Labshake search
Citations for Illumina :
1001 - 1050 of 1525 citations for Recombinant Human Pappalysin 2 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genomics 2020Quote: ... Poly-A-enriched RNA was used to prepare libraries with TruSeq RNA Sample Preparation kit v2 y v4 (ref. RS-122-2001/2, Illumina) according to instructions from manufacturer followed by single-end (run1 ...
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bioRxiv - Developmental Biology 2021Quote: ... Long mRNA libraries (2 replicates per time-point) were built using the TruSeq Stranded mRNA HT Sample Prep Kit (Illumina). Library concentration was assessed for all libraries using the Qubit fluorimetric system (Invitrogen ...
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bioRxiv - Evolutionary Biology 2020Quote: ... Libraries were pooled in equimolar ratios and sequenced in a single lane of 2 x150 paired-end sequencing on an Illumina NovaSeq 6000 (Illumina).
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bioRxiv - Evolutionary Biology 2021Quote: ... Samples were sequenced on an Illumina HiSeq 4000 producing 2×150 bp paired-end reads (Illumina, San Diego, CA, USA) at Novogene (Sacramento ...
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bioRxiv - Immunology 2019Quote: VH and VL libraries from sorted B cell were subjected to NGS on the MiSeq platform with the reagent kit V3 2×300 bp paired-end (Illumina), using an input concentration of 16pM with 5% PhiX.
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bioRxiv - Genomics 2020Quote: ... Paired-end read sequencing (2×150 bp) was performed on the Illumina HiSeq X (Illumina, Inc., San Diego, CA, USA) using the Chromium library.
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DNA and RNA-SIP reveal Nitrospira spp. as key drivers of nitrification in groundwater-fed biofiltersbioRxiv - Physiology 2019Quote: ... paired-end 16S rRNA amplicon sequencing was done on the Illumina MiSeq platform with MiSeq Reagent Kit v3 (2 × 301 bp; Illumina). All sequencing was performed at the National High-throughput DNA Sequencing Center (Copenhagen ...
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bioRxiv - Evolutionary Biology 2019Quote: ... using the Sequence by Synthesis technique (providing 2×125 bp reads, respectively) with base calling achieved by the RTA software (Illumina). The Pool-Seq data were deposited in the Sequence Read Archive (SRA ...
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bioRxiv - Microbiology 2019Quote: ... and was sequenced from paired ends using 500 cycles with the HiSeq 2500 (2 x 250 bp) system (Illumina, USA).
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bioRxiv - Genomics 2019Quote: ... trachomatis enriched libraries were subjected to cluster generation and paired-end sequencing (2 × 150 bp) in an Illumina MiSeq equipment (Illumina), according to the manufacturer’s instructions.
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bioRxiv - Microbiology 2019Quote: ... Pooled libraries were sequenced with paired-end reads (2×150-250) on a MiSeq system using v2 or v3 sequencing reaction chemistry (Illumina). Data were analyzed by CLC genomics workbench v12 software (Qiagen) ...
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bioRxiv - Pathology 2019Quote: ... Two hundred picomolar pooled libraries were utilized per flowcell for clustering amplification on cBot using HiSeq 3000/4000 PE Cluster Kit and sequenced with 2×75bp paired- end configuration on HiSeq4000 (Illumina) using HiSeq 3000/4000 PE SBS Kit ...
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bioRxiv - Genomics 2019Quote: ... We normalized in 2 different quantities: 130 pg and 750 pg were used as input for the Nextera XT kit (Illumina). The Nextera technology uses a modified tranposase to fragment and ligate adapters in a unique step called “tagmentation” ...
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bioRxiv - Microbiology 2019Quote: ... The resulting amplicon library pool was diluted to 2 nM with sodium hydroxide and 5 mL were transferred into 995mL HT1 (Illumina) to give a final concentration of 10 pM ...
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bioRxiv - Neuroscience 2019Quote: ... We pooled and sequenced the libraries with paired-end reads (50 bases for read 1 and 25 bases for read 2) on two flowcells with a NextSeq 500 instrument (Illumina).
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bioRxiv - Plant Biology 2019Quote: ... The sequencing was performed with 2 x 100 bp read length of the flow cell loaded on a HiSeq 2500 sequencing system (Illumina).
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bioRxiv - Genomics 2019Quote: ... and three mate-pair libraries (insert sizes of 2 kb, 5 kb, and 8 kb) were constructed using the TruSeq PCR-free Kit (Illumina) and Mate-pair Kit (Illumina) ...
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bioRxiv - Cancer Biology 2019Quote: ... The RNA-seq library was prepared using 2 µg of total RNA with a TruSeq sample prep kit v2 (Illumina) according to the manufacture’s protocol and analyzed by single-end sequencing using NextSeq (Illumina).
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bioRxiv - Genetics 2019Quote: ... Patients with a diagnosis of FECD and scheduled for endothelial keratoplasty between the dates of 2/12/2013 and 10/27/2014 (for Illumina Infinium HumanMethylation450 BeadChip analysis ...
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bioRxiv - Microbiology 2019Quote: ... Paired-end sequencing (2 × 150 bp) was performed on a 300 bp PCR-free insert library on a HISeq 2500 (Illumina) at Novogene (HK ...
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bioRxiv - Microbiology 2019Quote: ... The indexed libraries were pooled and diluted to 1.5pM for paired end sequencing (2× 81 cycles) on a NextSeq 500 instrument using the v2 150 cycle High Output kit (Illumina) as per manufacturer’s instructions (generating 80bp paired-ends) ...
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bioRxiv - Bioengineering 2020Quote: ... The barcoded amplicon samples were then sent to the UCLA Technology Center for Genomics & Bioinformatics for multiplex sequencing with 2 x 300 paired-end configuration in a single-lane flow cell of MiSeq instrument (Illumina). Fastq paired-end raw data were filtered ...
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bioRxiv - Genomics 2021Quote: ... Paired-end sequencing was completed using NextSeq 500/550 300-cycle High-Output Kits v 2.5 (Illumina; 2 × 150 cycles). High-Output kit used for the generation of the NextSeq data allows for over 25 times more sequence data to be generated compared to MiSeq v2 sequencing (800M versus 30M paired reads ...
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bioRxiv - Systems Biology 2020Quote: ... then performed paired-end sequencing of these libraries (38 cycles read 1 + 37 cycles read 2) on a NextSeq 500 (Illumina). After sequencing ...
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bioRxiv - Genomics 2020Quote: PDT and ATAC libraries were pooled and paired-end sequenced (2 x 34 cycles) using Nextseq High Output Cartridge kits on a Nextseq 550 machine (Illumina). Raw sequencing data were demultiplexed with CellRanger-ATAC mkfastq ...
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bioRxiv - Genomics 2021Quote: ... ChIP-seq libraries were prepared using the ThruPLEX DNA-seq Kit (Rubicon) and sequenced paired-end 2 x 100bp on the HiSeq 2500 (Illumina).
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bioRxiv - Microbiology 2021Quote: ... Libraries were sequenced using 2 x 150 nt paired-end sequencing runs (4 lanes on separate runs) on NextSeq Genome Sequencer (Illumina) with a NextSeq 500/550 High Output Kit v2.5 at Core Facility for Scientific Research – University of Sao Paulo (CEFAP-USP).
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bioRxiv - Cancer Biology 2021Quote: ... 10 cycles per index and 120 cycles for read 2 on a NextSeq 500/550 High Output Kit v2.5 (Illumina, 20024907).
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bioRxiv - Systems Biology 2021Quote: ... NGS was performed on a final adjusted library pool using the paired-end 300-cycle NovaSeq 6000 S2 XP Reagent Kit (2 × 150 bp) on a NovaSeq instrument (both Illumina) per the manufacturer’s instructions ...
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bioRxiv - Systems Biology 2021Quote: ... Concentration of each sample in the pooled libraries were determined using the paired-end 300-cycle MiSeq Reagent Nano Kit v2 (2 × 150 bp) on a MiSeq instrument (both Illumina). NGS was performed on a final adjusted library pool using the paired-end 300-cycle NovaSeq 6000 S2 XP Reagent Kit (2 × 150 bp ...
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bioRxiv - Cell Biology 2021Quote: ... was performed by incubating beads with tagmentation buffer (12.5 µl 2 x TD buffer, 11.5 µl nuclease free water, 1µl Tn5 enzyme (Illumina #FC-131-1024)) at 37°C for 10min ...
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bioRxiv - Cell Biology 2021Quote: ... Libraries were prepared by Poly A selection from total RNA and sequenced using HiSeq 2 x 150 bp sequencing (Illumina). FASTQ files from Genewiz were used for further analysis analyses were performed using the statistical computing environment R (v4.0.0) ...
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bioRxiv - Cell Biology 2021Quote: ... 8 bases for index 1 and 8 bases for index 2) using the NextSeq 500 High Output Kit 75-cycles (#FC-404-1005, Illumina) loaded at 1.8pM and including 1 % PhiX ...
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bioRxiv - Microbiology 2020Quote: ... The libraries were sequenced on the MiSeq™ platform using the MiSeq™ Reagent Kit v3 (2 × 250 bp; Illumina).
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bioRxiv - Systems Biology 2021Quote: ... and 2 × 100 base paired-end sequencing libraries were sequenced using the Illumina HiSeq 2500 with v3 sequencing reagents (Illumina).
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bioRxiv - Plant Biology 2021Quote: ... The final library was diluted to a loading concentration of 1.4 pM for cluster generation and 2 × 150 bp paired-end sequencing on the Illumina NextSeq500 system (Illumina Inc) alongside a 5% PhiX spike-in control library ...
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bioRxiv - Microbiology 2020Quote: ... Read 1 and 2 adapter recognition sequences were provided for adapter removal (Illumina TruSeq Adapter Read 1: AGATCGGAAGAGCACACGTCTGAACTCCAGTCACNNNNNNATCTCGTATGCCGTCTTCTGCT TG, Illumina TruSeq Adapter Read 2 ...
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bioRxiv - Microbiology 2020Quote: ... The PCR product mixture was sent to FASMAC to acquire 2 × 250 bp paired-end sequences using the MiSeq platform (Illumina).
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bioRxiv - Genomics 2021Quote: ... quantified by Qubit and analyzed for fragment distribution with a Bioanalyzer and used for paired-end sequencing (2 x 75 cycles) on a MiSeq System (Illumina).
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bioRxiv - Genomics 2021Quote: ... analyzed for fragment distribution with a Bioanalyzer and sequenced with paired-end settings (2 x 75 cycles) on a MiSeq System (Illumina).
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bioRxiv - Microbiology 2020Quote: ... At Gdansk two independent protocols were used for SARS-CoV-2 genome sequencing: Illumina RNA prep with enrichment for respiratory virus oligos panel V2 followed by Illumina MiniSeq medium output run that produced 150-nucleotide paired-end reads ...
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bioRxiv - Microbiology 2021Quote: ... The purified PCR products were then processed and sequenced using the NextSeq 75 – High Output (82 cycles in read 1, 8 cycles in index 1, and 8 cycles in index 2 SE reads) (Illumina). The sequencing data was analyzed using the Model-Based Analysis of Genome-wide CRISPR/Cas9 Knockout (MAGeCK ...
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bioRxiv - Microbiology 2021Quote: ... Sequencing was performed in paired-end mode with a SP flow cell (2 x 50 cycles) using NovaSeq 6000 sequencer (Illumina).
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bioRxiv - Microbiology 2021Quote: ... Illumina sequencing (2× 300 bp paired-end reads) was conducted using an Illumina MiSeq platform (Illumina, San Diego, California, USA) at JAMSTEC ...
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bioRxiv - Microbiology 2020Quote: ... Multiplexed paired-end libraries (2 × 150 bp) were prepared using the Nextera XT DNA kit (Illumina, San Diego, CA, USA) and eventually sequenced with an Illumina NextSeq-500 instrument at a minimum of 50X coverage depth ...
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bioRxiv - Cancer Biology 2020Quote: ... Parental and prostate tumor-passaged chromosomal genomic DNA were sequenced following the standard Next Generation Sequencing NovaSeq 2×100 protocol (Illumina) and aligned against the parental LT2 and associated stable pSLT reference sequences (NCBI ...
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bioRxiv - Cell Biology 2021Quote: ... The final libraries were generated through size selection and PCR enrichment and sequenced as 2×150bp on a HiSeq2500 Sequencer (Illumina). Samples were sequenced to an approximate depth of 35–40 million reads per sample ...
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Mitochondrial mutations in Caenorhabditis elegans show signatures of oxidative damage and an AT-biasbioRxiv - Genetics 2021Quote: ... 2) calls duplex consensus sequences (DCSs) based on shared random barcodes (each DCS required a minimum of six raw Illumina reads – at least three from each strand) ...
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bioRxiv - Microbiology 2021Quote: ... Libraries were purified using Ampure XP beads and sequenced using MiSeq V2 (150bp x 2) chemistry (Illumina, San Diego, CA). 18S V4+V5 amplification was achieved using the iProof DNA polymerase (Bio-Rad Laboratories ...
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bioRxiv - Molecular Biology 2022Quote: ... followed by library construction using non-stranded (Replicate 1) or stranded (Replicates 2 and 3) TruSeq mRNA Library Prep Kit (Illumina). The resulting libraries were sequenced on Hiseq4000.