Labshake search
Citations for Illumina :
8301 - 8350 of 9484 citations for Cow Fibrinogen Like Protein 1 FGL1 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2021Quote: ... Libraries for sequencing on the Illumina platform were prepared to apply the Nextera XT DNA Library Preparation Kit (Illumina, San Diego, USA) with modifications according to Kishony et al ...
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bioRxiv - Plant Biology 2021Quote: ... The pooled library was sequenced on an Illumina MiSeq platform using a 2× 300-bp MiSeq Reagent Nano Kit v2 (Illumina, CA, USA).
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bioRxiv - Microbiology 2021Quote: ... The final purified product was quantified using qPCR according to the qPCR Quantification Proto-col Guide (KAPA Library Quantification kits for Illumina Sequencing platforms) and qualified using the LabChip GX HT DNA High Sensitivity Kit (PerkinElmer ...
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bioRxiv - Microbiology 2020Quote: ... 2.5 µg of confirmed DNA-free total RNA was treated with Ribo-Zero rRNA Removal Kit for Bacteria according to the manufacturer’s instructions (Illumina, San Diego, CA). Following rRNA removal ...
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bioRxiv - Microbiology 2019Quote: ... The rRNA genes were removed from the total RNA using the Ribo-Zero rRNA removal kit (Illumina, Inc., San Diego, CA, USA) and the remaining mRNA was reverse-transcribed ...
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bioRxiv - Molecular Biology 2021Quote: ... All single cell sequencing libraries were generated using 10x Chromium Next GEM v3.1 kits (10x Genomics) and sequencing was performed on a Novaseq6000 system (Illumina, San Diego, California), using an S2 v1.5 100 cycles flow cell (Illumina ...
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bioRxiv - Microbiology 2020Quote: ... and submitted for DNA sequencing using the MiSeq Reagent kit v3 with the 2 × 300 bp paired-end sequencing protocol (Illumina Inc., USA).
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bioRxiv - Microbiology 2019Quote: ... Dual barcoded Illumina libraries were prepared with the Nextera XT DNA kit according to the manufacturer’s protocol (Illumina, San Diego, CA, USA). Library size distribution was determined using a High-Sensitivity DNA Kit run on a Bioanalyzer 2100 instrument (Agilent Technologies ...
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bioRxiv - Microbiology 2020Quote: ... Metagenomic shotgun libraries were prepared for each sample using the Nextera XT DNA Sample Preparation Kit (Illumina Inc., San Diego, CA, USA) and sequencing was performed on an Illumina NextSeq500 platform with a 2 × 150 bp High Output run ...
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bioRxiv - Cancer Biology 2020Quote: ... MCF10A libraries from three biological replicates of each cell type were prepared using NEBNext® Ultra(tm) RNA Library Prep Kit (Illumina®) and sequenced on HiSeq X by Annoroad Gene Technology Ltd ...
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bioRxiv - Cancer Biology 2021Quote: ... In brief, RNA-seq libraries (non-strand-specific, paired end) were prepared with the NEBNext® Ultra™ RNA Library Prep Kit (Illumina) and were sequenced according to the paired-end 150bp protocol using NovaSeq 6000 ...
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bioRxiv - Cell Biology 2020Quote: ... The remainder of the ATAC-seq library preparation was performed by following manufacturer’s instructions (Nextera DNA Flex Library Prep kit, Illumina, cat. no. 20018704) and the sample quality was validated by BioAnalyzer 2100 (Agilent ...
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bioRxiv - Genetics 2019Quote: RNA-Seq libraries were constructed from 200 ng of total RNA using Illumina TruSeq Stranded Total RNA with Ribo-Zero Gold sample prep kits (Illumina, Carlsbad, CA). Constructed libraries contained RNAs >200 nt (both unpolyadenylated and polyadenylated ...
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bioRxiv - Developmental Biology 2022Quote: ... Samples were processed on the Illumina HiSeq 550 sequencing system with the NextSeq 500/550 High Output kit v2.5 (Illumina, Cat. No. 20024906). The generated fastq files were loaded into the DolphinNext platform (https://dolphinnext.umassmed.edu/ ...
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bioRxiv - Physiology 2022Quote: ... high-sensitivity spectrofluorometric measurement was poly-A selected and reverse transcribed using Illumina’s TruSeq stranded mRNA library preparation kit (Cat# 20020595; Illumina, San Diego, CA). Each sample was fitted with one of 96 adapters containing a different 8 base molecular barcode for high level multiplexing ...
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bioRxiv - Microbiology 2022Quote: ... Bacterial 16S rRNA V4 region was amplified during library preparation via Illumina’s Nextera XT Index Kit v2 (Illumina, Inc.; San Diego, CA) (primers ...
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bioRxiv - Molecular Biology 2022Quote: ... 500 ng of purified RNA in each replicate and condition was used for strand-specific RNA-seq library preparation using the Illumina Stranded mRNA Prep kit (Illumina, Cat. 20040534) and IDT Illumina RNA UD Indexes Set B (Illumina ...
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bioRxiv - Molecular Biology 2022Quote: ... were pooled and RNA extraction/purification was performed for the preparation of sequencing libraries using the Illumina Stranded mRNA Prep kit (Illumina, Cat. 20040534) and IDT Illumina RNA UD Indexes Set B (Illumina ...
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bioRxiv - Cancer Biology 2022Quote: We prepared samples for RNA-seq using the TruSeq Stranded Total RNA Sample Preparation kit with RiboZero Gold (Illumina, San Diego, CA). We performed RNA sequencing for each sample to ∼ 80 million paired-end 150 bp reads on an Illumina NextSeq 500 (Princess Margaret Genomics Centre ...
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bioRxiv - Cell Biology 2022Quote: ... Next generation sequencing library preparations were constructed according to the manufacturer’s protocol (NEBNext® Ultra™ RNA Library Prep Kit for Illumina®). The sequences were processed and analyzed by GENEWIZ (Suzhou ...
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bioRxiv - Cell Biology 2022Quote: ... and stranded total RNA-seq libraries were prepared using the Illumina TruSeq RNA Sample Preparation v2 Kit (Illumina, San Diego, CA, USA) implemented on the liquid handling robot Beckman FXP2 ...
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bioRxiv - Cell Biology 2022Quote: ... RNA libraries were prepared using approximately 100 ng of total RNA according to the manufacturer’s instructions for the TruSeq Stranded mRNA Sample Prep Kit (Illumina, San Diego, CA), employing poly-A mRNA enrichment using oligo dT magnetic beads ...
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bioRxiv - Cell Biology 2022Quote: ... Samples were diluted and 150 pg input cDNA was used with the Nextera XT DNA Library Preparation Kit (Illumina, San Diego, CA) to generate indexed libraries ...
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bioRxiv - Immunology 2020Quote: RNA libraries were prepared using 100 ng of good quality total RNA according to the manufacturer’s instructions for the TruSeq RNA Sample Prep Kit v2 (Illumina, San Diego, CA) employing poly-A mRNA enrichment using oligo dT magnetic beads ...
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bioRxiv - Microbiology 2021Quote: ... metatranscriptomic libraries were prepared directly from total RNA without rRNA removal using the TruSeq RNA Kit (Illumina, Inc., San Diego, CA, USA) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2021Quote: ... commercial reagents were used for unique dual indexed amplicon library generation according to the Artic protocol (NEBNext® ARTIC SARS-CoV-2 Library Prep Kit (Illumina®)) ...
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bioRxiv - Neuroscience 2021Quote: The method used to amplify RNA was adapted from Abruzzi et al (Abruzzi et al., 2015) and was performed using TruSeq® Small RNA Library Prep kit (Illumina, USA) and following the manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: ... Samples were normalized to 1.67 × 105 16S rRNA gene copies/μL prior to barcoding and Illumina MiSeq sequencing (reagent kit v3; Illumina, San Diego, CA) at the University of Minnesota Genomics Center (Minneapolis ...
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bioRxiv - Microbiology 2021Quote: ... The libraries were sequenced using an Illumina MiSeq with MiSeq Reagent Kit v.3 (2x 300 bp, Illumina, San Diego, CA, USA) according to manufacturer’s instructions.
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bioRxiv - Microbiology 2020Quote: ... The final pool was sequenced on Illumina MiSeq with a 2×250bp PE Illumina Reagent Kit v2 (Illumina, Inc., San Diego, CA) in the Biotechnology Center at the University of Wisconsin-Madison
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bioRxiv - Immunology 2020Quote: ... and ligation of the Illumina indexed adapters were performed according to the TruSeq RNA Sample Prep Kit v2 (Illumina, San Diego, CA). The concentration and size distribution of the completed libraries was determined using an Agilent Bioanalyzer DNA 1000 chip (Santa Clara ...
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bioRxiv - Immunology 2020Quote: ... and ligation of the Illumina indexed adapters were performed according to the TruSeq RNA Sample Prep Kit v2 (Illumina, San Diego, CA). The concentration and size distribution of the completed libraries was determined using an Agilent Bioanalyzer DNA 1000 chip (Santa Clara ...
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bioRxiv - Microbiology 2021Quote: ... A single library from these samples (Acerv FL 14120) was prepared using the TruSeq DNA PCR-Free kit (Illumina, San Diego, CA) and the remaining samples were prepared using the TruSeq DNA Nano kit (Illumina) ...
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bioRxiv - Cancer Biology 2021Quote: Strand-specific libraries were generated using the TruSeq Stranded mRNA samples preparation kit (illumine Inc., San Diego, RS-122-2101/2) according to manufacturer’s instructions (Illumina, part #15031047 Rev.E). Briefly ...
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bioRxiv - Cancer Biology 2021Quote: ... Library preparation and RNA-sequencing was performed at the Lausanne Genomic Technologies Facility (LGTF, University of Lausanne; TruSeq Stranded Total RNA Library Kit; Illumina HiSeq 2500). The samples were barcoded and randomized between lanes for sequencing ...
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bioRxiv - Immunology 2022Quote: ... libraries for sequencing were prepared using the NEBNext® Ultra™ II RNA Library Prep Kit for Illumina® following the manufacturer’s instructions (Illumina). Quality of all RNA and library preparations were evaluated with BioAnalyser 2100 (Agilent) ...
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bioRxiv - Genomics 2019Quote: ... The library was then sequenced using the Illumina MiSeq platform with a paired-end 300 bp MiSeq reagent kit v3 (Illumina Inc, USA) at the BecA-ILRI Hub in Nairobi ...
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bioRxiv - Microbiology 2019Quote: ... Bacterial and host mRNA were enriched prior library preparation by using Ribo-Zero Gold rRNA Removal Kit (Epidemiology, Illumina, San Diego, California). The zebrafish genome assembly GRCz11 (http://www.ensembl.org/Danio_rerio ...
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bioRxiv - Immunology 2020Quote: ... 0.1 ng of cDNA from each cell on the plate was individually tagmented using the Nextera XT kit (Illumina, San Diego, CA) in a final volume of 5 µl ...
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bioRxiv - Genetics 2019Quote: ... 0.82 μL TD buffer and 0.08 μL TD enzyme from the Illumina Nextera 24-sample kit (Illumina, Inc., San Diego, CA, USA). Plates were sealed with a Microseal B adhesive plate seal (Bio-Rad ...
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bioRxiv - Genomics 2019Quote: ... Tagmentation of cDNA was performed and sequencing libraries were prepared using the Nextera XT DNA sample preparation kit (Illumina, FC-131-1096) according to the manufacturer’s protocol ...
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bioRxiv - Neuroscience 2019Quote: ... an amount of 2mg of total RNA per sample was processed using Illumina’s RNA Sample Prep Kit following the manufacturer’s instruction (Illumina, San Diego, CA, USA). All single drugs were processed together and multiplexed onto one lane to minimize the batch effect ...
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bioRxiv - Molecular Biology 2019Quote: ... RNA fragments were converted to library using NEBNext® Small RNA Library kit (ref#E7330S, New England Biolabs, UK or equivalent from Illumina, USA) following the manufacturer’s instructions ...
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bioRxiv - Genetics 2019Quote: ... sequencing libraries were generated using oligo-dT magnetic beads from a strand-specific mRNA library preparation kit (TruSeq, Illumina, San Diego, CA), with 17 cycles of PCR amplification ...
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bioRxiv - Genomics 2020Quote: ... 2 µl each of P7 and P5 Nextera XT Index Kit v2 index primers (Illumina Catalogue numbers FC-131-2001 to 2004) were added to each well ...
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bioRxiv - Cancer Biology 2019Quote: ... 50ng of gDNA was used as the input and the sequencing libraries were prepared using the Illumina® Nextera Rapid Capture Enrichment kit (Illumina Inc.). The steps included tagmentation of gDNA ...
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bioRxiv - Genetics 2019Quote: ... All exon regions of all human genes (~22,000) were captured using Agilent SureSelect kits (version C2, December 2018) and sequenced using the NovaSeq platform (Illumina, San Diego, USA). Raw genome sequencing data were analyzed with an in-house software program ...
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bioRxiv - Molecular Biology 2019Quote: ... Sequencing was performed on the Illumina HiSeq 4000 single end 100 bp using the TruSeq SBS Kit HS4000 (Illumina, Inc, California, USA). Reads were aligned to the reference genome (ensembl version 82 ...
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bioRxiv - Molecular Biology 2020Quote: ... pooled and then purified using AMPure beads before running index-PCR using the Nextera XT Index Kit (Illumina, San Diego, CA, USA). AmpliSeq libraries were subsequently normalized before sequencing the libraries as 300bp paired-end reads on Illumina MiSeq (Illumina ...
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bioRxiv - Evolutionary Biology 2020Quote: ... DNA was diluted to approximately 2.5 ng/μL and enzymatically sheared using the Illumina Tagment DNA TDE1 Enzyme and Buffer Kits (Illumina, San Diego, CA) at 55°C for 5 minutes ...