Labshake search
Citations for Illumina :
1401 - 1450 of 1525 citations for Recombinant Human Pappalysin 2 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2023Quote: ... 2 μl of the plasmid was used as a template and amplified using the Nextera DNA Indexes (Illumina Inc., San Diego, CA, USA) index primers ...
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bioRxiv - Genetics 2023Quote: ... 2 μg of RNA was used as starting material for library construction with TruSeq RNA Sample Prep Kit V2 (Illumina, RS-122-2002) following the manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: ... 16S rRNA gene amplicon libraries targeting the V1-V3 or V4 region were constructed using a dual-indexing method and sequenced on a MiSeq with a 2×300 bp run format (Illumina, San Diego, CA). Reagent-only negative controls were carried through the DNA extraction and sequencing process.
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bioRxiv - Cancer Biology 2023Quote: ... up to 12 samples were pooled equimolar and paired-end reads 2×150bp sequencing on an Illumina NextSeq 500 instrument using the 300 cycle Mid Output v2.5 Reagent Kit (Illumina, San Diego, CA, USA) according to Illumina guidelines ...
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bioRxiv - Immunology 2023Quote: ... which was performed on a MiSeq instrument with paired-end 2×300 bp reads using the NovaSeq 6000 SP Reagent Kit v1.5 (500 cycles) (Illumina, San Diego, California, USA). The protocol was approved by the ethics committee of Liège University Hospital (approval numbers 2021-54 and 2020/107).
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bioRxiv - Genomics 2023Quote: ... Libraries were quantified and sequenced (paired-end, 2 x 111 cycles) using the Illumina NovaSeq 6000 device (Illumina Inc., San Diego, CA, USA) at IPK Gatersleben54 ...
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bioRxiv - Genomics 2024Quote: ... 11 μL Nuclease-free water and 2 μL RP1 and RPIx primers each (10 mM, for details see Illumina smallRNA indexing primer sequences) to the 10 μL single-stranded DNA product ...
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bioRxiv - Microbiology 2024Quote: ... 2 µl of each P7 and P5 of Nextera XT Index Kit v2 index primers (Illumina Catalogue No. FC-131-2001 to 2004) were added to each well ...
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bioRxiv - Microbiology 2024Quote: ... Metagenome sequencing was performed in two lanes of a NovaSeq S4 flow cell in 2×150bp paired-end format on a NovaSeq 6000 (Illumina, San Diego, USA). Raw sequencing data is available on the NCBI SRA (BioProject PRJNA974462).
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bioRxiv - Evolutionary Biology 2021Quote: ... Subpools were then pooled in equimolar conditions and sequenced at Vienna BioCenter Core Facilities (Vienna, Austria) on Illumina® NextSeqTM 550 (2×150bp, Illumina, San Diego, CA). Sequencing was conducted independently for either bait kit.
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bioRxiv - Molecular Biology 2021Quote: Equimolar ratios of the purified amplicons were pooled and subjected to 2 × 250 bp paired-end sequencing on the MiSeq platform (Illumina, San Diego, CA, USA). To increase the genetic diversity ...
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bioRxiv - Genomics 2020Quote: ... Indexed libraries from multiple patients were pooled and sequenced (2 × 150 cycles) using the MiSeq or the NovaSeq systems (Illumina, San Diego, CA, USA). A step-by-step SARS-CoV-2 target enrichment and sequencing protocol is provided in Appendix I.
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bioRxiv - Genomics 2020Quote: ... according to the instructions for qChIP-Seq and paired-end sequencing (2×75nt) was performed on a NextSeq 500/550 using a HighOutput v2 Kit for 150 cycles (Illumina #FC-404-2002, discontinued).
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bioRxiv - Genomics 2020Quote: ... according to the manufacturer’s instructions and paired-end sequencing (2 × 75 bp) was performed on a NextSeq 500/550 using a HighOutput v2 Kit for 150 cycles (Illumina #FC-404-2002, discontinued).
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bioRxiv - Microbiology 2019Quote: ... denatured using 0.1N NaOH followed by a 2min incubation @96C followed by 5min in an ice-water bath just prior to sequencing as per the Illumina protocol for a 2×301 MiSeq run (Illumina, Inc., San Diego, CA). DNA sequence reads from the Illumina MiSeq were demultiplexed and classified in the following manner ...
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bioRxiv - Microbiology 2020Quote: ... Sequencing was performed at the UW-Madison Biotechnology Center using the 2 × 250 bp paired-end method on an Illumina MiSeq following manufacturer’s guidelines (Illumina, Inc., San Diego, CA, USA). Detailed methods about and the library preparation and sequencing can be found in Skarlupka et al ...
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bioRxiv - Systems Biology 2019Quote: ... Samples were equimolar pooled and sequenced by the University of Wisconsin – Madison Biotechnology Center with the MiSeq 2×250 v2 kit (Illumina, San Diego, CA, USA) using custom sequencing primers.
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bioRxiv - Evolutionary Biology 2021Quote: ... Subsequent equimolar pooling of individual libraries from each plate was performed prior to performing large-scale paired-end sequencing (2 × 146 bp) run on the Illumina NovaSeq sequencing system (Illumina, San Diego, CA, USA). The pipeline used to separate the sequencing output of the individual libraries into FASTQ files of 146bp paired-end reads is available on GitHub at https://github.com/czbiohub/utilities.
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bioRxiv - Evolutionary Biology 2021Quote: ... These samples were sequenced (2 × 150 or paired end 150 nt read length) on the Novaseq 6000 platform (Illumina, Inc, San Diego, CA, USA) by the Roy J ...
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bioRxiv - Evolutionary Biology 2020Quote: ... We sequenced paired-end DNASeq and RNASeq reads (2 x 300) at the University of Iowa using the Illumina MiSeq platform (Illumina Inc., San Diego, CA). After inspecting raw read data ...
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bioRxiv - Microbiology 2020Quote: ... The sample was loaded at a concentration of 10 pM and sequenced using 2×250 paired-end strategy on the Miseq (Illumina San Diego, CA, USA) platform for 251 cycles.
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bioRxiv - Plant Biology 2020Quote: ... Quantification of libraries and sequencing (rapid run, paired-end sequencing, 2 × 100 cycles, on-board clustering) using the Illumina HiSeq2500 device (Illumina, San Diego, California, USA) were as described previously 44.
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bioRxiv - Plant Biology 2021Quote: ... 10 ng of template DNA and index primer 1 (N7xx) and index primer 2 (N5xx) of Nextera XT Index Kit v2 Set A (Illumina, San Diego, CA, USA) according to the manufacturer’s instruction ...
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bioRxiv - Microbiology 2020Quote: ... The resulting sequencing libraries were provided to the Cornell University Biotechnology Resource Center for paired-end Illumina MiSeq sequencing (2 × 250 bp) (Illumina, Inc., San Diego, CA). A 20 pM concentration of phiX spike-in at 10-20 % [v/v] was added to 16S and rpoB gene sequencing libraries to improve the quality of the low diversity amplicon libraries.
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bioRxiv - Microbiology 2021Quote: ... Extracted DNA was sent to the University of Illinois Keck Center for Fluidigm sample preparation and paired-end (2 × 250 nt) sequencing on an Illumina NovaSeq (Illumina, San Diego, CA, USA). The 16S rRNA gene fragment was amplified using the primers V3_F357_N ...
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bioRxiv - Microbiology 2021Quote: ... Libraries were then sequenced on a NovaSeq 6000 instrument with a SP flow cell (2 x 250 bp) (Illumina Inc., San Diego, CA, USA).
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bioRxiv - Microbiology 2019Quote: ... normalised and denatured libraries spiked with 2% PhiX Control Library were sequenced using the CSIRO AAHL MiniSeq Sequencing System (Illumina, San Diego, CA, USA) using the High-Output Reagent kit (300-cycles) ...
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bioRxiv - Cancer Biology 2020Quote: ... The libraries were run on the Illumina Next Seq 500 using the High Output 75 cycles kit (2 x 36 cycles, paired end reads, single index; Illumina, San Diego, CA, USA).
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bioRxiv - Immunology 2022Quote: ... Paired-end (2×75) sequencing was performed to an average of 40 million reads per sample on a HiSeq 3000 (Illumina, San Diego, CA, USA).
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bioRxiv - Molecular Biology 2022Quote: ... a final dilution to 4nM was performed and 13 pM of the final denaturated library was loaded onto the Illumina MiSeq® with 10% PhiX using 2 × 300 bp v3 chemistry (Illumina Inc., CA, USA).
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bioRxiv - Cancer Biology 2020Quote: ... The tagmented and multiplexed cDNA libraries were sequenced in Nextseq 500 using Nextseq 500 High Output v.2 kit (75 cycles, Illumina Cat# FC-404-2005) following Macosko et al ...
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bioRxiv - Microbiology 2020Quote: ... Next Generation Sequencing (NGS) was performed on an Illumina MiniSeq® using a Mid Output Kit (2×150-cycles) (Illumina, San Diego, CA, USA) by loading 0.8 pM on the flowcell ...
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bioRxiv - Microbiology 2022Quote: Purified amplicons were pooled in equimolar quantities and paired-end sequenced (2 × 300) according to standard protocols on an Illumina MiSeq platform (Illumina, San Diego, CA, USA) by Majorbio Bio-Pharm Technology Co ...
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bioRxiv - Genomics 2022Quote: ... Barcoded libraries were pooled in equimolar amounts and sequenced as 2×300mer reads on an Illumina MiSeq platform using a MiSeq Reagent Kit v3 (Illumina, Cat#: MS-102-3003). 50% PhiX Control v3 (Illumina ...
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bioRxiv - Microbiology 2019Quote: ... a 20 pM pooled library was subjected to paired-end (2×250 bp) high-throughput sequencing on an Illumina MiSeq platform (Illumina, San Diego, CA, USA) using MiSeq reagent kit v2 ...
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bioRxiv - Physiology 2020Quote: ... RNA-seq libraries were sequenced using paired-end reads (50-nt read 1 and 25-nt read 2) on a NextSeq 500 instrument (Illumina, San Diego, CA, USA). Obtained reads were mapped to the mouse GRCm38.p6 genome using STAR (version 2.7.3a) ...
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bioRxiv - Immunology 2019Quote: ... All samples were subjected to an indexed PE sequencing run of 2×51 cycles on an Illumina HiSeq 2000 (Illumina, San Diego, CA, USA) (3-4 samples/lane) ...
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bioRxiv - Immunology 2019Quote: ... The V4 variable region of the 16S rRNA gene was amplified using universal bacterial primers(Klindworth et al., 2013) which were uniquely barcoded for each sample (Illumina Nextera Indexes Version 2). The amplicons were purified ...
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bioRxiv - Microbiology 2019Quote: ... All samples were subjected to an indexed paired-end sequencing run of 2×51 cycles on an Illumina HiSeq 2000 system (Illumina, San Diego, CA, USA) (16 samples/lane) ...
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bioRxiv - Pathology 2020Quote: The V3-V4 hypervariable regions of the 16S_rDNA were amplified by two steps PCR using v1 primers (Vaiomer) and sequenced using MiSeq Reagent Kit v3 (2×300 bp Paired-End Reads, Illumina, San Diego, CA, USA) as previously described [24] ...
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bioRxiv - Microbiology 2021Quote: Paired-end libraries were constructed with 0.2 ng/μl of purified DNA using the Nextera XT DNA Library Prep Kit as recommended by the manufacturer (Illumina, San Diego, CA, USA) and were quantified using Qubit fluorometer (ThermoFisher Scientific ...
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bioRxiv - Microbiology 2021Quote: ... following the manufacturer’s instructions and Whole Genome Sequencing was performed using Illumina Miseq platform with a 2 x 250 paired-end run after Nextera XT library preparation (Illumina Inc., San Diego, CA).
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bioRxiv - Microbiology 2021Quote: ... Library preparations and sequencing were performed at the Research Resource Centre at the University of Illinois with pair end (2 x 300 bp) MiSeq platform (Illumina, San Diego, CA, USA). Due to low concentrations of ribosomes in the dry soil collected during the summer of 2017 ...
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bioRxiv - Microbiology 2021Quote: ... High-throughput amplicon sequencing covering the V4-V5 region of the 16S rRNA gene was performed on an Illumina Miseq 2 × 300 paired-end platform (Illumina, San Diego, CA USA) using the universal primers 515F (GTGYCAGCMGCCGCGGTAA ...
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bioRxiv - Evolutionary Biology 2020Quote: ... we used 2 μg total RNA for library preparation following the protocol in the TruSeq Stranded mRNA Library Prep Kit (Illumina, San Diego, CA, USA). The samples were sequenced by single-end reads (75 bp ...
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bioRxiv - Genetics 2021Quote: ... The thirty-seven sequencing libraries were created by reverse-transcription from 2 µg of RNA from each sample using the TruSeq Stranded mRNA Library Prep kit (Illumina, San Diego, CA, USA). Index adapters were added to identify sequences for each sample in the final data ...
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bioRxiv - Immunology 2020Quote: ... All 11 samples were subjected to an indexed paired-end sequencing run of 2×100bp on an Illumina Novaseq 6000 system (Illumina, San Diego, CA, USA).
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bioRxiv - Molecular Biology 2019Quote: ... the libraries were sequenced on a single MiSeq run using an Illumina MiSeq Reagent Kit v2 for 2 × 150 bp PE (Illumina, San Diego, CA, USA). All sequencing reads obtained in the present study were deposited in the DNA Data Bank of Japan (DDBJ ...
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bioRxiv - Genomics 2021Quote: ... and four mate-pair sequencing libraries (insert sizes of 2, 5, 10, and 15 kb) in accordance with manufacturer protocols (Illumina, San Diego, CA, USA). Libraries were then sequenced using a HiSeq2000 instrument (Illumina) ...
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bioRxiv - Genomics 2021Quote: ... and 2 × 150 bp reads were produced on an Illumina NovaSeq 6000 instrument using the NovaSeq S2 reagent kit (Illumina, San Diego, California, USA). An average of 57,521,215 reads were generated for each of the 4 samples ...