Labshake search
Citations for Illumina :
1251 - 1300 of 9681 citations for ssc mir 299 Real time RT PCR Detection Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2019Quote: PCR amplification products of the V5–V7 region of fungal 18S and bacterial 16S rRNA genes were sequenced by Illumina MiSeq sequencer ...
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bioRxiv - Genomics 2019Quote: ... Each sample library was uniquely barcoded and quantified by PCR using a PhiX Control v3 (Illumina, Cat #FC-110-3001) standard curve ...
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bioRxiv - Microbiology 2019Quote: ... universal bacterial 16S rRNA genes were PCR amplified using 926F and 1392wR primers and then sequenced on an Illumina MiSeq using 30% PhiX Control v3 (Illumina) and a MiSeq Reagent Kit v3 (600 cycles ...
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bioRxiv - Synthetic Biology 2020Quote: ... Adaptors were added to these PCR products and then the pooled libraries were subjected to 300 cycle single-end sequencing using MiSEQ (Illumina). Data was analyzed using Geneious 9.0.5 NGS analysis tools.
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bioRxiv - Microbiology 2019Quote: ... Paired-end sequencing (2 × 150 bp) was performed on a 300 bp PCR-free insert library on a HISeq 2500 (Illumina) at Novogene (HK ...
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bioRxiv - Genomics 2021Quote: ... Following immunoprecipitation with Dynabeads Protein G beads (invitrogen) in PCR tubes, samples were subject to Tagmentation (5 µl Tagmentation Buffer, 1 µl Tagmentation DNA Enzyme (Illumina), 19 µl Nuclease free water ...
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bioRxiv - Cancer Biology 2020Quote: ... The sgRNA sequences were recovered by genomic PCR analysis and deep sequencing using MiSeq for single-end 150-bp read length (Illumina). The primer sequences used for cloning and sequencing are listed in Supplementary Table S1 ...
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bioRxiv - Microbiology 2020Quote: ... The PCR product mixture was sent to FASMAC to acquire 2 × 250 bp paired-end sequences using the MiSeq platform (Illumina).
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bioRxiv - Microbiology 2021Quote: ... High-throughput pyrosequencing of the PCR products was performed using the Illumina MiSeq platform (Illumina, Inc., San Diego, CA, USA). Paired-end reads of the original DNA fragments were excluded from the analysis if they did not well-match a 12-base Golay barcode (one or no errors) ...
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bioRxiv - Cell Biology 2021Quote: ... The final libraries were generated through size selection and PCR enrichment and sequenced as 2×150bp on a HiSeq2500 Sequencer (Illumina). Samples were sequenced to an approximate depth of 35–40 million reads per sample ...
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bioRxiv - Biophysics 2022Quote: ... The extracted DNA was then amplified by PCR and attached to barcode oligonucleotides compatible with the MiSeq sequencing platform (Illumina, index1 ...
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bioRxiv - Microbiology 2022Quote: ... The PCR-generated amplicon libraries were subjected to paired-end 2×250 Illumina MiSeq sequencing (Illumina, San Diego, CA, USA).
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bioRxiv - Cancer Biology 2022Quote: ... and amplified for 12 cycles using the SI-PCR forward primer (10x Genomics) and a Nextera i7 reverse primer (Illumina). For the HTO-containing fraction ...
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bioRxiv - Cancer Biology 2022Quote: ... the ADT fraction was amplified for 10 cycles with SI-PCR oligo (10x Genomics) and TruSeq Small RNA RPI-x (Illumina) primers to index the samples ...
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bioRxiv - Evolutionary Biology 2021Quote: We prepared the Illumina sequencing libraries by performing two consecutive rounds of PCR following the approach of Fluidigm System (Access ArrayTM System for Illumina Sequencing Systems ...
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bioRxiv - Cancer Biology 2020Quote: ... Single cell barcoded cDNA libraries were quantified by quantitative PCR (Kappa Biosystems) and sequenced on an Illumina NextSeq 500 (Illumina). Read lengths were 26 bp for read 1 ...
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bioRxiv - Cancer Biology 2021Quote: ... The sequencing library was generated by PCR and used to produce the clusters thereafter sequenced on NovaSeq 6000 System (Illumina). Each sample was sequenced in a separate flow cell lane ...
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bioRxiv - Developmental Biology 2022Quote: ... Extracted DNA was PCR-amplified (F 5’ – GTGCCTTCTCCGTCAGTCTC – 3’, R 5’ – GCAGGCACAAATCCAAGTTT – 3’, and subsequently subjected to next-generation sequencing in an Illumina MiSeq platform 116 ...
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bioRxiv - Cancer Biology 2022Quote: ... resistant and P12 cells of cell lines #3 and #9 was performed at the Genomics and Proteomics Core Facility of the German Cancer Research Center (GPCF DKFZ, Heidelberg) using the TruSeq DNA PCR-free Methyl protocol (Illumina) for library preparation ...
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bioRxiv - Microbiology 2019Quote: ... the 16S rRNA sequences covering the V6-V7-V8 variable regions (5’ ACACTGACGACATGGTTCTACA 3’ and 5’ TACGGTAGCAGAGACTTGGTCT 3’) were PCR amplified and sequenced by Illumina MiSeq PE250 (paired-end) ...
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bioRxiv - Microbiology 2019Quote: The 2nd round barcoded PCR was performed by using sixteen forward and twenty-four reverse barcoded primers obtained from Illumina MiSeq protocols (S2 Table) ...
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bioRxiv - Microbiology 2019Quote: ... using a modified PowerWater® Sterivex™ DNA Kit and from cryoconite using a PowerBiofilm™ RNA Isolation Kit (MO BIO Laboratories) prior to 16S rRNA gene and 16S rRNA (cDNA) quantitative PCR (qPCR) and V3-V4 region MiSeq (Illumina) sequencing ...
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bioRxiv - Cell Biology 2020Quote: To the cancer hot spot library P5 and P7 sequences were attached by PCR using the custom pBB9 primer and Nextera N701 (Illumina). Library was purified with 0.6x volume fraction AMPure beads ...
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bioRxiv - Microbiology 2020Quote: ... colonies were tested for their sensitivity to streptomycin and mutants were confirmed by PCR across the deleted region and further verified by Illumina sequencing.
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bioRxiv - Genomics 2020Quote: ... Analysis of the screen is as follows: the sequences flanking retroviral insertion sites were determined using inverse PCR followed by Illumina sequencing ...
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bioRxiv - Biochemistry 2019Quote: ... Libraries for Illumina Next Generation Sequencing (NGS) were produced with the two-step PCR approach pooled in appropriate ratios and analyzed by Illumina NGS.
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bioRxiv - Bioengineering 2019Quote: ... For library amplification 12 PCR cycles were applied and 45-48 libraries were pooled for sequencing on HiSeq 2000 or HiSeq 2500 instruments (Illumina) using 50 base pair single end reads.
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bioRxiv - Genetics 2020Quote: Nextera barcode adapters were added to can1 amplicons and were then minimally PCR amplified (8 cycles) for attachment of Illumina Nextera XT index primers set A (Illumina). After PCR ...
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bioRxiv - Molecular Biology 2021Quote: ... The sgRNA composition of the population from each replicate was PCR amplified and barcoded followed by next-generation sequencing on a MiSeq (Illumina) using 150-cycle ...
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bioRxiv - Developmental Biology 2021Quote: ... 25 mL of 2x NEBNext Ultra II Q5 Master Mix, 3 mL of 10 mM Universal PCR primer from Illumina, 3 mL of 10mM Index PCR primer and 9 mL of nuclease-free water and amplified for 10 cycles (98C for 10 s ...
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bioRxiv - Microbiology 2020Quote: ... Samples were processed for WGS using multi-segment PCR [10] and then prepared for deep sequencing using the Nextera XT library preparation reagent (Illumina). Samples were sequenced on the Illumina NextSeq platform ...
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bioRxiv - Genomics 2021Quote: Final samples for both inverse PCR and UMI-amplicons (concentration 4 nM) were sequenced as paired-end reads on HiSeq2500 and NextSeq500 sequencers (Illumina).
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bioRxiv - Genomics 2022Quote: ... The DNA fragments cut by the enzyme were amplified by PCR and then sequenced with 2X150 bp chemistry on the Illumina HiSeq X Ten platform (Illumina).
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bioRxiv - Microbiology 2022Quote: Both the PCR products from all the four cohorts were bead purified and subjected to another round of PCR with dual indices and adapters to generate the respective libraries as recommended by Illumina. The cleaned libraries were quantitated on Qubit® flurometer and appropriate dilutions loaded on a D1000 screen tape to determine the size range of the fragments and the average library size ...
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bioRxiv - Neuroscience 2022Quote: ... DNA was then subjected to PCR to target the 16S V3-V4 region and to generate the libraries as recommended by Illumina MiSeq sequencing (Illumina ...
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bioRxiv - Physiology 2022Quote: ... Multiplexing was conducted by ligating an 8-base molecular barcode to sequences before 15 cycles of PCR amplification and HiSeq 2500 (Illumina) Rapid Mode library sequencing ...
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bioRxiv - Molecular Biology 2022Quote: ... The PCR product was used to construct libraries and sequenced on an Illumina NovaSeq 6,000 (Illumina, San Diego, CA, USA) with 150 bp paired-end reads ...
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bioRxiv - Biochemistry 2024Quote: ... Illumina sequencing adaptors were introduced by PCR and the final library sequenced at the JHU Genetic Resources Core Facility on a HiSeq2500 (Illumina) in Rapid Mode using 50 bp single reads ...
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bioRxiv - Molecular Biology 2024Quote: ... Illumina indexes were added by amplifying 1 ng DNA to a 50 μl PCR reaction using Kapa HS enzyme and unique dual indexing primers (Illumina) for 11 cycles (Denaturation 95°C 3min ...
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bioRxiv - Developmental Biology 2024Quote: ... Another cleanup step with SPRIselect was performed before the Sample Index PCR when P5 and P7 sequences (for Illumina sequencing) and sample indices (to allow for multiplexing in the sequencer ...
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bioRxiv - Microbiology 2024Quote: ... PCR products were checked by gel electrophoresis and sequencing was performed using a MiSeq sequencer (Illumina Inc., San Diego, CA) using a 600 bp kit ...
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bioRxiv - Microbiology 2022Quote: ... Gel-extracted DNA (size selection ~250 to 300bp) was amplified by PCR using primers and cycling conditions recommended by Illumina. Purified PCR products were submitted to the UW-Madison Biotechnology Center for a single end 50-bp Illumina MiSeq run ...
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bioRxiv - Physiology 2023Quote: ... The subsequent steps of library preparation were carried out on the Agilent NGS Bravo workstation in 96-well plates adapted for the TruSeq PCR-free Sample Preparation protocol by Illumina.
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bioRxiv - Genetics 2023Quote: ... Locus specific PCR product was used for indexing PCR using KOD Hot Start DNA Polymerase and TruSeq i5 and i7 indexing primers (Illumina) following the cycling conditions ...
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bioRxiv - Immunology 2023Quote: ... The simultaneous antibody binding leads to hybridization of unique DNA oligonucleotides that serve as templates for polymerase-dependent extension (DNA barcoding) followed by PCR amplification and NovaSeq (Illumina) DNA sequencing as published 90 ...
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bioRxiv - Genetics 2023Quote: ... Purified PCR products were prepared for sequencing using the standard Illumina DNA Prep protocol and reagents (Illumina DNA Prep (M), IPB) ...
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bioRxiv - Developmental Biology 2023Quote: ... genotyped by PCR (genotyping primer sequences are presented in Table S4) and confirmed by Sanger Sequencing (Quintara Bio) or MiSeq (Illumina). Southern Blot using a CITRINE probe was carried out to verify single integration for the reporter cell lines.
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bioRxiv - Cell Biology 2023Quote: ... The resulting cDNA was amplified with 25 cycles of PCR and libraries will be prepared for sequencing with standard NexteraXT Illumina protocol (Illumina).
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bioRxiv - Microbiology 2023Quote: ... Libraries for high-throughput sequencing of PCR products were prepared following the “16S Metagenomic Sequencing Library Preparation” protocol by Illumina, using primers with extensions containing the adapters and the MiSeq Reagent Kit v2 (CaBM_D219A_Fw and CaBM_D219A_Rv ...
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bioRxiv - Immunology 2023Quote: ... The V3-V4 region of 16s rDNA was amplified by PCR and sequenced in the MiSeq platform at THSTI (Illumina) using the 2 × 250 bp paired-end protocol yielding pair-end reads that overlap almost completely ...