Labshake search
Citations for Illumina :
101 - 150 of 667 citations for Red colored Polystyrene Beads 1 um since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genomics 2021Quote: ... Direct tagmentation of the mRNA/cDNA hybrids and PCR amplification were performed on beads using Nextera XT DNA Library Prep Kit (Illumina); the Reference Guide was followed exactly for 20K and 10K samples ...
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bioRxiv - Biochemistry 2022Quote: ... The PCR products were purified with magnetic-beads (Berkeley Sequencing Core Facility) before being subjected to next generation sequencing (NGS) with MiSeq (Illumina) at 2×300bp with a depth of at least 10,000 reads per sample.
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bioRxiv - Cell Biology 2022Quote: ... RNA samples were enriched for mRNA using poly-A attached magnetic beads followed by Paired-end RNA-seq library preparation using the TruSeq RNA Sample Prep kit (Illumina). One hundred nucleotides were sequenced using the HiSeq platform (Illumina ...
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bioRxiv - Molecular Biology 2022Quote: ... We omitted the final elution step as we carried out the cDNA synthesis on the magnetic beads using ScriptSeq v2 RNA-Seq Library Preparation Kit (Illumina). The libraries were size selected using BluePippin DNA size selection system (Sage Science) ...
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bioRxiv - Developmental Biology 2022Quote: ... Indexed libraries were prepared using the Chromium Single Cell 3’ Library & Gel Bead Kit v2 (10X Genomics; PN-120237) and sequenced on a NovaSeq 6000 (Illumina) to obtain a sequencing depth of 1.50E+08 paired-end reads per library ...
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bioRxiv - Genetics 2024Quote: ... The libraries were purified by 0.5X-1.5X volumes left-right side of Ampure XP DNA purification beads and sequenced in 50-bp single-end mode with HiSeq 2500 sequencing technology (Illumina), per the manufacturer’s recommendations.
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bioRxiv - Molecular Biology 2024Quote: ... AMPure beads (Beckman Coulter, A63880 were used to remove primer dimers and libraries were sequenced on a NextSeq 500 instrument (Illumina) to generate 75 bp single-end reads.
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bioRxiv - Bioengineering 2023Quote: ... The PCR products were purified with magnetic beads (Berkeley Sequencing Core Facility) before being subjected to next-generation sequencing (NGS) with MiSeq (Illumina) at 2×300 bp with a depth of at least 20,000 reads per sample ...
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bioRxiv - Cancer Biology 2023Quote: ... Illumina indexed adapters were added to the DNA on the M-280 beads using the TruSeq Library Sample Preparation kit (Illumina) as follows ...
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bioRxiv - Neuroscience 2023Quote: RNA samples were enriched for mRNA through bead-based polyA selection and libraries were generated with the NEBNext Ultra RNA Library Prep Kit (Illumina). cDNA libraries were barcoded and sequenced together on an Illumina Hiseq 4000 sequencer ...
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bioRxiv - Developmental Biology 2023Quote: ... 2 μl amplified cDNA was tagmented in 1x Tagmentation Buffer using 0.8 μl bead-linked transposome (Illumina DNA Prep, (M) Tagmentation ...
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bioRxiv - Bioengineering 2023Quote: ... The PCR products were purified with magnetic beads (Berkeley Sequencing Core Facility) before being subjected to next-generation sequencing (NGS) with MiSeq (Illumina) at 2x300 bp with a depth of at least 20,000 reads per sample ...
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bioRxiv - Genomics 2023Quote: ... The sequencing libraries were generated on the streptavidin C1 beads using Illumina Tagment DNA Enzyme and Buffer kit (Illumina, 20034198) and run on the Illumina Novaseq S4 flowcell.
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bioRxiv - Cancer Biology 2023Quote: Bead-purified cDNA pools were used for tagmentation-based library preparation with a Nextera XT DNA Library Preparation Kit (Illumina) using a custom PCR amplification strategy to generate 3’ biased libraries containing oligodT cell barcodes as previously published,60 with some modifications ...
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bioRxiv - Neuroscience 2024Quote: ... DNA libraries suitable for sequencing were prepared from 300-500 ng of total RNA with oligo-dT capture beads for poly-A-mRNA enrichment using the TruSeq Stranded mRNA Library Preparation Kit (Illumina) according to manufacturer’s instructions (1/2 volume) ...
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bioRxiv - Neuroscience 2024Quote: ... DNA libraries suitable for sequencing were prepared from 500 ng of total RNA with oligo-dT capture beads for poly-A-mRNA enrichment using the TruSeq Stranded mRNA Library Preparation Kit (Illumina) according to manufacturer’s instructions (optimized for ½ volume reactions) ...
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bioRxiv - Zoology 2024Quote: ... and the complementary DNA libraries were constructed using magnetic beads and Illumina’s NEBNext Ultra RNA Library Prep Kit (Illumina, USA). The RNA sequencing was conducted on an Illumina platform ...
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bioRxiv - Immunology 2024Quote: ... libraries were purified with selection beads and sequenced using Illumina MiSeq 2X150 paired-end read length (MS-102-2002) (Illumina). The TCR sequence FASTQ files were uploaded to iRepertoire bioinformatics website for analysis ...
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bioRxiv - Neuroscience 2024Quote: ... The 10x Genomics Chromium Single Cell 3’ Library and Gel Bead Kit v3 were used to construct scRNA-seq libraries and sequencing was conducted on the NovaSeq 6000 instrument (Illumina). Raw scRNA-seq data have been deposited within the Genome Sequence Archive for Human (https://ngdc.cncb.ac.cn/gsa-human/ ...
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bioRxiv - Cell Biology 2024Quote: ... Sequencing adapters were added to the bead-bound DNA by tagmentation with the Tagment DNA Enzyme and Buffer Kit (Illumina). After washes ...
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bioRxiv - Developmental Biology 2024Quote: ... 2 µL amplified cDNA was tagmented in 1X Tagmentation Buffer using 0.8 µL bead-linked transposome (Illumina DNA Prep, (M) Tagmentation ...
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bioRxiv - Cancer Biology 2020Quote: ... Samples were cleaned using Sample Purification Beads from the TruSeq Methyl Capture EPIC LT Library Prep Kit (Illumina, #FC-151-1002) according to the manufacturer’s instructions with modifications ...
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bioRxiv - Genetics 2022Quote: ... for further sample processing and measurement of DNAm on the Illumina Infinium Methylation EPIC Bead-Chip platform (Illumina, San Diego, USA). Extensive details regarding the quality control (QC ...
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bioRxiv - Plant Biology 2024Quote: ... DNA eluted from Chromatin-immunoprecipitated was prepared for library construction by using NEBNext® Ultra™ II DNA Library Prep with Sample Purification Beads according to standard operating procedures and then sequenced using HiSeq2000 (Illumina). Paired-end raw reads were first mapped to the Arabidopsis genome (TAIR10 ...
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bioRxiv - Genetics 2024Quote: The process of library construction involved the purification of messenger RNA from total RNA using magnetic beads attached to poly-T oligos (TruSeq RNA Sample Prep Kit v2, Illumina Inc.). Subsequently ...
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bioRxiv - Genomics 2023Quote: ... DNA was cleaned by AMPure XP beads (Agentcourt, USA) and tagmented and indexed using Nextera XT Index Kit (Illumina, Inc., USA). Clean DNA was again quantified and evaluated using Qubit (Invitrogen ...
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bioRxiv - Genetics 2023Quote: ... Enriched DNA fragments were then cleaned up using AMPure XP beads and final DNA libraries eluted in 30 uL of RSB (Illumina #20020599).
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Activation of glucocorticoid receptor signaling inhibits KSHV-induced inflammation and tumorigenesisbioRxiv - Cancer Biology 2023Quote: ... mRNA was isolated using Oligo(dT) beads and library was prepared following the Illumina TruSeq Stranded mRNA Library Prep Kit (Illumina, 20020594). Sequencing was carried out using Illumina HiSeq 3000 with the 50-bp single-read sequencing module ...
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bioRxiv - Genomics 2024Quote: ... Sequencing adapters were added to the bead-bound DNA by Tagmentation with the Illumina Tagment DNA Enzyme and Buffer Kit (Illumina, 20034197). After washes ...
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bioRxiv - Molecular Biology 2020Quote: ... pooled and then purified using AMPure beads before running index-PCR using the Nextera XT Index Kit (Illumina, San Diego, CA, USA). AmpliSeq libraries were subsequently normalized before sequencing the libraries as 300bp paired-end reads on Illumina MiSeq (Illumina ...
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bioRxiv - Cancer Biology 2022Quote: ... using MiSeq V2 300 cycle reagent kit after the amplified library was purified with AMPure XP beads (Beckman Coulter, CA, USA) (Illumina, CA, USA) (10).
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bioRxiv - Evolutionary Biology 2022Quote: ... The final purified (AMPure XP beads) product was then quantified using qPCR according to the qPCR Quantification Protocol Guide (KAPA Library Quantification kits for Illumina Sequecing platforms) and qualified using the TapeStation D1000 ScreenTape (Agilent Technologies ...
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bioRxiv - Immunology 2024Quote: ... PCR product purification was purified with AMPure XP magnetic beads and used for second PCR with supplied primers and IDT® for Illumina® DNA/RNA UD Indexes (Illumina). After 10-11 PCR cycles ...
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bioRxiv - Cell Biology 2022Quote: ... using poly-T oligos attached to magnetic beads and converted to sequence-ready libraries using the TruSeq stranded mRNA sample prep protocol (cat. #: RS-122-2101, Illumina, San Diego, CA). The final libraries were validated on the bioanalyzer DNA chips ...
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bioRxiv - Cell Biology 2020Quote: Infinium Methylation EPIC Bead Chip assay genome-wide DNA methylation profiles of HLFs were generated using Illumina’s Infinium Methylation EPIC Bead Chip assay (EPIC Array) (Illumina, San Diego, CA, USA) at the Genomics and Proteomics Core Facility at DKFZ (Heidelberg ...
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bioRxiv - Neuroscience 2023Quote: ... mRNA from eukaryotic organisms was enriched using oligo(dT) beads and then rRNA was removed using the Ribo-Zero rRNA Removal Kit (Illumina, San Diego, CA, USA). First ...
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bioRxiv - Genomics 2020Quote: ... transcripts were purified from total RNA using oligo(dT) magnetic beads and mRNA sequencing libraries were prepared with the TruSeq Stranded mRNA Library Preparation Kit (Illumina, RS-122-2101, RS122-2102). A D1000 Screen Tape assay (Agilent ...
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bioRxiv - Cell Biology 2021Quote: ... transcripts were purified from total RNA using oligo(dT) magnetic beads and mRNA sequencing libraries were prepared with the TruSeq Stranded mRNA Library Preparation Kit (Illumina, RS-122-2101, RS122-2102) (9).
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bioRxiv - Genetics 2022Quote: ... Ancient individuals and present-day individuals (most genotyped on the HO SNP array, and some also genotyped on the Illumina Human Omni5 Bead Chip [28]) were projected into the three-dimensional (3D ...
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bioRxiv - Microbiology 2023Quote: ... Size selection and purification of the metagenomic libraries was carried out using SparQ PureMag beads (Quantabio, Beverly, MA, USA) and an Illumina Genome Analyzer (Illumina Inc., San Diego, CA, USA) with a KABA SYBR Fast Universal qPCR Kit (Kapa Biosystems ...
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bioRxiv - Cancer Biology 2020Quote: ... The PCR-amplified cDNA was purified using AMPure XP beads and processed with the Nextera XT DNA Library Preparation Kits (Illumina, Cat. # FC-131-1024 and FC-131-1096) as per the protocol provided by the manufacturer.
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bioRxiv - Cell Biology 2024Quote: ... were pooled at a 1:1:1:etc ratio before sequencing on an Illumina NextSeq 500/550 instrument using a version 2 kit (Illumina). Fastq files were generated for each library in Illumina BaseSpace ...
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bioRxiv - Cancer Biology 2024Quote: ... 1% of PhiX (Illumina) was used as run control ...
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bioRxiv - Genomics 2023Quote: ... All libraries were pooled and subjected to paired-end 75 bp sequencing (paired- end 76 nt reads with the first 1 nt of Read 1 and the last 1 nt of Read 2 trimmed) using the NextSeq500 system (Illumina, CA, USA). For each library ...
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bioRxiv - Microbiology 2023Quote: ... 1 μl of forward and 1 μl of reverse 25 μM PCR primers (Illumina), and 0.5 μl of Phusion high-fidelity DNA polymerase (New England Biolabs) ...
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bioRxiv - Neuroscience 2023Quote: ... collected with the magnet and resuspended in 24ul of Tagmentation Mix (1:1 Illumina 2× Tagmentation buffer ...
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bioRxiv - Immunology 2021Quote: ... Group 1 (The Netherlands, Illumina), Group 2 (France ...
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bioRxiv - Microbiology 2021Quote: ... 1×75 cycles (Illumina, USA). Image analysis was performed in real time by the NextSeq Control Software and Real Time Analysis running on the instrument computer ...
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bioRxiv - Systems Biology 2022Quote: ... 1% PhyX spike-in (Illumina) was added then pooled ...
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bioRxiv - Immunology 2024Quote: ... 1 µL of TDE1 (Illumina), 0.25 µL of 5% digitonin (Promega) ...