit and sequenced on NovaSeq 6000 (Illumina, 2×150 bp). For long-read RNA sequencing ...
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bioRxiv - Microbiology 2023Quote: ... cDNA was amplified using ARTIC v4 primer pools (https://github.com/artic-network/artic-ncov2019) and variant calling results were generated using the SIGNAL (SARS-CoV-2 Illumina GeNome Assembly Line) pipeline v1.5.0 42 ...
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bioRxiv - Cell Biology 2023Quote: ... and 2×150 bp paired-end sequencing was performed at a depth of approximately 400 million reads (HiSeq; Illumina, San Diego, USA). Cellranger (Version 3.0.2 ...
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bioRxiv - Genomics 2023Quote: ... All libraries were pooled and subjected to paired-end 75 bp sequencing (paired- end 76 nt reads with the first 1 nt of Read 1 and the last 1 nt of Read 2 trimmed) using the NextSeq500 system (Illumina, CA, USA). For each library ...
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bioRxiv - Cancer Biology 2023Quote: ... Bacterial 16S rRNA gene V1-V2 region was amplified using 27F and 338R PCR primers and sequenced on a MiSeq (2×250 bp; Illumina, Hayward, CA). Bioinformatic processing and statistical analysis were performed in R software environment as described previously 62 ...
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bioRxiv - Microbiology 2023Quote: ... Sequencing was conducted on an Illumina MiSeq using a paired-end 2 x 300 base-pair method (Illumina, San Diego, CA, USA). We estimated this should provide a mean of 60 000 reads per sample ...
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bioRxiv - Immunology 2024Quote: ... MHC class I genotyping was performed using genomic DNA isolated from PBMCs by sequencing a 150 bp region of exon 2 (Illumina MiSeq system). Sequences were analyzed by comparison to an inhouse database as previously described49 ...
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bioRxiv - Molecular Biology 2021Quote: ... and 2.5 µl of each Nextera Index 1 (N7XX) and Nextera Index 2 (N5XX) primers from a Nextera DNA Sample Preparation Index Kit (Illumina, FC-121-1011). PCR was performed according to the following protocol ...
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bioRxiv - Microbiology 2019Quote: ... 2 µL of each P7 and P5 of Nextera XT Index Kit v2 index primers (Illumina Catalogue No. FC-131-2001 to 2004) were added to each well ...
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bioRxiv - Neuroscience 2019Quote: ... Libraries were sequenced in a paired-end 150 bp (2×150bp) configuration on the NextSeq 500 Illumina platform (Illumina, San Diego, CA, USA). Raw reads were processed and analyzed on the Stampede Cluster at the Texas Advanced Computing Facility (TACC) ...
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bioRxiv - Microbiology 2019Quote: ... Then applicants were pooled into equimolar quantities and subjected to high-throughput sequencing using Miseq Benchtop Sequencer for 2 × 300 bp pair-end sequencing (Illumina, San Diego, CA). Databases of EzBioCloud and Central Bureau of Fungal Cultures (CBS-KNAW ...
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bioRxiv - Plant Biology 2019Quote: ... Libraries for RNA expression analysis were prepared in duplicates from 2 μg of total RNA using the TrueSeq Stranded mRNA Sample Prep Kit (Illumina, San Diego, CA) following the manufacturer’s instructions ...
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bioRxiv - Genomics 2019Quote: ... V3-V4 16S rRNA was amplified from total genomic DNA extracted from the swab and sequenced (2 × 300bp) on an Illumina MiSeq (Illumina, San Diego, CA). 16S rRNA bacterial sequence reads were assessed for quality and analyzed using phylogenetic and Operational Taxonomic Unit (OTU ...
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bioRxiv - Genomics 2021Quote: ... Whole-genome sequencing data were generated using (i) an Illumina NextSeq-500 platform with a 2 × 150 nt paired-end protocol (Nextera XT library, Illumina, San Diego, CA); and (ii ...
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bioRxiv - Immunology 2021Quote: ... 62 samples including the mock community were pooled at equimolar concentrations and the library was sequenced using a MiSeq sequencer with 2 x 250 bp paired-end run (Illumina MiSeq, v2 kit). The resulting sequence data was processed using ‘Quantitative insights into microbial ecology 2’ (QIIME2 version 2020.2 ...
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bioRxiv - Plant Biology 2020Quote: ... followed by paired end sequencing (100bp x 2) on a HiSeq2000 with a HiSeq Paired-End Cluster Generation Kit and HiSeq Sequencing Kit (Illumina, San Diego, U.S.A.). Trimmomatic (ver ...
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bioRxiv - Microbiology 2020Quote: ... 30 μL of the 20 nM library was diluted to 500 uL using Illumina buffer HT1 and loaded onto a 500 cycle MiSeq (version 2) flowcell (Illumina, MS-102-2003). Paired-end run cycle parameters were 260 (Read1 ...
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bioRxiv - Plant Biology 2021Quote: ... Prepared library was loaded into Illumina platform for cluster generation and sequencing was performed using paired-end (PE) 2×150 bp library on Illunima platform (Illumina TruSeq RNA 500) (Bolger et al. ...
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bioRxiv - Plant Biology 2021Quote: ... Paired-end (2 × 150 bp) sequencing of the cDNA libraries was performed on the Illumina HiSeq 2000 (Illumina Inc., San Diego, CA, USA).
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bioRxiv - Microbiology 2021Quote: ... Goe13 and lysogens and sequenced with the MiSeq system and reagent kit V.3 (2 x 300 bp) (Illumina, San Diego, CA, USA) and the NovaSeq system (2x 150bp ...
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bioRxiv - Microbiology 2021Quote: Paired-end DNA and cDNA libraries (2 × 150 bp) were constructed using TruSeqTM DNA Sample prep kit (Illumina Inc., San Diego, CA, USA) and TruSeqTM RNA Sample prep kit (Illumina Inc. ...
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bioRxiv - Microbiology 2021Quote: ... Each pool of V4-V5 16S rRNA amplicons was sequenced (2×300nt paired end) on one lane of a MiSeq V2 sequencer (Illumina, San Diego, CA) at the Carver Biotechnology Center of the University of Illinois ...
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bioRxiv - Microbiology 2021Quote: ... All 24 samples were multiplexed together into a single High Output 2×75bp run on a NextSeq 500 instrument (Illumina, San Diego, CA). Raw reads were mapped to the C ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... one RNA pool of 5 livers from each treatment and sex were independently subjected to sequencing (Illumina Novaseq 6000 paired-end (2×150)) ...