Labshake search
Citations for Illumina :
8401 - 8450 of 9484 citations for Cow Fibrinogen Like Protein 1 FGL1 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2022Quote: We prepared cDNA sequencing libraries for the transcriptome samples using the TruSeq RNA Library Prep Kit v2 (Illumina, San Diego, CA, USA). Paired-end fragment reads were generated on an Illumina NextSeq sequencer using TG NextSeq® 500/550 High Output Kit v2 (300 cycles) ...
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bioRxiv - Synthetic Biology 2022Quote: ... pH 8.5 and sequenced on an Illumina NextSeq 550 following the manufacturer’s instructions using a NextSeq 500/550 Mid or High Output Kit (Illumina, 20024904 or 20024907), or on an Illumina NextSeq 1000 following the manufacturer’s instructions using NextSeq P2 v3 kits (Illumina ...
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bioRxiv - Plant Biology 2022Quote: ... Five micrograms of total RNA from each sample were used to construct an RNA-Seq library by using the TruSeq RNA Sample Preparation Kit according to the manufacturer’s instructions (Illumina, San Diego, CA). A total of 72 libraries (Table S2 ...
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bioRxiv - Molecular Biology 2023Quote: ... 100 000 nuclei were subjected to Tn5 transposition reaction using 2.5 μl TDE1 from Nextera DNA Library Prep Kit (Illumina, #FC-121-1030). After adding the transposition reaction mix ...
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bioRxiv - Plant Biology 2022Quote: ... Total RNAs from WT and e2fabc at 9 DAG were used for construction of cDNA libraries using the TruSeq RNA Library Preparation Kit v2 (Illumina, United States) according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2022Quote: ... Amplified cDNA was fragmented and appended with dual-indexed barcodes using Illumina Nextera XT DNA Library Prep kits (Illumina, San Diego, CA). Libraries were validated on an Agilent 4200 TapeStation (Agilent Technologies ...
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bioRxiv - Molecular Biology 2022Quote: ... Single-read sequencing was performed on a NovaSeq 6000 device using an S2 Reagent kit (100 cycles) according to the manufacturer’s instructions (Illumina Inc., CA, USA).
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bioRxiv - Microbiology 2022Quote: ... enteroids were suspended in RNALater and RNA was isolated using Tru-Seq total RNA Sample Preparation v2 kit (Illumina, Great Abingdon, UK) according to manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2022Quote: ... Complementary DNA libraries from total RNA samples were prepared with Illumina TruSeq RNA preparation kits according to the manufacturer’s instructions (Illumina, San Diego, CA). RNA integrity was assessed using an Agilent 2100 Bioanalyzer (Santa Clara ...
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bioRxiv - Neuroscience 2022Quote: ... Libraries for RNA-seq were prepared using the paired-end TruSeq® Stranded mRNA Sample Preparation kit (Illumina, San Diego, Ca, USA) according to manufacturer’s instructions ...
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bioRxiv - Microbiology 2022Quote: ... 150 pg of cDNA was used to generate Illumina compatible sequencing libraries with the NexteraXT library preparation kit (Illumina, San Diego, CA) per manufacturer’s protocols ...
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bioRxiv - Microbiology 2022Quote: ... following the manufacturer’s instructions and libraries for shotgun metagenomic sequencing were prepared using Illumina DNA Prep kits (Illumina, San Diego, CA, USA). Briefly ...
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bioRxiv - Systems Biology 2024Quote: ... Libraries were then pooled and sequenced with a NextSeq 500/550 Mid Output v2 kit (150 cycles) according to the manufacturer’s recommendation (Illumina, FC-404-2001). Pooled samples were run in triplicate for a minimum sequencing depth of 20 million reads per sample ...
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bioRxiv - Physiology 2023Quote: ... high-sensitivity spectrofluorometric measurement was poly-A selected and reverse transcribed using Illumina’s TruSeq stranded mRNA library preparation kit (Cat# 20020595; Illumina, San Diego, CA). Each sample was fitted with one of 96 adapters containing a different 8 base molecular barcode for high level multiplexing ...
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bioRxiv - Systems Biology 2024Quote: ... the RNA was reverse-transcribed into Illumina shotgun sequencing libraries with TruSeq RNA stranded kit and polyA enrichment following the manufacturer’s protocol (Illumina, San Diego, CA) at the Competence Centre for Genomic Analysis (Kiel ...
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bioRxiv - Cancer Biology 2023Quote: ... 4) tagmentation was performed with 2ng input and sequencing library generated using the Nextera XT library prep kit (Illumina, #FC-131-1024). In short ...
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bioRxiv - Microbiology 2023Quote: ... Library preparation for VLP genomic DNA involved using >25ng input DNA with the Nextera DNA Flex Library Preparation Kit (currently named Illumina DNA prep, Illumina, CA, USA). Deviating from the Illumina protocol’s full reaction volume (50 µL) ...
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bioRxiv - Physiology 2024Quote: ... Sequencing libraries were prepared with Watchmaker DNA Library Prep kits with Fragmentation (Watchmaker, CO, USA) and sequenced using the Illumina NovaSeq instrument (Illumina, CA, USA) with the shotgun sequencing method (a depth of 2 million 2x150 bp read pairs) ...
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bioRxiv - Physiology 2024Quote: ... Based RNA integrity value above 7 determined by the spectrofluorometric measurement RNA samples was poly-A selected and reverse transcribed using Illumina’s TruSeq stranded mRNA library preparation kit (Cat# 20020595; Illumina, San Diego, CA). Library preparation was done for each sample fitted with one of 96 adapters with different 8 base molecular barcode for high level multiplexing and following 15 cycles of PCR amplification ...
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bioRxiv - Evolutionary Biology 2023Quote: ... insert size 550 nt) were constructed from 1.8-2 µg gDNA with the TruSeq DNA PCR-Free kit (Illumina, San Diego, CA) and sequenced on the Illumina HiSeq 2500 by the Genomics Core Facility at Pennsylvania State University ...
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bioRxiv - Microbiology 2024Quote: The Illumina TruSeq Stranded Total RNA Sample Prep Kit with Ribo-Zero Human/Mouse/Rat protocol (Illumina, Inc. San Diego, CA, USA) was used for the following steps ...
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bioRxiv - Neuroscience 2024Quote: ... RNAseq libraries were prepared from 2500 ng total RNA using the TruSeq RNA Library Preparation Kit v2 (Illumina, San Diego, CA, USA) after poly(A ...
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bioRxiv - Neuroscience 2024Quote: ... cDNA libraries were prepared using 200 ng of total RNA according to the manufacturer’s instructions for the TruSeq Stranded mRNA Sample Prep Kit (Illumina, San Diego, CA). The concentration and size distribution of the completed libraries were determined using Qubit fluorometry and an Agilent BioAnalyzer DNA 1000 chip (Santa Clara ...
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bioRxiv - Genomics 2024Quote: ... were used as the input material (approximately 1 ng total quantity) for preparing shotgun metagenome sequencing library with the help of Nextera XT DNA Library Prep Kit (Illumina Inc., USA). Quality of the final library was assesed using high sensitivity D1000 screen tape in 2200 TapeStation (Agilent Technologies Inc. ...
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bioRxiv - Neuroscience 2024Quote: The Total RNA Library was prepared using the Illumina NovaSeq (S4) 300 cycle flow cell and reagent kit (Illumina, San Diego, CA). The high-throughput sequencing strategy included 300 cycles and a read length of paired-end 150 base pairs ...
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bioRxiv - Evolutionary Biology 2024Quote: ... Libraries were made according to the manufacturer’s directions for the TruSeq Small RNA Library Prep Kit (Illumina, Cat. No. RS-200-0012). Resulting libraries were size selected for 145-160bp fragments using Novex 6% TBE gels (Invitrogen ...
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bioRxiv - Genetics 2024Quote: ... Libraries for sequencing were prepared according to the manufacturer’s instructions with TruSeq Stranded Total RNA Library Prep Kit with Ribo-Zero Gold (Illumina, RS-122-2301), and paired-end sequencing was done with a NovaSeq 6000 instrument (Illumina) ...
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bioRxiv - Developmental Biology 2024Quote: ... The DNA extracted from these samples was used for subsequent library construction using the TruSeq Nano DNA LT Library Preparation Kit (Illumina Inc., USA) whose purity and size were validated using an Agilent Bioanalyzer 2100 (Agilent Technologies ...
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bioRxiv - Genomics 2024Quote: ... it enables the observation of all variable sequence elements (spacers, iBARs, and the middle tRNA) with 150-cycle sequencing kits (e.g. Illumina MS-102-3001) and positions spacers and iBARs as early as possible within NGS reads ...
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bioRxiv - Genomics 2023Quote: HMW DNA from 1.5 x 106 cells was used to generate Illumina libraries with the Nextera XT DNA Library Preparation kit (Illumina FC-131-1024) and Illumina DNA PCR-Free Library Prep ...
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bioRxiv - Microbiology 2024Quote: ... Sequencing was performed on an Illumina NovaSeq 6000 instrument using the NovaSeq 6000 S1 Reagent Kit v1.5 (200 cycles) (Illumina, cat. no. 20028317), in a pair-end 2x100 cycle mode ...
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bioRxiv - Immunology 2024Quote: Library preparation was performed starting from 200ng total RNA of each sample using the TruSeq Stranded mRNA Library Kit (Illumina, cat. # 20020595) and the TruSeq RNA UD Indexes (Illumina ...
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bioRxiv - Biochemistry 2023Quote: ... Sequencing was performed on an Illumina NovaSeq 6000 platform with NovaSeq 6000 SP Reagent Kit v1.5 100 cycles (Illumina, cat. no. 20028401). RNA seq experiments were conducted with three independent biological replicates.
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bioRxiv - Microbiology 2023Quote: ... The prepared library was sequenced using a MiSeq sequencing system with a V3 reagent kit (300 × 2 bp; Illumina, 4–6 samples). Lake Biwa viral contigs/genomes (LBVs ...
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Dual RNA-seq identifies proteins and pathways modulated during Clostridioides difficile colonisationbioRxiv - Microbiology 2023Quote: ... Single-end sequencing was performed using a NextSeqR 500/550 High Output Kit v2 (75 cycles) on an Illumina NextSeq™500 system (Illumina, USA). Bacterial controls were sequenced using single-end sequencing with a MiSeq™ v3 cartridge (150 cycles ...
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bioRxiv - Genomics 2023Quote: ... Libraries for sequencing were prepared according to the manufacturer’s instructions with TruSeq Stranded Total RNA Library Prep Kit with Ribo-Zero Gold (Illumina, RS-122-2301) and paired-end sequencing was done with a HiSeq 3000 instrument (Illumina).
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bioRxiv - Microbiology 2023Quote: ... The sequencing libraries were prepared using a modified protocol for the Nextera XT DNA library preparation kit (Illumina Inc. FC-131-1024). The genomic DNA was fragmented ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: Next-generation sequencing library preparations were constructed according to the manufacturer’s protocol (NEBNext® Ultra™ RNA Library Prep Kit for Illumina®). The poly(A ...
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bioRxiv - Microbiology 2023Quote: ... 200 ng of genomic DNA was used as input in the Illumina DNA Prep Kit following the manufacturer’s instructions (Illumina, San Diego, CA). For the in vitro and in vivo experiments (Fig ...
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bioRxiv - Synthetic Biology 2022Quote: ... pH 8.5 and sequenced on an Illumina NextSeq 550 following the manufacturer’s instructions using a NextSeq 500/550 Mid or High Output Kit (Illumina, 20024904 or 20024907), or on an Illumina NextSeq 1000 following the manufacturer’s instructions using NextSeq P2 v3 kits (Illumina ...
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bioRxiv - Evolutionary Biology 2022Quote: ... The final purified (AMPure XP beads) product was then quantified using qPCR according to the qPCR Quantification Protocol Guide (KAPA Library Quantification kits for Illumina Sequecing platforms) and qualified using the TapeStation D1000 ScreenTape (Agilent Technologies ...
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bioRxiv - Molecular Biology 2023Quote: ... DNA libraries for shotgun metagenomic sequencing of viral concentrates were prepared using an Accel-NGS® 1S Plus DNA Library Kit (Swift Biosciences, USA) and sequenced on an Illumina MiSeq platform (Illumina, USA) using Illumina MiSeq Reagent Kit V3 (Illumina ...
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bioRxiv - Neuroscience 2023Quote: ... culminating in a strand-specific total RNA-Seq library created using the VAHTSTM total RNA-Seq v2 Library Prep Kit from Illumina (Vazyme Biotech). The resulting libraries were then sequenced using the Illumina HiSeq 2500 following the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2023Quote: ... mRNA and sRNA libraries were constructed by Illumina trueseq stranded mRNA library preparation kit (Cat. No. 20020594) and TruSeq small RNA sample preparation guide (Illumina, San Diego, CA, United States) as per the manufacturer’s protocol ...
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bioRxiv - Microbiology 2022Quote: ... Each sample’s total DNA was fragmented and tagged with sequencing adapters using Nextera XT DNA Library Preparation Kit (Illumina, San Diego, CA). As previously described ...
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bioRxiv - Cell Biology 2023Quote: ... Pooled libraries were sequenced on an NextSeq500 Sequencer using NextSeq 500/550 High Output Kit v2.5 (Illumina, 20024906, 75 base paired ends).
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bioRxiv - Cell Biology 2023Quote: ... Sequencing adaptors were added by tagmentation and amplified for 12 cycles using the Nextera XT DNA Library Preparation Kit (Illumina, # FC-131). Libraries were pooled at a final concentration of 1.35 pM ...
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bioRxiv - Biochemistry 2023Quote: ... Paired-end sequencing libraries were generated from each dsDNA sample using the Nextera XT DNA Library Preparation Kit (Illumina FC-131-1024) exactly as recommended by the manufacturer ...
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bioRxiv - Cancer Biology 2023Quote: ... The RNA-Seq libraries were sequenced on NextSeq 500 using the NextSeq 500/550 High Output Kit v2.5 (75 cycles) (Illumina, San Diego, CA).
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bioRxiv - Microbiology 2023Quote: ... Preparation of cDNA fragment libraries was performed using the NEBNext® UltraTM II Directional RNA Library Prep Kit for Illumina® (Illumina). Sequencing was carried out on the Illumina NovaSeq platform (10 M paired-end reads ...