Labshake search
Citations for Illumina :
8401 - 8450 of 9681 citations for hsa mir 185 Real time RT PCR Detection Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2021Quote: ... The resulting RNA was used to prepare an RNA-seq library using NEBNext Ultra™ Directional RNA Library Prep Kit from Illumina, according to the manufacturer’s instruction ...
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bioRxiv - Microbiology 2020Quote: ... aeruginosa (Pae5 and Pae1505) genomic DNA sequencing libraries were prepared using Nextera DNA library prep kit (Illumina, Cat # FC-121-1031) and utilizing the Nextera DNA Sample Preparation Index Kit (Illumina ...
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bioRxiv - Microbiology 2020Quote: ... Sequencing of the amplicon library was performed on the Illumina MiSeq System with MiSeq reagent kit v2 (Illumina Inc., CA, USA), including 5.0% PhiX as an internal control.
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bioRxiv - Microbiology 2020Quote: ... The final combined library was sequenced using Illumina technology on NextSeq 500 sequencer using mid output 150-bp paired-end (PE) sequencing kit (Illumina, 20024905).
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bioRxiv - Microbiology 2020Quote: ... Experiments were conducted in triplicate and samples sent to Macrogen (Seoul, South Korea) for Ribosomal RNA reduction using Ribo-Zero™□ rRNA Removal kit (Illumina), library preparation using the TruSeqⓇ stranded mRNA kit (Illumina ...
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bioRxiv - Microbiology 2020Quote: ... The libraries were prepared using TruSeq stranded Total RNA sample preparation kit with Ribo-Zero according to the manufacturer’s protocol and sequenced using HiSeq 2500 (Illumina, CA, USA), generating 125-bp paired-end reads (see the RNA-seq analysis workflow in Fig 7).
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bioRxiv - Molecular Biology 2020Quote: Ribosomal RNA was depleted from the total RNA using the Ribo-Zero Gold rRNA removal kit (Human/Mouse/Rat) (Illumina, USA). The ribo-depleted RNA was used to create an ssRNA-Seq library ...
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bioRxiv - Microbiology 2021Quote: ... and sequenced under a 150-bp read length using Illumina HiSeq 4000 systems and HiSeq SBS Kit v4 reagents (Illumina, USA). Read demultiplexing and adapter trimming were performed by the Imperial BRC Genomics Facility using their production pipeline.
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bioRxiv - Systems Biology 2020Quote: ... Final loading concentration of 1.8 pM and 1% PhiX (v/v) was used with the NextSeq® 500/550 High Output Kit v2 (150 cycles) (Illumina). Samples were sequenced paired-end with 75 cycles and single index of 6 cycles.
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bioRxiv - Cancer Biology 2020Quote: ... cell nuclei were extracted using cold lysis buffer (see supplementary methods) and submitted to tagmentation (Nextera DNA Library Preparation Kit, Illumina, USA). After purification ...
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bioRxiv - Genomics 2021Quote: ... from 2 days differentiated TX1072 mESCs was subjected to strand-specific RNA-seq library preparation with the TruSeq® RNA Sample Preparation Kit v2 (Illumina), which included polyadenylated RNA enrichment using oligo-dT magnetic beads ...
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bioRxiv - Genomics 2021Quote: ... The Illumina MiSeq paired-end sequencing service was provided by the Genomics Core (Institute of Molecular Biology, Academia Sinica) using the MiSeq Reagent Nano Kit v2 (MS-103-1003; Illumina, USA). For the reference isolate GV230 ...
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bioRxiv - Genomics 2021Quote: ... Samples were concentrated using a centrifugal evaporator Speed Vac® to a final volume of 5 μl and we started the TruSeq Small RNA Sample Preparation Kit (Illumina) protocol according to manufacturer’s instructions ...
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bioRxiv - Microbiology 2019Quote: ... The samples were used to generate whole transcriptome libraries using the NextSeq 500 high output kit (Illumina, San Diego, CA, USA) with a NextSeq 2000 sequencing instrument (Illumina) ...
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bioRxiv - Genomics 2021Quote: Custom RNA capture-based libraries were prepared starting from 8.5 μL eluate for biofluid samples and 100 ng RNA for FFPE and MAQCA/B using the TruSeq RNA Exome Library Prep Kit (Illumina, USA). Library preparation happened according to the manufacturer’s protocol with some minor modifications ...
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bioRxiv - Genomics 2021Quote: ... Approximately 1000 ng of extracted RNA was used to prepare libraries for sequencing using the TruSeq Stranded mRNA Library Prep Kit (Illumina, 20020594). Libraries were sent for paired-end sequencing on the NovaSeq 6000 (100 bp paired-end reads) ...
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bioRxiv - Genomics 2021Quote: ... Library preparation was done with the Nextera DNA Flex Library Prep Kit following manufacturers’ protocol (Illumina Inc. San Diego, CA, USA) and quality was checked using the Agilent Bioanalyzer 2100 High Sensitivity DNA Kit (Agilent ...
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bioRxiv - Genetics 2020Quote: ... A total of 2 μg RNA was used for mRNA isolated and RNA-seq library construction using the TruSeq RNA Library Preparation Kit (Illumina, USA), according to the manufacturer’s recommendations ...
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bioRxiv - Developmental Biology 2021Quote: ... parental DNA was extracted from one individual male and female from tail-fin clip and sequencing library were synthesized with Nextera XT Kit (Illumina, USA) according to the manufacturer’s protocol ...
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bioRxiv - Genetics 2020Quote: ... we used the NEXTFLEX® Rapid DNA-Seq Kit 2.0 for Illumina paired-end sequencing library (Illumina, San Diego, CA, USA) and the xGen hybridization capture system with Exome Research Panel v2 (Integrated DNA Technologies ...
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bioRxiv - Genetics 2020Quote: ... The Illumina MiSeq platform was used with the shotgun 2 x 250 paired-end sequencing methodology (Nextera DNA Preparation Kit, Illumina, USA). The sample used 0.33 of a flow cell and primer design was optimized for use with Platinum Taq DNA polymerase (Invitrogen ...
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bioRxiv - Evolutionary Biology 2021Quote: Sequencing libraries were prepared from 100 ng DNA using the TruSeq Nano DNA sample preparation kit (cat# 20015964/5, Illumina Inc.) targeting an insert size of 350bp ...
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bioRxiv - Cell Biology 2020Quote: ... Samples with RNA Integrity Number (RIN) >8 were subjected TruSeq Poly-A mRNA Library Pro Kit protocol 15031047 RevD (Illumina, USA) to generate indexed cDNA libraries ...
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bioRxiv - Cell Biology 2020Quote: ... and DNA libraries for sequencing were constructed using Nextera XT DNA Library Prep kit (FC-131-1024, Illumina, San Diego, CA). The 24 prepared libraries were pooled and submitted to Michigan State University Genomics Facility ...
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bioRxiv - Genomics 2021Quote: ... and paired-end sequenced on Illumina HiSeq 2500 platform using TruSeq SBS Kit v3-HS (200 cycle) (Illumina, San Diego, CA) following the manufacturer’s recommendations ...
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bioRxiv - Genomics 2021Quote: ... Libraries were prepared based on a 20X scaled-down Nextera XT DNA Library Prep Kit (Illumina, Cat. No. FC-131-1024) protocol (35 ...
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bioRxiv - Genomics 2021Quote: ... and 8.8 µl of paired i7 and i5 Nextera XT Index Kit V2 indexing primers (Illumina, Cat. No. FC-131-2001) were added to each Tagmentation reaction ...
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bioRxiv - Microbiology 2021Quote: ... Nucleotide sequencing was performed using the TruSeq RNA sample preparation kit in an Illumina HiSeq 2500 instrument (Illumina, San Diego, USA) with a paired-end and 150-base-read protocol in RAPID module ...
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bioRxiv - Neuroscience 2020Quote: ... retinas of WT or Prom1-KO mice and were used to synthesise cDNA libraries with a TruSeq stranded-mRNA library preparation kit (Illumina, 20020594). The libraries were sequenced with the NextSeq 500 platform (Illumina) ...
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bioRxiv - Evolutionary Biology 2020Quote: ... Two DNA pool-seq libraries were prepared according to manufacturer’s protocols using the Illumina TruSeq Nano DNA HT Library Prep Kit (Illumina, California, USA). Briefly ...
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bioRxiv - Cancer Biology 2020Quote: ... Extracted nuclei was processed for TN-5 mediated tagmentation using the Illumina Tagment DNA Enzyme and buffer kit (Nextera Illumina # 20034210) ...
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bioRxiv - Neuroscience 2021Quote: ... Tagmentation by Tn5 was performed using reagents from the Nextera DNA Sample Preparation Kit (FC-121-1030, Illumina; San Diego, CA) as previously described [5] ...
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bioRxiv - Cancer Biology 2021Quote: ... above 9.0 and normalized to 500 ng total RNA for library preparation using TruSeq® stranded mRNA sample prep kit (Illumina, #20020595). After library preparation ...
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bioRxiv - Cancer Biology 2021Quote: ... RNA sequencing libraries were generated using the TruSeq Stranded RNA Library Prep Kit v2 (RS-122- 2001, Illumina, San Diego, CA) and 100 bp paired-end reads were sequenced on an Illumina HiSeq 2500 to at least 26 million reads per sample at the Functional Genomics Core Facility at UCSF ...
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bioRxiv - Immunology 2021Quote: ... 150pg of cDNA was used to generate Illumina compatible sequencing libraries with the NexteraXT library preparation kit (Illumina, San Diego, CA) per manufacturer’s protocols ...
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bioRxiv - Cell Biology 2020Quote: ... libraries for sequencing were prepared from using the TrueSeq RNA Sample Prep Kit v2-Set B (Illumina, San Diego, CA, US) according to the manufacturer’s instructions ...
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bioRxiv - Evolutionary Biology 2020Quote: ... Ribosomal RNAs were depleted from 5 μg of total RNA of the sample by Ribo-Zero rRNA Removal Kit (Seed, Root) (Illumina®). After rRNA depletion ...
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bioRxiv - Evolutionary Biology 2020Quote: ... RNA-Seq libraries were prepared using TruSeq Stranded Total RNA Library Prep Kit with Ribo-Zero Human (RS-122-2201, Illumina Inc.) following manufacturer’s protocol ...
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bioRxiv - Microbiology 2021Quote: ... Amplicon sequencing was performed on an Illumina MiSeq platform using Reagent Kit v2 [2 x 300 cycles] (Illumina Inc., CA, US). Demultiplexing and trimming of Illumina adapters and barcodes was done with Illumina MiSeq Reporter software (version 2.5.1.3).
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bioRxiv - Microbiology 2021Quote: ... Sequencing of the pooled libraries was performed using an Illumina MiSeq benchtop sequencer and 2×250bp paired-end reads MiSeq v2 kit-500 cycles reagents (Illumina, USA). Raw reads were trimmed of remnant sequencing adapters sequences and low-quality regions (Phred score < Q20 ...
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bioRxiv - Microbiology 2020Quote: ... Sequencing of the resulting library was carried out from both ends (2×300 bp) with the 600-cycle MiSeq Reagent Kit v3 on MiSeq (Illumina, USA) and the adapters trimmed from the resulting reads at the facility.
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bioRxiv - Immunology 2020Quote: ... dilution with TruSeq® Stranded mRNA Library Prep for NeoPrepTM kit as per the NeoPrep Library Preparation System Guide on the instrument (Illumina). The libraries were diluted 1:5 (v/v) ...
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bioRxiv - Microbiology 2021Quote: ... Ethanol precipitated samples were resuspended in FPF sample buffer from the TruSeq stranded mRNA-Seq kit reagents (Illumina, San Diego, CA). mRNA abundance was quantified using a Quant-iT RNA Assay kit (Invitrogen ...
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bioRxiv - Microbiology 2021Quote: ... 16S rRNA gene sequencing was performed on normalized pooled amplicons using the Illumina MiSeq System using a MiSeq reagent kit v2 (300 cycles; Cat. No. MS-102-2002, Illumina Inc.). FASTQ files for reads 1 (forward) ...
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bioRxiv - Microbiology 2021Quote: ... Genomic libraries were constructed form the DNA samples using the Nextera XT DNA Sample Preparation Kit (Illumina; San Diego, CA, USA), and paired-end sequencing (2×250 bp ...
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bioRxiv - Immunology 2020Quote: ... Final loading concentration of 1.7 pM and 1% (v/v) PhiX was used with the NextSeq® 500/550 High Output Kit v2 (150 cycles) (Illumina Samples were sequenced paired-end with 75 cycles of each read and single index of 6 cycles.
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bioRxiv - Microbiology 2021Quote: ... at the Centro de Biotecnología Vegetal, Universidad Andrés Bello (Santiago, Chile) using the TruSeq Nano DNA Kit (Illumina Inc., CA, USA.) following the TruSeq Nano DNA Sample Preparation Guide 15041110 Rev ...
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bioRxiv - Immunology 2021Quote: ... Final libraries were pooled and sequenced using a High output single-end 75 cycle (version 2) sequencing kit using the Illumina Nextseq 550 platform (Illumina, USA).
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bioRxiv - Immunology 2021Quote: ... single reads and 7 bases index read on an Illumina HiSeq2000 instrument using the TruSeq SBS Kit HS-v3 (50-cycle, FC-401-3002, Illumina Inc). RNA seq data in FASTQ form from each sample were first quality assessed using fastQC ...
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bioRxiv - Cell Biology 2021Quote: cDNAs were prepared from lysed cells according to SMART-Seq2.0 (Picelli et al., 2014) for low input RNA sequencing and libraries were prepared with Nextera® DNA Library kit (Illumina). Independent biological replicates of each cell type sequenced using Illumina HiSeq2500 and obtained ∼20 million 75 bp single-end reads (7 replicates for β-cells ...