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Citations for Illumina :
5801 - 5850 of 7070 citations for WAS Protein Family Member 3 WASF3 Antibody Biotin since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2021Quote: ... cDNA library was prepared by using NEB Next Ultra RNA Library Prep Kit for Illumina (New England Biolab, Cat# E7530) and whole genome sequencing was performed by Miseq (Illumina).
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Enhancer Remodeling Promotes Tumor-Initiating Activity in NRF2-Activated Non-Small Cell Lung CancersbioRxiv - Cancer Biology 2020Quote: ... 4 μg of total RNA from A549 cells was subjected to rRNA removal using the Ribo-Zero Gold kit (Illumina). cDNA sequencing libraries were then prepared using the SureSelect Strand-Specific RNA library preparation kit (Agilent Technologies ...
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bioRxiv - Neuroscience 2021Quote: Tagmentation and library preparation using 600 pg of cDNA from each plate of cells was then performed with a modified Nextera XT (Illumina) protocol ...
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bioRxiv - Immunology 2021Quote: ... and duplicate compression was performed using the Cell Ranger software package (10x Genomics, CA, v2.1.0) and bcl2fastq2 (Illumina, CA, v2.20) according to the manufacturer’s recommendations ...
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bioRxiv - Immunology 2021Quote: ... and duplicate compression was performed using the Cell Ranger software package (10× Genomics, CA, v2.1.0) and bcl2fastq2 (Illumina, CA, v2.20) according to the manufacturer’s recommendations ...
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bioRxiv - Genetics 2021Quote: ... and ribosomal RNA was depleted from 5 ug of total RNA using the Ribo-Zero™ Gold Kit (Illumina, Inc.). Depleted mRNA was fragmented and converted to first strand cDNA ...
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bioRxiv - Genomics 2022Quote: ... Paired-end sequencing (80 bp from each end) was then performed on the NextSeq 500 system (Illumina, San Diego, CA) at a sequencing depth of 13–19 million reads per library ...
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bioRxiv - Cancer Biology 2021Quote: ... and purified RNA was delivered to GENEWIZ (South Plainfield, NJ) for mRNA preparation with the TruSeq RNA V2 kit (Illumina) and 150bp paired-end sequencing on the Illumina HiSeq2500 ...
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bioRxiv - Cancer Biology 2020Quote: A total of 1 μg of total RNA was used to create libraries with Illumina TruSeq Stranded Total RNA Library Prep Kit (Illumina) according to manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2021Quote: ... rRNA was depleted using Ribo-Zero™ Magnetic Gold Kit for rRNA depletion (Human/Mouse/Rat) (Epicentre for Illumina #MRZG12324) according to manufacturer’s guidelines ...
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bioRxiv - Developmental Biology 2021Quote: ... The resulting 10 nM pool was denatured to 10 pmol with 5% PhiX spike-in and sequenced as single-read on HiSeq 2500 (Illumina) in rapid mode for 51 cycles (plus 7 cycles index read ...
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bioRxiv - Developmental Biology 2021Quote: ... The sequencing library was sequenced at the CCHMC DNA Sequencing core on the HiSeq 2500 sequencer (Illumina, San Diego, CA) using one flow cell of paired-end 75 bp reads ...
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bioRxiv - Cell Biology 2020Quote: ... 1 ug of DNA was processed according to manufacturer instructions (Paired-End DNA sample Prep Kit – Illumina – PE-930-1001), except that DNA was ligated to custom-made adapters for 4 hours at RT ...
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bioRxiv - Genetics 2021Quote: ... high-throughput exome sequencing was performed on a whole-exome library created from Tvrm266 genomic DNA on a HiSeq 2000 Sequencing System (Illumina) as previously described [98] ...
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bioRxiv - Microbiology 2021Quote: A total of 1□ng of dsDNA was used to prepare sequencing libraries using the Nextera XT DNA kit (Illumina). Libraries were analysed on a High Sensitivity DNA Chip on the Bioanalyzer (Agilent Technologies) ...
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bioRxiv - Microbiology 2021Quote: ... Paired-end sequencing was performed using a HiSeq 4000 instrument (2 x 150 bp) as recommended by the manufacturer (Illumina). Raw reads were trimmed with Trimmomatic version 0.36 (Bolger et al ...
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bioRxiv - Immunology 2020Quote: ... 1 ng of cDNA was used to prepare a standard NextEra XT sequencing library (NextEra XT DNA library prep kit and index kits; Illumina). Barcoded Illumina sequencing libraries (NextEra ...
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Rbx1 Cullin-Ring ligase E3 and Ube2m neddylation E2 differentially governs the fitness of Treg cellsbioRxiv - Immunology 2020Quote: ... Approximate 1.5pM of the library for each sample was loaded onto a NextSeq 500 system and sequenced using High Output sequencing kits (75× 2bp) (Illumina). After discarding the cells with high mitochondrial gene ...
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bioRxiv - Microbiology 2019Quote: ... Ribosomal RNA (rRNA) was then depleted from each sample (5 µg each) using the bacterial Ribo-Zero rRNA Removal Kit (Illumina). The integrity of the RNA was evaluated using an RNA 6000 Nano LabChip and an Agilent 2100 Bioanalyzer (Agilent Technologies ...
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bioRxiv - Neuroscience 2021Quote: ... Sequencing was carried out by running 150 cycles on Illumina HiSeq 2500 using TruSeq Rapid SBS Kit (FC-402-4001, Illumina) according to the manufacturer’s instructions.
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bioRxiv - Microbiology 2020Quote: Each fraction was sequenced at the UC Davis Genome Center on an Illumina HiSeq 3000 (Illumina Inc., Hayward, California, USA) with paired-end libraries prepared with the Kapa Hyper protocol and a read length of 150 bp.
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bioRxiv - Microbiology 2020Quote: ... the 309 bp sequence of the nodD coding region was amplified by PCR from the DNA extractions and the PCR products were sequenced by Illumina MiSeq 2×250 bp technology as described in Methods S5 ...
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bioRxiv - Microbiology 2020Quote: ... One ng of the chromosomal DNA obtained was used in a library preparation using the Nextera XT library preparation kit (Illumina) according to manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: ... ribosomal RNA (rRNA) was depleted from the total RNA extracts using the Ribo-Zero Gold (human-mouse-rat) kit (Illumina) following the manufacturer’s instructions ...
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bioRxiv - Immunology 2021Quote: ... 1 ng of cDNA was used to prepare a standard Nextera XT sequencing library (Nextera XT DNA sample preparation kit and index kit, Illumina). Whole-transcriptome amplification and sequencing library preparations were performed in a 96-well format to reduce assay-to-assay variability ...
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bioRxiv - Microbiology 2021Quote: ... Total 5 μg of RNA was used for rRNA depletion by using Ribo-Zero™ (Epicentre, Illumina, Madison, WI USA) kit and purified by using Qiagen-RNeasy miniElute (Qiagen GmbH ...
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bioRxiv - Immunology 2021Quote: ... Amplified cDNA was fragmented and appended with dual-indexed bar codes using the Nextera XT DNA Library Preparation kit (Illumina). Libraries were validated by capillary electrophoresis on an Agilent 4200 TapeStation ...
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bioRxiv - Immunology 2020Quote: ... High-quality RNA (approximately 500 ng; RIN 8) was used for nondirectional paired-end mRNA library preparation (TruSeq Sample Preparation Kit; Illumina).
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bioRxiv - Immunology 2020Quote: ... One ng of pre-amplified cDNA was used to generate barcoded Illumina sequencing libraries (Nextera XT library preparation kit - Illumina) in 8 μl reaction volume ...
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bioRxiv - Microbiology 2021Quote: ... 5 µg of DNase-treated total RNA was subjected to ribosomal RNA removal (RiboZeroTM rRNA Removal for Gram-positive bacteria, Illumina). rRNA depleted samples were then subjected to RNA fragmentation using the Ambion RNA fragmentation kit (AM8740) ...
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bioRxiv - Microbiology 2021Quote: ... rRNA was removed using Ribo-Zero rRNA Removal Kit (Human/Mouse/Rat) and Ribo-Zero rRNA Removal Kit (Bacteria) (Illumina). In order to reduce the impact of the host transcriptome on subsequent analysis ...
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bioRxiv - Immunology 2020Quote: ... 0.3 to 0.5 ng of pre-amplified cDNA was used to generate barcoded Illumina sequencing libraries (Nextera XT library preparation kit, Illumina) in an 8μL reaction volume ...
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bioRxiv - Immunology 2020Quote: ... Sequencing for all other samples was performed according to a 50 bp single-end strategy using the HiSeq2500 sequencer (HiSeq SBS Kit v4; Illumina). Post-sequencing ...
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bioRxiv - Neuroscience 2020Quote: ... and ultra-deep whole genome sequencing (average 30X read-depth across the genome) was performed on a HiSeq X Ten System (Illumina). We developed an efficient data processing and quality control pipeline ...
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bioRxiv - Genetics 2021Quote: WGS of constitutional DNA was performed using the Illumina HiSeq platform as described previously.1 Targeted MBD4 mutation screening was performed (i) using a custom MIP capture panel and sequenced on a NextSeq500 (Illumina) system ...
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bioRxiv - Microbiology 2021Quote: ... The clustering of the index-coded samples was performed on a cBot cluster generation system using HiSeq PE Cluster Kit v4-cBot-HS (Illumina) according to the manufacturer’s instructions ...
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bioRxiv - Genomics 2021Quote: ... Single and paired-end sequencing (150 to 250 cycles, V2 kits) was done on an Illumina MiSeq instrument with automatic adapter trimming (Illumina). Raw reads in fastq format that passed filters were used for analysis using CLC Genomics Workbench 12.0 (Qiagen) ...
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bioRxiv - Genomics 2021Quote: ... The 20 ul preamp was then mixed with 25 ul TD buffer and 5 ul TDE1 buffer (Nextera kit, FC-131-1096, Illumina) and incubated for 5 min at 55 C and held at 10 C ...
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bioRxiv - Genomics 2021Quote: An in-silico genome with synthetic next-generation sequencing reads was generated with the ART software (ART-Illumina version 2.5.8)36 ...
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bioRxiv - Genomics 2021Quote: ... Genomic DNA library was prepared and sequenced using a TruSeq DNA PCR-Free Library Prep Kit (Illumina, San Diego, CA).
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bioRxiv - Genomics 2021Quote: ... DNA was PCR amplified for a total of 11-13 cycles using barcoded primers (Illumina Nextera XT Index Kit v2) and purified using Ampure beads (1.4:1 beads:sample ratio) ...
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bioRxiv - Genomics 2021Quote: ... 200 ng of total RNA was used to construct a TruSeq strand specific library with the Ribo-Zero protocol (Illumina), and all samples were sequenced on a HiSeq2000v3 to a minimal target of 180 million paired-end reads ...
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bioRxiv - Genomics 2021Quote: ... Library preparation was performed over two batches using the Illumina TruSeq RNA Sample Preparation Kit v2 (RS-122-2001 & -2002, Illumina). Samples were sequenced to 50 base pairs ...
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bioRxiv - Genomics 2020Quote: Library preparation for bulk RNA-seq was performed using the Illumina TruSeq Stranded mRNA Sample Preparation Kit (except for biopsy 01115149-TA, which was prepared using the unstranded Illumina TruSeq RNA Sample Preparation protocol (Revision A ...
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bioRxiv - Genomics 2020Quote: ... RNA-seq was performed by single-end sequencing with 51 cycles in high-output mode on a HiSeq 2500 sequencing system (Illumina) and with at least 20 million reads per sample ...
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bioRxiv - Genomics 2020Quote: ... the total DNA was sheared to ~350 bp fragments and the libraries were generated using TruSeq Nano DNA HT Sample preparation Kit (Illumina) following the manufacturer’s instructions ...
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bioRxiv - Genomics 2020Quote: ... which was sequenced on the Illumina NextSeq 500 using PE 150 bp High Output Kit (Illumina, San Diego, California, USA), at Georgia Genomics and Bioinformatics Core (University of Georgia ...
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bioRxiv - Genomics 2020Quote: ... An RNA-seq transcriptome library was prepared using the TruSeq™ RNA sample preparation Kit from Illumina (San Diego, CA) and sequencing was performed on an Illumina Novaseq 6000 by the Shanghai Majorbio Bio-pharm Biotechnology Co. ...
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bioRxiv - Genetics 2020Quote: ... Samples were multiplexed and paired-end sequencing was performed using either a MiniSeq or NovaSeq 6000 (Illumina; San Diego, CA).
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bioRxiv - Genetics 2020Quote: ... Single-cell library was pooled with equal volumes and sequenced using Nextseq500/550 High Output Kit v2.5 (Illumina, cat# 20024906) on Nextseq500/550 sequencer (Illumina ...