Labshake search
Citations for Illumina :
901 - 950 of 1668 citations for 5 4 Iodophenoxy methyl furan 2 carboxylic acid since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2023Quote: ... 3-5 µg of each sample was treated with Ribozero® rRNA Removal Kit according to the manufacturer’s instructions (Illumina, USA ...
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bioRxiv - Genomics 2023Quote: ... 3) carried no SNP or indel within 50 bp in their 5’ or 3’ flanking regions (Illumina probe design requirement); and 4 ...
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bioRxiv - Microbiology 2023Quote: ... a PhiX spike-in of 2.5–5% was added to the pools (PhiX sequencing control v3; Illumina FC-110- 3001). Samples were run on the Illumina NovaSeq 6000 platform (single-read 1 ×85 cycles and 6 × i7 index cycles).
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bioRxiv - Neuroscience 2022Quote: ... Each pool was sequenced on the Illumina NextSeq 500 after the addition of 5% PhiX sequencing control library (FC-110-3002; Illumina) using the following settings ...
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bioRxiv - Immunology 2024Quote: ... the antibody barcode library was pooled at a ratio of 1:5 with the gene expression library and sequenced on a NextSeq 2000 (Illumina) to an average depth of 34,000 reads per cell.
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bioRxiv - Cell Biology 2023Quote: ... 30 sec at 62°C and a final extension step of 5 min at 62°C) and sequenced using the NOVASeq platform (Illumina).
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bioRxiv - Genetics 2023Quote: ... Plasma was divided from maternal peripheral blood (5[mL) by centrifuging and then 600[μL (Ion Torrent) or 1.4 mL (Illumina CN500) plasma was used to extract cell-free DNA ...
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bioRxiv - Immunology 2023Quote: ... ADT and 3’ Gexp libraries were mixed at the ratio of 1:5 and sequenced on NovaSeq 6000 sequencer (Illumina) with a configuration of 28/8/0/91-bp for cell barcode ...
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bioRxiv - Developmental Biology 2023Quote: ... The samples were pooled at a concentration of 5 nM and run on an Illumina HI-SEQ 2500 sequencer (Illumina) to obtain paired-end reads of 75 bases (PE75) ...
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bioRxiv - Genomics 2023Quote: ... We used 15 mins incubation on ice in the nuclei preparation step and the Tn5 reaction was performed in 50 μl of custom transposition buffer (10 mM Tris pH 8, 5 mM MgCl2 and 10% dimethylformamide) with 2.5 μl Tn5 transposase (Illumina, 20034197) at 37°C while mixing at 1000 rpm for 30 mins ...
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bioRxiv - Microbiology 2023Quote: ... The transposon-specific primer was designed to include (from 5’ to 3’): (i) the “P5” or “P7” flow-cell annealing sequence (Illumina), (ii ...
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bioRxiv - Genomics 2023Quote: ... groups was based on 5 μg of total RNA according to the manufacturer’s protocol using the Illumina HiScanSQ Instrument (Illumina, USA) and sequenced in the same flow cell as paired-end (2 × 100 bp) ...
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bioRxiv - Microbiology 2024Quote: ... All samples were pooled to a final concentration of 5-10 ng/µL and sequenced on a NextSeq 500 (Illumina). The demultiplexed data was assembled and SNPs detected with Pilon software using reference genome sequences.84
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bioRxiv - Microbiology 2022Quote: ... Ribosomal RNA (rRNA) was subsequently depleted from each RNA sample (5 µg each) using the bacterial Ribo-Zero rRNA Removal Kit (Illumina). The integrity of the RNA was evaluated using an RNA 6000 Nano LabChip and an Agilent 2100 Bioanalyzer (Agilent Technologies ...
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bioRxiv - Molecular Biology 2022Quote: ... with Forward Library primer (5’AATGATACGGCGACCACCGAGATCTACACTCTTTCCCTACACGACGCTCTTCCGATCT3’) and Reverse Library primers (5’CAAGCAGAAGACGGCATACGAGATNNNNNNGTGACTGGAGTTCAGACGTGTGCTCTT CCGATC3’, where NNNNNN denotes the barcodes, e.g. Index1 is CGTGAT for Illumina sequencing). Three rounds of agarose gel purification were performed to purify the fragments of 200~650 bp using QIAquick Gel Extraction Kit (Qiagen) ...
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bioRxiv - Microbiology 2023Quote: ... Sequencing was performed at 5 million reads/sample in single-end mode with 150 nt read length on the NextSeq 500 platform (Illumina) using a Mid output sequencing kit ...
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bioRxiv - Microbiology 2023Quote: ... PCR products were purified with AMPure XP purification kit and Sixteen-plexed samples were pooled in approximately equimolar amounts of 5 nM and run in a single and double index reads on a Hiseq 4000 (Illumina).
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bioRxiv - Genomics 2022Quote: Small RNA libraries were prepared starting from 5 µL RNA eluate using the TruSeq Small RNA Library Prep Kit (Illumina, RS-200-0012 ...
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bioRxiv - Cancer Biology 2023Quote: RNA-seq libraries were prepared with 0,5-1 µg of total high quality RNA collected from samples and the Illumina Stranded Total RNA Prep kit (Illumina) according to manufacturer’s instructions ...
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bioRxiv - Cell Biology 2023Quote: ... Illumina sequencing libraries were indexed with unique 5’ and 3’ barcode combinations (up to 384 cells) using the Nextera XT DNA library preparation kit (Illumina). Libraries were pooled and size-selected with 0.9X AmpPure XP beads ...
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bioRxiv - Cancer Biology 2024Quote: ... Purified PCR products were pooled 1:1 and a 5% PhiX DNA spike-in was included and sequenced by Illumina HiSeq PE150 platform at Novogene ...
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bioRxiv - Microbiology 2024Quote: ... The RNA was dephosphorylated in 3’ and then phosphorylated in 5’ to generate cDNA libraries using the NebNext Small RNA Sample Prep kit with 3’ sRNA Adapter (Illumina) according to the manufacturer’s protocol with 12 cycles of PCR amplification in the last step followed by DNA purification with Monarch PCR DNA cleanup kit (NEB) ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: The reverse transcription products (5 μL) were mixed with Eco™ Real-Time PCR System (Illumina, San Diego, CA, USA) and primers ...
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bioRxiv - Developmental Biology 2024Quote: ... Gene expression and CRISPR libraries were prepared according to the CG000510 Chromium NextGEM Single Cell 5’ v2 CRISPR User Guide (Rev B, 10x Genomics) and sequenced on NextSeq and NovaSeq platforms (Illumina).
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bioRxiv - Systems Biology 2024Quote: ... The quality of each sequencing run was controlled by adding up to 5% of denatured and diluted down to 20pM PhiX (Illumina). Demultiplexing of the data by sample using the defined index pairs was performed automatically by Illumina BaseSpace.
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bioRxiv - Evolutionary Biology 2024Quote: ... Denatured libraries were diluted to a final concentration of 12 pM and combined with 5 % PhiX control (V3 cat# 15017666 from Illumina). Paired-end sequencing was performed on an Illumina MiSeq with 2×150 bp reads (MiSeq Reagent Kit v2 600-cycle ...
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bioRxiv - Immunology 2021Quote: ... Libraries were diluted to 2 nM and paired-end sequencing was performed on a HiSeq 2500 sequencer (Illumina). Stimulation libraries were diluted to 3nM and paired-end sequencing was performed on a NovaSeq 6000 (Illumina).
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bioRxiv - Developmental Biology 2021Quote: ... to determine library size and quantification before paired-end (2 × 41 bp) sequencing on a NextSeq 500 (Illumina) platform ...
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bioRxiv - Developmental Biology 2021Quote: ... Adapters were trimmed and low quality sequences removed using trimmomatic and the requisite Illumina adapter sequences (trimmomatic v0.38; PE -threads 2 ILLUMINACLIP:Illumina_TrueSeq_Adapters_PE.fa:2:30:10 HEADCROP:1 MINLEN:31) ...
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bioRxiv - Genomics 2022Quote: Paired-end DNA sequencing (2×151 bp) of the libraries was performed on the NovaSeq 6000 sequencer (Illumina) using the NovaSeq S4 Reagent Kit ...
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bioRxiv - Genomics 2020Quote: ... on Illumina’s official website (ftp://webdata2:[email protected]/downloads/productfiles/humanomniexpress-24/v1-2/infinium-ommexpress-24-v1-2-mamfest-file-csv.zip) and converted it to a VCF format using Illumina’s GTCtoVCF tool (github.com/Illumina/GTCtoVCF).
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bioRxiv - Genomics 2020Quote: ... Paired-end 2×100 bp RNA-sequencing (Illumina TruSeq RNA Library Prep Kit, Illumina HiSeq2000 and Illumina HiSeq4000) was performed ...
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bioRxiv - Genomics 2020Quote: ... The library preparation and strand-specific paired-end sequencing (2 × 150 bp) on a HiSeq 4000 platform (Illumina) was performed by Novogene Co. ...
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bioRxiv - Genomics 2021Quote: ... according to manufacturer”s instructions and sequenced using a 2 × 250-cycle MiSeq Reagent kit v3.0 (Illumina, CA).
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bioRxiv - Microbiology 2021Quote: ... Libraries were then multiplexed and submitted to the sequencing platform (Illumina MiSeq, pair end 2 x 250 bp). Reads with less than 36 bp were discarded and adaptor sequences were removed by Macrogen Inc.
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bioRxiv - Cancer Biology 2020Quote: ... the library was performed pair-end 2×100bp high-throughput sequencing using HiSeq 2500 and Nova-seq (Illumina).
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bioRxiv - Microbiology 2020Quote: ... lLibraries were diluted to 2 nM in 10 mM of TE and samples were sequenced with MiSeq (Illumina) using a paired-end approach ...
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bioRxiv - Plant Biology 2020Quote: ... An initial sequencing run took place on a MiSeq 2 × 300bp run (v3) (Illumina, San Diego, California, USA) at the Field Museum of Natural History ...
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bioRxiv - Microbiology 2020Quote: ... to a final concentration of 2 nM containing 15% PhiX V3 library control (Illumina, San Diego, CA, USA), the library pools were denatured for 5 min in an equal volume of 0.2M NaOH ...
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bioRxiv - Microbiology 2021Quote: Raw data from the Illumina MiSeq was first converted into FASTQ format 2 × 312 paired-end sequence files using the bcl2fastq program (v1.8.4) provided by Illumina. Format conversion was performed without de-multiplexing ...
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bioRxiv - Microbiology 2020Quote: ... Isolated RNA was heat fragmented and libraries were prepared for 2 × 250 nucleotide paired-end sequencing performed (Illumina). Genewiz performed basecalling and read demultiplexing.
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bioRxiv - Microbiology 2020Quote: ... Paired-end sequences of 2 x 150 bp were generated for all libraries on the NovaSeq platform (Illumina). Metagenomic sequence reads are publicly available on the JGI IMG portal.
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bioRxiv - Microbiology 2022Quote: Genomic DNA extraction and the sequencing library preparation for short-read 2×250 bp paired-end MiSeq (Illumina) sequencing was performed as described before (Valcek et al. ...
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bioRxiv - Immunology 2022Quote: ... Equimolar amounts of each library were paired-end sequenced (2 × 38 bp) on a NextSeq 550 instrument (Illumina).
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bioRxiv - Cell Biology 2022Quote: ... Paired-end 2×100 bp RNA-sequencing (Illumina TruSeq RNA Library Prep Kit, Illumina HiSeq2000 and Illumina HiSeq4000) was performed ...
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bioRxiv - Cell Biology 2022Quote: ... Paired-end 2×100 bp RNA-sequencing (Illumina TruSeq RNA Library Prep Kit, Illumina HiSeq2000 and Illumina HiSeq4000) was performed ...
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bioRxiv - Genomics 2020Quote: ... Libraries were sequenced using the NovaSeq SP Reagent kit (2 × 150 cycles) from Illumina (San Diego, CA, USA). Sample L5630 underwent a target enrichment approach where double stranded DNA (synthesized using the QuantiTect Reverse Transcription Kit from Qiagen ...
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bioRxiv - Genomics 2020Quote: ... Finaly libraries were then pooled and diluted to 2 nM and subsequently sequenced using Illumina HiSeq 2500 (Illumina).
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bioRxiv - Microbiology 2021Quote: ... Transposon mutagenesis was performed with the EZ-Tn5
2>Tnp Transposome kit (Epicentre, Illumina, CA, USA) and the insertion site of all mutants was determined via arbitrary PCR (Segev et al ... -
bioRxiv - Cancer Biology 2020Quote: All statistical analyses were performed in R versions 3.5.2 or 3.5.3(23) except p-values for gene ontology and pathway enrichments which were calculated by Illumina’s BaseSpace Correlation Engine and p-values for motif enrichments which were calculated by MEME suite(24) ...