Labshake search
Citations for Illumina :
9201 - 9250 of 9619 citations for C Reactive Protein CRP ELISA Kit 1 Plate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2021Quote: ... 100-500 ng of total RNA with RIN values of 9.5-10 underwent polyA selection and TruSeq library preparation according to instructions provided by Illumina (TruSeq Stranded mRNA LT Kit), with 8 cycles of PCR ...
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bioRxiv - Genomics 2021Quote: ... For long read sequencing (Oxford Nanopore Technologies) total RNA was extracted as described above and depleted using the Ribo-Zero rRNA Removal Kit Plant Leaves (Illumina, San Diego, CA, USA). RNA was then purified and concentrated on a RNA Clean Concentrator™-5 column (Zymo Research ...
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bioRxiv - Genomics 2021Quote: Six Illumina RNA-Seq libraries (one for each organ) were constructed from 500ng total RNA using the TruSeq Stranded mRNA kit (Illumina, San Diego, CA, USA), which allows for mRNA strand orientation (the orientation of sequences relative to the antisense strand is recorded) ...
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bioRxiv - Cancer Biology 2020Quote: ... Sequencing was performed using the NextSeq 500/550 High-Output v2 (150 cycle) Kit (# FC-404-2002) on the NextSeq 550 platform (Illumina Inc, #SY-415-1002). Libraries were combined in an equimolar pool based on the library quantification results and run across 5 High-Output Flow Cell v2.5.
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bioRxiv - Developmental Biology 2021Quote: ... and sequencing libraries of poly(A)+ RNAs were then generated using the VAHTSTM mRNA-seq v2 Library Prep Kit from Illumina (Vazyme Biotech, NR601-01) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: ... Dual barcode indices and sequencing adaptors were attached to each amplicon using the Illumina Nextera XT Index kit (Illumina, Inc., San Diego, CA, USA) following manufacturer’s protocol ...
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bioRxiv - Genomics 2020Quote: ... Illumina sequencing was carried out via amplification of the V4 hypervariable region of the 16S rRNA gene with the Earth Microbiome modified F515/R806 primers (22) on a MiSeq platform using a MiSeq 600 cycle kit (Illumina, San Diego, CA, USA). Analysis of the V4 regions was carried out using QIIME2 (21) ...
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bioRxiv - Genomics 2020Quote: ... BS-converted DNA (100 ng) of each sample was used for preparing the BS-seq library via the TruSeq DNA Methylation Kit (Illumina, EGMK91324, San Diego, CA). The quality of the libraries was checked using Qubit assays (Q32850 ...
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bioRxiv - Immunology 2020Quote: ... was adjusted to 1 μg and used for poly (A) mRNA enrichment and stranded specific RNA-Seq library preparation with TruSeq Stranded mRNA kit (Illumina, San Diego, CA, USA). Quality control of prepared libraries were performed using Agilent Bioanalyzer DNA 1000 kit and Qubit DNA HS fluorescence kit ...
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bioRxiv - Microbiology 2020Quote: ... Five DNA extractions were performed per soil sample and pooled for metagenomic library preparation with the TrueSeq DNA kit (Illumina Inc., San Diego, CA). Sequencing was performed on the Illumina HiSeq platform to generate 6.4 billion of paired-end reads of 150bp.
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bioRxiv - Immunology 2021Quote: ... The V(D)J enriched library was then constructed via Chromium Single Cell 5’ Library Construction Kit (10x Genomics Cat#1000020) and libraries were sequenced in a NovaSeq™ 6000 Sequencing System (Illumina, San Diego, CA). V(D)J sequences were collapsed using Cell Ranger ...
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bioRxiv - Molecular Biology 2021Quote: ... Libraries for WGS were prepared using Nextera XT DNA Library Prep Kit and sequencing was performed on the Illumina NextSeq500 machine as per manufacturer’s protocol (Illumina Inc., San Diego, California, USA) producing 2 x 151 base pair reads.
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bioRxiv - Cancer Biology 2020Quote: ... Strand specific RNA-seq libraries were constructed using the Illumina TruSeq Stranded Total RNA Library preparation Gold kit (20020598, Illumina Inc., San Diego, CA) as per the instructions ...
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bioRxiv - Immunology 2021Quote: ... Library preparation was done using a NEBNext Ultra II Directional RNA Library Prep Kit for Illumina with poly-A selection (Illumina, San Diego, CA, USA) following the manufacturer’s instructions ...
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bioRxiv - Immunology 2021Quote: Library preparation for sequencing was performed using 200 ngs of total RNA input with the TrueSeq RNA Sample Prep Kit v2-Set B (RS-122-2002, Illumina Inc, San Diego, CA) producing a 275bp Fragment including Adapters in average size ...
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bioRxiv - Physiology 2020Quote: ... 200ng of RNA per sample were used as the input of TruSeq Stranded mRNA Library Prep kit to make barcoded libraries according to the manufacturer’s protocol (Illumina Inc., San Diego, CA, USA). The library concentration was determined by qPCR using the Library Quantification Kit Illumina Platforms (Kapa Biosystems ...
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bioRxiv - Genomics 2021Quote: ... Paired-end sequencing libraries were prepared using the TruSeq Nano DNA Library Prep kit following the manufacturer’s specifications (Illumina Inc., San Diego, CA, USA). Out of the 150 libraries ...
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bioRxiv - Genomics 2021Quote: ... Paired-end libraries were constructed from the double stranded cDNA by using Illumina DNA Prep with Enrichment kit (Illumina Inc., San Diego, CA, USA) following the manufacturer’s protocol ...
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bioRxiv - Genomics 2020Quote: ... Paired-end sequencing libraries were constructed with an insert size of 500 bp using a TruSeq DNA PCR-Free kit (Illumina, San Diego, CA, USA) according to Illumina library preparation protocols ...
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bioRxiv - Genomics 2020Quote: ... and 8 to 11 kb were generated using the gel selection-based protocol of the Nextera mate pair kit (Illumina, San Diego, CA, USA) and a 0.6% agarose gel in accordance with the manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: ... the amplicons were purified and sequenced on a MiSeq flow cell using Reagent Kit v3 and 2×300 pair-end reads (Illumina Inc., San Diego, USA) according to the manufacturer’s instructions at the Göttingen Genomics Laboratory (G2L ...
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bioRxiv - Cancer Biology 2021Quote: ... sequencing libraries using total RNA generated from frozen tissues with a TruSeq mRNA Library Preparation Kit using poly-A-enriched RNA (Illumina, San Diego, CA, USA) and enriched with the human kinome DNA capture baits (Agilent Technologies ...
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bioRxiv - Genomics 2019Quote: ... Libraries for samples with a sufficient RNA integrity value were prepared using the Illumina TruSeq Total RNA Sample Prep Kit (Illumina, Inc., San Diego, USA) with ribosomal depletion ...
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bioRxiv - Microbiology 2020Quote: ... Illumina MiSeq sequencing was performed at the Genome Institute of Singapore according to the MiSeq reagent kit v2 (2 × 250 bp) preparation guide (Illumina, San Diego, CA, USA).
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bioRxiv - Cancer Biology 2022Quote: ... and sequenced on the Illumina NextSeq 2000 sequencer with the P3-200 cycle reagent kit in order to generate approximately 50M million 100-base read pairs per sample (Illumina, Inc., San Diego, CA).
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bioRxiv - Cell Biology 2022Quote: ... an average of 500 pg of amplified cDNA was used to prepare the libraries following Nextera XT DNA kit (Illumina Ref. FC-131-1096). Libraries were multiplexed by 6 on a high-output flow cells ...
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bioRxiv - Cell Biology 2022Quote: ... as described in the section “Quantitative real-time PCR.” The library was prepared using the TruSeq stranded mRNA sample prep kit (Illumina, San Diego, CA, USA) according to the manufacturer’s instructions and sequenced on an Illumina NovaSeq 6000 platform in the 101 bp single-end mode ...
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bioRxiv - Genomics 2022Quote: ... Short-read sequencing data for error correction was generated from the same individual using the TruSeq Nano DNA Low Throughput Library Prep Kit and IDT for Illumina – TruSeq DNA UD Indexes (Illumina, San Diego, CA, USA). Standard protocols were followed to yield a paired library with a 350 bp insert size and 50 bp standard deviation ...
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bioRxiv - Genomics 2022Quote: ... Genomic DNA library was prepared using the Chromium Genome Library Kit v2 (10X Genomics, Pleasanton, CA, USA) and sequenced on the NovaSeq 6000 platform (Illumina, San Diego, CA, USA) to generate paired-end 150 bp reads ...
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bioRxiv - Evolutionary Biology 2022Quote: ... was used for cDNA synthesis and purification using a low-throughput protocol with a TruSeq Stranded RNA LT Kit (Illumina, San Diego, CA, USA). A half-scale reaction of the standard protocol was used for library preparation ...
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bioRxiv - Plant Biology 2022Quote: ... We prepare 12 cDNA libraries (3 individuals □ 2 sampling times (dawn and dusk) □ 2 localities) using the TruSeq RNA-seq library prep kit from Illumina (Illumina, Inc., CA, USA) according to manufacturer’s instructions ...
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bioRxiv - Physiology 2022Quote: ... and used to construct stranded RNA-seq libraries from polyA-selected total liver RNA using an Illumina stranded TruSeq mRNA Prep Kit (Illumina, San Diego, CA, USA). Three pooled RNA samples were prepared for each of the four treatment groups ...
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bioRxiv - Microbiology 2022Quote: ... Purified PCR products were fragmented and tagged with the indexed adaptors using the Nextera DNA XT Sample Preparation Kit (Illumina, San Diego, CA, USA). The sequence library was quantified by using the Quant-iT PicoGreen dsDNA Assay Kit (Invitrogen) ...
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bioRxiv - Microbiology 2022Quote: ... Libraries were pooled and sequenced (single-end 75 bp reads) on Illumina NextSeq 500 using High Output V2 Kit (Illumina, San Diego, CA, USA). Sequencing data were analyzed as described previously (70 ...
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bioRxiv - Microbiology 2022Quote: ... Sequence reads were obtained from the Illumina NovaSeq platform based on 2 × 250 bp cycles and the NovaSeq 6000 SP Reagent Kit (500 cycles, Illumina, San Diego CA, USA). Further analysis was performed using the QIIME 2 standard pipeline (Version 2020.11)45.
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bioRxiv - Microbiology 2022Quote: ... Amplicons of the V3-V4 region of the 16S rRNA gene and the and the ITS1 region and library prep were generated by Herculase II Fusion DNA Polymerase Nextera XT Index Kit V2 (Illumina, San Diego, CA, USA). The products of the library prep were validated by an Agilent Tapestation D1000 (Santa Clara ...
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bioRxiv - Microbiology 2022Quote: ... 16S rRNA genes were PCR-amplified using primers 341f and 785r [29] and sequenced on the Illumina Miseq platform (Miseq Reagent Kit v3, 2×300 bp; Illumina, San Diego, CA, USA).
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bioRxiv - Microbiology 2022Quote: ... Indexed amplicons were pooled in equimolar amounts and sequenced using Illumina’s v3 600-cycle (paired-end) reagent kit on an Illumina MiSeq benchtop sequencer (Illumina Inc., San Diego, CA, USA) after all DNA extraction blanks and PCR reagent blanks were confirmed for negative amplification.
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bioRxiv - Microbiology 2022Quote: Short read sequencing libraries were prepared using the Illumina DNA Prep kit in combination with IDT® UD Indexes Set A (Illumina, USA, #20018705, #20027213). Between 66.3 ng and 200 ng per sample was used as starting material ...
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bioRxiv - Neuroscience 2022Quote: ... Sequencing libraries were prepared by transposase-assisted tagmentation and enrichment of 3’end of genes using Nextera XT DNA Library Prep Kit (Illumina, San Diego, CA, USA). The libraries were sequenced on the HiSeq2500 for 25 and 75 cycles paired-end reads ...
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bioRxiv - Molecular Biology 2023Quote: ... Sequencing was performed by the NIDDK Genomics Core facility using a MiSeq system with the single-end 50 bp Sequencing Kit (Illumina, San Diego, CA, USA). Sequence reads were mapped to the reference genome (NC_000913.3 ...
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bioRxiv - Plant Biology 2022Quote: ... Illumina sequencing pair-end libraries with an insert size of 300 bp were prepared using the Nextera DNA Flex Library Prep Kit (Illumina, San Diego, CA, USA). After the libraries were constructed ...
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bioRxiv - Genomics 2022Quote: ... Mixed nucleic acid (DNA and cDNA) from all samples were subjected to library construction using Nextera XT DNA Sample Preparation Kit (Illumina Inc., San Diego, USA) and sequenced on an Illumina MiSeq platform (Illumina ...
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bioRxiv - Microbiology 2023Quote: ... 5 µl of this reaction was used in a subsequent round of amplification using BC amp set 3 primers to add Illumina adapters to the sequences followed by amplification using the Nextera XT DNA Library Preparation Kit (Illumina cat. FC-131-1096) to add 5’ and 3’ indices to the library ...
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bioRxiv - Neuroscience 2023Quote: ... for 16p11.2 del/+ mice were prepared using the Illumina TruSeq Stranded Total RNA with Ribo-Zero gold sample preparation kit (Illumina, Inc., San Diego, CA). Pooled libraries were sequenced on Illumina NovaSeq 6000 sequencers with 100-bp Paired-End chemistry (Illumina) ...
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bioRxiv - Cancer Biology 2023Quote: ... Libraries were pooled and single end sequenced (1X75) on the Illumina NextSeq 500 using the High output V2 kit (Illumina Inc., San Diego CA). Read data was processed in BaseSpace (basespace.illumina.com) ...
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bioRxiv - Microbiology 2022Quote: ... Whole genome metagenomic libraries were prepared at LLNL using the Illumina DNA Flex library kit (now called Illumina DNA Prep, Illumina Inc., Santa Clara, CA) using 1 ng of sample DNA and 12 cycles of amplification ...
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bioRxiv - Neuroscience 2022Quote: ... Approximately 1 ug of total RNA was then used to prepare small RNA library according to the protocol of TruSeq Small RNA Sample Prep Kits (Illumina, San Diego, CA, USA). Then ...
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bioRxiv - Microbiology 2022Quote: ... multiplexed at 72-96 samples per run in paired-end mode with 325 × 275 cycles using the MiSeq Reagent v3 600-cycle kit (Illumina Inc., San Diego, CA).
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bioRxiv - Bioengineering 2024Quote: Barcoded stranded mRNA-seq libraries were prepared from total RNA (∼200 ng) using the Illumina TruSeq stranded mRNA Sample Preparation v2 Kit (Illumina, San Diego, CA, USA) implemented on the liquid handling robot Beckman FXP2 ...