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Citations for Illumina :
601 - 650 of 1646 citations for 5 Bromo 2 Tert Butyldimethylsilyl 4 Methylthiazole since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2021Quote: ... and 5’-end RNA-seq library preparation using tagmentation-based modified Nextera XT DNA sample Preparation kit (Illumina). Libraries were tagged with a plate-specific i7 index and were pooled for sequencing on an Illumina NextSeq2000 platform ...
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bioRxiv - Cancer Biology 2022Quote: ... and 5’-end RNA-seq library preparation using tagmentation-based modified Nextera XT DNA sample Preparation kit (Illumina). Libraries were tagged with a plate-specific i7 index and were pooled by batches of 4-9 plates for sequencing on an Illumina NextSeq550 platform ...
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bioRxiv - Microbiology 2021Quote: ... 5 ng of DNA was used as input to create Illumina sequencing libraries using the Nextera kit (Illumina). The samples were pooled and sequenced on an Illumina NextSeq 500 High Output system to obtain 2×150 bp reads ...
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bioRxiv - Bioengineering 2021Quote: ... Additional adapters at 5’-end (P5 and SP1) and 3’-end (P7 and SP2) were designed by Illumina for sequencing purpose ...
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bioRxiv - Immunology 2021Quote: ... Cells were then lysed and fragmented in a single reaction (12.5 µl 2X Illumina TDE buffer, 2.5 µl 1% Tween-20, 2.5 µl 0.2% Digitonin, 5 µl water, 2.5 µl Illumina TDE1 enzyme). Samples were incubated at 37°C for 60 minutes and purified using the Zymo DNA Clean and Concentrator-5 Kit (Zymo) ...
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bioRxiv - Plant Biology 2020Quote: ... custom adapters for selecting 5’-P mRNAs and primers from the TruSeq Small RNA Sample Preparation Kit (Illumina) for multiplexing the libraries as indicated in Zhai et al (2014) ...
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bioRxiv - Cell Biology 2020Quote: ... 5 μl Tagment DNA Enzyme and 20 μl nuclease free water (Nextera DNA Sample Preparation Kit, Illumina, UK) for 45 min at 37°C ...
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bioRxiv - Microbiology 2021Quote: ... The library was diluted to a final concentration of 7 pM and 5% of PhiX DNA (Illumina, USA) was added ...
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bioRxiv - Microbiology 2021Quote: ... Amplicon libraries were mixed with 5% PhiX and sequenced with four MiSeq reagent kits v2 500 cycles (Illumina). A blank extraction kit control ...
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bioRxiv - Immunology 2021Quote: ... The 5’ gene expression libraries were sequenced following 10X Genomics read length guidelines on the NovaSeq6000 sequencer (Illumina).
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bioRxiv - Systems Biology 2023Quote: ... approximately 5×104 cells were lysed and transposed reactions were carried out using Tn5 Transposase (Illumina, #FC121-1030) at 37 °C for 30 min ...
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bioRxiv - Microbiology 2022Quote: ... 2.5 µL primer P5 (5 µM) and 2.5 µL Index 1 primer (N7**, Illumina, CN FC-131-2001) was used for the PCR enrichment ...
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bioRxiv - Systems Biology 2022Quote: ... were lysed and transposed simultaneously in 25 µl of transposition mix (0.1% NP40, 0.1% Tween-20, 0.01% digitonin and 5% Tn5 enzyme (Illumina # 15027865) in Tagment DNA Buffer (FC-121–1030 ...
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bioRxiv - Microbiology 2023Quote: ... Fastq files for 5′ libraries sequenced with the extended R1 strategy were generated using bcl2fastq v2.20.0 (Illumina, Inc). Extended R1 fastqs were then separated into “pseudo R1” fastqs ...
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bioRxiv - Cell Biology 2024Quote: ... The amplified DNA was then pooled to a 10 nM final concentration followed by a 5% PhiX (Illumina) spike and sequenced in a PE100 run on a HiSeq 4000 sequencer (Illumina ...
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bioRxiv - Genomics 2024Quote: ... 5 PCR-cycles for the addition of indexes) according to manufacturer’s instructions (Illumina, Inc., San Diego, CA, USA). The final library pool was quantified by qPCR (Mascher et al ...
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bioRxiv - Plant Biology 2024Quote: ... Libraries were then sequenced with a depth of 5 million of reads using a NextSeq 500 System (Illumina).
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bioRxiv - Cancer Biology 2021Quote: ... The captured libraries were sequenced to achieve a mean unique coverage of at least 1000x using Illumina v.4 chemistry and paired-end 100-base pair (bp) reads (HiSeq, Illumina).
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bioRxiv - Cancer Biology 2021Quote: ... The flow cell underwent cluster amplification and massively parallel sequencing by synthesis using Illumina v.4 chemistry and paired-end 100-bp reads (Illumina).
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bioRxiv - Immunology 2021Quote: Paired-end RNA-Sequencing was performed on two independent biological replicates of 10 or 12 pooled 4-dpf larval zebrafish per genotypic group (nlrc3l mutants versus their siblings) by Illumina HiSeq 2×100 basepairs ...
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bioRxiv - Microbiology 2020Quote: ... A total of 4 pools were sequenced on the Illumina MiSeq using the 600-cycle MiSeq Reagent Kit V3 (Illumina). Each pool contained 3 identical samples to assess sequencing run variations.
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bioRxiv - Microbiology 2020Quote: ... Libraries diluted to 4 nM were sequenced with NextSeq 500 High Output Kit v2 (75 cycles) on a NextSeq 500 (Illumina). Small RNA libraries have been submitted to the NCBI sequence read archive (SRA ...
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bioRxiv - Genomics 2021Quote: ... Nuclei were then collected by centrifuging at 500 x g for 10 min at 4°C and nuclei were resuspended in 50 μL of transposition reaction mix [25 μL 2X TD buffer (Illumina), 16.5 μL PBS ...
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Enhancer Remodeling Promotes Tumor-Initiating Activity in NRF2-Activated Non-Small Cell Lung CancersbioRxiv - Cancer Biology 2020Quote: ... 4 μg of total RNA from A549 cells was subjected to rRNA removal using the Ribo-Zero Gold kit (Illumina). cDNA sequencing libraries were then prepared using the SureSelect Strand-Specific RNA library preparation kit (Agilent Technologies ...
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bioRxiv - Genomics 2020Quote: ... The normalised samples (4 nM) were denatured with 0.2 N NaOH and diluted 20 pM using pre-chilled Hybridisation Buffer (HT1) (Illumina, USA). The 20 pM transcriptome libraries were further diluted to 10 pM with pre-chilled HT1 buffer prior to whole transcriptome sequencing on a MiSeq platform.
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bioRxiv - Genomics 2021Quote: Final samples for both inverse PCR and UMI-amplicons (concentration 4 nM) were sequenced as paired-end reads on HiSeq2500 and NextSeq500 sequencers (Illumina).
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bioRxiv - Genomics 2023Quote: ... Lysate was centrifuged for 10 min at 500g at 4°C and then resuspended in transposition buffer (25 ul TD buffer and 2.5 ul TD enzyme (Illumina, 20034197), 16.5 ul PBS ...
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bioRxiv - Immunology 2024Quote: ... After centrifugation at 500ξ g for 5min at 4°C, the cell pellets were resuspended in tagmentation-mix (containing Tagment DNA buffer, Tagment DNA Enzyme (Illumina), Digitonin (Promega ...
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bioRxiv - Developmental Biology 2022Quote: ... stage-matched comparisons comprising a total of 5 control and 4 Mesp1 KO embryos were ultimately re-pooled and sequenced together for deep sequencing on a NovaSeq6000 S4 lane (Illumina). All libraries were sequenced to depths of at least 24,000 median fragments per cell ...
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bioRxiv - Evolutionary Biology 2022Quote: ... Each pool was sequenced 100 bp single-end on two flow cells of a HiSeq2500 sequencer using the HiSeq SBS sequencing kit version 4 (Illumina). Sequencing was conducted in two separate HiSeq runs to yield enough coverage per sample and later combined ...
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bioRxiv - Genomics 2022Quote: ... We centrifuged at 500 x g for 10 minutes at 4°C and resuspended cells in the transposition reaction mix (25 µL 2X TD buffer (Illumina), 2.5 µL TDE1 Tn5 transposase (Illumina) ...
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bioRxiv - Cancer Biology 2023Quote: ... RNA was collected for 4 biological replicates within each cell line and libraries were prepared by TruSeq mRNA Library Prep (Illumina). The libraries were sequenced on the Illumina HiSeq 4000 platform ...
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bioRxiv - Molecular Biology 2024Quote: ... and finally diluted to 1.5 pM before loading at a low cluster density (4 x 105 clusters / mm2) together with 50% of PhiX spike-in (Illumina). For libraries sequenced on NextSeq extra purification and removal of adapter dimers was performed using SPRIselect beads (Beckman Coulter ...
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bioRxiv - Neuroscience 2024Quote: ... were pelleted at 500 g for 10 minutes at 4°C in a fixed angle centrifuge and resuspended in 50 μL transposition buffer (prepared from Illumina kit components TD buffer and 100 nM transposase [Illumina] ...
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bioRxiv - Neuroscience 2024Quote: ... were pelleted at 500 g for 10 minutes at 4°C in a fixed angle centrifuge and resuspended in 50 μL transposition buffer (prepared from Illumina kit components TD buffer and 100 nM transposase (Illumina) ...
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bioRxiv - Cancer Biology 2021Quote: ... Libraries were sequenced on a HiSeq X Ten using paired-end 2×150 bp sequencing (Illumina). Reads were aligned to the human reference genome (GRCh38 ...
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bioRxiv - Cancer Biology 2021Quote: ... indexed adapter ligation and PCR amplification—using the TruSeq RNA v.2 Library Preparation kit (Illumina). The captured library was pooled with other libraries ...
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bioRxiv - Microbiology 2020Quote: ... 2×75 bp paired-end sequencing was carried out using an Illumina NextSeq 500 system (Illumina), resulting in an average of 12 million reads per sample ...
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bioRxiv - Systems Biology 2022Quote: ... we sorted 500-1000 clonally expanded HLA-A2/YFV NS4b-specific CD8+ T cells directly into 22.5μl of ATAC-buffer (12.5μl 2× TD Buffer (Illumina), 0.5μl 1% Digitonin (Promega G9441) ...
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bioRxiv - Neuroscience 2021Quote: ... resuspended in 25 μl of 1X transposition buffer containing 2 μl of Tagment DNA Enzyme (Illumina) and incubated at 37°C for 30 min in a thermocycler ...
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bioRxiv - Molecular Biology 2021Quote: ... Sequencing was performed on an Illumina MiSeq using a V3 (2×300) MiSeq Reagent Kit (Illumina). An average of 7 million reads from each sample were obtained from the sequencing run ...
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bioRxiv - Genomics 2020Quote: ... RNA sequencing libraries were prepared using the ScriptSeq version 2 kit (Illumina, San Diego, CA, USA). 2 × 75 bp paired end sequencing reads were obtained using an Illumina HiSeq 2500 ...
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bioRxiv - Molecular Biology 2021Quote: ... Sequencing of paired-end reads (75 bp × 2) was performed using the NextSeq 550 system (Illumina). Raw sequence reads were de-multiplexed and trimmed for adapters by using Illumina bcl2fastq conversion software (v2.19) ...
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bioRxiv - Genomics 2021Quote: ... Paired-end sequencing was completed using 300-cycle MiSeq Reagent Kits v2 (Illumina; 2 × 150 cycles).
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bioRxiv - Microbiology 2020Quote: ... Samples were then sequenced with 2×250 bp cycles of v2 Miseq chemistry (Illumina, CA, USA).
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bioRxiv - Microbiology 2021Quote: ... Pair-end sequencing (2× 150-bp) was conducted using the HiSeq X Ten sequencing platform (Illumina) at the Beijing Genomics Institute (Shenzhen ...
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bioRxiv - Microbiology 2021Quote: ... using a MiSeq Reagent Kit v2 and 2 x 151 bp paired-end sequencing protocol (Illumina).
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bioRxiv - Cancer Biology 2020Quote: ... Sequencing was performed on an Illumina MiSeq using the 2 × 300 paired-end chemistry kit (Illumina). Reads were quality-filtered and passed through a proprietary allele calling algorithm and analysis pipeline using the latest IMGT HLA allele database (Robinson et al. ...
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bioRxiv - Evolutionary Biology 2020Quote: ... Hi-C libraries were sequenced on a NextSeq 550 sequencer (2×75 bp, paired-end Illumina NextSeq with the first ten bases acting as barcodes50).
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bioRxiv - Microbiology 2020Quote: 2×251 paired end sequencing was conducted on the Illumina MiSeq platform (Illumina, San Diego, CA) using a Nextera XT kit (Illumina ...