Labshake search
Citations for Illumina :
1001 - 1050 of 1329 citations for Recombinant Human CD19 protein Fc tagged R PE labeled since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2022Quote: ... Purified libraries were sequenced on Illumina MiSeq platform (reagent kits: v2 300-cycles, paired-end mode) at 8 pM loading concentration with 25% PhiX spike-in (Illumina FC-110-3001). Custom sequencing primers were spiked into reagent cartridge (well 12 ...
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bioRxiv - Neuroscience 2023Quote: ... Multiplex samples Pool (1.65 pM including PhiX 1%) was loaded in NextSeq 500/550 High Output v2 kit (75 cycles) cartridge (catalog #FC-404-1005; Illumina, San Diego, CA) and loaded on NextSeq 500 System machine (Illumina) ...
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bioRxiv - Genomics 2022Quote: ... spun at 300G for 12 min at 4 ºC and resuspended in 20 uL transposition mix: 1X TD buffer and 2 uL of TDE1 (Illumina #FC-121-1030), 0.01% Digitonin in DMSO ...
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bioRxiv - Molecular Biology 2022Quote: ... before final pooling and subsequent run on a MiniSeq Sequencing System using the Miniseq High Output Kit (75 cycles) (Illumina #FC-420-1001) with a 10% of phiX spike-in.
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bioRxiv - Biophysics 2023Quote: ... The DNA concentration was measured using the Qubit dsDNA BR assay kit and sequenced using a HiSeq SBS v4 50 cycle kit (Illumina FC-401-4002) and HiSeq SR Cluster Kit v4 (Illumina GD-401-4001 ...
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bioRxiv - Immunology 2023Quote: ... 100,000 nuclei per sample were isolated using an iodixanol gradient and ATAC-seq was subsequently performed according to the original protocol (Buenrostro et al, 2013) using Nextera DNA Sample preparation kit (Illumina, FC-121-1030). After library amplification ...
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bioRxiv - Genomics 2023Quote: ... The filtered nuclei were pelleted at 500 r.c.f for 5 min in a pre-chilled fixed-angle centrifuge and then resuspended in 25 μl of 1.25x Tagment DNA Buffer (Nextera XT Kit, Illumina Inc. FC-131-1024). For cell cultures ...
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bioRxiv - Cancer Biology 2023Quote: ... of libraries were pooled and subjected to sequencing of approximately 20 million reads per sample using the Mid Output v2 kit (Illumina, FC-404-2001) on a Illumina NextSeq550 following the manufacturer’s instructions.
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bioRxiv - Genomics 2023Quote: ... about 1.5 ng purified pre-amplified cDNA sample was fragmented in four 20 μl tagmentation mix (10 μl 2x TD buffer, 5 μl Nextera XT (Illumina, FC-131-1096), and 5 μl cDNA sample ...
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bioRxiv - Genetics 2023Quote: ... Pooled libraries were diluted and processed for 35-bp paired-end sequencing on an Illumina NextSeq 500 instrument (ID: NB501524) using the NextSeq 500 High Output v2 kit (75 cycles) (Illumina; FC-404-2005) in accordance with the manufacturer’s instructions.
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bioRxiv - Genomics 2023Quote: ... The pelleted nuclei were gently resuspended with 50 µl of transposition reaction containing 25 µL 2X Tagmentation buffer (Illumina Cat FC-121-1030), 5 µL Tn5 transposase (Illumina Cat FC-121-1030 ...
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bioRxiv - Genetics 2023Quote: ... Pooled libraries were diluted and processed for either 75-bp single-end or 35-bp paired-end sequencing on an Illumina NextSeq 500 instrument (ID: NB501524) using the NextSeq 500 High Output v2 kit (75 cycles) (Illumina; FC-404-2005) in accordance with the manufacturer’s instructions.
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bioRxiv - Developmental Biology 2023Quote: ... 600 pg of pre-amplified cDNA were then used as input for Tn5 transposon tagmentation by the Nextera XT DNA Library Preparation Kit (Illumina, FC-131-1096) followed by 12 cycles of library amplification ...
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bioRxiv - Biochemistry 2023Quote: ... The DNA concentration was measured using the Qubit dsDNA BR assay kit and sequenced using a HiSeq SBS v4 50 cycle kit (Illumina FC-401-4002) and HiSeq SR Cluster Kit v4 (Illumina GD-401-4001 ...
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bioRxiv - Genomics 2023Quote: ... The tagmentation and library preparation was performed with the Nextera DNA Library Preparation Kit with 8 cycles of PCR amplification (Illumina #FC-121-1030).
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bioRxiv - Genomics 2023Quote: ... 4 μl of 1 ng amplicon DNA was combined with a mix containing 1 μl of Amplicon Tagment Mix (Illumina, FC- 131-1096) and 5 μl of Tagment DNA Buffer (Illumina ...
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bioRxiv - Developmental Biology 2023Quote: ... Libraries were generated from the resulting cDNA (0.2ng/ul per sample) using the Nextera XT DNA library preparation kit (Illumina, Cat.no. FC-131-1024) as previously described68 ...
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bioRxiv - Cancer Biology 2023Quote: ... The cell pellet was resuspended in Illumina TD buffer and 2.5 µl of Tn5 enzyme was added to the transposition reaction (Illumina, Cat # FC-121-1030). Nuclei were incubated at 37°C for 60 minutes and fragmented DNA was purified using the MinElute kit (Qiagen ...
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bioRxiv - Systems Biology 2023Quote: ChIP-seq and input libraries were sequenced on the Illumina NextSeq 500 system with a ∼20% phiX spike-in (Illumina, FC-110-3001) to generate 75 bp single-end reads (NextSeq 500/550 High Output v2 kit) ...
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bioRxiv - Cell Biology 2023Quote: ... prior to pooling and sequencing using the Illumina NextSeq 500 platform using a custom primer and the High Output v2 kit (75Lcycles) (Illumina, #FC-404-2005). The library loading concentration was 2.4 pM ...
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bioRxiv - Genomics 2023Quote: ... a total of 0.75 ng of DNA was combined with 2.5 µl 1/35th diluted transposome (Illumina; purchased in kit # FC-121-1011), and 1X Tris Buffer (10X Tris Buffer ...
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bioRxiv - Physiology 2023Quote: ... Each donor library pool was sequenced twice using the Illumina NextSeq500 with NextSeq 500/550 High Output v2 or v2.5 75-cycle kits (Illumina, USA; FC-404-2005). Libraries were sequenced using the following cycle parameters ...
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bioRxiv - Genomics 2023Quote: ... Isolated nuclei were lysed and transposed for 30 minutes at 37°C using the prokaryotic Tn5 transposase system (Nextera DNA library kit, Illumina, FC-121–1030). Transposed DNA was then purified on Diapure columns (Diagenode Cat# C03040001) ...
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bioRxiv - Evolutionary Biology 2023Quote: ... Libraries were constructed by following a previous protocol that optimizes reagent usage for microbial genomes [85] with the Nextera DNA Library Preparation Kit (Illumina, FC-121-1030) and sequenced on an Illumina HiSeq 2500 (UNH ...
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bioRxiv - Developmental Biology 2023Quote: ... and transferred to 3.0 μl Tn5 transposome mixture (0.25 μl Tn5 transposome, 2.5 μl tagmentation buffer, Illumina, FC-121-1030; 0.25 μl H2O). The samples were incubated in 37 °C water bath for 30 min ...
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bioRxiv - Developmental Biology 2023Quote: ... The cDNA was then amplified using 10 cycles and subsequently (0.33 ng) used to construct a pool of uniquely indexed samples using the Nextera XT kit (Illumina, Cat# FC-131-1096). Finally ...
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bioRxiv - Immunology 2023Quote: ... Nuclei were resuspended in the Nextera transposition reaction mix (25 μL 2x TD Buffer, 2.5 μL Nextera Tn5 transposase (Illumina Cat #FC-121-1030), and 22.5 μL nuclease free H2O ...
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bioRxiv - Developmental Biology 2024Quote: ... Approximately 1 ng of cDNA per cell was transformed into a single-cell library using the Nextera XT DNA Library prep kit (Illumina FC-131-1096) and following the manufacturer’s instructions with slight modifications ...
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bioRxiv - Genomics 2024Quote: ... and the final sequencing library was amplified utilizing the KAPA HiFi HotStart Uracil+ ReadyMix PCR Kit along with index primers from the Nextera XT Index Kit (Illumina, FC-131-1001). The amplification protocol involved an initial step at 98°C for 45 seconds ...
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bioRxiv - Microbiology 2024Quote: ... using the standard procedure recommended by the manufacturer with a 0.5% addition of the PhiX control library (Illumina, cat. no. FC-110-3001).
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bioRxiv - Microbiology 2024Quote: ... Sequencing was performed using Pair-end 2×100 cycle mode on the Illumina NovaSeq 6000 system (NovaSeq 6000 S1 Reagent Kit v1.5 200 cycles Illumina, cat. no. 20028317; 0.5% of the PhiX control library, Illumina, cat. no. FC-110-3001) and the standard clustering procedure.
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bioRxiv - Microbiology 2023Quote: ... The cDNA libraries were then fragmented and amplified for sequencing using the Illumina Nextera XT DNA Library Preparation kit (Illumina, FC-131-1096) using custom primers that enabled the specific amplification of only the 3’ ends ...
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bioRxiv - Immunology 2024Quote: ... paired-end libraries were constructed from 1 µg of the initial genomic material using the TruSeq DNA v2 Sample Prep Kit (Illumina, #FC-121-2001) as per the manufacturer’s instructions ...
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bioRxiv - Systems Biology 2020Quote: ... each C1-HT plate was divided into 20 columns of 40 cells each and each well labeled with a position specific barcode and each column was given a separate Nextera XT i7 index (Illumina). The resulting 800 cDNA’s were pooled and NEBNext multiplex oligos for Illumina (the i5 indexes ...
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bioRxiv - Neuroscience 2023Quote: ... biotin-labeled and directly hybridized to the Illumina HumanHT-12 v4.0 Expression BeadChip (47,231 probes) (Illumina, San Diego, CA, USA). Slides were scanned using the Illumina iScan and the signal was extracted and normalized using the GenomeStudio software ...
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bioRxiv - Evolutionary Biology 2022Quote: ... the library was sequenced on Illumina Hiseq platform with cBot System via TruSeq PE (Paired-End) Cluster Kit v3-cBot-HS (Illumina, US). For WGBS data analysis ...
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bioRxiv - Genomics 2020Quote: ... Paired-end (PE) libraries were constructed for the Senzu’s DNA and sequenced using an Illumina HiSeqX system (Illumina, San Diego, CA) with the read-length of 151 bp ...
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bioRxiv - Genomics 2020Quote: ... quantitated by real-time quantitative PCR (qPCR) using a TaqMan® Probe and amplified off-board on a cBot (TruSeq PE Cluster Kit v3–cBot–HS, Illumina) to generate clusters on the sequencing flow cell ...
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bioRxiv - Plant Biology 2019Quote: ... The Hi-C libraries were sent to the Australian Genome Research Facility (Melbourne, Australia) for sequencing using one lane of 100 bp PE sequencing using a HiSeq2000 (Illumina Inc.).
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Mutant ACTB mRNA 3′UTR Promotes Hepatocellular Carcinoma Development by Regulating miR-1 and miR-29abioRxiv - Cancer Biology 2019Quote: ... The clustering of the index-coded samples was performed on a cBot Cluster Generation System using TruSeq PE Cluster Kit v3-cBot-HS (Illumina, USA). After cluster generation ...
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bioRxiv - Genomics 2021Quote: ... The final pool was sequenced on Illumina MiSeq platform 2×300 bp using the MiSeq Reagent Kit V3 (600 cycles PE) cartridge (Illumina, USA).
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bioRxiv - Plant Biology 2020Quote: ... Sequence libraries for PE short reads were constructed using an Illumina TruSeq DNA LT Sample Prep Kit (Illumina K.K., Tokyo, Japan). The libraries were sequenced on a HiSeq high output (150 bp ...
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bioRxiv - Neuroscience 2019Quote: ... Cluster generation of the library was performed on a cBot with Illumina HiSeq PE Cluster Kit v4 – cBot – HS (Illumina, USA). The libraries were sequenced following a paired-end method (100 base pair reads ...
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bioRxiv - Microbiology 2020Quote: ... The final combined library was sequenced using Illumina technology on NextSeq 500 sequencer using mid output 150-bp paired-end (PE) sequencing kit (Illumina, 20024905).
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bioRxiv - Microbiology 2022Quote: ... The clustering of the index-coded samples was performed on a cBot Cluster Generation System using the Illumina PE Cluster Kit (Illumina, USA) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2023Quote: ... Bisulfite converted libraries were sequenced to 35 – 40 million PE-150×150bp clusters per samples using HiSeq 4000 Sequencer (Illumina, CA).
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bioRxiv - Genomics 2022Quote: ... and sequenced on a NextSeq 500 instrument (NextSeq 500/550 High Output Kit v2.5 (Illumina, 20024907, PE 2 x 75 cycles)) ...
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bioRxiv - Genomics 2024Quote: ... the paired-end (PE 150bp) library was generated using the Illumina TruSeq DNA Nano Preparation Kit (Illumina, San Diego, CA, USA), and the library was sequenced on an Illumina HiSeq 2500 platform following the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2021Quote: ... 5ug of RNA from individual tissues were treated with 15 units of RNAse R (Epicentre, Illumina, USA) for 15 minutes at 37 degrees Celsius ...
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bioRxiv - Genomics 2020Quote: ... The B allele frequency (BAF) and the log R ratio (LRR) were extracted from GenomeStudio (Illumina, USA) for representation.