Labshake search
Citations for Illumina :
9401 - 9450 of 9619 citations for C Reactive Protein CRP ELISA Kit 1 Plate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2023Quote: RNA sequencing libraries were produced in parallel from the same RNA samples subjected to CAGE sequencing by the NHLBI DNA sequencing Core at NIH (Bethesda, MD) using the TruSeq Stranded mRNA Library Prep Kit (Illumina, Paired-end 50 bp reads) and sequenced using the NovaSeq6000 Illumina platform ...
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bioRxiv - Microbiology 2023Quote: ... Fragmented and indexed samples were run on the sequencer using a Micro flow cell with the MiSeq Reagent Kit v2 (Illumina; 150-bp paired-end reads) following Illumina’s recommended denaturation and loading procedures.
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bioRxiv - Neuroscience 2023Quote: ... A total of six libraries of samples were pooled at 12 pM with 1% PhiX spike-in and paired-end sequenced with Illumina MiSeq system using Illumina MiSeq Reagent Kit v3 (150-cycle) (Illumina, United States, MS-102-3001). An average of 5.60 Gb data was obtained from 1849.27 K/mm2 mean cluster density in each sequencing run on MiSeq ...
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bioRxiv - Developmental Biology 2023Quote: ... single end sequencing was performed on an Illumina NextSeq 500 sequencer using a 75 bp HO sequencing kit (v2) (Illumina Inc., San Diego, CA, USA), with all libraries run across 2 flowcells ...
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bioRxiv - Cancer Biology 2023Quote: ... paired end sequencing was performed on an Illumina NextSeq 500 sequencer using a 150 bp HO sequencing kit (v2) (Illumina Inc., San Diego, CA, USA) or 50 bp ...
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bioRxiv - Immunology 2023Quote: ... RNA libraries were prepared from a starting quantity of 100 ng high quality RNA using the TruSeq RNA Library Prep Kit v2 (Illumina Inc., San Diego, CA, USA). Individually barcoded RNA-seq libraries were pooled in equimolar quantities and the quantity and quality of the final pooled libraries (two different pools in total ...
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bioRxiv - Systems Biology 2023Quote: ... The resulting amplicon libraries were normalized and sequenced on an Illumina sequencing platform using a MiSeq Reagent Kit V2 (Illumina Inc., San Diego, CA, USA). The final load concentration was 4.0-5.5 pM with a 15% PhiX spike to add diversity.
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bioRxiv - Microbiology 2023Quote: ... A genomic library with average insert size of 350 bp was prepared for sequencing using a TruSeq Nano DNA library preparation kit (Illumina, Inc., San Diego, CA, USA). Paired-end 2 x 125 nt sequencing was performed by the MGX platform (CNRS ...
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bioRxiv - Microbiology 2023Quote: ... The tissue-derived Illumina libraries were sequenced using a 2 x 250 cycle MiSeq Reagent Kit v2 on a MiSeq platform (Illumina Inc., San Diego, CA, USA). The 16S rRNA gene V4-V5 region was amplified from each water sample and sequenced using the Illumina MiSeq platform (Illumina Inc. ...
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bioRxiv - Microbiology 2023Quote: ... and sequenced with a 2×250bp paired-end configuration on an Illumina MiSeq platform applying the MiSeq v2 500-cycle sequencing kit (Illumina Inc., San Diego, CA, USA).
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bioRxiv - Immunology 2023Quote: ... RNA libraries were prepared from a starting quantity of 100 ng high quality RNA using the TruSeq RNA Library Prep Kit v2 (Illumina Inc., San Diego, CA, USA). All RNA samples used for transcriptomic analysis (n = 6 per group ...
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bioRxiv - Immunology 2023Quote: ... RNA-seq libraries were generated via mRNA isolation from total RNA by polyA-positive selection using the TruSeq™ RNA/DNA Library Preparation Kit (Illumina #RS-122-2001, USA) prior to sequencing using the NextSeq™ 550 system (Illumina).
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bioRxiv - Immunology 2023Quote: ... Up to 200pg cDNAs were used to generate the dual index Illumina libraries using Nextera XT DNA Library Prep Kit (Illumina Cat No. FC-131-1096) and Nextera XT Index Kit v2 Set A (Illumina ...
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bioRxiv - Immunology 2023Quote: ... Libraries were prepared using the TruSeq® RNA Sample Prep v2 kit with the IDT for Illumina DNA/RNA UD indexes (Illumina, San Diego, CA, USA). Purification and fragmentation of mRNA ...
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bioRxiv - Cell Biology 2023Quote: ... Purified total RNA was used to generate mRNA libraries using NEBNext Poly(A) mRNA Magnetic Isolation Module and NEBNext Ultra RNA Library Prep kit for Illumina (Illumina Inc., San Diego, CA, USA). Quality and quantity were assessed using an Agilent DNA1000 series chip assay and Invitrogen Qubit HS Kit (Invitrogen) ...
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bioRxiv - Microbiology 2024Quote: ... The pool was run at a final concentration of 10pM on an Illumina MiSeq instrument using MiSeq® Reagent Kit v3 (600 cycle) (Illumina Catalogue FC-102-3003) following the Illumina recommended denaturation and loading recommendations which included a 20% PhiX spike in (PhiX Control v3 Illumina Catalogue FC-110-3001) ...
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bioRxiv - Genetics 2024Quote: ... Samples were preamplified using High-Fidelity 2X PCR Master Mix (New England Bio-labs, #M0541L) and adapters (Illumina Nextera XT Index kit #FC-131-1001). The number of additional cycles was determined by quantitative PCR ...
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bioRxiv - Genomics 2024Quote: ... The libraries were prepared with Ampliseq Library Plus for Illumina kit (Illumina, San Diego, CA, USA and using Illumina’s Ampliseq CD indexes set A(Illumina), and their quality was assessed with High Sensitivity DNA reagents on an Agilent 2100 Bioanalyzer System ...
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bioRxiv - Microbiology 2024Quote: ... Parallel aliquots of the DNA samples used for ONT sequencing were used to prepare Illumina libraries using the Nextera DNA Flex Library Prep Kit as per protocol (Illumina Inc., San Diego, CA, USA). Library quality was measured using an Agilent 4200 TapeStation System as described above ...
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bioRxiv - Developmental Biology 2021Quote: ... cDNA libraries were synthetized from 1 µg of total RNA and sequenced using a HiSeq-3000 platform (Illumina) to obtain the 50 base pairs (bp ...
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bioRxiv - Synthetic Biology 2020Quote: ... before multiplex pooling and single-end sequencing on a 1×75 flow cell on the NextSeq500 platform (Illumina). Total reads were 33 to 65 million per sample.
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bioRxiv - Microbiology 2019Quote: ... Genomic DNA of the strains CBC-1 and HTSH020 was sequenced in house on a MiSeq sequencer (Illumina) with 300 bp paired-end reads using PCR-free library preparation ...
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bioRxiv - Genomics 2021Quote: ... We used one of the cohorts for our main analyses (AEMS450K1, Athero-Express Methylation Study Illumina 450K 1) and the other smaller cohort for replicating the effect size of methylation sex differences (AEMS450K2 ...
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bioRxiv - Systems Biology 2021Quote: ... Samples were multiplexed and sequenced on two identical NextSeq500 lanes using high-output 1×75-bp chemistry (Illumina). The concatenated FASTQ files generated from Illumina were used as input to kallisto ...
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Adaptive translational pausing is a hallmark of the cellular response to severe environmental stressbioRxiv - Molecular Biology 2020Quote: ... 1 µg RNA was used to make libraries with TruSeq Stranded Total RNA Library Prep Gold (Illumina, 20020598) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2021Quote: ... Cells were then lysed and fragmented in a single reaction (12.5 µl 2X Illumina TDE buffer, 2.5 µl 1% Tween-20, 2.5 µl 0.2% Digitonin, 5 µl water, 2.5 µl Illumina TDE1 enzyme). Samples were incubated at 37°C for 60 minutes and purified using the Zymo DNA Clean and Concentrator-5 Kit (Zymo) ...
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bioRxiv - Immunology 2022Quote: ... Multiplexed libraries were sequenced with 1% PhiX control library on the Illumina NextSeq 500 per manufacturer’s protocol (Illumina, Read 1 ...
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bioRxiv - Genomics 2019Quote: ... code at https://github.com/lh3/fermikit in precisionFDA app at https://precision.fda.gov/apps/app-BvJPP100469368x7QvJkKG9Y-1) with default settings from Illumina HiSeq 50x (downsampled to two flow cells ...
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bioRxiv - Microbiology 2019Quote: ... The qPCR efficiency of the assay was calculated (FIG 2) using the following formula: Efficiency = 10[-1/Slope] (http://efficiency.gene-quantification.info/) by Illumina Eco Real-Time PCR system software ...
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bioRxiv - Genetics 2021Quote: ... Sequences were demultiplexed using QIIME2 (core distribution 2018.6)42 for dataset 1 and BCL2FASTQ (Illumina, San Deigo, CA) for dataset 2 ...
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bioRxiv - Molecular Biology 2022Quote: ... Each library was sequenced in 1 × 75 bp of single read mode using a NextSeq 500 platform (Illumina). Adapter sequences are removed from sequencing reads using Trim Galore (version 0.6.7 ...
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bioRxiv - Microbiology 2022Quote: ... 2.5 µL primer P5 (5 µM) and 2.5 µL Index 1 primer (N7**, Illumina, CN FC-131-2001) was used for the PCR enrichment ...
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bioRxiv - Molecular Biology 2023Quote: ... A 2 µl aliquot of the denatured library pool is then added to 1 ml of HT1 (Illumina), mixed and 120 µl is added to the ‘sample library’ cBot strip tube ...
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bioRxiv - Developmental Biology 2023Quote: ... Isolated nuclei were mixed with 50 µl of transposition reaction mix containing Tagment DNA Enzyme 1 (Illumina, 20034197). Samples were incubated using a thermo shaker at 37°C ...
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bioRxiv - Molecular Biology 2023Quote: ... minus (Table 1) was used for whole-genome sequencing using Illumina short-read sequencing technology (Illumina, California, USA). Paired-end libraries with insert sizes of 327–353 bp were sequenced on a NovaSeq 6000 system (Illumina).
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bioRxiv - Genomics 2023Quote: ... indexed using IDT for Illumina Nextera UD indexes Sets 1–4 (384 Indexes, Cat no: 20043137, Illumina, USA) and products were amplified ...
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bioRxiv - Genomics 2024Quote: ... The sRNA-seq libraries were clustered and sequenced (1 × 75 bp) on a NextSeq 550 Sequencing System (Illumina) using a NextSeq 500/550 High Output Kit v2.5 (75 Cycles ...
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bioRxiv - Developmental Biology 2020Quote: ... This service utilizes the Ovation RNA-Seq System V2 kit (Nugen) for initial RNA amplification prior to library preparation and sequencing (Illumina HiSeq v4, 50bp paired-end reads). For each genotype ...
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bioRxiv - Evolutionary Biology 2020Quote: ... modified with 5’-overhangs designed to match the Illumina sequencing adapters and molecular identifiers (MIDs) of the Nextera® XT v2 Index Kit (Illumina Inc., San Diego, CA, USA). The second library included eight technical duplicates ...
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bioRxiv - Evolutionary Biology 2021Quote: ... All of the subsequent steps were performed by the CRG Genomics Unit (Barcelona): sequencing libraries were prepared from the pure high molecular weight DNA using TruSeq DNA HT Library Preparation kit (Illumina ® HiSeq ® Sequencing v4 Chemistry). A paired-end library with a target insert size of ∼500 bp was sequenced on an Illumina® HiSeq2500 platform in paired-end mode ...
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bioRxiv - Developmental Biology 2021Quote: ... We assessed the quality of RNA using fragment analysis to obtain RNA integrity number (RIN) before proceeding with Library preparation (NEBNext® Ultra™ RNA Library Prep Kit, Illumina, San Diego, CA, USA). Each sample was sequenced in Illumina HiSeq 2000 (Single read ...
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bioRxiv - Microbiology 2022Quote: The 16S and ITS libraries were prepared by the sequencing facility using the Illumina TruSeq≡Nano DNA Library Prep Kit (Illumina, Inc., San Diego, CA, United States), llumina MiSeq was run using a v2 Standard sequencing format with paired end reads (2 x 250 bp) ...
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bioRxiv - Molecular Biology 2021Quote: ... DNA quality control was performed in a fragment analyzer and sequencing libraries were constructed using a Nextera DNA Flex kit (now renamed to Illumina DNA Prep, Illumina, San Diego, CA) and 2×150 BP paired-end sequencing was performed in an Illumina NextSeq500 ...
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bioRxiv - Genomics 2020Quote: A total of 720 metagenomic libraries were prepared using Illumina-specific Nextera DNA transposase adapters and a Tagment DNA Enzyme and Buffer Kit (Illumina, San Diego, CA, cat. no. 20034197) (modified from Baym et al ...
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bioRxiv - Systems Biology 2020Quote: ... Supernatants were discarded and nuclei were re-suspended in 50 μl reaction buffer (2.5 μl of Tn5 transposase and 25 μl of TD buffer from a Nextera DNA Library Prep Kit from Illumina, and 22.5 μl nuclease-free H2O). The reaction was incubated at 37°C for 30 min ...
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bioRxiv - Microbiology 2020Quote: ... A second PCR was performed to attach dual indices and Illumina® sequencing adapters using the Nextera XT Index kit (Illumina®; San Diego, CA, USA). The conditions of this PCR included ...
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bioRxiv - Microbiology 2020Quote: ... WGS libraries were sequenced with paired-end 150 base pair reads on an Illumina NovaSeq 6000 by the UW Biotechnology Center (Illumina NovaSeq 6000 S1 Reagent Kit v1.5).
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bioRxiv - Cell Biology 2019Quote: ... transcripts were purified from total RNA and mRNA sequencing libraries were prepared with the TruSeq Stranded mRNA Library Preparation Kit (Illumina, RS-122-2101, RS- 122-2102). The detailed protocol for RNA sequencing was discussed in earlier (34 ...
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bioRxiv - Microbiology 2019Quote: ... A paired-end 250-bp sequencing run was performed using the Illumina MiSeq instrument according to the MiSeq™ Reagent Kit v2 Preparation Guide (Illumina, Inc., San Diego, CA, USA).
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bioRxiv - Microbiology 2023Quote: ... and short read sequencing using the Illumina platform (Illumina DNA Prep kit and IDT 10bp UDI indices, and sequenced on an Illumina NextSeq 2000 producing 2×151bp reads) was performed by the Microbial Genome Sequencing Center ...