Labshake search
Citations for Illumina :
451 - 500 of 9201 citations for hsa mir 185 Real time RT PCR Detection Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genomics 2020Quote: ... we constructed a sequencing library (insert size of 500 bp) with TruSeq DNA PCR-Free Library Prep Kit (Illumina, San Diego, CA, USA) to sequence on HiSeqX (Illumina) ...
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bioRxiv - Microbiology 2021Quote: ... Next the amplicons were checked for their size and purity on a 1.5 % agarose gel and if suitable subjected to the index PCR using the Nextera XT Index Kit v2 Set C/D (Illumina, FC-131-2003). After index PCR the samples were cleaned again with AmPure XP Beads (Beckman Coulter Life Science Cat ...
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bioRxiv - Genomics 2021Quote: ... Genomic DNA libraries for short- and long-read sequencing were generated with the TruSeq DNA PCR-Free Kit (Illumina, San Diego, CA, USA) and the SMRTbell Express Template Prep Kit 2.0 (PacBio ...
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bioRxiv - Genomics 2019Quote: ... The barcode sequencing libraries were subjected to a final bead clean-up SPRIselect reagent and quantified by quantitative PCR (KAPA Biosystems Library Quantification Kit for Illumina platforms; KK4824). Sequencing libraries were loaded on an Illumina sequencer with 2 × 50 paired-end kits using the following read length ...
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bioRxiv - Neuroscience 2020Quote: ... cDNA libraries with an average size of 1.5-2 kb were tagmented and indexed during a second PCR amplification step with the Illumina Nextera XT DNA preparation kit (Illumina, FC-131-1024). Tagmentation was performed according to the manufacturer’s protocol with an input of 500 pg cDNA and amplicon tagment mix for 5 min at 55 °C ...
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bioRxiv - Epidemiology 2019Quote: ... The second round of PCR added the Illumina specific sequencing adapter sequences and unique indexes using the Nextera XT Index Kit v2 (Illumina, San Diego, CA) and KAPA HIFI HotStart Ready Mix (Kapa Biosystems ...
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bioRxiv - Evolutionary Biology 2021Quote: ... A paired-end library was constructed from 1 µg of the DNA using TruSeq DNA PCR-Free LT Sample Prep kits (Illumina K.K., Tokyo, Japan) following the manufacturer’s instructions ...
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bioRxiv - Evolutionary Biology 2020Quote: ... Two micrograms of MDA-amplified DNA were used to generate genome libraries using a TruSeq DNA PCR-Free Kit following the manufacturer’s protocol (Illumina, San Diego, CA, USA). The resulting libraries were sequenced using a Rapid-Run on an Illumina HiSeq 2500 to obtain 100 bp paired-end reads ...
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bioRxiv - Genetics 2019Quote: ... Paired-end sequencing libraries were constructed with an insert size of 500 bp using TruSeq DNA PCR-Free kit (Illumina, San Diego, CA) according to Illumina library preparation protocols ...
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bioRxiv - Genomics 2021Quote: ... The PCR products (4 nM from every single cell) were treated with the Miseq Reagent Kit v2 50 Cycles (Illumina KK, Tokyo, Japan) and sequenced by the MiSeq sequencer (26 bp ...
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bioRxiv - Microbiology 2021Quote: ... Two micrograms of MDA-amplified DNA were used to generate genome libraries using a TruSeq DNA PCR-Free Kit following the manufacturer’s protocol (Illumina, San Diego, CA, USA). The resulting libraries were sequenced using a Rapid-Run on an Illumina HiSeq 2500 to obtain 100 bp paired-end reads ...
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bioRxiv - Microbiology 2021Quote: ... The PCR products (4 nM from every sample) were treated with the MiSeq Reagent Kit v2 (50 cycle format; Illumina KK, Tokyo, Japan) and sequenced using the MiSeq Sequencer (26 bp ...
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bioRxiv - Microbiology 2022Quote: Sequencing libraries were prepared from 1 ng of each VSG PCR product using the Nextera XT DNA Library Preparation Kit (Illumina, FC-131-1096) following the manufacturer’s protocol except for the final cleanup step ...
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bioRxiv - Neuroscience 2022Quote: ... at the HIHG or The American Genome Center at Uniformed Services University of the Health Sciences (USUHS) using the TruSeq DNA PCR-Free library preparation kit and sequencing on the Novaseq 6000 (Illumina, San Diego, CA). WGS was analyzed through a bioinformatics pipeline including alignment to the GRCh38 genome build with BWA mem ...
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bioRxiv - Genomics 2022Quote: ... Libraries were created from DNA of the same HzStark_Cry1AcR male using the TruSeq DNA PCR-Free Low Throughput Library Prep Kit with TruSeq DNA UD Indexes (Illumina, San Diego, CA, USA) to yield a standard paired end library with a 350±50 bp insert size ...
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bioRxiv - Zoology 2022Quote: ... Twelve 150 bp paired-end libraries (six for the Mamm01 primer set and six for the MiFish-U primer set) were prepared with an Illumina TruSeq DNA PCR-free LT Sample Prep kit (Illumina, San Diego, California), spiked with 8% phiX ...
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bioRxiv - Genomics 2023Quote: ... The tagmentation and library preparation was performed with the Nextera DNA Library Preparation Kit with 8 cycles of PCR amplification (Illumina #FC-121-1030).
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bioRxiv - Genetics 2023Quote: ... Sequencing libraries of individual DNA samples were prepared using the Illumina TruSeq DNA PCR-free LP kit (Illumina, San Diego, California, United States) in combination with the IDT for Illumina TruSeq DNA UD indexes kit (Illumina) ...
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bioRxiv - Immunology 2024Quote: ... Sequencing of the PCR amplicons spanning the MHC loci was performed on the Illumina MiSeq platform using a v2 500-cycle kit (Illumina, San Diego, CA) in the FHCC Genomics Core Facility or the UCI-Fred Hutch Cancer Centre Laboratory.
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bioRxiv - Genomics 2019Quote: ... 1μL SR RT Primer and following the manufacturers protocol (NEB small RNA for Illumina library prep); “Hybridize the reverse transcription primer” followed by “ligate 5’ SR adaptor…” ...
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bioRxiv - Microbiology 2023Quote: ... 4 μl of the 3’-5’-adapter-ligated RNA was mixed with barcoded RT primers (Illumina) and cDNA synthesis was performed using SuperScript™ II Reverse Transcriptase (Invitrogen ...
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bioRxiv - Genomics 2020Quote: ... fragment libraries (average fragment sizes of ∼560 bp) were constructed using TruSeq DNA PCR-Free LT Library Prep Kit (Illumina, San Diego, CA, USA). Paired-end sequences of 150 bp long were determined using HiSeq X (Illumina) ...
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bioRxiv - Molecular Biology 2019Quote: The extracted genomic DNA from a laboratory-reared individual was used in constructing a high-throughput sequencing library with 500-bp insert size using the Illumina TruSeq DNA PCR-Free HT Library Prep Kit (Illumina, San Diego, CA, USA). The prepared library was sequenced on an Illumina Hiseq4000 Sequencer using the Hiseq Reagent Kit v3 (Illumina ...
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bioRxiv - Microbiology 2021Quote: ... An average 350-bp paired-end libraries were prepared from extracted genomic DNA by TruSeq DNA PCR-Free High Throughput Library Prep Kit (Illumina, San Diego, CA, USA). Pair-end sequencing (2× 150-bp ...
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bioRxiv - Plant Biology 2021Quote: Libraries for PCR-free short read sequencing were prepared from MN106-Ref genomic DNA using the TruSeq DNA PCR-Free Low Throughput Library Prep Kit (Illumina, San Diego, CA, USA) in combination with TruSeq DNA Single Indexes Set A (Illumina ...
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bioRxiv - Genomics 2021Quote: ... Eight whole genome sequencing libraries with a median insert size of 550 bp were constructed using the Illumina TruSeq DNA PCR-free sample preparation kit (Illumina, San Diego, CA, USA), according to manufacturer’s protocols ...
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bioRxiv - Microbiology 2022Quote: ... a paired-end metagenomic library was prepared from 100 ng of DNA using the DNA PCR free Library Prep Kit (Illumina, San Diego, CA, USA) and size selected at about 400 bp ...
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bioRxiv - Molecular Biology 2019Quote: ... and 16 PCR positive controls) was sequenced on an Illumina MiSeq® using a MiSeq Reagent Kit v3 (600-cycle) (Illumina, Inc, CA, USA). Raw sequence reads were demultiplexed using a custom Python script ...
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bioRxiv - Microbiology 2019Quote: ... the amplicon libraries underwent a second PCR reaction to attach dual indices and Illumina sequencing adapters using the Nextera XT index kit (Illumina, San Diego, CA, USA). Following purification (as described above ...
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bioRxiv - Pathology 2020Quote: The V3-V4 hypervariable regions of the 16S_rDNA were amplified by two steps PCR using v1 primers (Vaiomer) and sequenced using MiSeq Reagent Kit v3 (2×300 bp Paired-End Reads, Illumina, San Diego, CA, USA) as previously described [24] ...
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bioRxiv - Genomics 2021Quote: ... libraries with an average insert size of 450 bp were prepared by the University of Utah’s Huntsman Cancer Institute High-Throughput Genomics Core Facility using the Illumina TruSeq DNA PCR-Free Library Prep Kit (Illumina, San Diego, California, USA). 2 × 150 bp reads for each parent were generated on an Illumina NovaSeq 6000 instrument using the NovaSeq S2 reagent kit (Illumina ...
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bioRxiv - Genomics 2020Quote: ... Paired-end sequencing libraries were constructed with an insert size of 500 bp using a TruSeq DNA PCR-Free kit (Illumina, San Diego, CA, USA) according to Illumina library preparation protocols ...
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bioRxiv - Genomics 2022Quote: ... 2 μg of Rohu-1 genomic DNA was used with an Illumina TruSeq DNA PCR-free Library Prep Kit (Illumina, San Diego, CA, USA) to create an Illumina sequencing library ...
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bioRxiv - Microbiology 2022Quote: ... Purified PCR products were fragmented and tagged with the indexed adaptors using the Nextera DNA XT Sample Preparation Kit (Illumina, San Diego, CA, USA). The sequence library was quantified by using the Quant-iT PicoGreen dsDNA Assay Kit (Invitrogen) ...
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bioRxiv - Cancer Biology 2021Quote: ... Three biological replicates were sequenced for each time point (Illumina HiSeq 2500). On average ...
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bioRxiv - Cell Biology 2020Quote: ... Lentiviral sgRNA inserts were amplified in a two-step PCR (with Illumina adapters added on the second PCR), as previously described39 ...
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bioRxiv - Evolutionary Biology 2021Quote: ... 100bp TruSeq DNA PCR-free libraries (Illumina) were prepared according to manufacturer’s instructions ...
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bioRxiv - Genomics 2022Quote: ... TruSeq DNA PCR free (Illumina Inc., USA) and Collibri PCR free PS DNA (ThermoFisher ...
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bioRxiv - Immunology 2021Quote: ... 25 μl PCR master mix (Nextera, Illumina) and 5 μl indexed amplification primers59 (0.125 μM final concentration ...
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bioRxiv - Genomics 2023Quote: ... 100bp TruSeq DNA PCR-free libraries (Illumina) were prepared according to manufacturer’s instructions ...
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bioRxiv - Genomics 2020Quote: ... After A-tailing and adaptor ligation fragments that carry adaptors on both ends were enriched by 8 cycles of PCR (Illumina TruSeq Nano DNA Sample Kit). The final library was quantified using PicoGreen (Quant-iT ...
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bioRxiv - Microbiology 2019Quote: ... A library was prepared by adaptor ligation with the PCR primer pairs using the TruSeq Nano DNA Library Prep Kit (Illumina, Inc., San Diego, CA, USA). When two or more bands were detected using 1.5%-agarose gel electrophoresis ...
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bioRxiv - Immunology 2020Quote: ... before fragmenting the samples to 450bp fragments and processing with the Illumina TruSeq DNA PCR-Free Sample Preparation kit (Illumina Inc., San Diego, CA, USA). Over the three-year duration of the sequencing phase of the project ...
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bioRxiv - Microbiology 2021Quote: ... A library was prepared by adaptor ligation with the PCR primer pairs using the TruSeq Nano DNA Library Prep Kit (Illumina, Inc., San Diego, CA, USA). When two or more bands were detected using 1.5%-agarose gel electrophoresis ...
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bioRxiv - Cell Biology 2022Quote: ... Tagmentation products were amplified by PCR by adding 1.25 µl of each N and S primers (Illumina Nextera XT 96-index kit FC-131- 1002) and 3.75 µl of NPM solution and using the following thermocycler settings ...
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Legionella relative abundance in shower hose biofilms is associated with specific microbiome membersbioRxiv - Microbiology 2023Quote: ... The second PCR (adaptor PCR) was performed with limited cycles to attach specific sequencing Nextera v2 Index adapter (Illumina). After purification ...
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bioRxiv - Cell Biology 2022Quote: ... 10μl of the tagmented chromatin was mixed with 2.5μl of Nextera PCR primer cocktail and 7.5μl of Nextera PCR master-mix (Illumina FC-121-1030) in low-binding PCR tubes ...
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bioRxiv - Microbiology 2024Quote: ... The second PCR (adaptor PCR) was performed with limited cycles to attach specific sequencing Nextera v2 Index adapter (Illumina). After purification ...
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bioRxiv - Genomics 2019Quote: ... and reverse transcribed using 25 pmol RT primer (5’-AATGATACGGCGACCACCGAGATCTACACGTTCAGAGTTCTACAGTCCGA-3’) for TRU-seq barcodes (RP1 primer, Illumina). A portion of the RT product was removed and used for trial amplifications to determine the optimal number of PCR cycles ...
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bioRxiv - Microbiology 2020Quote: ... A second PCR was performed to attach dual indices and Illumina® sequencing adapters using the Nextera XT Index kit (Illumina®; San Diego, CA, USA). The conditions of this PCR included ...