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Citations for Illumina :
101 - 150 of 5691 citations for TD 5108 CAS 866933 46 2 99% since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2023Quote: ... and nuclei were resuspended in 200μl transposition mix (1x TD buffer containing X µl of Illumina Tagment DNA TDE1 Enzyme per every X nuclei) ...
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bioRxiv - Immunology 2023Quote: ... 3mM MgCl2) on ice and resuspended in 10μl of transposition mix (1X TD Buffer from Illumina, Transposase from Illumina ...
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bioRxiv - Microbiology 2024Quote: ... About 28ng of gDNA of each sample was tagmented using TD buffer and TDE1 enzyme (Illumina) and incubated in a thermocycler at 55°C for 5min prior to melting at 95°C for 0.5min ...
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bioRxiv - Genetics 2024Quote: ... Tagmentation was performed directly on spheroplasts by mixing 1µL of spheroplasted cells with 0.25µL of TDE1 and 1.25µL of TD buffer (enzyme and buffer from Illumina) and incubating at 55°C for 15 minutes ...
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bioRxiv - Cancer Biology 2021Quote: ... Each well contained 10 μL tagmentation buffer (5 μL NIB and 5 μL TD buffer from Illumina). For the second sort plate ...
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bioRxiv - Genetics 2021Quote: ... The pellet was then resuspended in the tagmentation reaction mix (25 μL 2X TD Buffer (Illumina, 15027866), 2.5 μL TD Enzyme (Illumina ...
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bioRxiv - Neuroscience 2023Quote: ... Pellets were resuspended in transposase reaction mix (25 μL 2x TD Buffer (Illumina Cat #FC-121-1030) 2.5 μL Tn5 Transposase (Illumina Cat #FC-121-1030 ...
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bioRxiv - Evolutionary Biology 2023Quote: ... The pellets were resuspended in the reaction buffer (25 μL TD Buffer (Illumina Cat #FC-121-1030), 2.5 μL Tn5 Transposes (Illumina Cat #FC-121-1030) ...
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bioRxiv - Cancer Biology 2023Quote: ... Fixed cells were transposed using barcoded Tn5 (Seqwell) in a transposition buffer (1 × TD buffer from Illumina Nextera kit ...
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bioRxiv - Neuroscience 2023Quote: ... the nuclei were extracted and resuspended with the transposase reaction mix (25 μl 2x TD buffer (Illumina); 2,5 μl Transposase (Illumina) ...
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bioRxiv - Molecular Biology 2023Quote: ... with 25 μl of 2x TD buffer and 2.5 μl tagmentation enzyme (Illumina transposase FC-121-1030). DNA was purified using a Qiagen mini elute kit following the manufacturer’s instructions ...
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bioRxiv - Immunology 2024Quote: ... Nuclei were immediately resuspended in 25 μL of the transposition reaction mix (12.5μL 2x TD Buffer (Illumina), 1.25μL Tn5 Transposases ...
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bioRxiv - Genomics 2023Quote: ... resuspended in transposition buffer (TD buffer, Tn5 Transposase from the Illumina Tagment DNA Enzyme and Buffer kit) and incubated for 30 min at 37°C ...
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bioRxiv - Developmental Biology 2023Quote: ... and library preparation for transcriptome profiling (Novozyme, TruePrep DNA Library Prep Kit V2 for Illumina, TD-503). Next generation sequencing was performed on the Illumina Hiseq 2500 or Novaseq platform (Berry Genomics).
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bioRxiv - Genetics 2024Quote: ... The nuclei were resuspended in transposition reaction mix (2x TD Buffer (Illumina Cat #FC-121–1030, Nextera), 2.5µl Tn5 Transposase (Illumina ...
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bioRxiv - Genomics 2024Quote: ... nuclei were recovered with 2.5 μL of 2X TD buffer and 2.5 μL transposase (TDE1) mixture (Illumina). Following mixing by pipetting ...
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bioRxiv - Evolutionary Biology 2022Quote: ... High-throughput genome sequencing was performed either on Hiseq 2000 (2 × 100 or 2 × 150 paired-end reads) or Miseq (2 × 300 paired-end reads) sequencer (Illumina, San Diego, CA) following the manufacturer ‘s instruction.
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bioRxiv - Physiology 2021Quote: ... High-throughput genome sequencing was performed on a HiSeq 2500 (2 × 100 or 2 × 150 paired-end reads) or MiSeq (2 × 300 paired-end reads) sequencer (Illumina, San Diego, CA), following the manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: ... version 3 (2×300 bp read length; Illumina, San Diego, CA, USA).
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bioRxiv - Genomics 2023Quote: ... the Illumina TruSeq RNA sample preparation kit v.2 (Illumina, CA, USA) was used for library preparation following the low-throughput protocol ...
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bioRxiv - Microbiology 2020Quote: ... The sorted cells were then washed with phosphate buffered saline (PBS) and resuspended in 50ul TD buffer (Illumina) with 0.01% digitonin and 2.5ul TDE Tagmentation enzyme (Illumina) ...
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bioRxiv - Genomics 2020Quote: ... The resulting nuclei were resuspended in 50µl of transposition reaction buffer (25µl of 2x TD buffer from the Nextera kit, Illumina; 2.5µl of Tn5 transposase enzyme from the Nextera kit ...
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bioRxiv - Genetics 2021Quote: ... The nuclei were resuspended in the transposition reaction mix (2x TD Buffer (Illumina Cat #FC-121-1030, Nextera), 2.5⍰μl Tn5 Transposase (Illumina Cat #FC-121-1030 ...
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bioRxiv - Neuroscience 2021Quote: ... Nuclei were then resuspended in 50 ul 1x TD Buffer containing 2.5 ul Tn5 enzyme from Nextera (Illumina) and incubated for 30 min at 37°C ...
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bioRxiv - Immunology 2020Quote: ... the pellet was resuspended in 50 μL ATAC reaction mix (25uL 2X TD buffer, 2.5 μL Nextera Enzyme, 22.5 μL water, Illumina). The transposase reaction was carried out at 37°C for 30 minutes ...
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bioRxiv - Molecular Biology 2021Quote: ... The resulting pellet was resuspended in transposase reaction mix (25μl cold lysisl 2x TD buffer, 10μl cold lysisl transposase (Illumina), 15μl cold lysisl nuclease-free H2O ...
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bioRxiv - Genomics 2023Quote: ... DNA was tagmented by the addition of 10 µL of tagmentation mix (0.01 µL of a custom TDE1 enzyme in 9.99 µL of 2x Nexterda TD buffer, Illumina) and plates incubated at 55°C for 5 minutes ...
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bioRxiv - Neuroscience 2023Quote: ... Nuclei were resuspended in 25uL of transposition mix (12.5ul 2x TD buffer, 1.25ul transposase (Illumina, catalog nr 20034197), 8.25ul PBS ...
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bioRxiv - Developmental Biology 2023Quote: ... Pelleted nuclei were resuspended in 50 μl of cold transposition buffer (TD Buffer, 100 nM Tn5 (Illumina, 20034197), 20% PBS ...
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bioRxiv - Developmental Biology 2022Quote: ... The cell pellet was then re-suspended in 50ml of transposition reaction mix (25ml 2x TD buffer (Illumina), 2.5ml transposase (Illumina) ...
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bioRxiv - Immunology 2024Quote: ... and nuclei were resuspended in 50 μl of transposition mix (25 μl TD Tagment DNA Buffer (Illumina, 15027866), 2.5 μl TDE1 Tagment DNA Enzyme (Illumina ...
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bioRxiv - Immunology 2024Quote: ... resuspended in 50 μl transposition reaction mixture (25 μl TD buffer, 2.5 μl TDE1, 22.5 μl ddH2O) (20034197, Illumina), and incubated at 42°C for 45 min ...
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bioRxiv - Genetics 2021Quote: ... The pellet was resuspended in 10 µL of transposition mixture (5 µL TD buffer, 3.2 µL PBS, 0.89 µL Tn5 (Illumina, 20034197), 0.1% Tween-20 ...
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bioRxiv - Developmental Biology 2021Quote: ... The nuclei pellet was then resuspended in the 50ul of the transposition reaction mix (25ul Tagment DNA buffer, 2.5ul TD Tn5 Transposase enzyme, 22.5ul nuclease-free water) (Illumina; 20034197). The transposition reaction was incubated at 37°C for 30 min on a thermomixer with constant gentle shaking at 1000 rpm ...
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bioRxiv - Molecular Biology 2021Quote: ... The nuclei were pelleted and resuspended in 1x TD with 2.5 l TDE1 (Nextera DNA library prep kit, Illumina). These were incubated for 30 min at 37oC ...
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bioRxiv - Genetics 2020Quote: ... were incubated in a 50 µl reaction with 0.8 µl of Tn5 transposase at 1× TD buffer from the Nextera library preparation kit (Illumina) for 5 min at 55°C ...
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bioRxiv - Cell Biology 2020Quote: ... The pellet was resuspended in transposase reaction mix (25 uL 2X TD buffer, 2.5 μL Transposase (Nextera DNA sample preparation kit, Illumina), and 22.5 μL H20 and incubated at 37°C for 30 min ...
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bioRxiv - Molecular Biology 2022Quote: ... Nuclei pellets were resuspended gently in transposition reaction mix (25 µL 2X TD Buffer [Illumina Cat #FC-121-1030], 2.5 µL transposase [Illumina Cat #FC-121-1030] and 22.5 µL nuclease-free water ...
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bioRxiv - Cell Biology 2022Quote: ... Nuclei pellets were then resuspended in 50 µl of transposition mix (2.5 µl Tn5 transposase, 25 µl 2x TD buffer (both Illumina), 0.5 µl 1% Digitonin (Promega) ...
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bioRxiv - Genomics 2022Quote: ... Supernatant was removed and transposition reaction was set up with 25 μl TD (2x reaction buffer, Nextera Kit, Illumina), 2.5 μl TDE1 (Tn5 transposase ...
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bioRxiv - Genomics 2021Quote: ... We collected nuclei by centrifuging at 500 g at 4°C and resuspended nuclei in 5 ul TD buffer with 2.5 ul Tn5 enzyme (Illumina Tagment DNA TDE1 Enzyme and Buffer Kits) ...
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bioRxiv - Genetics 2022Quote: ... Nuclei pellet was obtained by centrifugation at 500g for 5 minutes at 4 degrees and resuspended in 100μl ice-cold 1x TD buffer (20034198, Illumina). About 10K nuclei was used for transposition reaction at 37 degrees for 30 minutes in a thermomixer ...
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bioRxiv - Synthetic Biology 2021Quote: ... cDNA was diluted to an average of 200 pg/µl and 100 pg cDNA from each cell was tagmented by adding 1 µl TD and 0.5 µl ATM from a Nextera XT DNA Library Preparation Kit (Illumina) to 0.5 µl diluted cDNA in each well of a fresh 384-well plate ...
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bioRxiv - Cell Biology 2021Quote: ... The pellet was resuspended in transposase reaction mix (25 µL 2X TD buffer, 2.5 µL Transposase (Nextera DNA sample preparation kit, Illumina), and 22.5 µL water and incubated at 37°C for 30 min ...
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bioRxiv - Genomics 2020Quote: ... The supernatant was discarded and cells were resuspended directly in Dig-transposition buffer (25 μl 2x TD Buffer [Illumina] ...
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bioRxiv - Genomics 2021Quote: ... and 5K samples were resuspended in 50 μl, 10 μl, and 5 μl of transposition mix (25 μl 2x TD buffer, 2.5 μl Tn5 (Illumina), 16.5 μl PBS (Invitrogen) ...
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bioRxiv - Genomics 2023Quote: ... about 40 ng purified pre-amplified Hi-C sample was fragmented in two 50 μl tagmentation mix (1x TD buffer and 0.5 μl TDE1 (Illumina Tagment DNA TDE1 Enzyme and Buffer Kit ...
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bioRxiv - Developmental Biology 2024Quote: ... The cell pellet was resuspended in transposition reaction mix (12.5 μl TD-Buffer, 1.25 μl Tn5, 11.25 μl water) (Nextera DNA Library Prep Kit, Illumina, Cat# 15028212) and incubated for 30 min at 37°C ...
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bioRxiv - Developmental Biology 2024Quote: ... Pellets were resuspended in 50 µL of transposition reaction (2X TD buffer, 1X PBS, 0.1% Tween-20, 0.1% Digitonin, 5µL of Illumina Tn5 transposase) and incubated for 30 min at 37°C with 1,000 rpm agitation ...
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A gene desert required for regulatory control of pleiotropic Shox2 expression and embryonic survivalbioRxiv - Developmental Biology 2023Quote: ... 50’000 nuclei were then pelleted at 500 RCF for 10 min at 4°C and resuspended in 50 pL transposition reaction mix containing 25 pL Nextera 2x TD buffer and 2.5 pL TDE1 (Nextera Tn5 Transposase; Illumina) (cat ...