Labshake search
Citations for Illumina :
801 - 850 of 917 citations for Copper;5 10 15 20 tetrakis 4 methylphenyl porphyrin 22 24 diide since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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The spatial landscape of glial pathology and T-cell response in Parkinson’s disease substantia nigrabioRxiv - Neuroscience 2024Quote: ... The libraries were pooled with 10% PhiX spike-in and sequenced with NextSeq High Output 300 Cycles kits (Illumina) on an Illumina NextSeq 550 (read 1 ...
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bioRxiv - Genomics 2024Quote: Sequencing libraries were prepared from 10 -100 ng input DNA using the Nextera DNA flex library prep (Illumina, USA) using IDT for Illumina – Nextera DNA UD Indexes Sets A-D (Illumina ...
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bioRxiv - Genetics 2023Quote: ... 500ng of total RNA with RIN values of 6.5-10 underwent polyA selection and TruSeq library preparation according to instructions provided by Illumina (TruSeq Stranded mRNA LT Kit ...
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bioRxiv - Plant Biology 2023Quote: ... the libraries were diluted to 10 nM and submitted to high-throughput sequencing using the NextSeq 500 system (Illumina) at the IGC Genomics Unit ...
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bioRxiv - Molecular Biology 2023Quote: ... 500 ng of total RNA with RIN values of 7.2-10 underwent polyA selection and TruSeq library preparation according to instructions provided by Illumina (TruSeq Stranded mRNA LT Kit ...
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bioRxiv - Molecular Biology 2023Quote: ... Fragmented genomic DNA libraries were produced from 10 ng of each sample treated with 1.5 µl of Nextera XT tagmentase (Illumina) at 37°C for 45 min with gentle shaking ...
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bioRxiv - Cancer Biology 2024Quote: ... 500 ng of total RNA with RQN values of 9.1-10 underwent polyA selection and TruSeq library preparation according to instructions provided by Illumina (TruSeq Stranded mRNA LT Kit ...
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bioRxiv - Cell Biology 2024Quote: ... 500 ng of total RNA with RIN values of 8.3-10 underwent polyA selection and TruSeq library preparation according to instructions provided by Illumina (TruSeq Stranded mRNA LT Kit ...
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bioRxiv - Cell Biology 2024Quote: ... After 2-3 passages in complete DMEM with 10% FBS gDNA was extracted to confirm the genotype by Illumina amplicon sequencing and live cell microscopy was performed.
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bioRxiv - Genomics 2020Quote: ... The pool was denatured with sodium hydroxide according to Illumina recommendation and spiked with 5% PhiX (PhiX control v3 Illumina Catalogue FC-110-3001) before loading (Fig 1).
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bioRxiv - Microbiology 2020Quote: ... One nanogram of quantified DNA was sheared enzymatically at 55°C for 5 min using the Illumina Nextera XT tagmentation enzyme (Illumina, San Diego, CA, USA). Tagmented DNA fragments were enriched by 10 cycles of PCR amplification using PCR master mix and primers with the index from Illumina ...
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bioRxiv - Genomics 2020Quote: Small RNA libraries were prepared starting from 5 µL DNase treated and spike-in supplemented RNA eluate using a TruSeq Small RNA Library Prep Kit (Illumina, San Diego, CA, USA) according to the manufacturer’s protocol with two minor modifications(1) ...
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bioRxiv - Plant Biology 2020Quote: ... on Thermo Fisher Scientific’s Quantstudio 5 before multiplex pooling (13-16 samples per lane in equal representation) and sequencing on the NextSeq500 platform (Illumina, San Diego, California, USA) paired-end 2×150 bp High Output.
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bioRxiv - Immunology 2021Quote: ... The V(D)J enriched library was then constructed via Chromium Single Cell 5’ Library Construction Kit (10x Genomics Cat#1000020) and libraries were sequenced in a NovaSeq™ 6000 Sequencing System (Illumina, San Diego, CA). V(D)J sequences were collapsed using Cell Ranger ...
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bioRxiv - Genomics 2020Quote: ... Each pool (2nM per library; Agilent SureSelect XT HS, n=6; Agilent SureSelect XT RNA Direct, n=6; Illumina TruSeq RNA Exome, n=5) was sequenced on one lane on the Illumina HiSeq 3000 platform in the Technology Center for Genomics and Bioinformatics at UCLA ...
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bioRxiv - Microbiology 2024Quote: ... and diluted to 5 pM prior to sequencing on an Illumina MiSeq platform with a 2 × 250 bp paired-end protocol (Illumina, San Diego, CA, USA). Sequencing reads were deposited in the European Nucleotide Archive (ENA ...
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bioRxiv - Developmental Biology 2024Quote: ... Pooled libraries with unique 5′ and 3′ index adapter pairs were sequenced on an NextSeq 2000 P3 flow cell (Illumina®: San Diego, CA) to generate 66M (±12M ...
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bioRxiv - Cancer Biology 2021Quote: ... Libraries were pooled and sequenced with 75bp single end sequencing to a depth of 10 × 106 reads on a NextSeq500 (Illumina). Sequencing reads were demultiplexed using bcl2fastq (v2.17.1.14 ...
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bioRxiv - Cancer Biology 2021Quote: ... Nuclei were centrifuged at 500 g for 10 min and immediately resuspended in 25 µl of buffer containing 2.5 µl of Tn5 transposase (Illumina, #15027865) for a 30 min incubation at 37°C ...
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bioRxiv - Developmental Biology 2021Quote: ... 10%(v/v) N,N-dimethyl formamide) and 1 μl of Tagment DNA Enzyme from Nextera Sample Preparation Kit (Illumina) were added to the DNA-beads complex and incubated for 70 sec at 37 °C ...
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bioRxiv - Neuroscience 2021Quote: ... Universal adapters were ligated followed by 10 cycles of PCR using Illumina PCR Primer Cocktail and Phusion DNA polymerase from Illumina. Subsequent library purification with Agencourt AMPure XP beads was validated with Agilent Bioanalyzer 2100 ...
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bioRxiv - Molecular Biology 2021Quote: ... 2.5 µL of 10 µM primer mix (515F forward and 806R reverse rRNA gene V4 primers with Illumina MiSeq adaptors), and 30 ng of sample DNA ...
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bioRxiv - Molecular Biology 2020Quote: For meRIP-Seq RNA-seq libraries were prepared from both eluate and 10% input mRNA using the TruSeq mRNA library prep kit (Illumina), subjected to quality control (MultiQC) ...
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bioRxiv - Cell Biology 2021Quote: ... mRNA was prepared with the QuantSeq 3’ mRNA-Seq Library Prep Kit FWD for Illumina (Lexogen, Vienna, Austria) and sequenced with 10 million 50 bp single-end reads on the HiSeq 2500v4 (Illumina) at Life and Brain GmbH ...
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bioRxiv - Systems Biology 2021Quote: ... GBC sequencing libraries were spiked into transcriptome libraries at 1:10 and sequenced on the NextSeq or MiSeq platform (Illumina). Transcriptome libraries were sequenced separately using a NovaSeq5000 S2 300-cycle kit.
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bioRxiv - Cancer Biology 2020Quote: ... and the analysis was run using sequencing reads of 3 or higher from 454 data and 10 or greater reads from Illumina. Single cell sequencing was performed as described previously (Mann et al. ...
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bioRxiv - Cancer Biology 2022Quote: ... the ADT fraction was amplified for 10 cycles with SI-PCR oligo (10x Genomics) and TruSeq Small RNA RPI-x (Illumina) primers to index the samples ...
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bioRxiv - Cell Biology 2021Quote: ... library construction (250-300 bp insert cDNA library) and RNA sequencing (Illumina HiSeq X Ten, paired-end, 10 M reads). Quality controls were included for the total RNA samples and during the procedures for RNA-sequencing.
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bioRxiv - Microbiology 2020Quote: ... Tagmented DNA fragments were enriched by 10 cycles of PCR amplification using PCR master mix and primers with the index from Illumina. Libraries were quantified by the KAPA SYBR fast quantitative PCR kit (Life Technologies ...
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bioRxiv - Cancer Biology 2021Quote: Clusters were generated by cBot with the library diluted to 10 pM and then sequenced using a NovaSeq 6000 (Illumina). The library construction and sequencing were performed at Shanghai Biotechnology Corporation (Shanghai ...
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bioRxiv - Genomics 2020Quote: ... prepared samples were pooled into two pools (with 10 and 14 individuals each) for sequencing on a HiSeq 4000 (Illumina) using paired-end (PE ...
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bioRxiv - Immunology 2020Quote: Permeabilized cells were pelleted for 10 minutes at 500xg and resuspended in 50 μL of transposition mix (25 μL 2x TD buffer (Illumina), 16.5 μL 1X PBS ...
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bioRxiv - Genomics 2021Quote: ... Bisulfite-treated DNA samples (50 ng) were subjected to 10 cycles of PCR using random primers from the TruSeq DNA Methylation Kit (Illumina) to add adapters ...
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bioRxiv - Genomics 2021Quote: ... [140] with parameters “2 7 7 80 10 100 2000 -ngs -h”. We also used kseek to search for tandem repeats in the male Illumina reads.
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bioRxiv - Developmental Biology 2021Quote: ... 25 mL of 2x NEBNext Ultra II Q5 Master Mix, 3 mL of 10 mM Universal PCR primer from Illumina, 3 mL of 10mM Index PCR primer and 9 mL of nuclease-free water and amplified for 10 cycles (98C for 10 s ...
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bioRxiv - Neuroscience 2021Quote: ... Pooled sequencing libraries were loaded at a concentration of 1.8pM with 10% PhiX spike-in and sequenced using eight Illumina NextSeq 150 Cycle Hi-Output v2.5 kits (Illumina #20024907) on an Illumina NextSeq 550 System ...
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bioRxiv - Immunology 2021Quote: ... and duplicate compression was performed using the Cell Ranger software package (10× Genomics, CA, v2.1.0) and bcl2fastq2 (Illumina, CA, v2.20) according to the manufacturer’s recommendations ...
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bioRxiv - Genomics 2021Quote: ... The multiplexed pool was quantified by qPCR and sequenced (paired-end, 2 x 151 cycles and 10 bp for the index reads) using a NovaSeq 6000 device (Illumina) at 3-fold coverage.
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bioRxiv - Plant Biology 2021Quote: ... using 10 mM of pooled normalized libraries on the cBOT with a TruSeq PE Cluster Kit v3-cBot-HS (Illumina). Subsequently Illumina HiSeq 4000 sequencing was performed to generate the reads.
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bioRxiv - Immunology 2020Quote: ... RNA-seq libraries were prepared from both eluate and 10% input mRNA using the TruSeq mRNA library prep kit (Illumina), subjected to quality control (MultiQC) ...
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bioRxiv - Molecular Biology 2021Quote: ... was used to extract mRNA from 1 μg total RNA and NEB Ultra Library Preparation Kit for Illumina was used to prepare sequencing libraries with different indexing primers to enable pooling of up to 10 libraries per lane of 50 bp SE HiSeq 2000 Sequencer (Illumina) runs ...
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bioRxiv - Microbiology 2020Quote: ... Samples were processed for WGS using multi-segment PCR [10] and then prepared for deep sequencing using the Nextera XT library preparation reagent (Illumina). Samples were sequenced on the Illumina NextSeq platform ...
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bioRxiv - Microbiology 2020Quote: ... paired-end cDNA libraries were prepared from 10 μg of total RNA following poly-A purification using the TruSeq Stranded mRNA kit (Illumina) according to manufacturer’s instructions ...
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bioRxiv - Immunology 2020Quote: ... The transposed DNA was amplified by PCR for 10-12 cycles by using the Nextera DNA Library Preparation Kit and Nextera XT Indexing Kit (Illumina). The library DNA within the 150-to 500-bp range was enriched by AMPure XP beads (Beckman Coulter) ...
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bioRxiv - Immunology 2020Quote: ... The samples were mixed in equimolar ratios to obtain 30 μl of a final concentration of 10-nM and sequenced by Illumina MiSeq (v3 kit ...
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bioRxiv - Cancer Biology 2022Quote: ... Gene expression libraries and barcode enrichment libraries were pooled in an approximately 10:1 molar ratio and libraries were sequenced on the NovaSeq platform (Illumina).
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bioRxiv - Molecular Biology 2022Quote: ... libraries were submitted to the Genomics and Microarray Shared Resource at the University of Colorado Anschutz Medical Campus for sequencing of 10 million paired end reads per sample on the NovaSEQ6000 (Illumina).
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bioRxiv - Genomics 2022Quote: ... Beeline was used to convert raw image (.idat) files to genotype call (.gtc) files.10 The data were analyzed using GenomeStudio® 2.0 software (Illumina). 11
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bioRxiv - Microbiology 2022Quote: ... The 10-pM final library was sequenced on the Illumina MiSeq sequencing platform (2 × 150bp paired-end reads) (Illumina Inc.).
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Neural circuit-wide analysis of gene expression during deafening-induced destabilization of birdsongbioRxiv - Neuroscience 2022Quote: ... of amplified cDNA was added to 10 μL Tagmentation Buffer (Buffer TD from the Nextera XT DNA Sample Prep Kit, Illumina), 1 μL nuclease-free water ...