Labshake search
Citations for Illumina :
9251 - 9300 of 9493 citations for Rat Hypoxanthine Phosphoribosyltransferase 1 HPRT1 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2020Quote: ... the purified sample was used as the template for barcoding with Nextera XT Index Kit v2 (FC-131-1002 - Illumina Inc, San Diego, CA, USA). The finished libraries were normalized to 4 nM and pooled ...
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bioRxiv - Plant Biology 2021Quote: ... were randomly pooled (4 samples per pool) and grouped using the TruSeq Paired-End Reads Cluster Kit on the cBot platform (Illumina Inc., San Diego, CA, USA). The cDNA libraries were posteriorly sequenced on the Illumina Genome platform Analyzer IIx with a TruSeq kit with 36 cycles (Illumina ...
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bioRxiv - Molecular Biology 2022Quote: ... Bulk and STORM-seq K562 libraries were pooled and 2×50 bp sequencing was performed on an Illumina NovaSeq 6000 sequencer using a 100 bp S2 sequencing kit (Illumina Inc., San Diego, CA, USA). The HEK293T ...
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bioRxiv - Cancer Biology 2019Quote: All libraries were sequenced using the Illumina HiSeq 2000/2500 system with TruSeq SBS Kit v3 - HS (200-cycles) reagents (Illumina, Part no. FC-401-3001), to generate paired-end 101 bp reads.
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bioRxiv - Cancer Biology 2019Quote: Whole-genome libraries were generated using either the Illumina TruSeq DNA LT sample preparation kit (Illumina, Part no. FC-121–2001 and FC-121–2001) or the Illumina TruSeq DNA PCR-free LT sample preparation kit (Illumina ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... The pooled TempO-Seq® library was sequenced in-house using the NextSeq® 500/550 High Output Kits v2 (75 cycles) on an Illumina NextSeq 500 High-Throughput Sequencing System (Illumina, San Diego, CA, USA). A median read depth of 2 million reads/sample was achieved.
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bioRxiv - Microbiology 2019Quote: ... Paired-end libraries of 150-bp read length were prepared using the Nextera XT DNA Sample Preparation kit (Illumina Inc., San Diego, 160 CA, USA) and sequenced on Illumina MiniSeq using standard procedures ...
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bioRxiv - Plant Biology 2019Quote: The RNA samples were used to generate sequencing libraries according to the manufacturer of Illumina TruSeq™ RNA Sample Preparation Kits (Illumina, San Diego, CA, USA) Transcriptome sequencing was carried out on an Illumina HiSeq 2500 platform that produced 150 base pair (bp ...
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bioRxiv - Genomics 2019Quote: ... The rRNA-depleted RNA was then used to generate strand-specific RNA-seq libraries using TruSeq® Stranded mRNA Kit (Illumina, San Diego, CA, USA). In total ...
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bioRxiv - Microbiology 2021Quote: ... An Illumina Nextera XT library was prepared for paired-end sequencing on an Illumina NextSeq 500 with a Mid Output v2 kit (300 cycles) (Illumina Inc., San Diego, CA, USA). Paired-end reads (2×151 bp ...
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bioRxiv - Plant Biology 2021Quote: ... and mRNA-seq libraries were constructed and sequenced by the University of Missouri DNA Core Facility using the TruSeq Stranded mRNA Sample Prep Kit (Illumina, Inc., San Diego, CA, USA) and the Illumina HiSeq and NextSeq platforms.
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bioRxiv - Microbiology 2021Quote: ... A library was prepared by adaptor ligation with the PCR primer pairs using the TruSeq Nano DNA Library Prep Kit (Illumina, Inc., San Diego, CA, USA). When two or more bands were detected using 1.5%-agarose gel electrophoresis ...
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bioRxiv - Immunology 2021Quote: ... Paired end (2×300 cycle) sequencing was performed on a Miseq® sequencer using a 600 cycle v3 Reagent Kit (Illumina Inc., San Diego CA) producing 300 base paired end reads.
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bioRxiv - Cell Biology 2022Quote: ... Tagmentation products were amplified by PCR by adding 1.25 µl of each N and S primers (Illumina Nextera XT 96-index kit FC-131- 1002) and 3.75 µl of NPM solution and using the following thermocycler settings ...
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bioRxiv - Microbiology 2022Quote: ... Fragmented and indexed samples were run on the sequencer using a Micro flow cell with the MiSeq Reagent Kit v2 (Illumina; 150-bp paired-end reads) following Illumina’s recommended denaturation and loading procedures ...
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bioRxiv - Neuroscience 2022Quote: ... Transcriptomic profile of individual BAT samples was performed using commercial RNA-sequencing kits (NEBNext mRNA Library Prep Master Mix and NEBNext Multiplex Oligos for Illumina, New England Biolabs, Ipswich, MA) and adapted according to previous descriptions [22] ...
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bioRxiv - Genomics 2024Quote: ... Ltd (Hong Kong, China) for polyA selected RNA sequencing library construction using the TruSeq RNA Sample Prep Kit v2 (Illumina Cat. No. RS-122-2001), and 150 bp paired-end (PE ...
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bioRxiv - Microbiology 2023Quote: ... Library preparation for VLP genomic DNA involved using >25ng input DNA with the Nextera DNA Flex Library Preparation Kit (currently named Illumina DNA prep, Illumina, CA, USA). Deviating from the Illumina protocol’s full reaction volume (50 µL) ...
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bioRxiv - Neuroscience 2024Quote: ... Purified total RNA was used to generate mRNA libraries using NEBNext Poly(A) mRNA Magnetic Isolation Module and NEBNext Ultra RNA Library Prep kit for Illumina (Illumina Inc., San Diego, CA, USA). Quality and quantity were assessed using an Agilent DNA1000 series chip assay and Invitrogen Qubit HS Kit (Invitrogen) ...
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bioRxiv - Microbiology 2024Quote: Extracted DNA and cDNA were submitted to Georgia Institute of Technology’s Molecular Evolution Core where for sequencing using the Illumina MiSeq v3 kit PE300 using Illumina Nextera Adapter Sequences (Illumina Inc, San Diego, California, USA). The samples were demultiplexed and barcodes removed by the sequencing core ...
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bioRxiv - Genomics 2023Quote: ... libraries were prepared from liver tissues using an Illumina TruSeq Nano DNA Library Prep Kit and paired-end (PE150) sequenced on the Illumina Novaseq 6000 sequencing platform (Illumina, Inc., San Diego, CA, USA). We used gonad tissues for ONT PromethION library preparation and sequencing ...
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bioRxiv - Genomics 2024Quote: ... were used to prepare Illumina libraries using the Nextera DNA Flex Library Prep Kit according to the manufacturers’ instructions (Illumina Inc. San Diego, CA, USA). Library quality was measured via electrophoresis in D1000 ScreenTape on an Agilent 4200 TapeStation System (Agilent Technologies Netherlands BV ...
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bioRxiv - Genomics 2024Quote: ... Ltd (Hong Kong, China) for polyA selected RNA sequencing library construction using the TruSeq RNA Sample Prep Kit v2 (Illumina Cat. No. RS-122-2001), and 150 bp paired-end (PE ...
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bioRxiv - Molecular Biology 2023Quote: RNA sequencing libraries were produced in parallel from the same RNA samples subjected to CAGE sequencing by the NHLBI DNA sequencing Core at NIH (Bethesda, MD) using the TruSeq Stranded mRNA Library Prep Kit (Illumina, Paired-end 50 bp reads) and sequenced using the NovaSeq6000 Illumina platform ...
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bioRxiv - Microbiology 2023Quote: ... Fragmented and indexed samples were run on the sequencer using a Micro flow cell with the MiSeq Reagent Kit v2 (Illumina; 150-bp paired-end reads) following Illumina’s recommended denaturation and loading procedures.
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bioRxiv - Neuroscience 2023Quote: ... A total of six libraries of samples were pooled at 12 pM with 1% PhiX spike-in and paired-end sequenced with Illumina MiSeq system using Illumina MiSeq Reagent Kit v3 (150-cycle) (Illumina, United States, MS-102-3001). An average of 5.60 Gb data was obtained from 1849.27 K/mm2 mean cluster density in each sequencing run on MiSeq ...
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bioRxiv - Developmental Biology 2023Quote: ... single end sequencing was performed on an Illumina NextSeq 500 sequencer using a 75 bp HO sequencing kit (v2) (Illumina Inc., San Diego, CA, USA), with all libraries run across 2 flowcells ...
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bioRxiv - Cancer Biology 2023Quote: ... paired end sequencing was performed on an Illumina NextSeq 500 sequencer using a 150 bp HO sequencing kit (v2) (Illumina Inc., San Diego, CA, USA) or 50 bp ...
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bioRxiv - Immunology 2023Quote: ... RNA libraries were prepared from a starting quantity of 100 ng high quality RNA using the TruSeq RNA Library Prep Kit v2 (Illumina Inc., San Diego, CA, USA). Individually barcoded RNA-seq libraries were pooled in equimolar quantities and the quantity and quality of the final pooled libraries (two different pools in total ...
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bioRxiv - Systems Biology 2023Quote: ... The resulting amplicon libraries were normalized and sequenced on an Illumina sequencing platform using a MiSeq Reagent Kit V2 (Illumina Inc., San Diego, CA, USA). The final load concentration was 4.0-5.5 pM with a 15% PhiX spike to add diversity.
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bioRxiv - Microbiology 2023Quote: ... A genomic library with average insert size of 350 bp was prepared for sequencing using a TruSeq Nano DNA library preparation kit (Illumina, Inc., San Diego, CA, USA). Paired-end 2 x 125 nt sequencing was performed by the MGX platform (CNRS ...
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bioRxiv - Microbiology 2023Quote: ... The tissue-derived Illumina libraries were sequenced using a 2 x 250 cycle MiSeq Reagent Kit v2 on a MiSeq platform (Illumina Inc., San Diego, CA, USA). The 16S rRNA gene V4-V5 region was amplified from each water sample and sequenced using the Illumina MiSeq platform (Illumina Inc. ...
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bioRxiv - Microbiology 2023Quote: ... and sequenced with a 2×250bp paired-end configuration on an Illumina MiSeq platform applying the MiSeq v2 500-cycle sequencing kit (Illumina Inc., San Diego, CA, USA).
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bioRxiv - Immunology 2023Quote: ... RNA libraries were prepared from a starting quantity of 100 ng high quality RNA using the TruSeq RNA Library Prep Kit v2 (Illumina Inc., San Diego, CA, USA). All RNA samples used for transcriptomic analysis (n = 6 per group ...
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bioRxiv - Immunology 2023Quote: ... RNA-seq libraries were generated via mRNA isolation from total RNA by polyA-positive selection using the TruSeq™ RNA/DNA Library Preparation Kit (Illumina #RS-122-2001, USA) prior to sequencing using the NextSeq™ 550 system (Illumina).
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bioRxiv - Immunology 2023Quote: ... Up to 200pg cDNAs were used to generate the dual index Illumina libraries using Nextera XT DNA Library Prep Kit (Illumina Cat No. FC-131-1096) and Nextera XT Index Kit v2 Set A (Illumina ...
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bioRxiv - Immunology 2023Quote: ... Libraries were prepared using the TruSeq® RNA Sample Prep v2 kit with the IDT for Illumina DNA/RNA UD indexes (Illumina, San Diego, CA, USA). Purification and fragmentation of mRNA ...
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bioRxiv - Cell Biology 2023Quote: ... Purified total RNA was used to generate mRNA libraries using NEBNext Poly(A) mRNA Magnetic Isolation Module and NEBNext Ultra RNA Library Prep kit for Illumina (Illumina Inc., San Diego, CA, USA). Quality and quantity were assessed using an Agilent DNA1000 series chip assay and Invitrogen Qubit HS Kit (Invitrogen) ...
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bioRxiv - Microbiology 2024Quote: ... The pool was run at a final concentration of 10pM on an Illumina MiSeq instrument using MiSeq® Reagent Kit v3 (600 cycle) (Illumina Catalogue FC-102-3003) following the Illumina recommended denaturation and loading recommendations which included a 20% PhiX spike in (PhiX Control v3 Illumina Catalogue FC-110-3001) ...
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bioRxiv - Plant Biology 2024Quote: ... The subsequent steps of library preparation were all carried out on the Agilent NGS Bravo workstation in 96-well plates following the instructions for the Illumina TruSeq Stranded mRNA kit (Illumina Cat no: 20020595, Illumina, USA). mRNA was purified from 300-800 ng of total RNA through selective-binding on poly dT-coated beads ...
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bioRxiv - Genetics 2024Quote: ... Samples were preamplified using High-Fidelity 2X PCR Master Mix (New England Bio-labs, #M0541L) and adapters (Illumina Nextera XT Index kit #FC-131-1001). The number of additional cycles was determined by quantitative PCR ...
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bioRxiv - Genomics 2024Quote: ... The libraries were prepared with Ampliseq Library Plus for Illumina kit (Illumina, San Diego, CA, USA and using Illumina’s Ampliseq CD indexes set A(Illumina), and their quality was assessed with High Sensitivity DNA reagents on an Agilent 2100 Bioanalyzer System ...
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bioRxiv - Microbiology 2024Quote: ... Parallel aliquots of the DNA samples used for ONT sequencing were used to prepare Illumina libraries using the Nextera DNA Flex Library Prep Kit as per protocol (Illumina Inc., San Diego, CA, USA). Library quality was measured using an Agilent 4200 TapeStation System as described above ...
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bioRxiv - Developmental Biology 2021Quote: ... cDNA libraries were synthetized from 1 µg of total RNA and sequenced using a HiSeq-3000 platform (Illumina) to obtain the 50 base pairs (bp ...
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bioRxiv - Synthetic Biology 2020Quote: ... before multiplex pooling and single-end sequencing on a 1×75 flow cell on the NextSeq500 platform (Illumina). Total reads were 33 to 65 million per sample.
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bioRxiv - Microbiology 2019Quote: ... Genomic DNA of the strains CBC-1 and HTSH020 was sequenced in house on a MiSeq sequencer (Illumina) with 300 bp paired-end reads using PCR-free library preparation ...
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bioRxiv - Genomics 2021Quote: ... We used one of the cohorts for our main analyses (AEMS450K1, Athero-Express Methylation Study Illumina 450K 1) and the other smaller cohort for replicating the effect size of methylation sex differences (AEMS450K2 ...
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bioRxiv - Systems Biology 2021Quote: ... Samples were multiplexed and sequenced on two identical NextSeq500 lanes using high-output 1×75-bp chemistry (Illumina). The concatenated FASTQ files generated from Illumina were used as input to kallisto ...
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Adaptive translational pausing is a hallmark of the cellular response to severe environmental stressbioRxiv - Molecular Biology 2020Quote: ... 1 µg RNA was used to make libraries with TruSeq Stranded Total RNA Library Prep Gold (Illumina, 20020598) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2021Quote: ... Cells were then lysed and fragmented in a single reaction (12.5 µl 2X Illumina TDE buffer, 2.5 µl 1% Tween-20, 2.5 µl 0.2% Digitonin, 5 µl water, 2.5 µl Illumina TDE1 enzyme). Samples were incubated at 37°C for 60 minutes and purified using the Zymo DNA Clean and Concentrator-5 Kit (Zymo) ...