Labshake search
Citations for Illumina :
6901 - 6950 of 7070 citations for WAS Protein Family Member 3 WASF3 Antibody Biotin since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2022Quote: ... a final dilution to 4nM was performed and 13 pM of the final denaturated library was loaded onto the Illumina MiSeq® with 10% PhiX using 2 × 300 bp v3 chemistry (Illumina Inc., CA, USA).
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bioRxiv - Microbiology 2022Quote: ... a paired-end metagenomic library was prepared from 100 ng of DNA using the DNA PCR free Library Prep Kit (Illumina, San Diego, CA, USA) and size selected at about 400 bp ...
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bioRxiv - Neuroscience 2022Quote: ... the mRNA from each brain sample was pooled for RNA-Seq library construction using TruSeq Stranded mRNA LT Sample Prep Kit (Illumina, San Diego, CA, USA). The mRNA library was subjected to paired-end transcriptome sequencing (Illumina platform) ...
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bioRxiv - Microbiology 2020Quote: ... The clustering of the index-coded samples was performed on a cBot Cluster Generation System using TruSeq PE Cluster Kit v3-cBot-HS (Illumina; San Diego, CA, USA), and the library preparations were then sequenced on an Illumina NovaSeq 6000 platform (Illumina ...
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bioRxiv - Microbiology 2021Quote: ... The DNA library was prepared using the Nextera XT library preparation kit with the Nextera XT v2 index kit (Illumina, San Diego, CA, USA). The library fragment length was aimed at fragments with a median size of 575 bases and was assessed with the Genomic DNA ScreenTape assay with the 2200 Tape-Station system (Agilent Technologies ...
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bioRxiv - Neuroscience 2020Quote: DNase treated RNA was subjected to poly-A enrichment and library construction using a RNA-Seq v2 Library Preparation Kit (Illumina, San Diego, CA, USA), followed by 150 bp paired-end sequencing ...
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bioRxiv - Plant Biology 2021Quote: ... An Illumina paired-end DNA library with an insert size of ∼300 bp was constructed using the Genomic DNA Sample Prep kit (Illumina, San Diego, CA, USA) following the manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: ... One nanogram of quantified DNA was sheared enzymatically at 55°C for 5 min using the Illumina Nextera XT tagmentation enzyme (Illumina, San Diego, CA, USA). Tagmented DNA fragments were enriched by 10 cycles of PCR amplification using PCR master mix and primers with the index from Illumina ...
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bioRxiv - Microbiology 2020Quote: ... Next Generation Sequencing (NGS) was performed on an Illumina MiniSeq® using a Mid Output Kit (2×150-cycles) (Illumina, San Diego, CA, USA) by loading 0.8 pM on the flowcell ...
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bioRxiv - Microbiology 2021Quote: ... Sequencing was carried out using the Illumina technology using a standard flow cell and 500 cycle v2 reagent cartridge (Illumina Inc., Hayward, California, USA).
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bioRxiv - Cancer Biology 2020Quote: ... 534-850ng of total RNA with DV200% varying from 38-74% was used for ribosomal depletion and library preparation using the TruSeq Stranded Total RNA LT Kit (Illumina catalog # RS-122-1202) according to manufacturer’s instructions with 8 cycles of PCR ...
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bioRxiv - Neuroscience 2020Quote: ... paired-end sequencing was performed on an Illumina NextSeq 500 sequencer using a 150 bp sequencing kit (v2) (Illumina Inc., San Diego, CA, USA) to produce a minimum of 50M paired-reads per library ...
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bioRxiv - Systems Biology 2022Quote: ... Paired-end sequencing was conducted using a V3 Miseq reagent kit (600 cycles) on an Illumina Miseq instrument (Illumina Inc., San Diego, CA, USA).
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bioRxiv - Genetics 2022Quote: ... Pooled RPFs were diluted to 100 ng/uL and 1 ug was used as input for rRNA depletion using RiboZero Gold (part of Illumina 20020598, San Diego, CA) per manufacturer protocol ...
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bioRxiv - Microbiology 2019Quote: ... a 20 pM pooled library was subjected to paired-end (2×250 bp) high-throughput sequencing on an Illumina MiSeq platform (Illumina, San Diego, CA, USA) using MiSeq reagent kit v2 ...
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bioRxiv - Molecular Biology 2019Quote: ... and 16 PCR positive controls) was sequenced on an Illumina MiSeq® using a MiSeq Reagent Kit v3 (600-cycle) (Illumina, Inc, CA, USA). Raw sequence reads were demultiplexed using a custom Python script ...
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bioRxiv - Systems Biology 2019Quote: ... 600 pg of each cDNA library was fragmented and amplified for sequencing with the Nextera XT v2 DNA Library Preparation kit (Illumina, San Diego, CA, USA). Single-cell libraries at 1.8 pM (final average insert sizes ranging from 580 to 680 bp ...
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bioRxiv - Evolutionary Biology 2019Quote: The Illumina TruSeq Stranded total RNA preparation kit was used to make cDNA libraries from the RNA of each individual whitefly as described by the manufacturer (Illumina, San Diego, CA, USA). Libraries were sent to Macrogen ...
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bioRxiv - Microbiology 2019Quote: ... and 1 ng/µl of DNA was used to prepare DNA libraries using the Nextera XT Library Preparation Kit and Nextera XT v2 Indexes (Illumina, San Diego, CA, USA). Multiplexed libraries were sequenced using paired end 150 bp chemistry on the NextSeq 500 NCS v2.0 (Illumina) ...
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bioRxiv - Immunology 2019Quote: ... The V4 variable region of the 16S rRNA gene was amplified using universal bacterial primers(Klindworth et al., 2013) which were uniquely barcoded for each sample (Illumina Nextera Indexes Version 2). The amplicons were purified ...
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bioRxiv - Systems Biology 2020Quote: ... 1µg total RNA was used for library preparation with the TruSeq Stranded mRNA Library Prep Kit High Throughput (Cat# RS-122-2103, Illumina, San Diego, CA, USA). Libraries were quality-checked on the Fragment Analyzer (Advanced Analytical ...
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bioRxiv - Developmental Biology 2020Quote: ... 1 µg of total uterine RNA was sent to the NIH Intramural Sequencing Center to create a libarary using the TruSeq RNA Kit (Illumina, San Diego, CA, USA) following the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2019Quote: ... 1µg total RNA was used for library preparation with the TruSeq Stranded mRNA Library Prep Kit High Throughput (Cat# RS-122-2103, Illumina, San Diego, CA, USA). Libraries were quality-checked on the Fragment Analyzer (Advanced Analytical ...
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bioRxiv - Microbiology 2020Quote: ... Metagenomic DNA was sequenced at the Joint Genome Institute (JGI) (Walnut Creek, CA) on the Illumina HiSeq 2500 platform (Illumina, San Diego, CA, U.S.A.), which produces 2 x 150 base pairs (bp ...
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bioRxiv - Microbiology 2020Quote: ... The clustering of the index-coded samples was performed on a cBot cluster generation system using HiSeq PE Cluster Kit v4-cBot-HS (Illumina, San Diego, California, USA) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: ... following the manufacturer’s instructions and Whole Genome Sequencing was performed using Illumina Miseq platform with a 2 x 250 paired-end run after Nextera XT library preparation (Illumina Inc., San Diego, CA).
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bioRxiv - Microbiology 2021Quote: ... Shotgun metagenomics and isolates genome sequencing was performed on the NextSeq 500 using NextSeq High Output v2 Kit (300 Cycles) (Illumina, San Diego, CA, USA) by the Sequencing Core Facility at The Novo Nordisk Foundation Center for Biosustainability (Technical University of Denmark ...
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bioRxiv - Microbiology 2021Quote: ... High-throughput amplicon sequencing covering the V4-V5 region of the 16S rRNA gene was performed on an Illumina Miseq 2 × 300 paired-end platform (Illumina, San Diego, CA USA) using the universal primers 515F (GTGYCAGCMGCCGCGGTAA ...
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bioRxiv - Cancer Biology 2019Quote: ... 100 ng of RNA with DV200 >30% was used to prepare sequencing libraries in accordance with the TruSeq RNA Access protocol (Illumina, Inc., San Diego, CA). First strand cDNA was synthesized using random primers followed by second strand synthesis ...
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bioRxiv - Developmental Biology 2019Quote: ... Approximately 5 ug of total RNA was used to deplete ribosomal RNA according to the manuscript of the Ribo-Zero™ rRNA Removal Kit (Illumina, San Diego, USA). After removing ribosomal RNAs ...
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bioRxiv - Genetics 2021Quote: ... multiplexing library preparation with a uniquely tagged 6-bp sequence index was performed following the standard Illumina library construction protocol (Illumina, San Diego, California, USA). The libraries with average insert size 250-300 bp were sequenced using an Illumina Novaseq sequencer ...
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bioRxiv - Evolutionary Biology 2021Quote: ... Next-generation sequencing was performed in a 150-bp paired-ends-read mode using three lanes on a HiSeq3000 sequencer (Illumina, San Diego, CA, USA) at the Get-Plage core facility (Genotoul platform ...
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bioRxiv - Neuroscience 2021Quote: ... The clustering of the index-coded samples was performed on an acBot Cluster Generation System using TruSeq PE Cluster Kitv3-cBot-HS (Illumina, San Diego, CA, USA). The library preparations were then sequenced on an Illumina Hiseq platform ...
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bioRxiv - Microbiology 2021Quote: ... and the pooled DNA was used for the preparation of sequencing libraries using the Nextera XT DNA library prep kit (Illumina, San Diego, CA, USA) as per manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: ... Equimolar normalization of each pool and sequencing was performed at the BYU DNA Sequencing center on a partial 2 × 250 lane (v2) of a HiSeq 2500 (Illumina, Inc., San Diego, CA). Laser complexity was assured by including at least 10% of each lane with shotgun sequencing libraries for other bacterial genomes ...
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bioRxiv - Genomics 2021Quote: ... For long read sequencing (Oxford Nanopore Technologies) total RNA was extracted as described above and depleted using the Ribo-Zero rRNA Removal Kit Plant Leaves (Illumina, San Diego, CA, USA). RNA was then purified and concentrated on a RNA Clean Concentrator™-5 column (Zymo Research ...
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bioRxiv - Cell Biology 2020Quote: RNA concentration and quality was assessed with Ribogreen and QC length profiling by the facility before library generation (TruSeq® Illumina mRNA (Poly(A) enrichment ...
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bioRxiv - Genomics 2020Quote: ... BS-converted DNA (100 ng) of each sample was used for preparing the BS-seq library via the TruSeq DNA Methylation Kit (Illumina, EGMK91324, San Diego, CA). The quality of the libraries was checked using Qubit assays (Q32850 ...
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bioRxiv - Genomics 2019Quote: ... Library preparation was conducted according to the Illumina protocol and sequenced (96-plex) on an Illumina HiSeq 2000 platform (Illumina, San Diego, CA, USA) using 100 bp paired-end reads ...
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bioRxiv - Microbiology 2021Quote: WGS was performed at the Australian Centre of Ecogenomics (University of Queensland, St Lucia, QLD, Australia) on the NextSeq 500 (Illumina, San Diego, CA, USA) with Nextera DNA Flex libraries (Illumina).
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bioRxiv - Microbiology 2021Quote: Paired-end RNA sequencing (RNA-seq) was performed by the I2BC platform (UMR9198, CNRS, Gif-sur-Yvette) on a NextSeq instrument (Illumina, San Diego, CA, USA) using the NextSeq 500/550 High Output Kit v2 (75 cycles ...
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bioRxiv - Molecular Biology 2020Quote: ... 13 pM library was loaded on the Illumina MiSeq® with 10% PhiX using a 2 × 300 bp V3 chemistry (Illumina Inc., CA, USA).
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bioRxiv - Molecular Biology 2021Quote: ... Libraries for WGS were prepared using Nextera XT DNA Library Prep Kit and sequencing was performed on the Illumina NextSeq500 machine as per manufacturer’s protocol (Illumina Inc., San Diego, California, USA) producing 2 x 151 base pair reads.
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bioRxiv - Immunology 2021Quote: ... Library preparation was done using a NEBNext Ultra II Directional RNA Library Prep Kit for Illumina with poly-A selection (Illumina, San Diego, CA, USA) following the manufacturer’s instructions ...
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bioRxiv - Genomics 2021Quote: ... Paired-end sequencing (250 bp) was performed for each library on a MiSeq instrument using 500 cycles v2 chemistry cartridges (Illumina, San Diego, CA, USA).
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bioRxiv - Genetics 2020Quote: ... The two resulting PCR products were mixed in equimolar amounts and each sample was barcoded according to the Nextera XT library preparation protocol (Illumina, San Diego, CA, USA). Sequencing was performed on the Illumina MiSeq platform (DNA Core Facility ...
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bioRxiv - Cell Biology 2022Quote: ... an average of 500 pg of amplified cDNA was used to prepare the libraries following Nextera XT DNA kit (Illumina Ref. FC-131-1096). Libraries were multiplexed by 6 on a high-output flow cells ...
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bioRxiv - Cell Biology 2022Quote: ... as described in the section “Quantitative real-time PCR.” The library was prepared using the TruSeq stranded mRNA sample prep kit (Illumina, San Diego, CA, USA) according to the manufacturer’s instructions and sequenced on an Illumina NovaSeq 6000 platform in the 101 bp single-end mode ...
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bioRxiv - Genomics 2022Quote: ... A total of 100 ng of RNA was used as input to the mRNA sequencing library prep kit (TruSeq RNA Access Library Prep Kit by Illumina, San Diego, CA, USA) according to the protocols of the manufacturer ...
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bioRxiv - Genomics 2022Quote: ... 2 μg of Rohu-1 genomic DNA was used with an Illumina TruSeq DNA PCR-free Library Prep Kit (Illumina, San Diego, CA, USA) to create an Illumina sequencing library ...