Labshake search
Citations for Illumina :
801 - 850 of 1186 citations for 4' Trifluoromethoxy 5 trifluoromethyl 1 1' biphenyl 3 carboxylic acid since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Evolutionary Biology 2024Quote: ... and mate-pair libraries of 3-kb insert size were prepared using the Nextera Mate Pair Sample Preparation Kit (cat. No. FC-132-1001, Illumina). We performed DNA shearing for the fragment and mate-pair library preparations using Covaris S220 ...
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bioRxiv - Cell Biology 2024Quote: ... and 3’ mRNA sequencing libraries were generated according to manufacturer’s recommendations (Lexogen, 015-QuantSeq 3′ mRNA-Seq Library Prep Kit FWD for Illumina and 020-PCR Add-on Kit for Illumina). Samples were analyzed on an Illumina HiSeqV4 SR50 platform by the NGS Facility at Vienna BioCenter Core Facilities (VBCF) ...
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bioRxiv - Immunology 2024Quote: ... Single-cell RNA libraries were prepared according to the 10x Genomics Chromium Single Cell 3′ Reagent Kits v2 User Guide and sequenced on a NovaSeq 6000 (Illumina).
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bioRxiv - Neuroscience 2024Quote: ... Single nuclei were isolated from frozen brain tissue using iodixanol-based density gradient centrifugation and submitted to UCLA Technology Center for Genomics and Bioinformatics for library preparation (via Chromium Single Cell 3’ v3 kit from 10x Genomics) and sequencing (via NovaSeq 6000 S2 platform from Illumina). Cell Ranger (10x Genomics ...
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bioRxiv - Pathology 2024Quote: ... 5’- GTC TCG TGG GCT CGG AGA TGT GTA TAA GAG ACA GGG ACT ACH VGG GTW TCT AAT-3’ (italicized sequence is the Illumina adapter following by barcode ...
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bioRxiv - Genomics 2024Quote: ... Pooled samples were sent to Admera Health and sequenced on a NovaSeq S4 (one pool with 40 samples) or NovaSeq X Plus (3 pools with 18-19 samples) platform (Illumina) using 150 bp paired-end reads.
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bioRxiv - Genomics 2024Quote: ... Sequencing was performed using a molarity of 62.4 nM and 3% PhiX spike in on the Nova Seq 6000 with SP flow cell kit and 200 cycles (Illumina).
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bioRxiv - Cancer Biology 2024Quote: ... H146 and H69) and 3 NSCLC lines (H838, H1944 and A549) was subjected to methylation analysis using the Infinium MethylationEPIC BeadChips (Illumina) allowing the simultaneous quantitative measurement of the methylation status at 865,918 CpG sites ...
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bioRxiv - Cancer Biology 2024Quote: ... Library generation for 10x Chromium analysis was performed following the Chromium Single Cell 3′ Reagents Kits (10x Genomics) and sequenced on an Hiseq4000 (Illumina), to achieve an average of ∼44,000 reads per cell and ∼11,000 cells per sample ...
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bioRxiv - Developmental Biology 2021Quote: ... and the resulting nuclear pellet was resuspended in 5 µl buffer TD (Illumina, San Diego, CA) and combined with 2.5 µl H2O and 2.5 µl Tn5 transposase (Tagment DNA Enzyme ...
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bioRxiv - Developmental Biology 2021Quote: ... 5 μg DNase-treated RNA was used as input for ribosomal depletion using the Epicentre (Illumina) Ribo-Zero ribosomal depletion kit (Cat# SCL24H) ...
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bioRxiv - Molecular Biology 2021Quote: ... The purified mRNA 5′ end libraries were multiplexed again and then sequenced on HiSeq 4000 (Illumina) using paired-end (2x 100 cycles ...
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bioRxiv - Developmental Biology 2022Quote: ... Completed libraries were pooled in an equimolar ratio along with 5% PhilX Control Library V3 (Illumina), denatured and diluted to 2.0pM as per the manufacturer’s instructions ...
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bioRxiv - Evolutionary Biology 2022Quote: ... parental fish were whole-genome sequenced with 5–10X coverage (Illumina Hiseq platforms, BGI Hong Kong). The genotyping of F1 fish was carried out with the DarTseq technology (Diversity Arrays Technology ...
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bioRxiv - Developmental Biology 2022Quote: ... and the resulting nuclear pellet was resuspended in 5 µl buffer TD (Illumina, San Diego, CA) and combined with 2.5 µl H2O and 2.5 µl Tn5 transposase (Tagment DNA Enzyme ...
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bioRxiv - Neuroscience 2023Quote: ... 6 pM of DNA library spiked with .5% PhiX viral DNA was clustered on cBot (Illumina) and then sequenced on a HiScanSQ module (Illumina).
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bioRxiv - Genomics 2024Quote: ... with 5 μM forward and reverse primers in an Eco™ Real-Time PCR system (Illumina). qPCR primers were synthesized as follow ...
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bioRxiv - Genomics 2021Quote: ... Total RNA (1 μg) from each sample was used for NEBNext PolyA selection and Ultra Directional RNA Library preparation (Illumina, E7490, E7420). Prepared libraries were sequenced using 2×150 bp paired-end read chemistry on an Illumina HiSeq 4000 platform.
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bioRxiv - Molecular Biology 2021Quote: ... 1 ug of treated RNA was then input into the Illumina TruSeq Small RNA Library Preparation Kit (Illumina; Cat #: RS-200-0012) with 11 cycles of PCR amplification ...
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bioRxiv - Genomics 2020Quote: ... Samples were prepared for sequencing using 1 ug of genomic DNA following the TruSeq PCR free kit protocol (Illumina, FC-121-3001). Resulting libraries were quality checked on an Agilent DNA 1000 bioanalyzer (Agilent Technologies ...
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bioRxiv - Plant Biology 2020Quote: 42 RNA samples from experiment 1 were used for mRNA-Seq library preparation using the TruSeq Stranded mRNA Library Prep Kit (Illumina, CA, USA). Libraries were multiplexed and single-end 100 nt RNA-Seq was performed on a Novaseq 6000 at the GIGA Center (University of Liege ...
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bioRxiv - Genomics 2022Quote: ... a library was prepared from 1 μg of genomic DNA and a TruSeq DNA PCR-free Sample Preparation Kit (Illumina, CA, USA). Paired-end (151 bp per read ...
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bioRxiv - Genomics 2022Quote: ... Libraries were prepared with 1 μg of total RNA for each sample using a TruSeq Stranded mRNA Sample Prep Kit (Illumina, CA, USA). Paired-end sequencing (151 bp ...
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bioRxiv - Microbiology 2022Quote: ... The V4 region of the 16S rRNA gene was amplified from 1 or 7μl DNA and sequenced with a MiSeq (Illumina, San Diego, CA) using the 500 cycle MiSeq Reagent Kit ...
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bioRxiv - Cell Biology 2020Quote: ... cDNA was diluted 1:15 and subsequently tagmented and amplified for 12 cycles using the Nextera XT DNA Library Preparation Kit (Illumina FC-131). Individual libraries were QC’d by Qubit and Agilent Bioanalyzer ...
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bioRxiv - Genetics 2020Quote: ... One sequencing experiment (Experiment 1) was performed according to the manufacturer’s directions for the TruSeq Small RNA Sample Preparation Kit (Illumina, RS-200-0012). The protocol was adapted to incorporate a 2S blocking DNA oligo for removal of prevalent Drosophila small ribosomal RNA from the sequencing library [67] ...
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bioRxiv - Genomics 2020Quote: ... The cDNA library was generated from 1 µg of total RNA using TruSeq RNA Sample Prep Kit (Illumina, San Diego, CA, USA) following manufacturer’s protocols ...
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bioRxiv - Immunology 2020Quote: ... The Illumina RNA-seq cDNA library was prepared using 1 μg of RNA from each sample and TruSeq V2 kits (Illumina, CA, USA), with reduced RNA fragmentation time (3 minutes ...
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bioRxiv - Plant Biology 2021Quote: ... Libraries for RNA-Seq were prepared from 1 µg of total RNAs with the TruSeq Stranded mRNA Library Prep Kit (Illumina, CA, USA), multiplexed and sequenced in two runs with an Illumina NextSeq500 device (high throughput mode ...
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bioRxiv - Systems Biology 2023Quote: ... 0.5 µL of universal primer and 0.5 µL of index primer (each at 0.5µM concentration; NEBNext multiplex oligos for Illumina index primers set 1 E7335). This mix has been amplified for 15 cycles (same amplification conditions as before) ...
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bioRxiv - Genomics 2023Quote: 1 ng of genomic DNA from each sample was used with Illumina MiSeq Nextera XT DNA Library Preparation Kit (Illumina, Inc., USA), the paired-end library was constructed with an insert (500 bp ...
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bioRxiv - Immunology 2023Quote: ... and sequencing was performed in a 1×100 bp single-read format using a NovaSeq 6000 v1.5 100-cycle reagent kit (Illumina, Cat no. 20028316). Base calling was performed with Illumina Real Time Analysis (RTA ...
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bioRxiv - Evolutionary Biology 2023Quote: ... and g2997a) at the forward primer binding site used in the ‘round 1 PCR’ step below (the step attaching partial Illumina sequencing adaptors), thus excluding PCR amplification of the competitor viruses during sequencing library preparation such that only the library viruses ...
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bioRxiv - Molecular Biology 2022Quote: ... 1 µg of total RNA was used as input for the TruSeq RNA Library Prep Kit v2 (Illumina, Inc., San Diego, CA) and the manufacturer’s protocol was followed ...
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bioRxiv - Neuroscience 2024Quote: ... Illumina Sequencing libraries were prepared using the TruSeq low sample protocol from 1 µg of total RNA (Illumina, San Diego, CA, USA). The resulting libraries were sequenced in paired-end mode ...
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bioRxiv - Genomics 2024Quote: DNA concentration was normalized to 1 ng/μl and subjected to library preparation using Nextera XT DNA Library Preparation Kit (Illumina, CA, USA) according to the manufacturer’s instructions and sequenced with NextSeq550 using Mid Output Kit v2.5 (300 cycles ...
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bioRxiv - Genetics 2023Quote: ... 10 ng of amplicon DNA from PCR reaction 1 was combined with 10 µL of Nextera adapter index primers (Illumina Cat#20027213) and 25 µL Kapa HiFi Hotstart ReadyMix ...
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bioRxiv - Neuroscience 2023Quote: ... Female and male samples after MR1 were sequenced (single-end reads, 1×75 nt) with Illumina NextSeq 500 (Illumina, San Diego, USA). Female samples after MR2 were sequenced (single-end reads ...
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bioRxiv - Immunology 2022Quote: ... Amplified (1 ng) cDNA was used to generate barcoded libraries using the Nextera XT DNA library preparation kit (Illumina, San Diego, CA). The size of the libraries was checked using the Agilent 2100 Bioanalyzer High Sensitivity DNA chip and their concentration was determined using the Qubit® fluorometer (ThermoFisher Scientific ...
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bioRxiv - Genomics 2022Quote: ... we generated a combination of noisy ONT sequencing (MinION R.9.4.1 flow cells with the LSK-109 ligation library prep kit) and Illumina sequencing (PE 150 bp, ∼49x coverage; Illumina NovaSeq 6000). Based on published ONT benchmarking (https://nanoporetech.com/accuracy) ...
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Lysyl oxidase regulates epithelial differentiation and barrier integrity in eosinophilic esophagitisbioRxiv - Cell Biology 2023Quote: ... Sequencing libraries were constructed from total RNA (1 μg) using a TruSeq Stranded mRNA Library Prep (Illumina Inc., San Diego, CA, USA). RNA sequencing was performed on Illumina HiSeq2000 platform ...
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bioRxiv - Genomics 2024Quote: ... Total RNA-seq libraries were generated from 1 μg of DNase-treated RNA using the TruSeq Stranded total RNA library preparation kit (Illumina, Cat# 15031048) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2024Quote: ... Sequencing libraries for Δ2 and Δ3 lines were prepared with 1 μg of gDNA per sample using the TruSeq® DNA PCR-Free Low-Throughput Library Prep Kit (Illumina, 20015962) according to the manufacturer’s directions ...
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bioRxiv - Genomics 2024Quote: ... and 1 μL of TAE polymerase (TruePrep® DNA Library Prep Kit V2 for Illumina®, Cat. no. TD501-02, Vazyme Biotech). PCR thermocycling was carried out in Biometra Tone 96G thermocyclers (Analytik Jena) ...
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bioRxiv - Immunology 2024Quote: ... cDNA was diluted to an average of 200 pg/µl and 100 pg cDNA from each cell was tagmented by adding 1 µl TD and 0.5 µl ATM from a Nextera XT DNA Library Preparation Kit (Illumina: FC-131-1096) to 0.5 µl diluted cDNA in each well of a fresh 384-well plate ...
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bioRxiv - Systems Biology 2024Quote: ... All libraries were dual-indexed with 8 nucleotide indexes using NEBNext® Multiplex Oligos for Illumina® (Index Primers Set 1) and were sequenced on NextSeq 2000 (Illumina) either by 2×150bp or 2×50bp configuration.
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bioRxiv - Microbiology 2024Quote: Indexed sequencing libraries were generated from 1 ng genomic DNA using Nextera XT DNA Library Preparation Kit (Illumina, San Diego, CA, USA) following the protocol of the manufacturer ...
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bioRxiv - Evolutionary Biology 2021Quote: ... Amplicons were calculated for their nano-molarity and diluted to 4 nM (see Illumina 470-2016-007-B) for HTS run on MiSeq sequencer using the Illumina MiSeq Reagent Kit Version 3 (600bp pair-ended) ...
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bioRxiv - Molecular Biology 2021Quote: ... before being diluted to approximately 4 nM for loading onto an Illumina MiSeq (Illumina, San Diego, CA, USA). An Illumina 500 cycle MiSeq Reagent Kit v2 (Illumina ...
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bioRxiv - Genomics 2020Quote: ... and 4 samples were combined into RNA-seq runs on the Illumina MiSeq platform (Illumina, San Diego, CA).