Labshake search
Citations for Illumina :
851 - 900 of 1186 citations for 4' Trifluoromethoxy 5 trifluoromethyl 1 1' biphenyl 3 carboxylic acid since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genomics 2021Quote: ... The final libraries at the concentration of 4 nM were sequenced on NextSeq 500 platform (Illumina, CA, USA) using 75 bp paired-end sequencing ...
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bioRxiv - Molecular Biology 2021Quote: ... 4 μg total RNA per sample were used for the TruSeq Stranded mRNA LT Sample Prep Kit (Illumina) to generate cDNA libraries according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2022Quote: ... Six samples per condition containing 4 μg of total RNA were used for TruSeq mRNA library preparation (Illumina) after being treated with Globin-Zero Gold rRNA Removal Kit (Illumina) ...
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bioRxiv - Cell Biology 2023Quote: ... Libraries were pooled together and sequenced using 75bp paired-end chemistry across 4 lanes of a Hiseq4000instrument (Illumina) to achieve 10 million reads per sample ...
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bioRxiv - Genomics 2022Quote: ... The additional 71 ASH donors were genotyped separately at 4,327,108 SNPs with the Infinium Omni5-4 v1.2 BeadChip (Illumina, California). We updated SNP identifiers based on Illumina annotation files (https://support.illumina.com/content/dam/illumina-support/documents/downloads/productfiles/humanomni5-4/v1-2/infinium-omni5-4-v1-2-a1-b144-rsids.zip ...
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bioRxiv - Immunology 2022Quote: ... High-resolution 4-digit HLA typing was performed with the TruSight HLA v2 Sequencing Panel kit from Illumina according to the manufacturer instruction ...
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bioRxiv - Genomics 2024Quote: ... Equal molar concentration sequencing libraries were pooled and set in 4 sequencing lanes of a NextSeq500 equipment (Illumina) run to avoid possible batch effects (Li et al. ...
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bioRxiv - Cell Biology 2020Quote: ... an oligonucleotide containing the Illumina P7 adaptor sequence was ligated to the 3’ end of the single stranded LAM PCR fragments using Circligase (Epicentre/Illumina, #CL9025K). A P5 adaptor was added by PCR ...
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bioRxiv - Neuroscience 2020Quote: ... Sequencing libraries (1.3 nM) were chemically denatured and applied to an Illumina NovaSeq flow cell using the NovaSeq XP chemistry workflow (Illumina-20021664). Following transfer of the flowcell to an Illumina NovaSeq instrument ...
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bioRxiv - Neuroscience 2020Quote: ... The cell solutions were immediately transferred to ice and transported to the Wellcome Trust Centre for Human Genetics (WTCHG) for scRNAseq via 10x genomics chromium (10x genomics, US) (Single Cell 3’ v3) and Illumina hiseq 4000 (Illumina, US). This approach achieved 66-72K mean reads per cell and a sequencing depth of 53-55% per cell before filtering.
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bioRxiv - Molecular Biology 2020Quote: ... RNA was extracted and sequence reads (100 bp paired-end) were generated with Illumina TruSeq high output version 3 chemistry on a HiSeq 2500 (Illumina, Inc.) at NRC-Plant Biotechnology Institute (NRC-PBI) ...
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bioRxiv - Cancer Biology 2020Quote: ... TN5 tagmentation and library amplification realized 3’end fragments by Nextera XT DNA Sample Preparation Kit (Illumina, Cat#FC-131-1024) according to the manufacturer’s instructions while P5_TSO and Nextera_N7xx took the place of the custom primers ...
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bioRxiv - Developmental Biology 2022Quote: ... 500 ng of total RNA were used as input for QuantSeq (QuantSeq 3′ mRNA-Seq Library Prep Kit FWD for Illumina; Lexogen) following the autoQuantSeq automated workflow ...
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bioRxiv - Neuroscience 2020Quote: ... Samples were processed according to the standard 10x Chromium 3’ workflow and pooled before sequencing over two lanes (Illumina HiSeq 4000).
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bioRxiv - Cancer Biology 2020Quote: ... 500 ng alkylated RNA was used and prepared with a commercially available kit (QuantSeq 3′ mRNA-Seq Library Prep Kit FWD for Illumina and PCR Add-on Kit for Illumina, Lexogen). Sequencing was performed on an Illumina NovaSeq SP platform in 100bp-single-read mode.
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bioRxiv - Genomics 2021Quote: ... and 1µM Dexamethasone or 0.1% ethanol for 3 hours. Transposition was performed using the OmniATAC protocol (Corces et al. 2017) and the tagment DNA TDE1 enzyme (Illumina, 20034197). DNA was purified using the MinElute PCR purification kit (Qiagen) ...
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bioRxiv - Molecular Biology 2023Quote: ... 1 x 105 mESCs and 5 x 104 S2 cells were resuspended in Tagment DNA Buffer and treated with 3 µL TDE1 Tagment DNA Enzyme (Illumina 20034197). After thorough mixing ...
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bioRxiv - Genomics 2023Quote: For all tandem repeat identification and quantification we used DNA sequences from 2,504 individuals from the Phase 3 of the 1,000 Genomes Project (∼30x coverage Illumina short-read data) (Byrska-Bishop et al ...
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bioRxiv - Immunology 2024Quote: ... 2024) using the command CreateSeuratObject with the following parameters: min.cells = 3 and either min.features = 200 (for Illumina reads from MBC gate) or min.features = 100 (for the Oxford Nanopore reads from the ASC gate) ...
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bioRxiv - Genomics 2023Quote: ... 200ng alkylated RNA were used as input for generating 3’-end mRNA sequencing libraries using a commercially available kit (QuantSeq 3ʹ mRNA-Seq Library Prep Kit FWD for Illumina, Lexogen).
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bioRxiv - Cell Biology 2022Quote: The input samples were submitted to the Washington University Genome Technology Access Center to obtain and sequence the cDNA libraries (10XGenomics, 3’v3.1; Illumina NovaSeq S4) according to established protocols ...
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bioRxiv - Genomics 2024Quote: ... we further pooled the hybrid selection into 3 “super pools.” 2) The hybrid selected libraries were sequenced on a NovaSeq 6000 (Illumina, 20012850) with a S1 Reagent Kit v1.5 (200 cycles ...
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bioRxiv - Cancer Biology 2024Quote: ... Collected single cells were placed on ice and further processed using the Chromium™ Single Cell 3’ Solution (10x Genomics) and sequenced on a NovaSeq 6000 (Illumina) with Illumina 3’ v3.1-paired end chemistry ...
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bioRxiv - Cancer Biology 2024Quote: ... for 15 min at RT with 3 µl of the USER adaptor (NEBNext Multiplex Oligos for Illumina, NEB, cat. no. E7338A) for an additional 15 min at 37°C ...
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bioRxiv - Evolutionary Biology 2021Quote: ... A paired-end library was constructed from 1 µg of the DNA using TruSeq DNA PCR-Free LT Sample Prep kits (Illumina K.K., Tokyo, Japan) following the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2020Quote: ... libraries were prepared from 1 ng of cDNA using the Nextera® XT DNA Library Preparation Kit (Illumina Inc., San Diego, CA USA) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2021Quote: ... and 2.5 µl of each Nextera Index 1 (N7XX) and Nextera Index 2 (N5XX) primers from a Nextera DNA Sample Preparation Index Kit (Illumina, FC-121-1011). PCR was performed according to the following protocol ...
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bioRxiv - Genetics 2020Quote: ... 5 males and 1 female) were used to perform library preparation and sequencing (100 bp single-end) on the HiSeq 4000 sequencer (Illumina, San Diego, CA) following the manufacturer’s sequencing protocols ...
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bioRxiv - Immunology 2021Quote: ... Chromatin fragments bound to magnetic beads were resuspended in 30 μl Tagmentation mix (14 μl H2O, 15 μl Tagment DNA Buffer [Illumina, order no. 15027866], 1 μl Tagment DNA Enzyme [Illumina, order no. 15027865]) and incubated for 10 minutes at 37°C ...
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bioRxiv - Genomics 2021Quote: RNA-Seq library preparation was carried out from 1 µg total RNA using the TruSeq Stranded mRNA kit (Illumina, San Diego, CA, USA), which allows mRNA strand orientation ...
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bioRxiv - Cell Biology 2022Quote: ... the sequencing was performed under the setting of single read 1×51 bp to generate ∼30 million reads per sample on HiSeq 1000 sequencer (Illumina, San Diego, CA). Data analysis was performed as described before (56).
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bioRxiv - Neuroscience 2021Quote: ... ensuring that all sequencing adapters were removed and that the average quality score for each read was above Q20 (representing 1 in 100 Illumina base error rate). Reads which were aligned by HISAT2 v2.0.6 (Kim et al. ...
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bioRxiv - Cancer Biology 2020Quote: ... Libraries were pooled and single end-sequenced (1×75) on the Illumina NextSeq 500 using the High output V2 kit (Illumina, San Diego, CA). Quality control was performed using Fastqc (v0.11.5 ...
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bioRxiv - Neuroscience 2022Quote: ... of the three offspring and their parents was performed at the University of Washington with 1 μg of DNA on a HiSeq 2000 Sequencing System (Illumina, San Diego, CA). The WGS data were aligned using a standard BWA pipeline to the Budgerigar (Melopsittacus undulatus ...
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bioRxiv - Evolutionary Biology 2022Quote: ... We sent 100 ng of DNA from 289 sapsuckers (Supplemental Materials Table 1) to Genome Quebec for sequencing on either an Illumina HiSeq4000 PE150 or the NovaSeqSP 6000 PE150 (Table S1) (Illumina, San Diego, CA).
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bioRxiv - Genomics 2021Quote: RNA-Seq library preparation was carried out from 1 µg total RNA using the TruSeq Stranded mRNA kit (Illumina, San Diego, CA, USA), which allows mRNA strand orientation (sequence reads occur in the same orientation as anti-sense RNA) ...
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bioRxiv - Evolutionary Biology 2021Quote: ... A paired-end library was constructed from 1 µg of the DNA using TruSeq DNA PCR-Free LT Sample Prep kits (Illumina K.K., Tokyo, Japan) following the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2021Quote: ... RNA-seq libraries were generated with 1 μg of RNA as input using the TruSeq RNA Sample Prep Kit (Illumina, San Diego, CA) with poly(A)-enrichment according to the TruSeq protocol ...
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bioRxiv - Genomics 2020Quote: ... 1 nmol of Nimblegen HE universal blocking oligonucleotides and 1 nmol of Nimblegen HE index-specific blocking oligonucleotides (corresponding to the Illumina TS index used) in a 1.5 μl microcentrifuge tube ...
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bioRxiv - Genomics 2020Quote: ... We spiked 12 pM library with 1% PhiX control (PhiX Control v3) and sequenced on an Illumina MiSeq platform (Illumina, San Diego, USA) using a MiSeq Nano Reagent Kit v2 (500 cycles) ...
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bioRxiv - Genomics 2020Quote: ... We spiked the 12pM library with 1% PhiX control (PhiX Control v3) and sequenced on an Illumina MiSeq platform (Illumina, San Diego, USA) using a MiSeq Nano Reagent Kit v2 (500 cycles).
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bioRxiv - Cancer Biology 2023Quote: ... and sequencing error probability 0 ≤ ϵ ≤ 1 (which we set to .01 in our experiments to proxy the per-base sequencing error rate in Illumina sequencing instruments), we can enumerate the likelihood of drawing the observed reads from all possible underlying allele statuses ...
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bioRxiv - Genomics 2023Quote: ... a total of 0.75 ng of DNA was combined with 2.5 µl 1/35th diluted transposome (Illumina; purchased in kit # FC-121-1011), and 1X Tris Buffer (10X Tris Buffer ...
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bioRxiv - Cancer Biology 2023Quote: ... Illumina sequencing libraries with 6-bp single indices were constructed from 1 μg total RNA using the TruSeq Stranded mRNA LT kit (Illumina, RS-122-2101). The resulting library was validated using the Standard Sensitivity NGS Fragment Analysis Kit (AATI ...
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bioRxiv - Immunology 2024Quote: ... paired-end libraries were constructed from 1 µg of the initial genomic material using the TruSeq DNA v2 Sample Prep Kit (Illumina, #FC-121-2001) as per the manufacturer’s instructions ...
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bioRxiv - Microbiology 2022Quote: Sequencing libraries were prepared from 1 ng of each VSG PCR product using the Nextera XT DNA Library Preparation Kit (Illumina, FC-131-1096) following the manufacturer’s protocol except for the final cleanup step ...
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bioRxiv - Plant Biology 2022Quote: Sequencing libraries were generated with 300 ng to 1 μg of RNA using the TruSeq Stranded mRNA Sample Preparation Kit (Illumina, San Diego, CA). The libraries were assessed for size and purity using the Agilent 2100 Bioanalyzer and quantitated by qPCR using a KAPA Biosystems Library Quantitation kit (Roche ...
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bioRxiv - Neuroscience 2023Quote: ... Multiplex samples Pool (1.65 pM including PhiX 1%) was loaded in NextSeq 500/550 High Output v2 kit (75 cycles) cartridge (catalog #FC-404-1005; Illumina, San Diego, CA) and loaded on NextSeq 500 System machine (Illumina) ...
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bioRxiv - Genomics 2023Quote: Poly-A selected stranded mRNA libraries were constructed from 1 μg total RNA using the Illumina TruSeq Stranded mRNA Sample Prep Kits (Illumina, San Diego, CA) according to manufacturer’s instructions with the following exception ...
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bioRxiv - Genomics 2024Quote: ... Genomic sequencing library was prepared from 1 ng of purified DNA using the Illumina Nextera XT library preparation kit (Illumina #FC-131-1096), following the manufacturer’s instructions or with a reduced volume of reagents to 20% ...